EFFECTS OF INTRAPERITONEAL GLUTAMINE IN THE TREATMENT OF EXPERIMENTAL SEPSIS

Detalhes bibliográficos
Autor(a) principal: STONOGA,Emanuele Therezinha Schueda
Data de Publicação: 2019
Outros Autores: BUENO,Roberta Zawadzki, NAGANO,Thais Ayumi, MARTINS,Vanessa, ROCHA,Sérgio Luiz
Tipo de documento: Artigo
Idioma: eng
Título da fonte: ABCD. Arquivos Brasileiros de Cirurgia Digestiva (São Paulo)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-67202019000200300
Resumo: ABSTRACT Background: Sepsis is an important public health issue and is associated with high treatment costs and high mortality rates. Glutamine supplementation has proven to be beneficial to the functions of the immune system, acting beneficially in the evolution of patients in severe catabolic states. Aim: To evaluate the effect of glutamine supplementation via intraperitoneal in rats, induced sepsis, considering the following organs: intestines, liver, kidneys and lungs. Methods: Male Wistar rats subjected to sepsis by ligature and cecal puncture were divided into two groups: control C (n=6) and glutamine G (n=11), in which were administered dipeptiven 20% at a dose of 2 ml/kg/day (equivalent to 0.4g N(2)-L-alanyl-L-glutamine/kg) intraperitoneally 48 h prior to sepsis induction. After 48 h they were euthanized and intestine, liver, lung and kidney were removed for histological analysis. Results: Intestinal epithelial desquamation of the control group was more intense compared to the glutamine group (p=0.008). In the kidneys, degenerative tubular epithelial changes were less severe in the animals that received glutamine (p=0.029). Regarding to the liver, glutamine group showed lower levels of cell swelling than the control group (p=0.034). In the lung there were no results with statistical significance. Conclusion: Prior intraperitoneal supplementation with glutamine in experimental animals is able to reduce the damage to the intestinal mucosa, to the kidneys and liver’s histoarchitecture.
id CBCD-1_44fa886dbcf2faa7ce1790d86966dc57
oai_identifier_str oai:scielo:S0102-67202019000200300
network_acronym_str CBCD-1
network_name_str ABCD. Arquivos Brasileiros de Cirurgia Digestiva (São Paulo)
repository_id_str
spelling EFFECTS OF INTRAPERITONEAL GLUTAMINE IN THE TREATMENT OF EXPERIMENTAL SEPSISSepsisGlutaminePeritonitisRatsIntestinesABSTRACT Background: Sepsis is an important public health issue and is associated with high treatment costs and high mortality rates. Glutamine supplementation has proven to be beneficial to the functions of the immune system, acting beneficially in the evolution of patients in severe catabolic states. Aim: To evaluate the effect of glutamine supplementation via intraperitoneal in rats, induced sepsis, considering the following organs: intestines, liver, kidneys and lungs. Methods: Male Wistar rats subjected to sepsis by ligature and cecal puncture were divided into two groups: control C (n=6) and glutamine G (n=11), in which were administered dipeptiven 20% at a dose of 2 ml/kg/day (equivalent to 0.4g N(2)-L-alanyl-L-glutamine/kg) intraperitoneally 48 h prior to sepsis induction. After 48 h they were euthanized and intestine, liver, lung and kidney were removed for histological analysis. Results: Intestinal epithelial desquamation of the control group was more intense compared to the glutamine group (p=0.008). In the kidneys, degenerative tubular epithelial changes were less severe in the animals that received glutamine (p=0.029). Regarding to the liver, glutamine group showed lower levels of cell swelling than the control group (p=0.034). In the lung there were no results with statistical significance. Conclusion: Prior intraperitoneal supplementation with glutamine in experimental animals is able to reduce the damage to the intestinal mucosa, to the kidneys and liver’s histoarchitecture.Colégio Brasileiro de Cirurgia Digestiva2019-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-67202019000200300ABCD. Arquivos Brasileiros de Cirurgia Digestiva (São Paulo) v.32 n.2 2019reponame:ABCD. Arquivos Brasileiros de Cirurgia Digestiva (São Paulo)instname:Colégio Brasileiro de Cirurgia Digestiva (CBCD)instacron:CBCD10.1590/0102-672020190001e1431info:eu-repo/semantics/openAccessSTONOGA,Emanuele Therezinha SchuedaBUENO,Roberta ZawadzkiNAGANO,Thais AyumiMARTINS,VanessaROCHA,Sérgio Luizeng2019-04-26T00:00:00Zoai:scielo:S0102-67202019000200300Revistahttp://abarriguda.org.br/revista/index.php/revistaabarrigudaarepb/indexONGhttps://old.scielo.br/oai/scielo-oai.php||revistaabcd@gmail.com2317-63262317-6326opendoar:2019-04-26T00:00ABCD. Arquivos Brasileiros de Cirurgia Digestiva (São Paulo) - Colégio Brasileiro de Cirurgia Digestiva (CBCD)false
