Detection of HCV by PCR in serum and PBMC of patients with hepatitis C after treatment

Detalhes bibliográficos
Autor(a) principal: Cavalheiro,Norma de Paula
Data de Publicação: 2007
Outros Autores: Filgueiras,Thelma Cristina, Melo,Carlos Eduardo, Morimitsu,Suzana Rie, Araújo,Evaldo Stanislau Affonso de, Tengan,Fátima Mitiko, Barone,Antonio Alci
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Infectious Diseases
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000500006
Resumo: Although hepatitis C is mainly hepatotropic, some studies suggest that hepatitis C virus (HCV) infects peripheral blood mononuclear cells (PBMC), using them as a reservoir, which might contribute to the development of resistance to treatment. Fifty-four hepatitis-C patients, who had been submitted to treatment, were selected. Blood samples were collected on the same day for the detection of HCV RNA in serum and PBMC by PCR, using the Amplicor HCV 2.0 assay (Roche Diagnostics). HCV genotyping was performed using the INNO-LiPA HCV kit (Versant, Bayer Diagnostics). HCV RNA was detected in both serum and PBMC in 35 (64%) patients and no RNA in 16 (29.6%). Disagreement between the serum and PBMC results was observed for three patients (5.6%), with HCV RNA being detected in PBMC but not in serum. Four months later, new serum and PBMC samples were collected from one of these patients and HCV RNA was detected in both samples, showing that PBMC can reveal signs of a lack of response to treatment. We conclude that the absence of HCV in the serum of patients with chronic hepatitis C by the end of treatment does not mean that there is no circulating virus. HCV in mononuclear cells may be an indicator of the persisting infection.
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spelling Detection of HCV by PCR in serum and PBMC of patients with hepatitis C after treatmentPCRhepatitis CPBMCtreatmentAlthough hepatitis C is mainly hepatotropic, some studies suggest that hepatitis C virus (HCV) infects peripheral blood mononuclear cells (PBMC), using them as a reservoir, which might contribute to the development of resistance to treatment. Fifty-four hepatitis-C patients, who had been submitted to treatment, were selected. Blood samples were collected on the same day for the detection of HCV RNA in serum and PBMC by PCR, using the Amplicor HCV 2.0 assay (Roche Diagnostics). HCV genotyping was performed using the INNO-LiPA HCV kit (Versant, Bayer Diagnostics). HCV RNA was detected in both serum and PBMC in 35 (64%) patients and no RNA in 16 (29.6%). Disagreement between the serum and PBMC results was observed for three patients (5.6%), with HCV RNA being detected in PBMC but not in serum. Four months later, new serum and PBMC samples were collected from one of these patients and HCV RNA was detected in both samples, showing that PBMC can reveal signs of a lack of response to treatment. We conclude that the absence of HCV in the serum of patients with chronic hepatitis C by the end of treatment does not mean that there is no circulating virus. HCV in mononuclear cells may be an indicator of the persisting infection.Brazilian Society of Infectious Diseases2007-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000500006Brazilian Journal of Infectious Diseases v.11 n.5 2007reponame:Brazilian Journal of Infectious Diseasesinstname:Brazilian Society of Infectious Diseases (BSID)instacron:BSID10.1590/S1413-86702007000500006info:eu-repo/semantics/openAccessCavalheiro,Norma de PaulaFilgueiras,Thelma CristinaMelo,Carlos EduardoMorimitsu,Suzana RieAraújo,Evaldo Stanislau Affonso deTengan,Fátima MitikoBarone,Antonio Alcieng2007-10-22T00:00:00Zoai:scielo:S1413-86702007000500006Revistahttps://www.bjid.org.br/https://old.scielo.br/oai/scielo-oai.phpbjid@bjid.org.br||lgoldani@ufrgs.br1678-43911413-8670opendoar:2007-10-22T00:00Brazilian Journal of Infectious Diseases - Brazilian Society of Infectious Diseases (BSID)false
