Efficient and new method for Tectona grandis in vitro regeneration
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | http://dx.doi.org/10.1590/1984-70332017v17n2a19 http://www.locus.ufv.br/handle/123456789/18171 |
Resumo: | O artigo não contém resumo em português. |
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LOCUS Repositório Institucional da UFV |
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Rogalski, MarceloTambarussi, Evandro V.Galeano, EstebanBrondani, Gilvano EblingMartin, Valentina de Fatima deSilva, Lucas Américo daCarrer, Helaine2018-03-12T10:51:29Z2018-03-12T10:51:29Z2017-03-1719847033http://dx.doi.org/10.1590/1984-70332017v17n2a19http://www.locus.ufv.br/handle/123456789/18171O artigo não contém resumo em português.The aim of this study was to develop a shoot regeneration protocol for Tectona grandis by examining the effects of pretreatments, regeneration culture medium, adventitious rooting, and acclimatization. The best organogenic potential was achieved when hypocotyls and cotyledonary segments from seedlings germinated on a medium containing TDZ, were cultured for four weeks on MS medium supplemented with BAP/GA3. With TDZ pretreatment during seed germination, up to 70% of the cotyledonary segments (0.5 TDZ pretreatment and 1.0 BAP + 0.5 GA3 during regeneration, mg L-1) and 60% of the hypocotyl explants (0.1 TDZ pretreatment and 1.0 BAP + 0.5 GA3 during regeneration, mg L-1) resulted in shoot regeneration. Finally, 65% of the regenerated shoots rooted, of which 60% were successfully acclimatized ex vitro. Our protocol increases regeneration efficiency for teak and it can serve as a platform for genetic transformation to improve this important tree species.engCrop Breeding and Applied Biotechnologyv. 17, n. 2, p. 124-132, Junho 2017Adventitious rootingPlant growth regulatorPlant tissue cultureWoody plantEfficient and new method for Tectona grandis in vitro regenerationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALartigo.pdfartigo.pdftexto completoapplication/pdf736888https://locus.ufv.br//bitstream/123456789/18171/1/artigo.pdf0ff371a1c9eff3690f7f9c9062c0e2b3MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/18171/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52THUMBNAILartigo.pdf.jpgartigo.pdf.jpgIM Thumbnailimage/jpeg4714https://locus.ufv.br//bitstream/123456789/18171/3/artigo.pdf.jpg373767039ef21a10d402a481dbf49457MD53123456789/181712018-03-12 23:00:35.932oai:locus.ufv.br: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Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452018-03-13T02:00:35LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.en.fl_str_mv |
Efficient and new method for Tectona grandis in vitro regeneration |
title |
Efficient and new method for Tectona grandis in vitro regeneration |
spellingShingle |
Efficient and new method for Tectona grandis in vitro regeneration Rogalski, Marcelo Adventitious rooting Plant growth regulator Plant tissue culture Woody plant |
title_short |
Efficient and new method for Tectona grandis in vitro regeneration |
title_full |
Efficient and new method for Tectona grandis in vitro regeneration |
title_fullStr |
Efficient and new method for Tectona grandis in vitro regeneration |
title_full_unstemmed |
Efficient and new method for Tectona grandis in vitro regeneration |
title_sort |
Efficient and new method for Tectona grandis in vitro regeneration |
author |
Rogalski, Marcelo |
author_facet |
Rogalski, Marcelo Tambarussi, Evandro V. Galeano, Esteban Brondani, Gilvano Ebling Martin, Valentina de Fatima de Silva, Lucas Américo da Carrer, Helaine |
author_role |
author |
author2 |
Tambarussi, Evandro V. Galeano, Esteban Brondani, Gilvano Ebling Martin, Valentina de Fatima de Silva, Lucas Américo da Carrer, Helaine |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Rogalski, Marcelo Tambarussi, Evandro V. Galeano, Esteban Brondani, Gilvano Ebling Martin, Valentina de Fatima de Silva, Lucas Américo da Carrer, Helaine |
dc.subject.pt-BR.fl_str_mv |
Adventitious rooting Plant growth regulator Plant tissue culture Woody plant |
topic |
Adventitious rooting Plant growth regulator Plant tissue culture Woody plant |
description |
O artigo não contém resumo em português. |
publishDate |
2017 |
dc.date.issued.fl_str_mv |
2017-03-17 |
dc.date.accessioned.fl_str_mv |
2018-03-12T10:51:29Z |
dc.date.available.fl_str_mv |
2018-03-12T10:51:29Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/1984-70332017v17n2a19 http://www.locus.ufv.br/handle/123456789/18171 |
dc.identifier.issn.none.fl_str_mv |
19847033 |
identifier_str_mv |
19847033 |
url |
http://dx.doi.org/10.1590/1984-70332017v17n2a19 http://www.locus.ufv.br/handle/123456789/18171 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartofseries.pt-BR.fl_str_mv |
v. 17, n. 2, p. 124-132, Junho 2017 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Crop Breeding and Applied Biotechnology |
publisher.none.fl_str_mv |
Crop Breeding and Applied Biotechnology |
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Universidade Federal de Viçosa (UFV) |
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LOCUS Repositório Institucional da UFV |
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LOCUS Repositório Institucional da UFV |
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