Leaf anatomy of micropropagated pineapple plants

Detalhes bibliográficos
Autor(a) principal: Barboza, Sarah Brandão Santa Cruz
Data de Publicação: 2006
Outros Autores: Graciano-Ribeiro, Dalva, Teixeira, João Batista, Portes, Tomás Aquino, Souza, Luiz Augusto Copati
Tipo de documento: Artigo
Idioma: por
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/7118
Resumo: The objective of this work was to study leaf anatomy of pineapple plants in order to improve acclimatization protocols. In vitro plantlets weighting an average of 3.1 g and greenhouse plantlets derived from in vitro stock material, after six and ten months of culture, weighting an average of 50.2 and 65 g, respectively, were used. Stomatal density was determined on the abaxial epidermis, at the basal, median, and apical portions of the leaf, using a completely randomized design under 2x3 factorial (two culture environments and three leaf regions) with six replicates. Thickness of the hypodermis, aquiferous and photosynthetic parenchyma were determined at the median portion of the leaf using a totally randomized design with three treatments and four replicates. The basic structure of the pineapple leaf under in vitro conditions did not change. However, stomatal frequency, cuticular and epidermal wall thickening, shape and sinuosity of the cell walls of aquiferous parenchyma and the presence of papillary cells were verified as a result of environmental conditions during culture, indicating phenotypic plasticity.
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spelling Leaf anatomy of micropropagated pineapple plantsAnatomia foliar de plantas micropropagadas de abacaxiAnanas comosus; environment of cultivation; micropropagation; acclimatizationAnanas comosus; ambiente de cultivo; micropropagação; aclimataçãoThe objective of this work was to study leaf anatomy of pineapple plants in order to improve acclimatization protocols. In vitro plantlets weighting an average of 3.1 g and greenhouse plantlets derived from in vitro stock material, after six and ten months of culture, weighting an average of 50.2 and 65 g, respectively, were used. Stomatal density was determined on the abaxial epidermis, at the basal, median, and apical portions of the leaf, using a completely randomized design under 2x3 factorial (two culture environments and three leaf regions) with six replicates. Thickness of the hypodermis, aquiferous and photosynthetic parenchyma were determined at the median portion of the leaf using a totally randomized design with three treatments and four replicates. The basic structure of the pineapple leaf under in vitro conditions did not change. However, stomatal frequency, cuticular and epidermal wall thickening, shape and sinuosity of the cell walls of aquiferous parenchyma and the presence of papillary cells were verified as a result of environmental conditions during culture, indicating phenotypic plasticity.O objetivo deste trabalho foi indicar caracteres anatômicos em folhas de plantas micropropagadas de abacaxizeiro, visando ao aperfeiçoamento do protocolo de aclimatação. Utilizaram-se plântulas micropropagadas in vitro e aclimatadas por seis e dez meses, apresentando, em média, 3,1, 50,2 e 65 g, respectivamente. A densidade estomática foi determinada na face abaxial da epiderme, nas regiões basal, mediana e apical da folha, usando o delineamento inteiramente casualizado em esquema fatorial de 2x3 (dois ambientes de cultivo e três regiões da folha), em seis repetições. A espessura da hipoderme, parênquimas aqüífero e clorofilado foi determinada na região mediana da folha, usando-se o delineamento inteiramente casualizado com três tratamentos em quatro repetições. A estrutura básica da folha do abacaxizeiro não se modificou, entretanto, ocorreram diferenças na freqüência estomática, no espessamento da cutícula e paredes da epiderme, formato e sinuosidade das paredes das células do tecido aqüífero e presença de células papilosas nos diferentes ambientes de cultivo, indicando plasticidade fenotípica.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraBarboza, Sarah Brandão Santa CruzGraciano-Ribeiro, DalvaTeixeira, João BatistaPortes, Tomás AquinoSouza, Luiz Augusto Copati2006-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/7118Pesquisa Agropecuaria Brasileira; v.41, n.2, fev. 2006; 185-194Pesquisa Agropecuária Brasileira; v.41, n.2, fev. 2006; 185-1941678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/7118/4163info:eu-repo/semantics/openAccess2014-09-25T17:13:55Zoai:ojs.seer.sct.embrapa.br:article/7118Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-09-25T17:13:55Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Leaf anatomy of micropropagated pineapple plants
Anatomia foliar de plantas micropropagadas de abacaxi
title Leaf anatomy of micropropagated pineapple plants
spellingShingle Leaf anatomy of micropropagated pineapple plants
Barboza, Sarah Brandão Santa Cruz
Ananas comosus; environment of cultivation; micropropagation; acclimatization
Ananas comosus; ambiente de cultivo; micropropagação; aclimatação
title_short Leaf anatomy of micropropagated pineapple plants
title_full Leaf anatomy of micropropagated pineapple plants
title_fullStr Leaf anatomy of micropropagated pineapple plants
title_full_unstemmed Leaf anatomy of micropropagated pineapple plants
title_sort Leaf anatomy of micropropagated pineapple plants
author Barboza, Sarah Brandão Santa Cruz
author_facet Barboza, Sarah Brandão Santa Cruz
Graciano-Ribeiro, Dalva
Teixeira, João Batista
Portes, Tomás Aquino
Souza, Luiz Augusto Copati
author_role author
author2 Graciano-Ribeiro, Dalva
Teixeira, João Batista
Portes, Tomás Aquino
Souza, Luiz Augusto Copati
author2_role author
author
author
author
dc.contributor.none.fl_str_mv

dc.contributor.author.fl_str_mv Barboza, Sarah Brandão Santa Cruz
Graciano-Ribeiro, Dalva
Teixeira, João Batista
Portes, Tomás Aquino
Souza, Luiz Augusto Copati
dc.subject.por.fl_str_mv Ananas comosus; environment of cultivation; micropropagation; acclimatization
Ananas comosus; ambiente de cultivo; micropropagação; aclimatação
topic Ananas comosus; environment of cultivation; micropropagation; acclimatization
Ananas comosus; ambiente de cultivo; micropropagação; aclimatação
description The objective of this work was to study leaf anatomy of pineapple plants in order to improve acclimatization protocols. In vitro plantlets weighting an average of 3.1 g and greenhouse plantlets derived from in vitro stock material, after six and ten months of culture, weighting an average of 50.2 and 65 g, respectively, were used. Stomatal density was determined on the abaxial epidermis, at the basal, median, and apical portions of the leaf, using a completely randomized design under 2x3 factorial (two culture environments and three leaf regions) with six replicates. Thickness of the hypodermis, aquiferous and photosynthetic parenchyma were determined at the median portion of the leaf using a totally randomized design with three treatments and four replicates. The basic structure of the pineapple leaf under in vitro conditions did not change. However, stomatal frequency, cuticular and epidermal wall thickening, shape and sinuosity of the cell walls of aquiferous parenchyma and the presence of papillary cells were verified as a result of environmental conditions during culture, indicating phenotypic plasticity.
publishDate 2006
dc.date.none.fl_str_mv 2006-02-01
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/7118
url https://seer.sct.embrapa.br/index.php/pab/article/view/7118
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/7118/4163
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.41, n.2, fev. 2006; 185-194
Pesquisa Agropecuária Brasileira; v.41, n.2, fev. 2006; 185-194
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
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