Causal variant loci and protein-coding genes for soybean bacterial pustule resistance in the flowering stage
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Crop Breeding and Applied Biotechnology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332022000100205 |
Resumo: | Abstract Bacterial pustule is an important soybean disease caused by Xanthomonas citri pv. glycines, but information about genetic resistance to this pathogen is scarce. This study aimed to investigate soybean genetic resistance to bacterial pustule in the flowering stage through association analysis, characterization of candidate SNP markers, and identification of protein-coding genes potentially regulating defense processes. Therefore, 109 soybean cultivars genotyped with a 6k Illumina platform were assessed for disease severity. A genome-wide analysis revealed a total of 13 SNPs significantly associated. Through protein annotation, we identified three markers located inside the coding regions of uncharacterized protein LOC100779077, histone-lysine N-methyltransferase SUVR4, and ABC-transporter B family member-9. Nucleotide polymorphism on the first two of these markers produces a non-synonymous polymorphism with polarity shift from hydrophobic to polar amino acid. It is convenient to prioritize these three candidate markers for validation procedures with the purpose of using them in marker-assisted soybean breeding programs. |
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Crop Breeding and Applied Biotechnology |
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Causal variant loci and protein-coding genes for soybean bacterial pustule resistance in the flowering stageBacterial pustuleGlycine max (L.) MerriGWASSNPcandidate genesAbstract Bacterial pustule is an important soybean disease caused by Xanthomonas citri pv. glycines, but information about genetic resistance to this pathogen is scarce. This study aimed to investigate soybean genetic resistance to bacterial pustule in the flowering stage through association analysis, characterization of candidate SNP markers, and identification of protein-coding genes potentially regulating defense processes. Therefore, 109 soybean cultivars genotyped with a 6k Illumina platform were assessed for disease severity. A genome-wide analysis revealed a total of 13 SNPs significantly associated. Through protein annotation, we identified three markers located inside the coding regions of uncharacterized protein LOC100779077, histone-lysine N-methyltransferase SUVR4, and ABC-transporter B family member-9. Nucleotide polymorphism on the first two of these markers produces a non-synonymous polymorphism with polarity shift from hydrophobic to polar amino acid. It is convenient to prioritize these three candidate markers for validation procedures with the purpose of using them in marker-assisted soybean breeding programs.Crop Breeding and Applied Biotechnology2022-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332022000100205Crop Breeding and Applied Biotechnology v.22 n.1 2022reponame:Crop Breeding and Applied Biotechnologyinstname:Sociedade Brasileira de Melhoramento de Plantasinstacron:CBAB10.1590/1984-70332022v22n1a07info:eu-repo/semantics/openAccessFonseca,Pollyanna Capobiango daFerreira,Dalton de Oliveira de OliveiraMorgan,TúlioMendes,Tiago Antônio de Oliveira eSilva,Felipe Lopes daeng2022-05-02T00:00:00Zoai:scielo:S1984-70332022000100205Revistahttps://cbab.sbmp.org.br/#ONGhttps://old.scielo.br/oai/scielo-oai.phpcbabjournal@gmail.com||cbab@ufv.br1984-70331518-7853opendoar:2022-05-02T00:00Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantasfalse |
dc.title.none.fl_str_mv |
Causal variant loci and protein-coding genes for soybean bacterial pustule resistance in the flowering stage |
title |
Causal variant loci and protein-coding genes for soybean bacterial pustule resistance in the flowering stage |
spellingShingle |
Causal variant loci and protein-coding genes for soybean bacterial pustule resistance in the flowering stage Fonseca,Pollyanna Capobiango da Bacterial pustule Glycine max (L.) Merri GWAS SNP candidate genes |
title_short |
Causal variant loci and protein-coding genes for soybean bacterial pustule resistance in the flowering stage |
title_full |
Causal variant loci and protein-coding genes for soybean bacterial pustule resistance in the flowering stage |
title_fullStr |
Causal variant loci and protein-coding genes for soybean bacterial pustule resistance in the flowering stage |
title_full_unstemmed |
Causal variant loci and protein-coding genes for soybean bacterial pustule resistance in the flowering stage |
title_sort |
Causal variant loci and protein-coding genes for soybean bacterial pustule resistance in the flowering stage |
author |
Fonseca,Pollyanna Capobiango da |
author_facet |
Fonseca,Pollyanna Capobiango da Ferreira,Dalton de Oliveira de Oliveira Morgan,Túlio Mendes,Tiago Antônio de Oliveira e Silva,Felipe Lopes da |
author_role |
author |
author2 |
Ferreira,Dalton de Oliveira de Oliveira Morgan,Túlio Mendes,Tiago Antônio de Oliveira e Silva,Felipe Lopes da |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Fonseca,Pollyanna Capobiango da Ferreira,Dalton de Oliveira de Oliveira Morgan,Túlio Mendes,Tiago Antônio de Oliveira e Silva,Felipe Lopes da |
dc.subject.por.fl_str_mv |
Bacterial pustule Glycine max (L.) Merri GWAS SNP candidate genes |
topic |
Bacterial pustule Glycine max (L.) Merri GWAS SNP candidate genes |
description |
Abstract Bacterial pustule is an important soybean disease caused by Xanthomonas citri pv. glycines, but information about genetic resistance to this pathogen is scarce. This study aimed to investigate soybean genetic resistance to bacterial pustule in the flowering stage through association analysis, characterization of candidate SNP markers, and identification of protein-coding genes potentially regulating defense processes. Therefore, 109 soybean cultivars genotyped with a 6k Illumina platform were assessed for disease severity. A genome-wide analysis revealed a total of 13 SNPs significantly associated. Through protein annotation, we identified three markers located inside the coding regions of uncharacterized protein LOC100779077, histone-lysine N-methyltransferase SUVR4, and ABC-transporter B family member-9. Nucleotide polymorphism on the first two of these markers produces a non-synonymous polymorphism with polarity shift from hydrophobic to polar amino acid. It is convenient to prioritize these three candidate markers for validation procedures with the purpose of using them in marker-assisted soybean breeding programs. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332022000100205 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332022000100205 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1984-70332022v22n1a07 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Crop Breeding and Applied Biotechnology |
publisher.none.fl_str_mv |
Crop Breeding and Applied Biotechnology |
dc.source.none.fl_str_mv |
Crop Breeding and Applied Biotechnology v.22 n.1 2022 reponame:Crop Breeding and Applied Biotechnology instname:Sociedade Brasileira de Melhoramento de Plantas instacron:CBAB |
instname_str |
Sociedade Brasileira de Melhoramento de Plantas |
instacron_str |
CBAB |
institution |
CBAB |
reponame_str |
Crop Breeding and Applied Biotechnology |
collection |
Crop Breeding and Applied Biotechnology |
repository.name.fl_str_mv |
Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantas |
repository.mail.fl_str_mv |
cbabjournal@gmail.com||cbab@ufv.br |
_version_ |
1754209188478713856 |