dc.title.none.fl_str_mv Detection of HCV by PCR in serum and PBMC of patients with hepatitis C after treatment
title Detection of HCV by PCR in serum and PBMC of patients with hepatitis C after treatment
spellingShingle Detection of HCV by PCR in serum and PBMC of patients with hepatitis C after treatment
Cavalheiro,Norma de Paula
PCR
hepatitis C
PBMC
treatment
title_short Detection of HCV by PCR in serum and PBMC of patients with hepatitis C after treatment
title_full Detection of HCV by PCR in serum and PBMC of patients with hepatitis C after treatment
title_fullStr Detection of HCV by PCR in serum and PBMC of patients with hepatitis C after treatment
title_full_unstemmed Detection of HCV by PCR in serum and PBMC of patients with hepatitis C after treatment
title_sort Detection of HCV by PCR in serum and PBMC of patients with hepatitis C after treatment
author Cavalheiro,Norma de Paula
author_facet Cavalheiro,Norma de Paula
Filgueiras,Thelma Cristina
Melo,Carlos Eduardo
Morimitsu,Suzana Rie
Araújo,Evaldo Stanislau Affonso de
Tengan,Fátima Mitiko
Barone,Antonio Alci
author_role author
author2 Filgueiras,Thelma Cristina
Melo,Carlos Eduardo
Morimitsu,Suzana Rie
Araújo,Evaldo Stanislau Affonso de
Tengan,Fátima Mitiko
Barone,Antonio Alci
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Cavalheiro,Norma de Paula
Filgueiras,Thelma Cristina
Melo,Carlos Eduardo
Morimitsu,Suzana Rie
Araújo,Evaldo Stanislau Affonso de
Tengan,Fátima Mitiko
Barone,Antonio Alci
dc.subject.por.fl_str_mv PCR
hepatitis C
PBMC
treatment
topic PCR
hepatitis C
PBMC
treatment
description Although hepatitis C is mainly hepatotropic, some studies suggest that hepatitis C virus (HCV) infects peripheral blood mononuclear cells (PBMC), using them as a reservoir, which might contribute to the development of resistance to treatment. Fifty-four hepatitis-C patients, who had been submitted to treatment, were selected. Blood samples were collected on the same day for the detection of HCV RNA in serum and PBMC by PCR, using the Amplicor HCV 2.0 assay (Roche Diagnostics). HCV genotyping was performed using the INNO-LiPA HCV kit (Versant, Bayer Diagnostics). HCV RNA was detected in both serum and PBMC in 35 (64%) patients and no RNA in 16 (29.6%). Disagreement between the serum and PBMC results was observed for three patients (5.6%), with HCV RNA being detected in PBMC but not in serum. Four months later, new serum and PBMC samples were collected from one of these patients and HCV RNA was detected in both samples, showing that PBMC can reveal signs of a lack of response to treatment. We conclude that the absence of HCV in the serum of patients with chronic hepatitis C by the end of treatment does not mean that there is no circulating virus. HCV in mononuclear cells may be an indicator of the persisting infection.
publishDate 2007
dc.date.none.fl_str_mv 2007-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000500006
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000500006
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1413-86702007000500006
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Brazilian Society of Infectious Diseases
publisher.none.fl_str_mv Brazilian Society of Infectious Diseases
dc.source.none.fl_str_mv Brazilian Journal of Infectious Diseases v.11 n.5 2007
reponame:Brazilian Journal of Infectious Diseases
instname:Brazilian Society of Infectious Diseases (BSID)
instacron:BSID
instname_str Brazilian Society of Infectious Diseases (BSID)
instacron_str BSID
institution BSID
reponame_str Brazilian Journal of Infectious Diseases
collection Brazilian Journal of Infectious Diseases
repository.name.fl_str_mv Brazilian Journal of Infectious Diseases - Brazilian Society of Infectious Diseases (BSID)
repository.mail.fl_str_mv bjid@bjid.org.br||lgoldani@ufrgs.br
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