In vitro multiplication of Eucalyptus hybrid via temporary immersion bioreactor: culture media and cytokinin effects

Detalhes bibliográficos
Autor(a) principal: Máximo,Wesley Pires Flausino
Data de Publicação: 2018
Outros Autores: Santos,Paulo Augusto Almeida, Martins,Guilherme Silva, Mendonça,Evânia Galvão, Paiva,Luciano Vilela
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Crop Breeding and Applied Biotechnology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332018000200131
Resumo: Abstract This study aimed to assess the influence of culture medium and cytokinin type on in vitro multiplication of an Eucalyptus grandis x E. urophylla hybrid clone via temporary immersion bioreactor (TIB®). JADS, modified MS at a (NO3ˉ):(NH +) ratio, and WPM were used as liquid media; and 6-benzilaminopurine (BAP), kinetin (KIN), and thidiazuron (TDZ) were the cytokinins used in the study. According to the results, explants under the influence of modified MS presented better results for all traits analyzed in this study. BAP was the most suitable plant growth regulator for axillary bud shoot proliferation. By the leaf histological analysis, most of the shoots grown in the presence of BAP either exhibited normal morphology or only a few hyperhydricity symptoms. All these results were obtained at 19 days after in vitro cultivation, a shorter period when compared with other works in the literature, showing the protocol efficiency in the multiplication of this hybrid clone using TIB®.
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spelling In vitro multiplication of Eucalyptus hybrid via temporary immersion bioreactor: culture media and cytokinin effects6-benzilaminopurinehyperhydricityplant biotechnologyin vitro propagationAbstract This study aimed to assess the influence of culture medium and cytokinin type on in vitro multiplication of an Eucalyptus grandis x E. urophylla hybrid clone via temporary immersion bioreactor (TIB®). JADS, modified MS at a (NO3ˉ):(NH +) ratio, and WPM were used as liquid media; and 6-benzilaminopurine (BAP), kinetin (KIN), and thidiazuron (TDZ) were the cytokinins used in the study. According to the results, explants under the influence of modified MS presented better results for all traits analyzed in this study. BAP was the most suitable plant growth regulator for axillary bud shoot proliferation. By the leaf histological analysis, most of the shoots grown in the presence of BAP either exhibited normal morphology or only a few hyperhydricity symptoms. All these results were obtained at 19 days after in vitro cultivation, a shorter period when compared with other works in the literature, showing the protocol efficiency in the multiplication of this hybrid clone using TIB®.Crop Breeding and Applied Biotechnology2018-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332018000200131Crop Breeding and Applied Biotechnology v.18 n.2 2018reponame:Crop Breeding and Applied Biotechnologyinstname:Sociedade Brasileira de Melhoramento de Plantasinstacron:CBAB10.1590/1984-70332018v18n2a19info:eu-repo/semantics/openAccessMáximo,Wesley Pires FlausinoSantos,Paulo Augusto AlmeidaMartins,Guilherme SilvaMendonça,Evânia GalvãoPaiva,Luciano Vilelaeng2018-04-25T00:00:00Zoai:scielo:S1984-70332018000200131Revistahttps://cbab.sbmp.org.br/#ONGhttps://old.scielo.br/oai/scielo-oai.phpcbabjournal@gmail.com||cbab@ufv.br1984-70331518-7853opendoar:2018-04-25T00:00Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantasfalse
dc.title.none.fl_str_mv In vitro multiplication of Eucalyptus hybrid via temporary immersion bioreactor: culture media and cytokinin effects
title In vitro multiplication of Eucalyptus hybrid via temporary immersion bioreactor: culture media and cytokinin effects
spellingShingle In vitro multiplication of Eucalyptus hybrid via temporary immersion bioreactor: culture media and cytokinin effects
Máximo,Wesley Pires Flausino
6-benzilaminopurine
hyperhydricity
plant biotechnology
in vitro propagation
title_short In vitro multiplication of Eucalyptus hybrid via temporary immersion bioreactor: culture media and cytokinin effects
title_full In vitro multiplication of Eucalyptus hybrid via temporary immersion bioreactor: culture media and cytokinin effects
title_fullStr In vitro multiplication of Eucalyptus hybrid via temporary immersion bioreactor: culture media and cytokinin effects
title_full_unstemmed In vitro multiplication of Eucalyptus hybrid via temporary immersion bioreactor: culture media and cytokinin effects
title_sort In vitro multiplication of Eucalyptus hybrid via temporary immersion bioreactor: culture media and cytokinin effects
author Máximo,Wesley Pires Flausino
author_facet Máximo,Wesley Pires Flausino
Santos,Paulo Augusto Almeida
Martins,Guilherme Silva
Mendonça,Evânia Galvão
Paiva,Luciano Vilela
author_role author
author2 Santos,Paulo Augusto Almeida
Martins,Guilherme Silva
Mendonça,Evânia Galvão
Paiva,Luciano Vilela
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Máximo,Wesley Pires Flausino
Santos,Paulo Augusto Almeida
Martins,Guilherme Silva
Mendonça,Evânia Galvão
Paiva,Luciano Vilela
dc.subject.por.fl_str_mv 6-benzilaminopurine
hyperhydricity
plant biotechnology
in vitro propagation
topic 6-benzilaminopurine
hyperhydricity
plant biotechnology
in vitro propagation
description Abstract This study aimed to assess the influence of culture medium and cytokinin type on in vitro multiplication of an Eucalyptus grandis x E. urophylla hybrid clone via temporary immersion bioreactor (TIB®). JADS, modified MS at a (NO3ˉ):(NH +) ratio, and WPM were used as liquid media; and 6-benzilaminopurine (BAP), kinetin (KIN), and thidiazuron (TDZ) were the cytokinins used in the study. According to the results, explants under the influence of modified MS presented better results for all traits analyzed in this study. BAP was the most suitable plant growth regulator for axillary bud shoot proliferation. By the leaf histological analysis, most of the shoots grown in the presence of BAP either exhibited normal morphology or only a few hyperhydricity symptoms. All these results were obtained at 19 days after in vitro cultivation, a shorter period when compared with other works in the literature, showing the protocol efficiency in the multiplication of this hybrid clone using TIB®.
publishDate 2018
dc.date.none.fl_str_mv 2018-04-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332018000200131
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332018000200131
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1984-70332018v18n2a19
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Crop Breeding and Applied Biotechnology
publisher.none.fl_str_mv Crop Breeding and Applied Biotechnology
dc.source.none.fl_str_mv Crop Breeding and Applied Biotechnology v.18 n.2 2018
reponame:Crop Breeding and Applied Biotechnology
instname:Sociedade Brasileira de Melhoramento de Plantas
instacron:CBAB
instname_str Sociedade Brasileira de Melhoramento de Plantas
instacron_str CBAB
institution CBAB
reponame_str Crop Breeding and Applied Biotechnology
collection Crop Breeding and Applied Biotechnology
repository.name.fl_str_mv Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantas
repository.mail.fl_str_mv cbabjournal@gmail.com||cbab@ufv.br
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