A multiplex PCR system for detection of wide compatibility allele S5-n and erect panicle allele dep1 in rice

Detalhes bibliográficos
Autor(a) principal: Li,Jun Zhou
Data de Publicação: 2017
Outros Autores: Cao,Yong Run, Li,Meng Qi, Zhao,Wen Li, Sun,Hong Zheng, Zhao,Quan Zhi
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Crop Breeding and Applied Biotechnology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332017000300250
Resumo: Abstract Wide compatibility and erect panicles are important traits for heterosis and ideal plant type breeding in rice and accordingly are targets for rice yield improvement. In this study, a multiplex PCR system based on functional markers was developed to simultaneously identify genotypes of the wide compatibility allele S5-n and the erect panicle allele dep1. Forty-nine rice varieties in the Huang-Huai-Hai region of China were identified using this system. Thirty-three varieties contained the erect panicle allele dep1 and two varieties contained the wide compatibility allele S5-n. The frequency of dep1 was obviously higher than that of S5-n. The multiplex PCR method was also tested using an F2 segregating population and was found to be simple, efficient, and reliable. It will be useful in marker-assisted selection based on the wide compatibility allele S5-n and erect panicle allele dep1 to breed super rice varieties using indica and japonica heterosis.
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spelling A multiplex PCR system for detection of wide compatibility allele S5-n and erect panicle allele dep1 in riceFunctional markerS5-nDEP1multiplex PCRAbstract Wide compatibility and erect panicles are important traits for heterosis and ideal plant type breeding in rice and accordingly are targets for rice yield improvement. In this study, a multiplex PCR system based on functional markers was developed to simultaneously identify genotypes of the wide compatibility allele S5-n and the erect panicle allele dep1. Forty-nine rice varieties in the Huang-Huai-Hai region of China were identified using this system. Thirty-three varieties contained the erect panicle allele dep1 and two varieties contained the wide compatibility allele S5-n. The frequency of dep1 was obviously higher than that of S5-n. The multiplex PCR method was also tested using an F2 segregating population and was found to be simple, efficient, and reliable. It will be useful in marker-assisted selection based on the wide compatibility allele S5-n and erect panicle allele dep1 to breed super rice varieties using indica and japonica heterosis.Crop Breeding and Applied Biotechnology2017-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332017000300250Crop Breeding and Applied Biotechnology v.17 n.3 2017reponame:Crop Breeding and Applied Biotechnologyinstname:Sociedade Brasileira de Melhoramento de Plantasinstacron:CBAB10.1590/1984-70332017v17n3a38info:eu-repo/semantics/openAccessLi,Jun ZhouCao,Yong RunLi,Meng QiZhao,Wen LiSun,Hong ZhengZhao,Quan Zhieng2017-07-21T00:00:00Zoai:scielo:S1984-70332017000300250Revistahttps://cbab.sbmp.org.br/#ONGhttps://old.scielo.br/oai/scielo-oai.phpcbabjournal@gmail.com||cbab@ufv.br1984-70331518-7853opendoar:2017-07-21T00:00Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantasfalse
dc.title.none.fl_str_mv A multiplex PCR system for detection of wide compatibility allele S5-n and erect panicle allele dep1 in rice
title A multiplex PCR system for detection of wide compatibility allele S5-n and erect panicle allele dep1 in rice
spellingShingle A multiplex PCR system for detection of wide compatibility allele S5-n and erect panicle allele dep1 in rice
Li,Jun Zhou
Functional marker
S5-n
DEP1
multiplex PCR
title_short A multiplex PCR system for detection of wide compatibility allele S5-n and erect panicle allele dep1 in rice
title_full A multiplex PCR system for detection of wide compatibility allele S5-n and erect panicle allele dep1 in rice
title_fullStr A multiplex PCR system for detection of wide compatibility allele S5-n and erect panicle allele dep1 in rice
title_full_unstemmed A multiplex PCR system for detection of wide compatibility allele S5-n and erect panicle allele dep1 in rice
title_sort A multiplex PCR system for detection of wide compatibility allele S5-n and erect panicle allele dep1 in rice
author Li,Jun Zhou
author_facet Li,Jun Zhou
Cao,Yong Run
Li,Meng Qi
Zhao,Wen Li
Sun,Hong Zheng
Zhao,Quan Zhi
author_role author
author2 Cao,Yong Run
Li,Meng Qi
Zhao,Wen Li
Sun,Hong Zheng
Zhao,Quan Zhi
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Li,Jun Zhou
Cao,Yong Run
Li,Meng Qi
Zhao,Wen Li
Sun,Hong Zheng
Zhao,Quan Zhi
dc.subject.por.fl_str_mv Functional marker
S5-n
DEP1
multiplex PCR
topic Functional marker
S5-n
DEP1
multiplex PCR
description Abstract Wide compatibility and erect panicles are important traits for heterosis and ideal plant type breeding in rice and accordingly are targets for rice yield improvement. In this study, a multiplex PCR system based on functional markers was developed to simultaneously identify genotypes of the wide compatibility allele S5-n and the erect panicle allele dep1. Forty-nine rice varieties in the Huang-Huai-Hai region of China were identified using this system. Thirty-three varieties contained the erect panicle allele dep1 and two varieties contained the wide compatibility allele S5-n. The frequency of dep1 was obviously higher than that of S5-n. The multiplex PCR method was also tested using an F2 segregating population and was found to be simple, efficient, and reliable. It will be useful in marker-assisted selection based on the wide compatibility allele S5-n and erect panicle allele dep1 to breed super rice varieties using indica and japonica heterosis.
publishDate 2017
dc.date.none.fl_str_mv 2017-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332017000300250
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332017000300250
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1984-70332017v17n3a38
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Crop Breeding and Applied Biotechnology
publisher.none.fl_str_mv Crop Breeding and Applied Biotechnology
dc.source.none.fl_str_mv Crop Breeding and Applied Biotechnology v.17 n.3 2017
reponame:Crop Breeding and Applied Biotechnology
instname:Sociedade Brasileira de Melhoramento de Plantas
instacron:CBAB
instname_str Sociedade Brasileira de Melhoramento de Plantas
instacron_str CBAB
institution CBAB
reponame_str Crop Breeding and Applied Biotechnology
collection Crop Breeding and Applied Biotechnology
repository.name.fl_str_mv Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantas
repository.mail.fl_str_mv cbabjournal@gmail.com||cbab@ufv.br
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