Selection of legitimate dwarf coconut hybrid seedlings using DNA fingerprinting
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Crop Breeding and Applied Biotechnology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332018000400409 |
Resumo: | Abstract Due to the great economic importance of coconut palm in Brazil and the development of a coconut breeding program intended to produce intravarietal hybrids, the present work aimed to ease the production of hybrids with the same morphological heritage. DNA was extracted from leaf samples of 13 dwarf coconut populations from Brazil, and PCR amplification was performed using 21 previously selected microsatellites. Furthermore, this study selected nine microsatellite markers with the potential to identify Green Dwarf x Yellow Dwarf hybrids and 16 microsatellites with the potential to identify Red Dwarf x Yellow Dwarf hybrids. In conclusion, SSR marker based on DNA Fingerprinting allowed the accurate identification of legitimate intravarietal hybrids since, for those crosses, the methodology of identification based on seedling color is not a viable alternative. |
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Crop Breeding and Applied Biotechnology |
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Selection of legitimate dwarf coconut hybrid seedlings using DNA fingerprintingCocos nucifera L.intravarietal hybridmicrosatellite MarkersAbstract Due to the great economic importance of coconut palm in Brazil and the development of a coconut breeding program intended to produce intravarietal hybrids, the present work aimed to ease the production of hybrids with the same morphological heritage. DNA was extracted from leaf samples of 13 dwarf coconut populations from Brazil, and PCR amplification was performed using 21 previously selected microsatellites. Furthermore, this study selected nine microsatellite markers with the potential to identify Green Dwarf x Yellow Dwarf hybrids and 16 microsatellites with the potential to identify Red Dwarf x Yellow Dwarf hybrids. In conclusion, SSR marker based on DNA Fingerprinting allowed the accurate identification of legitimate intravarietal hybrids since, for those crosses, the methodology of identification based on seedling color is not a viable alternative.Crop Breeding and Applied Biotechnology2018-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332018000400409Crop Breeding and Applied Biotechnology v.18 n.4 2018reponame:Crop Breeding and Applied Biotechnologyinstname:Sociedade Brasileira de Melhoramento de Plantasinstacron:CBAB10.1590/1984-70332018v18n4a60info:eu-repo/semantics/openAccessAzevedo,Alinne Oliveira NunesAzevedo,Carlos Diego de OliveiraSantos,Pedro Henrique Araújo DinizRamos,Helaine Christine CancelaBoechat,Marcela Santana BastosArêdes,Fernanda Abreu SantanaRamos,Semíramis Rabelo RamalhoMirizola,Luís ÂngeloPerera,LalithAragão,Wilson MenesesPereira,Messias Gonzagaeng2018-12-03T00:00:00Zoai:scielo:S1984-70332018000400409Revistahttps://cbab.sbmp.org.br/#ONGhttps://old.scielo.br/oai/scielo-oai.phpcbabjournal@gmail.com||cbab@ufv.br1984-70331518-7853opendoar:2018-12-03T00:00Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantasfalse |
dc.title.none.fl_str_mv |
Selection of legitimate dwarf coconut hybrid seedlings using DNA fingerprinting |
title |
Selection of legitimate dwarf coconut hybrid seedlings using DNA fingerprinting |
spellingShingle |
Selection of legitimate dwarf coconut hybrid seedlings using DNA fingerprinting Azevedo,Alinne Oliveira Nunes Cocos nucifera L. intravarietal hybrid microsatellite Markers |
title_short |
Selection of legitimate dwarf coconut hybrid seedlings using DNA fingerprinting |
title_full |
Selection of legitimate dwarf coconut hybrid seedlings using DNA fingerprinting |
title_fullStr |
Selection of legitimate dwarf coconut hybrid seedlings using DNA fingerprinting |
title_full_unstemmed |
Selection of legitimate dwarf coconut hybrid seedlings using DNA fingerprinting |
title_sort |
Selection of legitimate dwarf coconut hybrid seedlings using DNA fingerprinting |
author |
Azevedo,Alinne Oliveira Nunes |
author_facet |
Azevedo,Alinne Oliveira Nunes Azevedo,Carlos Diego de Oliveira Santos,Pedro Henrique Araújo Diniz Ramos,Helaine Christine Cancela Boechat,Marcela Santana Bastos Arêdes,Fernanda Abreu Santana Ramos,Semíramis Rabelo Ramalho Mirizola,Luís Ângelo Perera,Lalith Aragão,Wilson Meneses Pereira,Messias Gonzaga |
author_role |
author |
author2 |
Azevedo,Carlos Diego de Oliveira Santos,Pedro Henrique Araújo Diniz Ramos,Helaine Christine Cancela Boechat,Marcela Santana Bastos Arêdes,Fernanda Abreu Santana Ramos,Semíramis Rabelo Ramalho Mirizola,Luís Ângelo Perera,Lalith Aragão,Wilson Meneses Pereira,Messias Gonzaga |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Azevedo,Alinne Oliveira Nunes Azevedo,Carlos Diego de Oliveira Santos,Pedro Henrique Araújo Diniz Ramos,Helaine Christine Cancela Boechat,Marcela Santana Bastos Arêdes,Fernanda Abreu Santana Ramos,Semíramis Rabelo Ramalho Mirizola,Luís Ângelo Perera,Lalith Aragão,Wilson Meneses Pereira,Messias Gonzaga |
dc.subject.por.fl_str_mv |
Cocos nucifera L. intravarietal hybrid microsatellite Markers |
topic |
Cocos nucifera L. intravarietal hybrid microsatellite Markers |
description |
Abstract Due to the great economic importance of coconut palm in Brazil and the development of a coconut breeding program intended to produce intravarietal hybrids, the present work aimed to ease the production of hybrids with the same morphological heritage. DNA was extracted from leaf samples of 13 dwarf coconut populations from Brazil, and PCR amplification was performed using 21 previously selected microsatellites. Furthermore, this study selected nine microsatellite markers with the potential to identify Green Dwarf x Yellow Dwarf hybrids and 16 microsatellites with the potential to identify Red Dwarf x Yellow Dwarf hybrids. In conclusion, SSR marker based on DNA Fingerprinting allowed the accurate identification of legitimate intravarietal hybrids since, for those crosses, the methodology of identification based on seedling color is not a viable alternative. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332018000400409 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332018000400409 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1984-70332018v18n4a60 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Crop Breeding and Applied Biotechnology |
publisher.none.fl_str_mv |
Crop Breeding and Applied Biotechnology |
dc.source.none.fl_str_mv |
Crop Breeding and Applied Biotechnology v.18 n.4 2018 reponame:Crop Breeding and Applied Biotechnology instname:Sociedade Brasileira de Melhoramento de Plantas instacron:CBAB |
instname_str |
Sociedade Brasileira de Melhoramento de Plantas |
instacron_str |
CBAB |
institution |
CBAB |
reponame_str |
Crop Breeding and Applied Biotechnology |
collection |
Crop Breeding and Applied Biotechnology |
repository.name.fl_str_mv |
Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantas |
repository.mail.fl_str_mv |
cbabjournal@gmail.com||cbab@ufv.br |
_version_ |
1754209187613638656 |