In vitro pollination and fluorescence microscopy for characterization of gametophytic self-incompatibility of Coffea canephora Pierre ex A. Froehner

Detalhes bibliográficos
Autor(a) principal: Souza,Carolina Augusto de
Data de Publicação: 2021
Outros Autores: Rocha,Rodrigo Barros, Santos,Maurício Reginaldo Alves dos, Lopes,Tatiane Almeida, Teixeira,Alexsandro Lara, Espindula,Marcelo Curitiba
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Crop Breeding and Applied Biotechnology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332021000400201
Resumo: Abstract The objectives of this study were to optimize in vitro pollination and fluorescence microscopy procedures to characterize the gametophytic self-incompatibility of Coffea canephora genotypes. For that purpose, 115 in vitro pollinations were carried out among 30 parent plants of the Conilon (17) and Robusta (13) botanical varieties. Tester plants of compatibility groups I, II, and III were used as pollen grain donors. On the day of anthesis, pollen grains of the donor genotypes were transferred in vitro to one of the sides of the bifid stigma of each receptor flower. After 30 hours, the development of the pollen tubes was evaluated in fluorescence microscopy. Eleven genotypes (37%) were classified in compatibility Group I, thirteen (43%) in Group II, and six (20%) in Group III. In vitro pollination allowed reduction in the time necessary to diagnose compatibility, without exhibiting contamination caused by the presence of pollen grains of non-donor plants.
id CBAB-1_da0b75ccfe1d661690c70a77d46b1f50
oai_identifier_str oai:scielo:S1984-70332021000400201
network_acronym_str CBAB-1
network_name_str Crop Breeding and Applied Biotechnology
repository_id_str
spelling In vitro pollination and fluorescence microscopy for characterization of gametophytic self-incompatibility of Coffea canephora Pierre ex A. FroehnerCanephora coffeeConilonRobustacompatibilityAbstract The objectives of this study were to optimize in vitro pollination and fluorescence microscopy procedures to characterize the gametophytic self-incompatibility of Coffea canephora genotypes. For that purpose, 115 in vitro pollinations were carried out among 30 parent plants of the Conilon (17) and Robusta (13) botanical varieties. Tester plants of compatibility groups I, II, and III were used as pollen grain donors. On the day of anthesis, pollen grains of the donor genotypes were transferred in vitro to one of the sides of the bifid stigma of each receptor flower. After 30 hours, the development of the pollen tubes was evaluated in fluorescence microscopy. Eleven genotypes (37%) were classified in compatibility Group I, thirteen (43%) in Group II, and six (20%) in Group III. In vitro pollination allowed reduction in the time necessary to diagnose compatibility, without exhibiting contamination caused by the presence of pollen grains of non-donor plants.Crop Breeding and Applied Biotechnology2021-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332021000400201Crop Breeding and Applied Biotechnology v.21 n.4 2021reponame:Crop Breeding and Applied Biotechnologyinstname:Sociedade Brasileira de Melhoramento de Plantasinstacron:CBAB10.1590/1984-70332021v21n4a51info:eu-repo/semantics/openAccessSouza,Carolina Augusto deRocha,Rodrigo BarrosSantos,Maurício Reginaldo Alves dosLopes,Tatiane AlmeidaTeixeira,Alexsandro LaraEspindula,Marcelo Curitibaeng2021-12-06T00:00:00Zoai:scielo:S1984-70332021000400201Revistahttps://cbab.sbmp.org.br/#ONGhttps://old.scielo.br/oai/scielo-oai.phpcbabjournal@gmail.com||cbab@ufv.br1984-70331518-7853opendoar:2021-12-06T00:00Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantasfalse
dc.title.none.fl_str_mv In vitro pollination and fluorescence microscopy for characterization of gametophytic self-incompatibility of Coffea canephora Pierre ex A. Froehner
title In vitro pollination and fluorescence microscopy for characterization of gametophytic self-incompatibility of Coffea canephora Pierre ex A. Froehner
spellingShingle In vitro pollination and fluorescence microscopy for characterization of gametophytic self-incompatibility of Coffea canephora Pierre ex A. Froehner
Souza,Carolina Augusto de
Canephora coffee
Conilon
Robusta
compatibility
title_short In vitro pollination and fluorescence microscopy for characterization of gametophytic self-incompatibility of Coffea canephora Pierre ex A. Froehner
title_full In vitro pollination and fluorescence microscopy for characterization of gametophytic self-incompatibility of Coffea canephora Pierre ex A. Froehner
title_fullStr In vitro pollination and fluorescence microscopy for characterization of gametophytic self-incompatibility of Coffea canephora Pierre ex A. Froehner
title_full_unstemmed In vitro pollination and fluorescence microscopy for characterization of gametophytic self-incompatibility of Coffea canephora Pierre ex A. Froehner
title_sort In vitro pollination and fluorescence microscopy for characterization of gametophytic self-incompatibility of Coffea canephora Pierre ex A. Froehner
author Souza,Carolina Augusto de
author_facet Souza,Carolina Augusto de
Rocha,Rodrigo Barros
Santos,Maurício Reginaldo Alves dos
Lopes,Tatiane Almeida
Teixeira,Alexsandro Lara
Espindula,Marcelo Curitiba
author_role author
author2 Rocha,Rodrigo Barros
Santos,Maurício Reginaldo Alves dos
Lopes,Tatiane Almeida
Teixeira,Alexsandro Lara
Espindula,Marcelo Curitiba
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Souza,Carolina Augusto de
Rocha,Rodrigo Barros
Santos,Maurício Reginaldo Alves dos
Lopes,Tatiane Almeida
Teixeira,Alexsandro Lara
Espindula,Marcelo Curitiba
dc.subject.por.fl_str_mv Canephora coffee
Conilon
Robusta
compatibility
topic Canephora coffee
Conilon
Robusta
compatibility
description Abstract The objectives of this study were to optimize in vitro pollination and fluorescence microscopy procedures to characterize the gametophytic self-incompatibility of Coffea canephora genotypes. For that purpose, 115 in vitro pollinations were carried out among 30 parent plants of the Conilon (17) and Robusta (13) botanical varieties. Tester plants of compatibility groups I, II, and III were used as pollen grain donors. On the day of anthesis, pollen grains of the donor genotypes were transferred in vitro to one of the sides of the bifid stigma of each receptor flower. After 30 hours, the development of the pollen tubes was evaluated in fluorescence microscopy. Eleven genotypes (37%) were classified in compatibility Group I, thirteen (43%) in Group II, and six (20%) in Group III. In vitro pollination allowed reduction in the time necessary to diagnose compatibility, without exhibiting contamination caused by the presence of pollen grains of non-donor plants.
publishDate 2021
dc.date.none.fl_str_mv 2021-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332021000400201
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332021000400201
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1984-70332021v21n4a51
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Crop Breeding and Applied Biotechnology
publisher.none.fl_str_mv Crop Breeding and Applied Biotechnology
dc.source.none.fl_str_mv Crop Breeding and Applied Biotechnology v.21 n.4 2021
reponame:Crop Breeding and Applied Biotechnology
instname:Sociedade Brasileira de Melhoramento de Plantas
instacron:CBAB
instname_str Sociedade Brasileira de Melhoramento de Plantas
instacron_str CBAB
institution CBAB
reponame_str Crop Breeding and Applied Biotechnology
collection Crop Breeding and Applied Biotechnology
repository.name.fl_str_mv Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantas
repository.mail.fl_str_mv cbabjournal@gmail.com||cbab@ufv.br
_version_ 1754209188429430784

Registros relacionados