Effects of dimethylsulfoxide and pentoxifylline in the vitality of cutaneous flaps in rats

Detalhes bibliográficos
Autor(a) principal: Pedretti,Stephanie Luzia da Costa
Data de Publicação: 2017
Outros Autores: Rena,Cícero de Lima, Castãnon,Maria Christina Marques Nogueira, Duque,Ana Paula do Nascimento, Pereira,Fernando Henrique, Nunes,Tarcizo Afonso
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista do Colégio Brasileiro de Cirurgiões
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-69912017000500457
Resumo: ABSTRACT Objectives: to verify the influence of dimethylsulfoxide and pentoxifylline on the vitality of cutaneous flaps in rats and the tissue repair process. Methods: were studied 30 Wistar rats, submitting them to a 2cm wide by 8cm long dorsal cutaneous flap, of caudal base. We distributed the animals in three groups: Control Group (n=10) with application gauze moistened with 0.9% Saline in the flap bed for 30 seconds; Dimethylsulfoxide group (n=10), with administration of 1ml of 5% dimethylsulfoxide divided into five injections of 0.2ml in the transition of the flap segments; Pentoxifylline group (n=10), with administration of pentoxifylline 20mg/kg, diluted to 1ml and divided into five injections of 0.2ml in the transition of the flap segments. Drugs were administered intraoperatively, in a single dose and subcutaneously. We observed the skin flaps for changes in color and texture. On the 10th postoperative day, we checked the dimensions of viable and necrotic tissues, followed by excision of the specimen for histological analysis. Results: the measurements of length of the viable and necrotic tissues between groups showed no differences. Histological analysis showed that the Dimethylsulfoxide group presented neovascularization, inflammatory infiltrate with leukocytes and more structured conjunctival stroma. The Pentoxifylline group showed neovascularization and inflammatory infiltrate, with moderate to intense granulation. The control group evolved with a higher rate of necrosis in the distal segment. Conclusion: dimethylsulfoxide and pentoxifylline influenced the vitality of the flap and the tissue repair process. However, they did not prevent necrosis macroscopically.
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spelling Effects of dimethylsulfoxide and pentoxifylline in the vitality of cutaneous flaps in ratsWound HealingDimethyl SulfoxidePentoxifyllineRatsSurgical flapsABSTRACT Objectives: to verify the influence of dimethylsulfoxide and pentoxifylline on the vitality of cutaneous flaps in rats and the tissue repair process. Methods: were studied 30 Wistar rats, submitting them to a 2cm wide by 8cm long dorsal cutaneous flap, of caudal base. We distributed the animals in three groups: Control Group (n=10) with application gauze moistened with 0.9% Saline in the flap bed for 30 seconds; Dimethylsulfoxide group (n=10), with administration of 1ml of 5% dimethylsulfoxide divided into five injections of 0.2ml in the transition of the flap segments; Pentoxifylline group (n=10), with administration of pentoxifylline 20mg/kg, diluted to 1ml and divided into five injections of 0.2ml in the transition of the flap segments. Drugs were administered intraoperatively, in a single dose and subcutaneously. We observed the skin flaps for changes in color and texture. On the 10th postoperative day, we checked the dimensions of viable and necrotic tissues, followed by excision of the specimen for histological analysis. Results: the measurements of length of the viable and necrotic tissues between groups showed no differences. Histological analysis showed that the Dimethylsulfoxide group presented neovascularization, inflammatory infiltrate with leukocytes and more structured conjunctival stroma. The Pentoxifylline group showed neovascularization and inflammatory infiltrate, with moderate to intense granulation. The control group evolved with a higher rate of necrosis in the distal segment. Conclusion: dimethylsulfoxide and pentoxifylline influenced the vitality of the flap and the tissue repair process. However, they did not prevent necrosis macroscopically.Colégio Brasileiro de Cirurgiões2017-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-69912017000500457Revista do Colégio Brasileiro de Cirurgiões v.44 n.5 2017reponame:Revista do Colégio Brasileiro de Cirurgiõesinstname:Colégio Brasileiro de Cirurgiões (CBC)instacron:CBC10.1590/0100-69912017005006info:eu-repo/semantics/openAccessPedretti,Stephanie Luzia da CostaRena,Cícero de LimaCastãnon,Maria Christina Marques NogueiraDuque,Ana Paula do NascimentoPereira,Fernando HenriqueNunes,Tarcizo Afonsoeng2018-03-08T00:00:00Zoai:scielo:S0100-69912017000500457Revistahttp://www.scielo.br/rcbcONGhttps://old.scielo.br/oai/scielo-oai.php||revistacbc@cbc.org.br1809-45460100-6991opendoar:2018-03-08T00:00Revista do Colégio Brasileiro de Cirurgiões - Colégio Brasileiro de Cirurgiões (CBC)false
