Breeding for PVY resistance in tobacco LJ911 using CRISPR/Cas9 technology

Detalhes bibliográficos
Autor(a) principal: Ruyi,Ren
Data de Publicação: 2021
Outros Autores: Qiang,Zheng, Futai,Ni, Qiu,Jin, Xiuqing,Wan, Jicheng,Wei
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Crop Breeding and Applied Biotechnology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332021000100206
Resumo: Abstract Potato virus Y (PVY) is one of the main diseases of tobacco (Nicotiana tabacum). Recent studies indicated that the Va gene (Ntab0942120) determines the susceptibility of the crop to PVY, and that the Va gene product interacts with the PVY genome-linked protein (VPg) to initiate the PVY genome translation process, which ultimately leads to the systemic infection of tobacco by the virus. In this research, tobacco cultivar LJ911 was used as receptor material for gene editing. Gene Va was knocked out through CRISPR/Cas9 technology, and transgene-free homozygous edited plants in the T1 generation were established. Pathology tests indicated that the edited plants had gained PVY resistance. Therefore, the edited materials generated in this study represent potentially useful genetic resources for breeding of PVY- resistant tobacco.
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spelling Breeding for PVY resistance in tobacco LJ911 using CRISPR/Cas9 technologyPVYtobaccoCRISPR/Cas9Varesistance breedingAbstract Potato virus Y (PVY) is one of the main diseases of tobacco (Nicotiana tabacum). Recent studies indicated that the Va gene (Ntab0942120) determines the susceptibility of the crop to PVY, and that the Va gene product interacts with the PVY genome-linked protein (VPg) to initiate the PVY genome translation process, which ultimately leads to the systemic infection of tobacco by the virus. In this research, tobacco cultivar LJ911 was used as receptor material for gene editing. Gene Va was knocked out through CRISPR/Cas9 technology, and transgene-free homozygous edited plants in the T1 generation were established. Pathology tests indicated that the edited plants had gained PVY resistance. Therefore, the edited materials generated in this study represent potentially useful genetic resources for breeding of PVY- resistant tobacco.Crop Breeding and Applied Biotechnology2021-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332021000100206Crop Breeding and Applied Biotechnology v.21 n.1 2021reponame:Crop Breeding and Applied Biotechnologyinstname:Sociedade Brasileira de Melhoramento de Plantasinstacron:CBAB10.1590/1984-70332021v21n1a6info:eu-repo/semantics/openAccessRuyi,RenQiang,ZhengFutai,NiQiu,JinXiuqing,WanJicheng,Weieng2021-04-07T00:00:00Zoai:scielo:S1984-70332021000100206Revistahttps://cbab.sbmp.org.br/#ONGhttps://old.scielo.br/oai/scielo-oai.phpcbabjournal@gmail.com||cbab@ufv.br1984-70331518-7853opendoar:2021-04-07T00:00Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantasfalse
dc.title.none.fl_str_mv Breeding for PVY resistance in tobacco LJ911 using CRISPR/Cas9 technology
title Breeding for PVY resistance in tobacco LJ911 using CRISPR/Cas9 technology
spellingShingle Breeding for PVY resistance in tobacco LJ911 using CRISPR/Cas9 technology
Ruyi,Ren
PVY
tobacco
CRISPR/Cas9
Va
resistance breeding
title_short Breeding for PVY resistance in tobacco LJ911 using CRISPR/Cas9 technology
title_full Breeding for PVY resistance in tobacco LJ911 using CRISPR/Cas9 technology
title_fullStr Breeding for PVY resistance in tobacco LJ911 using CRISPR/Cas9 technology
title_full_unstemmed Breeding for PVY resistance in tobacco LJ911 using CRISPR/Cas9 technology
title_sort Breeding for PVY resistance in tobacco LJ911 using CRISPR/Cas9 technology
author Ruyi,Ren
author_facet Ruyi,Ren
Qiang,Zheng
Futai,Ni
Qiu,Jin
Xiuqing,Wan
Jicheng,Wei
author_role author
author2 Qiang,Zheng
Futai,Ni
Qiu,Jin
Xiuqing,Wan
Jicheng,Wei
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Ruyi,Ren
Qiang,Zheng
Futai,Ni
Qiu,Jin
Xiuqing,Wan
Jicheng,Wei
dc.subject.por.fl_str_mv PVY
tobacco
CRISPR/Cas9
Va
resistance breeding
topic PVY
tobacco
CRISPR/Cas9
Va
resistance breeding
description Abstract Potato virus Y (PVY) is one of the main diseases of tobacco (Nicotiana tabacum). Recent studies indicated that the Va gene (Ntab0942120) determines the susceptibility of the crop to PVY, and that the Va gene product interacts with the PVY genome-linked protein (VPg) to initiate the PVY genome translation process, which ultimately leads to the systemic infection of tobacco by the virus. In this research, tobacco cultivar LJ911 was used as receptor material for gene editing. Gene Va was knocked out through CRISPR/Cas9 technology, and transgene-free homozygous edited plants in the T1 generation were established. Pathology tests indicated that the edited plants had gained PVY resistance. Therefore, the edited materials generated in this study represent potentially useful genetic resources for breeding of PVY- resistant tobacco.
publishDate 2021
dc.date.none.fl_str_mv 2021-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332021000100206
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332021000100206
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1984-70332021v21n1a6
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Crop Breeding and Applied Biotechnology
publisher.none.fl_str_mv Crop Breeding and Applied Biotechnology
dc.source.none.fl_str_mv Crop Breeding and Applied Biotechnology v.21 n.1 2021
reponame:Crop Breeding and Applied Biotechnology
instname:Sociedade Brasileira de Melhoramento de Plantas
instacron:CBAB
instname_str Sociedade Brasileira de Melhoramento de Plantas
instacron_str CBAB
institution CBAB
reponame_str Crop Breeding and Applied Biotechnology
collection Crop Breeding and Applied Biotechnology
repository.name.fl_str_mv Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantas
repository.mail.fl_str_mv cbabjournal@gmail.com||cbab@ufv.br
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