The accessory gene regulator (agr) controls Staphylococcus aureus virulence in a murine intracranial abscesses model
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Infectious Diseases |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000500501 |
Resumo: | Background:Intracranial abscesses are associated with high mortality. Staphylococcus aureus is one of the main pathogens that cause intracranial infection. Until now, there is no report to identify the key effectors of S. aureus during the intracranial infection.Methods:The murine intracranial abscesses model induced by S. aureuswas constructed. The vital sign and survival rate of mice were observed to evaluate the infection. Histological examination was used to diagnose the pathological alterations of mouse tissues. The sensitivity of S. aureus to whole blood was evaluated by whole-blood killing assay.Results:In murine intracranial abscesses model, it was shown that the mortality caused by the accessory gene regulator (agr) locus deficient strain was significant decreased compared with its parent strain. Moreover, we found that RNAIII, the effector of agr system, was essential for the intracranial infection caused by S. aureus. In the further investigation, it was shown that restoration the expression of α-toxin in agr deficient strain could partially recover the mortality in the murine intracranial abscesses model.Conclusion:Our data suggested that the agr system of S. aureus is an important virulence determinant in the induction and mortality of intracranial abscesses in mice. |
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Brazilian Journal of Infectious Diseases |
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The accessory gene regulator (agr) controls Staphylococcus aureus virulence in a murine intracranial abscesses modelS. aureusAgrIntracranial abscesses modelBackground:Intracranial abscesses are associated with high mortality. Staphylococcus aureus is one of the main pathogens that cause intracranial infection. Until now, there is no report to identify the key effectors of S. aureus during the intracranial infection.Methods:The murine intracranial abscesses model induced by S. aureuswas constructed. The vital sign and survival rate of mice were observed to evaluate the infection. Histological examination was used to diagnose the pathological alterations of mouse tissues. The sensitivity of S. aureus to whole blood was evaluated by whole-blood killing assay.Results:In murine intracranial abscesses model, it was shown that the mortality caused by the accessory gene regulator (agr) locus deficient strain was significant decreased compared with its parent strain. Moreover, we found that RNAIII, the effector of agr system, was essential for the intracranial infection caused by S. aureus. In the further investigation, it was shown that restoration the expression of α-toxin in agr deficient strain could partially recover the mortality in the murine intracranial abscesses model.Conclusion:Our data suggested that the agr system of S. aureus is an important virulence determinant in the induction and mortality of intracranial abscesses in mice.Brazilian Society of Infectious Diseases2014-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000500501Brazilian Journal of Infectious Diseases v.18 n.5 2014reponame:Brazilian Journal of Infectious Diseasesinstname:Brazilian Society of Infectious Diseases (BSID)instacron:BSID10.1016/j.bjid.2014.03.005info:eu-repo/semantics/openAccessGong,JianLi,DongzhiYan,JunLiu,YuLi,DiDong,JieGao,YapingSun,TaoYang,Guangeng2015-09-01T00:00:00Zoai:scielo:S1413-86702014000500501Revistahttps://www.bjid.org.br/https://old.scielo.br/oai/scielo-oai.phpbjid@bjid.org.br||lgoldani@ufrgs.br1678-43911413-8670opendoar:2015-09-01T00:00Brazilian Journal of Infectious Diseases - Brazilian Society of Infectious Diseases (BSID)false |
dc.title.none.fl_str_mv |
The accessory gene regulator (agr) controls Staphylococcus aureus virulence in a murine intracranial abscesses model |
title |
The accessory gene regulator (agr) controls Staphylococcus aureus virulence in a murine intracranial abscesses model |
spellingShingle |
The accessory gene regulator (agr) controls Staphylococcus aureus virulence in a murine intracranial abscesses model Gong,Jian S. aureus Agr Intracranial abscesses model |
title_short |
The accessory gene regulator (agr) controls Staphylococcus aureus virulence in a murine intracranial abscesses model |
title_full |
The accessory gene regulator (agr) controls Staphylococcus aureus virulence in a murine intracranial abscesses model |
title_fullStr |
The accessory gene regulator (agr) controls Staphylococcus aureus virulence in a murine intracranial abscesses model |
title_full_unstemmed |
The accessory gene regulator (agr) controls Staphylococcus aureus virulence in a murine intracranial abscesses model |
title_sort |
The accessory gene regulator (agr) controls Staphylococcus aureus virulence in a murine intracranial abscesses model |
author |
Gong,Jian |
author_facet |
Gong,Jian Li,Dongzhi Yan,Jun Liu,Yu Li,Di Dong,Jie Gao,Yaping Sun,Tao Yang,Guang |
author_role |
author |
author2 |
Li,Dongzhi Yan,Jun Liu,Yu Li,Di Dong,Jie Gao,Yaping Sun,Tao Yang,Guang |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Gong,Jian Li,Dongzhi Yan,Jun Liu,Yu Li,Di Dong,Jie Gao,Yaping Sun,Tao Yang,Guang |
dc.subject.por.fl_str_mv |
S. aureus Agr Intracranial abscesses model |
topic |
S. aureus Agr Intracranial abscesses model |
description |
Background:Intracranial abscesses are associated with high mortality. Staphylococcus aureus is one of the main pathogens that cause intracranial infection. Until now, there is no report to identify the key effectors of S. aureus during the intracranial infection.Methods:The murine intracranial abscesses model induced by S. aureuswas constructed. The vital sign and survival rate of mice were observed to evaluate the infection. Histological examination was used to diagnose the pathological alterations of mouse tissues. The sensitivity of S. aureus to whole blood was evaluated by whole-blood killing assay.Results:In murine intracranial abscesses model, it was shown that the mortality caused by the accessory gene regulator (agr) locus deficient strain was significant decreased compared with its parent strain. Moreover, we found that RNAIII, the effector of agr system, was essential for the intracranial infection caused by S. aureus. In the further investigation, it was shown that restoration the expression of α-toxin in agr deficient strain could partially recover the mortality in the murine intracranial abscesses model.Conclusion:Our data suggested that the agr system of S. aureus is an important virulence determinant in the induction and mortality of intracranial abscesses in mice. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-10-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000500501 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000500501 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1016/j.bjid.2014.03.005 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Brazilian Society of Infectious Diseases |
publisher.none.fl_str_mv |
Brazilian Society of Infectious Diseases |
dc.source.none.fl_str_mv |
Brazilian Journal of Infectious Diseases v.18 n.5 2014 reponame:Brazilian Journal of Infectious Diseases instname:Brazilian Society of Infectious Diseases (BSID) instacron:BSID |
instname_str |
Brazilian Society of Infectious Diseases (BSID) |
instacron_str |
BSID |
institution |
BSID |
reponame_str |
Brazilian Journal of Infectious Diseases |
collection |
Brazilian Journal of Infectious Diseases |
repository.name.fl_str_mv |
Brazilian Journal of Infectious Diseases - Brazilian Society of Infectious Diseases (BSID) |
repository.mail.fl_str_mv |
bjid@bjid.org.br||lgoldani@ufrgs.br |
_version_ |
1754209243192360960 |