dc.title.none.fl_str_mv Effects of dimethylsulfoxide and pentoxifylline in the vitality of cutaneous flaps in rats
title Effects of dimethylsulfoxide and pentoxifylline in the vitality of cutaneous flaps in rats
spellingShingle Effects of dimethylsulfoxide and pentoxifylline in the vitality of cutaneous flaps in rats
Pedretti,Stephanie Luzia da Costa
Wound Healing
Dimethyl Sulfoxide
Pentoxifylline
Rats
Surgical flaps
title_short Effects of dimethylsulfoxide and pentoxifylline in the vitality of cutaneous flaps in rats
title_full Effects of dimethylsulfoxide and pentoxifylline in the vitality of cutaneous flaps in rats
title_fullStr Effects of dimethylsulfoxide and pentoxifylline in the vitality of cutaneous flaps in rats
title_full_unstemmed Effects of dimethylsulfoxide and pentoxifylline in the vitality of cutaneous flaps in rats
title_sort Effects of dimethylsulfoxide and pentoxifylline in the vitality of cutaneous flaps in rats
author Pedretti,Stephanie Luzia da Costa
author_facet Pedretti,Stephanie Luzia da Costa
Rena,Cícero de Lima
Castãnon,Maria Christina Marques Nogueira
Duque,Ana Paula do Nascimento
Pereira,Fernando Henrique
Nunes,Tarcizo Afonso
author_role author
author2 Rena,Cícero de Lima
Castãnon,Maria Christina Marques Nogueira
Duque,Ana Paula do Nascimento
Pereira,Fernando Henrique
Nunes,Tarcizo Afonso
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Pedretti,Stephanie Luzia da Costa
Rena,Cícero de Lima
Castãnon,Maria Christina Marques Nogueira
Duque,Ana Paula do Nascimento
Pereira,Fernando Henrique
Nunes,Tarcizo Afonso
dc.subject.por.fl_str_mv Wound Healing
Dimethyl Sulfoxide
Pentoxifylline
Rats
Surgical flaps
topic Wound Healing
Dimethyl Sulfoxide
Pentoxifylline
Rats
Surgical flaps
description ABSTRACT Objectives: to verify the influence of dimethylsulfoxide and pentoxifylline on the vitality of cutaneous flaps in rats and the tissue repair process. Methods: were studied 30 Wistar rats, submitting them to a 2cm wide by 8cm long dorsal cutaneous flap, of caudal base. We distributed the animals in three groups: Control Group (n=10) with application gauze moistened with 0.9% Saline in the flap bed for 30 seconds; Dimethylsulfoxide group (n=10), with administration of 1ml of 5% dimethylsulfoxide divided into five injections of 0.2ml in the transition of the flap segments; Pentoxifylline group (n=10), with administration of pentoxifylline 20mg/kg, diluted to 1ml and divided into five injections of 0.2ml in the transition of the flap segments. Drugs were administered intraoperatively, in a single dose and subcutaneously. We observed the skin flaps for changes in color and texture. On the 10th postoperative day, we checked the dimensions of viable and necrotic tissues, followed by excision of the specimen for histological analysis. Results: the measurements of length of the viable and necrotic tissues between groups showed no differences. Histological analysis showed that the Dimethylsulfoxide group presented neovascularization, inflammatory infiltrate with leukocytes and more structured conjunctival stroma. The Pentoxifylline group showed neovascularization and inflammatory infiltrate, with moderate to intense granulation. The control group evolved with a higher rate of necrosis in the distal segment. Conclusion: dimethylsulfoxide and pentoxifylline influenced the vitality of the flap and the tissue repair process. However, they did not prevent necrosis macroscopically.
publishDate 2017
dc.date.none.fl_str_mv 2017-10-01
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dc.publisher.none.fl_str_mv Colégio Brasileiro de Cirurgiões
publisher.none.fl_str_mv Colégio Brasileiro de Cirurgiões
dc.source.none.fl_str_mv Revista do Colégio Brasileiro de Cirurgiões v.44 n.5 2017
reponame:Revista do Colégio Brasileiro de Cirurgiões
instname:Colégio Brasileiro de Cirurgiões (CBC)
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reponame_str Revista do Colégio Brasileiro de Cirurgiões
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repository.name.fl_str_mv Revista do Colégio Brasileiro de Cirurgiões - Colégio Brasileiro de Cirurgiões (CBC)
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