Use of the D test method to detect inducible clindamycin resistance in coagulase negative staphylococci (CoNS)

• Autor(a) principal: Perez,Leandro Reus Rodrigues
• Data de Publicação: 2007
• Outros Autores: Caierão,Juliana, Antunes,Ana Lúcia Souza, d'Azevedo,Pedro Alves
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Brazilian Journal of Infectious Diseases
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000200002
• topic: Clindamycin; resistance; D test

According to the National Committee for Clinical Laboratory Standards (NCCLS, 2004), a method to evaluate the inducible clindamycin resistance in accordance with an approach of the disks of erythromycin and clindamycin - the D test - has been reported. We analyzed the performance of this method in 200 coagulase negative staphylococci (CoNS) strains obtained from blood cultures of hospitalized patients at a general hospital in Southern Brazil. Twenty-seven clinical isolates with suitable profile (erythromycin-resistant and clindamycin-susceptible) were evaluated for the D test realization. Thus, only 5 CoNS were D test positive. The D test method showed to be simple and an important technique in the detection of inducible clindamycin resistance.