Virulence genes and genetic relationship of L. monocytogenes isolated from human and food sources in Brazil

Detalhes bibliográficos
Autor(a) principal: Almeida,Rosana Macedo de
Data de Publicação: 2017
Outros Autores: Barbosa,André Victor, Lisbôa,Rodrigo de Castro, Santos,André Felipe das Mercês, Hofer,Ernesto, Vallim,Deyse Christina, Hofer,Cristina Barroso
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Infectious Diseases
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000300282
Resumo: ABSTRACT The herein presented assay provided a bacteriological and molecular characterization of 100 samples of L. monocytogenes isolated from human (43) and food (57) sources, from several regions of Brazil, and collected between 1975 and 2013. Antigenic characterization defined 49% of serotype 4b samples, followed by 28% of serotype 1/2b, 14% of serotype 1/2c, 8% of serotype 1/2a, and 1% of serotype 3b. Both type of samples from human and food origin express the same serotype distribution. Multiplex PCR analysis showed 13 strains of type 4b with the amplification profile 4b-VI (Variant I). Virulence genes hly, inlA, inlB, inlC, inlJ, actA, plcA, and prfA were detected in all samples, highlighting a deletion of 105pb on the actA gene in 23% of serotype 4b samples. Macrorestriction profile with ApaI at PFGE showed 55 pulsotypes, with the occurrence of the same pulsotype in hospitalized patients in São Paulo in 1992 and 1997, and two other highly related pulsotypes in patients hospitalized in Rio de Janeiro in 2008. Recognized pulsotypes in listeriosis cases have also been detected in food. Thus, the prevalence of a serotype and the persistence of certain pulsotypes herald future problems.
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spelling Virulence genes and genetic relationship of L. monocytogenes isolated from human and food sources in BrazilListeria monocytogenesListeriosisVirulenceFoodPFGEBrazilABSTRACT The herein presented assay provided a bacteriological and molecular characterization of 100 samples of L. monocytogenes isolated from human (43) and food (57) sources, from several regions of Brazil, and collected between 1975 and 2013. Antigenic characterization defined 49% of serotype 4b samples, followed by 28% of serotype 1/2b, 14% of serotype 1/2c, 8% of serotype 1/2a, and 1% of serotype 3b. Both type of samples from human and food origin express the same serotype distribution. Multiplex PCR analysis showed 13 strains of type 4b with the amplification profile 4b-VI (Variant I). Virulence genes hly, inlA, inlB, inlC, inlJ, actA, plcA, and prfA were detected in all samples, highlighting a deletion of 105pb on the actA gene in 23% of serotype 4b samples. Macrorestriction profile with ApaI at PFGE showed 55 pulsotypes, with the occurrence of the same pulsotype in hospitalized patients in São Paulo in 1992 and 1997, and two other highly related pulsotypes in patients hospitalized in Rio de Janeiro in 2008. Recognized pulsotypes in listeriosis cases have also been detected in food. Thus, the prevalence of a serotype and the persistence of certain pulsotypes herald future problems.Brazilian Society of Infectious Diseases2017-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000300282Brazilian Journal of Infectious Diseases v.21 n.3 2017reponame:Brazilian Journal of Infectious Diseasesinstname:Brazilian Society of Infectious Diseases (BSID)instacron:BSID10.1016/j.bjid.2017.01.004info:eu-repo/semantics/openAccessAlmeida,Rosana Macedo deBarbosa,André VictorLisbôa,Rodrigo de CastroSantos,André Felipe das MercêsHofer,ErnestoVallim,Deyse ChristinaHofer,Cristina Barrosoeng2017-06-19T00:00:00Zoai:scielo:S1413-86702017000300282Revistahttps://www.bjid.org.br/https://old.scielo.br/oai/scielo-oai.phpbjid@bjid.org.br||lgoldani@ufrgs.br1678-43911413-8670opendoar:2017-06-19T00:00Brazilian Journal of Infectious Diseases - Brazilian Society of Infectious Diseases (BSID)false
