Effect of amikacin, cephalothin, clindamycin and vancomycin on in vitro fibroblast growth

Detalhes bibliográficos
Autor(a) principal: Souza,Fernanda Timm Seabra
Data de Publicação: 2004
Outros Autores: Mello,Alexandre Silva de, Michelin,Kristiane, Coelho,Janice Carneiro
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000300023
Resumo: The effect of four antibiotics (amikacin, clindamycin, cephalothin and vancomycin) was investigated considering that bacterial infection in fibroblasts cultures is a very frequent event. The investigation included the effect of the antibiotics on fibroblast growth and on the activity of the enzyme glucocerebrosidase. The antibiotics were added to the fibroblast cultures and cell growth was evaluated by counting the number of cells and their viability. After cell harvesting, the enzyme activity and content of protein were measured. The results allowed us to conclude that none of the antibiotics affected the cellular number nor the cellular viability. The content of protein decreased when cephalothin and clindamycin were added to the cultures, and glucocerebrosidase was affected in the presence of amikacin. Vancomycin did not interfere with any of the parameters analyzed, so it was chosen to be used in cell cultures to prevent the contamination by gram positive bacteria.
id SBG-1_8779ebf027d4dec6cbcde6e091607f33
oai_identifier_str oai:scielo:S1415-47572004000300023
network_acronym_str SBG-1
network_name_str Genetics and Molecular Biology
repository_id_str
spelling Effect of amikacin, cephalothin, clindamycin and vancomycin on in vitro fibroblast growthamikacincephalothinclindamycinvancomycinfibroblastscell cultureThe effect of four antibiotics (amikacin, clindamycin, cephalothin and vancomycin) was investigated considering that bacterial infection in fibroblasts cultures is a very frequent event. The investigation included the effect of the antibiotics on fibroblast growth and on the activity of the enzyme glucocerebrosidase. The antibiotics were added to the fibroblast cultures and cell growth was evaluated by counting the number of cells and their viability. After cell harvesting, the enzyme activity and content of protein were measured. The results allowed us to conclude that none of the antibiotics affected the cellular number nor the cellular viability. The content of protein decreased when cephalothin and clindamycin were added to the cultures, and glucocerebrosidase was affected in the presence of amikacin. Vancomycin did not interfere with any of the parameters analyzed, so it was chosen to be used in cell cultures to prevent the contamination by gram positive bacteria.Sociedade Brasileira de Genética2004-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000300023Genetics and Molecular Biology v.27 n.3 2004reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572004000300023info:eu-repo/semantics/openAccessSouza,Fernanda Timm SeabraMello,Alexandre Silva deMichelin,KristianeCoelho,Janice Carneiroeng2004-09-01T00:00:00Zoai:scielo:S1415-47572004000300023Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2004-09-01T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv Effect of amikacin, cephalothin, clindamycin and vancomycin on in vitro fibroblast growth
title Effect of amikacin, cephalothin, clindamycin and vancomycin on in vitro fibroblast growth
spellingShingle Effect of amikacin, cephalothin, clindamycin and vancomycin on in vitro fibroblast growth
Souza,Fernanda Timm Seabra
amikacin
cephalothin
clindamycin
vancomycin
fibroblasts
cell culture
title_short Effect of amikacin, cephalothin, clindamycin and vancomycin on in vitro fibroblast growth
title_full Effect of amikacin, cephalothin, clindamycin and vancomycin on in vitro fibroblast growth
title_fullStr Effect of amikacin, cephalothin, clindamycin and vancomycin on in vitro fibroblast growth
title_full_unstemmed Effect of amikacin, cephalothin, clindamycin and vancomycin on in vitro fibroblast growth
title_sort Effect of amikacin, cephalothin, clindamycin and vancomycin on in vitro fibroblast growth
author Souza,Fernanda Timm Seabra
author_facet Souza,Fernanda Timm Seabra
Mello,Alexandre Silva de
Michelin,Kristiane
Coelho,Janice Carneiro
author_role author
author2 Mello,Alexandre Silva de
Michelin,Kristiane
Coelho,Janice Carneiro
author2_role author
author
author
dc.contributor.author.fl_str_mv Souza,Fernanda Timm Seabra
Mello,Alexandre Silva de
Michelin,Kristiane
Coelho,Janice Carneiro
dc.subject.por.fl_str_mv amikacin
cephalothin
clindamycin
vancomycin
fibroblasts
cell culture
topic amikacin
cephalothin
clindamycin
vancomycin
fibroblasts
cell culture
description The effect of four antibiotics (amikacin, clindamycin, cephalothin and vancomycin) was investigated considering that bacterial infection in fibroblasts cultures is a very frequent event. The investigation included the effect of the antibiotics on fibroblast growth and on the activity of the enzyme glucocerebrosidase. The antibiotics were added to the fibroblast cultures and cell growth was evaluated by counting the number of cells and their viability. After cell harvesting, the enzyme activity and content of protein were measured. The results allowed us to conclude that none of the antibiotics affected the cellular number nor the cellular viability. The content of protein decreased when cephalothin and clindamycin were added to the cultures, and glucocerebrosidase was affected in the presence of amikacin. Vancomycin did not interfere with any of the parameters analyzed, so it was chosen to be used in cell cultures to prevent the contamination by gram positive bacteria.
publishDate 2004
dc.date.none.fl_str_mv 2004-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000300023
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000300023
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1415-47572004000300023
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.27 n.3 2004
reponame:Genetics and Molecular Biology
instname:Sociedade Brasileira de Genética (SBG)
instacron:SBG
instname_str Sociedade Brasileira de Genética (SBG)
instacron_str SBG
institution SBG
reponame_str Genetics and Molecular Biology
collection Genetics and Molecular Biology
repository.name.fl_str_mv Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)
repository.mail.fl_str_mv ||editor@gmb.org.br
_version_ 1752122379076632576

Registros relacionados