dc.title.none.fl_str_mv EFFECTS OF INTRAPERITONEAL GLUTAMINE IN THE TREATMENT OF EXPERIMENTAL SEPSIS
title EFFECTS OF INTRAPERITONEAL GLUTAMINE IN THE TREATMENT OF EXPERIMENTAL SEPSIS
spellingShingle EFFECTS OF INTRAPERITONEAL GLUTAMINE IN THE TREATMENT OF EXPERIMENTAL SEPSIS
STONOGA,Emanuele Therezinha Schueda
Sepsis
Glutamine
Peritonitis
Rats
Intestines
title_short EFFECTS OF INTRAPERITONEAL GLUTAMINE IN THE TREATMENT OF EXPERIMENTAL SEPSIS
title_full EFFECTS OF INTRAPERITONEAL GLUTAMINE IN THE TREATMENT OF EXPERIMENTAL SEPSIS
title_fullStr EFFECTS OF INTRAPERITONEAL GLUTAMINE IN THE TREATMENT OF EXPERIMENTAL SEPSIS
title_full_unstemmed EFFECTS OF INTRAPERITONEAL GLUTAMINE IN THE TREATMENT OF EXPERIMENTAL SEPSIS
title_sort EFFECTS OF INTRAPERITONEAL GLUTAMINE IN THE TREATMENT OF EXPERIMENTAL SEPSIS
author STONOGA,Emanuele Therezinha Schueda
author_facet STONOGA,Emanuele Therezinha Schueda
BUENO,Roberta Zawadzki
NAGANO,Thais Ayumi
MARTINS,Vanessa
ROCHA,Sérgio Luiz
author_role author
author2 BUENO,Roberta Zawadzki
NAGANO,Thais Ayumi
MARTINS,Vanessa
ROCHA,Sérgio Luiz
author2_role author
author
author
author
dc.contributor.author.fl_str_mv STONOGA,Emanuele Therezinha Schueda
BUENO,Roberta Zawadzki
NAGANO,Thais Ayumi
MARTINS,Vanessa
ROCHA,Sérgio Luiz
dc.subject.por.fl_str_mv Sepsis
Glutamine
Peritonitis
Rats
Intestines
topic Sepsis
Glutamine
Peritonitis
Rats
Intestines
description ABSTRACT Background: Sepsis is an important public health issue and is associated with high treatment costs and high mortality rates. Glutamine supplementation has proven to be beneficial to the functions of the immune system, acting beneficially in the evolution of patients in severe catabolic states. Aim: To evaluate the effect of glutamine supplementation via intraperitoneal in rats, induced sepsis, considering the following organs: intestines, liver, kidneys and lungs. Methods: Male Wistar rats subjected to sepsis by ligature and cecal puncture were divided into two groups: control C (n=6) and glutamine G (n=11), in which were administered dipeptiven 20% at a dose of 2 ml/kg/day (equivalent to 0.4g N(2)-L-alanyl-L-glutamine/kg) intraperitoneally 48 h prior to sepsis induction. After 48 h they were euthanized and intestine, liver, lung and kidney were removed for histological analysis. Results: Intestinal epithelial desquamation of the control group was more intense compared to the glutamine group (p=0.008). In the kidneys, degenerative tubular epithelial changes were less severe in the animals that received glutamine (p=0.029). Regarding to the liver, glutamine group showed lower levels of cell swelling than the control group (p=0.034). In the lung there were no results with statistical significance. Conclusion: Prior intraperitoneal supplementation with glutamine in experimental animals is able to reduce the damage to the intestinal mucosa, to the kidneys and liver’s histoarchitecture.
publishDate 2019
dc.date.none.fl_str_mv 2019-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-67202019000200300
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-67202019000200300
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0102-672020190001e1431
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Colégio Brasileiro de Cirurgia Digestiva
publisher.none.fl_str_mv Colégio Brasileiro de Cirurgia Digestiva
dc.source.none.fl_str_mv ABCD. Arquivos Brasileiros de Cirurgia Digestiva (São Paulo) v.32 n.2 2019
reponame:ABCD. Arquivos Brasileiros de Cirurgia Digestiva (São Paulo)
instname:Colégio Brasileiro de Cirurgia Digestiva (CBCD)
instacron:CBCD
instname_str Colégio Brasileiro de Cirurgia Digestiva (CBCD)
instacron_str CBCD
institution CBCD
reponame_str ABCD. Arquivos Brasileiros de Cirurgia Digestiva (São Paulo)
collection ABCD. Arquivos Brasileiros de Cirurgia Digestiva (São Paulo)
repository.name.fl_str_mv ABCD. Arquivos Brasileiros de Cirurgia Digestiva (São Paulo) - Colégio Brasileiro de Cirurgia Digestiva (CBCD)
repository.mail.fl_str_mv ||revistaabcd@gmail.com
_version_ 1754208958660214784