dc.title.none.fl_str_mv Virulence genes and genetic relationship of L. monocytogenes isolated from human and food sources in Brazil
title Virulence genes and genetic relationship of L. monocytogenes isolated from human and food sources in Brazil
spellingShingle Virulence genes and genetic relationship of L. monocytogenes isolated from human and food sources in Brazil
Almeida,Rosana Macedo de
Listeria monocytogenes
Listeriosis
Virulence
Food
PFGE
Brazil
title_short Virulence genes and genetic relationship of L. monocytogenes isolated from human and food sources in Brazil
title_full Virulence genes and genetic relationship of L. monocytogenes isolated from human and food sources in Brazil
title_fullStr Virulence genes and genetic relationship of L. monocytogenes isolated from human and food sources in Brazil
title_full_unstemmed Virulence genes and genetic relationship of L. monocytogenes isolated from human and food sources in Brazil
title_sort Virulence genes and genetic relationship of L. monocytogenes isolated from human and food sources in Brazil
author Almeida,Rosana Macedo de
author_facet Almeida,Rosana Macedo de
Barbosa,André Victor
Lisbôa,Rodrigo de Castro
Santos,André Felipe das Mercês
Hofer,Ernesto
Vallim,Deyse Christina
Hofer,Cristina Barroso
author_role author
author2 Barbosa,André Victor
Lisbôa,Rodrigo de Castro
Santos,André Felipe das Mercês
Hofer,Ernesto
Vallim,Deyse Christina
Hofer,Cristina Barroso
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Almeida,Rosana Macedo de
Barbosa,André Victor
Lisbôa,Rodrigo de Castro
Santos,André Felipe das Mercês
Hofer,Ernesto
Vallim,Deyse Christina
Hofer,Cristina Barroso
dc.subject.por.fl_str_mv Listeria monocytogenes
Listeriosis
Virulence
Food
PFGE
Brazil
topic Listeria monocytogenes
Listeriosis
Virulence
Food
PFGE
Brazil
description ABSTRACT The herein presented assay provided a bacteriological and molecular characterization of 100 samples of L. monocytogenes isolated from human (43) and food (57) sources, from several regions of Brazil, and collected between 1975 and 2013. Antigenic characterization defined 49% of serotype 4b samples, followed by 28% of serotype 1/2b, 14% of serotype 1/2c, 8% of serotype 1/2a, and 1% of serotype 3b. Both type of samples from human and food origin express the same serotype distribution. Multiplex PCR analysis showed 13 strains of type 4b with the amplification profile 4b-VI (Variant I). Virulence genes hly, inlA, inlB, inlC, inlJ, actA, plcA, and prfA were detected in all samples, highlighting a deletion of 105pb on the actA gene in 23% of serotype 4b samples. Macrorestriction profile with ApaI at PFGE showed 55 pulsotypes, with the occurrence of the same pulsotype in hospitalized patients in São Paulo in 1992 and 1997, and two other highly related pulsotypes in patients hospitalized in Rio de Janeiro in 2008. Recognized pulsotypes in listeriosis cases have also been detected in food. Thus, the prevalence of a serotype and the persistence of certain pulsotypes herald future problems.
publishDate 2017
dc.date.none.fl_str_mv 2017-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000300282
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000300282
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1016/j.bjid.2017.01.004
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Brazilian Society of Infectious Diseases
publisher.none.fl_str_mv Brazilian Society of Infectious Diseases
dc.source.none.fl_str_mv Brazilian Journal of Infectious Diseases v.21 n.3 2017
reponame:Brazilian Journal of Infectious Diseases
instname:Brazilian Society of Infectious Diseases (BSID)
instacron:BSID
instname_str Brazilian Society of Infectious Diseases (BSID)
instacron_str BSID
institution BSID
reponame_str Brazilian Journal of Infectious Diseases
collection Brazilian Journal of Infectious Diseases
repository.name.fl_str_mv Brazilian Journal of Infectious Diseases - Brazilian Society of Infectious Diseases (BSID)
repository.mail.fl_str_mv bjid@bjid.org.br||lgoldani@ufrgs.br
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