Efficient and new method for Tectona grandis in vitro regeneration

Detalhes bibliográficos
Autor(a) principal: Tambarussi,Evandro V.
Data de Publicação: 2017
Outros Autores: Rogalski,Marcelo, Galeano,Esteban, Brondani,Gilvano Ebling, Martin,Valentina de Fatima de, Silva,Lucas Américo da, Carrer,Helaine
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Crop Breeding and Applied Biotechnology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332017000200124
Resumo: Abstract The aim of this study was to develop a shoot regeneration protocol for Tectona grandis by examining the effects of pretreatments, regeneration culture medium, adventitious rooting, and acclimatization. The best organogenic potential was achieved when hypocotyls and cotyledonary segments from seedlings germinated on a medium containing TDZ, were cultured for four weeks on MS medium supplemented with BAP/GA3. With TDZ pretreatment during seed germination, up to 70% of the cotyledonary segments (0.5 TDZ pretreatment and 1.0 BAP + 0.5 GA3 during regeneration, mg L-1) and 60% of the hypocotyl explants (0.1 TDZ pretreatment and 1.0 BAP + 0.5 GA3 during regeneration, mg L-1) resulted in shoot regeneration. Finally, 65% of the regenerated shoots rooted, of which 60% were successfully acclimatized ex vitro. Our protocol increases regeneration efficiency for teak and it can serve as a platform for genetic transformation to improve this important tree species.
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spelling Efficient and new method for Tectona grandis in vitro regenerationAdventitious rootingplant growth regulatorplant tissue culturewoody plantAbstract The aim of this study was to develop a shoot regeneration protocol for Tectona grandis by examining the effects of pretreatments, regeneration culture medium, adventitious rooting, and acclimatization. The best organogenic potential was achieved when hypocotyls and cotyledonary segments from seedlings germinated on a medium containing TDZ, were cultured for four weeks on MS medium supplemented with BAP/GA3. With TDZ pretreatment during seed germination, up to 70% of the cotyledonary segments (0.5 TDZ pretreatment and 1.0 BAP + 0.5 GA3 during regeneration, mg L-1) and 60% of the hypocotyl explants (0.1 TDZ pretreatment and 1.0 BAP + 0.5 GA3 during regeneration, mg L-1) resulted in shoot regeneration. Finally, 65% of the regenerated shoots rooted, of which 60% were successfully acclimatized ex vitro. Our protocol increases regeneration efficiency for teak and it can serve as a platform for genetic transformation to improve this important tree species.Crop Breeding and Applied Biotechnology2017-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332017000200124Crop Breeding and Applied Biotechnology v.17 n.2 2017reponame:Crop Breeding and Applied Biotechnologyinstname:Sociedade Brasileira de Melhoramento de Plantasinstacron:CBAB10.1590/1984-70332017v17n2a19info:eu-repo/semantics/openAccessTambarussi,Evandro V.Rogalski,MarceloGaleano,EstebanBrondani,Gilvano EblingMartin,Valentina de Fatima deSilva,Lucas Américo daCarrer,Helaineeng2017-03-28T00:00:00Zoai:scielo:S1984-70332017000200124Revistahttps://cbab.sbmp.org.br/#ONGhttps://old.scielo.br/oai/scielo-oai.phpcbabjournal@gmail.com||cbab@ufv.br1984-70331518-7853opendoar:2017-03-28T00:00Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantasfalse
dc.title.none.fl_str_mv Efficient and new method for Tectona grandis in vitro regeneration
title Efficient and new method for Tectona grandis in vitro regeneration
spellingShingle Efficient and new method for Tectona grandis in vitro regeneration
Tambarussi,Evandro V.
Adventitious rooting
plant growth regulator
plant tissue culture
woody plant
title_short Efficient and new method for Tectona grandis in vitro regeneration
title_full Efficient and new method for Tectona grandis in vitro regeneration
title_fullStr Efficient and new method for Tectona grandis in vitro regeneration
title_full_unstemmed Efficient and new method for Tectona grandis in vitro regeneration
title_sort Efficient and new method for Tectona grandis in vitro regeneration
author Tambarussi,Evandro V.
author_facet Tambarussi,Evandro V.
Rogalski,Marcelo
Galeano,Esteban
Brondani,Gilvano Ebling
Martin,Valentina de Fatima de
Silva,Lucas Américo da
Carrer,Helaine
author_role author
author2 Rogalski,Marcelo
Galeano,Esteban
Brondani,Gilvano Ebling
Martin,Valentina de Fatima de
Silva,Lucas Américo da
Carrer,Helaine
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Tambarussi,Evandro V.
Rogalski,Marcelo
Galeano,Esteban
Brondani,Gilvano Ebling
Martin,Valentina de Fatima de
Silva,Lucas Américo da
Carrer,Helaine
dc.subject.por.fl_str_mv Adventitious rooting
plant growth regulator
plant tissue culture
woody plant
topic Adventitious rooting
plant growth regulator
plant tissue culture
woody plant
description Abstract The aim of this study was to develop a shoot regeneration protocol for Tectona grandis by examining the effects of pretreatments, regeneration culture medium, adventitious rooting, and acclimatization. The best organogenic potential was achieved when hypocotyls and cotyledonary segments from seedlings germinated on a medium containing TDZ, were cultured for four weeks on MS medium supplemented with BAP/GA3. With TDZ pretreatment during seed germination, up to 70% of the cotyledonary segments (0.5 TDZ pretreatment and 1.0 BAP + 0.5 GA3 during regeneration, mg L-1) and 60% of the hypocotyl explants (0.1 TDZ pretreatment and 1.0 BAP + 0.5 GA3 during regeneration, mg L-1) resulted in shoot regeneration. Finally, 65% of the regenerated shoots rooted, of which 60% were successfully acclimatized ex vitro. Our protocol increases regeneration efficiency for teak and it can serve as a platform for genetic transformation to improve this important tree species.
publishDate 2017
dc.date.none.fl_str_mv 2017-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332017000200124
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332017000200124
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1984-70332017v17n2a19
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Crop Breeding and Applied Biotechnology
publisher.none.fl_str_mv Crop Breeding and Applied Biotechnology
dc.source.none.fl_str_mv Crop Breeding and Applied Biotechnology v.17 n.2 2017
reponame:Crop Breeding and Applied Biotechnology
instname:Sociedade Brasileira de Melhoramento de Plantas
instacron:CBAB
instname_str Sociedade Brasileira de Melhoramento de Plantas
instacron_str CBAB
institution CBAB
reponame_str Crop Breeding and Applied Biotechnology
collection Crop Breeding and Applied Biotechnology
repository.name.fl_str_mv Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantas
repository.mail.fl_str_mv cbabjournal@gmail.com||cbab@ufv.br
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