Effects of immersion system and gibberellic acid on the growth and acclimatization of micropropagated pineapple

Detalhes bibliográficos
Autor(a) principal: Scherer,Ramon Felipe
Data de Publicação: 2015
Outros Autores: Holderbaum,Daniel Ferreira, Garcia,Antônio Corrrêa, Silva,Dorival Almeida da, Steinmacher,Douglas André, Guerra,Miguel Pedro
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Crop Breeding and Applied Biotechnology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332015000200066
Resumo: Micropropagation based techniques enable the mass production of pest- and disease-free stock plants. Aiming at optimizing pineapple [Ananas comosus (L.) Merril] micropropagation, a complete randomized experiment with three factors was established: immersion system (2 L twin flasks temporary immersion and permanent immersion); culture medium (supplemented or not with GA3); and shoot height class. The effects on regeneration rate (number of new shoots per 1 g fresh mass (FM) of inoculated microshoots), FM per shoot, and shoot survival were evaluated. Significant interactions (p<0.05) were observed in the regeneration rate, and simple effects (p<0.05) were detected for FM per shoot. All treatments resulted in a survival rate higher than 95%. Altogether, it was found that 2 L twin flasks temporary immersion and MS culture medium free of GA3 allowed the optimization of the protocol used. This treatment was tested with positive response in a biofactory system that uses sterile plastic bottles (5 L).
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spelling Effects of immersion system and gibberellic acid on the growth and acclimatization of micropropagated pineappleMicropropagationregeneration ratebiofactoryMicropropagation based techniques enable the mass production of pest- and disease-free stock plants. Aiming at optimizing pineapple [Ananas comosus (L.) Merril] micropropagation, a complete randomized experiment with three factors was established: immersion system (2 L twin flasks temporary immersion and permanent immersion); culture medium (supplemented or not with GA3); and shoot height class. The effects on regeneration rate (number of new shoots per 1 g fresh mass (FM) of inoculated microshoots), FM per shoot, and shoot survival were evaluated. Significant interactions (p<0.05) were observed in the regeneration rate, and simple effects (p<0.05) were detected for FM per shoot. All treatments resulted in a survival rate higher than 95%. Altogether, it was found that 2 L twin flasks temporary immersion and MS culture medium free of GA3 allowed the optimization of the protocol used. This treatment was tested with positive response in a biofactory system that uses sterile plastic bottles (5 L). Crop Breeding and Applied Biotechnology2015-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332015000200066Crop Breeding and Applied Biotechnology v.15 n.2 2015reponame:Crop Breeding and Applied Biotechnologyinstname:Sociedade Brasileira de Melhoramento de Plantasinstacron:CBAB10.1590/1984-70332015v15n2a13info:eu-repo/semantics/openAccessScherer,Ramon FelipeHolderbaum,Daniel FerreiraGarcia,Antônio CorrrêaSilva,Dorival Almeida daSteinmacher,Douglas AndréGuerra,Miguel Pedroeng2015-05-15T00:00:00Zoai:scielo:S1984-70332015000200066Revistahttps://cbab.sbmp.org.br/#ONGhttps://old.scielo.br/oai/scielo-oai.phpcbabjournal@gmail.com||cbab@ufv.br1984-70331518-7853opendoar:2015-05-15T00:00Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantasfalse
dc.title.none.fl_str_mv Effects of immersion system and gibberellic acid on the growth and acclimatization of micropropagated pineapple
title Effects of immersion system and gibberellic acid on the growth and acclimatization of micropropagated pineapple
spellingShingle Effects of immersion system and gibberellic acid on the growth and acclimatization of micropropagated pineapple
Scherer,Ramon Felipe
Micropropagation
regeneration rate
biofactory
title_short Effects of immersion system and gibberellic acid on the growth and acclimatization of micropropagated pineapple
title_full Effects of immersion system and gibberellic acid on the growth and acclimatization of micropropagated pineapple
title_fullStr Effects of immersion system and gibberellic acid on the growth and acclimatization of micropropagated pineapple
title_full_unstemmed Effects of immersion system and gibberellic acid on the growth and acclimatization of micropropagated pineapple
title_sort Effects of immersion system and gibberellic acid on the growth and acclimatization of micropropagated pineapple
author Scherer,Ramon Felipe
author_facet Scherer,Ramon Felipe
Holderbaum,Daniel Ferreira
Garcia,Antônio Corrrêa
Silva,Dorival Almeida da
Steinmacher,Douglas André
Guerra,Miguel Pedro
author_role author
author2 Holderbaum,Daniel Ferreira
Garcia,Antônio Corrrêa
Silva,Dorival Almeida da
Steinmacher,Douglas André
Guerra,Miguel Pedro
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Scherer,Ramon Felipe
Holderbaum,Daniel Ferreira
Garcia,Antônio Corrrêa
Silva,Dorival Almeida da
Steinmacher,Douglas André
Guerra,Miguel Pedro
dc.subject.por.fl_str_mv Micropropagation
regeneration rate
biofactory
topic Micropropagation
regeneration rate
biofactory
description Micropropagation based techniques enable the mass production of pest- and disease-free stock plants. Aiming at optimizing pineapple [Ananas comosus (L.) Merril] micropropagation, a complete randomized experiment with three factors was established: immersion system (2 L twin flasks temporary immersion and permanent immersion); culture medium (supplemented or not with GA3); and shoot height class. The effects on regeneration rate (number of new shoots per 1 g fresh mass (FM) of inoculated microshoots), FM per shoot, and shoot survival were evaluated. Significant interactions (p<0.05) were observed in the regeneration rate, and simple effects (p<0.05) were detected for FM per shoot. All treatments resulted in a survival rate higher than 95%. Altogether, it was found that 2 L twin flasks temporary immersion and MS culture medium free of GA3 allowed the optimization of the protocol used. This treatment was tested with positive response in a biofactory system that uses sterile plastic bottles (5 L).
publishDate 2015
dc.date.none.fl_str_mv 2015-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332015000200066
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332015000200066
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1984-70332015v15n2a13
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Crop Breeding and Applied Biotechnology
publisher.none.fl_str_mv Crop Breeding and Applied Biotechnology
dc.source.none.fl_str_mv Crop Breeding and Applied Biotechnology v.15 n.2 2015
reponame:Crop Breeding and Applied Biotechnology
instname:Sociedade Brasileira de Melhoramento de Plantas
instacron:CBAB
instname_str Sociedade Brasileira de Melhoramento de Plantas
instacron_str CBAB
institution CBAB
reponame_str Crop Breeding and Applied Biotechnology
collection Crop Breeding and Applied Biotechnology
repository.name.fl_str_mv Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantas
repository.mail.fl_str_mv cbabjournal@gmail.com||cbab@ufv.br
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