Effects of enteral glutamine treatment on animal model of sepsis

Detalhes bibliográficos
Autor(a) principal: Sulzbacher, Maicon Machado
Data de Publicação: 2018
Outros Autores: Santos, Analú Bender, Basso, Renan Daniel Bueno, Hirsh, Gabriela Elisa, Ludwig, Mirna Stela, Heck, Thiago Gomes
Tipo de documento: Artigo
Idioma: por
Título da fonte: Saúde (Santa Maria)
Texto Completo: https://periodicos.ufsm.br/revistasaude/article/view/31163
Resumo: The aim of study was to evaluate the effects of enteral glutamine treatment on clinical parameters in an animal model of sepsis. Were used 24 mice (B6129SF2/J) in two experiments: First 11 mice were divided into three groups to evaluate the animal model better to simulate the sepsis. Animals received saline (control) or faecal solution 10 or 20% (i.p), and were evaluated glycaemia and rectal temperature for 24h. Second 13 mice were divided into: Control, Sepsis, Glutamine and Sepsis+Glutamine. Glutamine was administered by gavage (1 g/kg) each 4h for 47h. Glycaemia, rectal temperature, leukocyte count and body weight was evaluated. Results: The administration of 20% faecal solution induced hypodynamic phase of sepsis with hypothermia, hypoglycaemia and low leukocyte count. The treatment with glutamine avoids the decrease in leukocyte. Conclusion: Since the immune response is essential in sepsis, the use of glutamine can be useful as adjuvant therapy in sepsis.
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spelling Effects of enteral glutamine treatment on animal model of sepsisEfeitos do tratamento com glutamina via enteral em modelo animal de sepseSepsisAnimal ModelsGlutamineLeukopeniaSepseModelos AnimaisGlutaminaLeucopeniaThe aim of study was to evaluate the effects of enteral glutamine treatment on clinical parameters in an animal model of sepsis. Were used 24 mice (B6129SF2/J) in two experiments: First 11 mice were divided into three groups to evaluate the animal model better to simulate the sepsis. Animals received saline (control) or faecal solution 10 or 20% (i.p), and were evaluated glycaemia and rectal temperature for 24h. Second 13 mice were divided into: Control, Sepsis, Glutamine and Sepsis+Glutamine. Glutamine was administered by gavage (1 g/kg) each 4h for 47h. Glycaemia, rectal temperature, leukocyte count and body weight was evaluated. Results: The administration of 20% faecal solution induced hypodynamic phase of sepsis with hypothermia, hypoglycaemia and low leukocyte count. The treatment with glutamine avoids the decrease in leukocyte. Conclusion: Since the immune response is essential in sepsis, the use of glutamine can be useful as adjuvant therapy in sepsis. Objetivo: Avaliar o tratamento enteral com L-glutamina nos parâmetros clínicos em modelo experimental de sepse. Métodos: Foram utilizados 24 camundongos B6129SF2/J: Primeiramente, 11 camundongos foram divididos em três grupos para avaliar o modelo experimental que mimetize a fase hipodinâmica na sepse. Os animais receberam solução fisiológica ou solução fecal a 10% ou 20% (i.p.), sendo avaliados glicemia e temperatura corporal em 24h. Avaliamos o efeito do tratamento de L-glutamina por 48h na glicemia, temperatura retal, contagem de leucócitos e peso corporal em 13 animais nos grupos: Controle, Sepse, Glutamina e Sepse+Glutamina. Resultados: A solução fecal 20% induziu a sepse, evidenciando hipotermia, hipoglicemia e leucopenia. O tratamento com L-glutamina evitou a leucopenia. Conclusão: O tratamento com L-glutamina previne a leucopenia no modelo experimental de sepse, sem melhorar outros parâmetros clínicos. Sendo a resposta imune essencial na sepse, o uso de glutamina pode ser útil como terapia adjuvante na sepse. Universidade Federal de Santa Maria2018-08-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.ufsm.br/revistasaude/article/view/3116310.5902/2236583431163Saúde (Santa Maria); Revista Saúde (Santa Maria), Vol.44, n.2, maio-ago. 2018Saúde (Santa Maria); Revista Saúde (Santa Maria), Vol.44, n.2, maio-ago. 20182236-58340103-4499reponame:Saúde (Santa Maria)instname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMporhttps://periodicos.ufsm.br/revistasaude/article/view/31163/pdfCopyright (c) 2018 Saúde (Santa Maria)info:eu-repo/semantics/openAccessSulzbacher, Maicon MachadoSantos, Analú BenderBasso, Renan Daniel BuenoHirsh, Gabriela ElisaLudwig, Mirna StelaHeck, Thiago Gomes2020-05-14T20:43:47Zoai:ojs.pkp.sfu.ca:article/31163Revistahttps://periodicos.ufsm.br/revistasaudePUBhttps://periodicos.ufsm.br/revistasaude/oairevistasaude.ufsm@gmail.com || amanda.revsaude@gmail.com || beatriz.revsaude@gmail.com2236-58342236-5834opendoar:2020-05-14T20:43:47Saúde (Santa Maria) - Universidade Federal de Santa Maria (UFSM)false
dc.title.none.fl_str_mv Effects of enteral glutamine treatment on animal model of sepsis
Efeitos do tratamento com glutamina via enteral em modelo animal de sepse
title Effects of enteral glutamine treatment on animal model of sepsis
spellingShingle Effects of enteral glutamine treatment on animal model of sepsis
Sulzbacher, Maicon Machado
Sepsis
Animal Models
Glutamine
Leukopenia
Sepse
Modelos Animais
Glutamina
Leucopenia
title_short Effects of enteral glutamine treatment on animal model of sepsis
title_full Effects of enteral glutamine treatment on animal model of sepsis
title_fullStr Effects of enteral glutamine treatment on animal model of sepsis
title_full_unstemmed Effects of enteral glutamine treatment on animal model of sepsis
title_sort Effects of enteral glutamine treatment on animal model of sepsis
author Sulzbacher, Maicon Machado
author_facet Sulzbacher, Maicon Machado
Santos, Analú Bender
Basso, Renan Daniel Bueno
Hirsh, Gabriela Elisa
Ludwig, Mirna Stela
Heck, Thiago Gomes
author_role author
author2 Santos, Analú Bender
Basso, Renan Daniel Bueno
Hirsh, Gabriela Elisa
Ludwig, Mirna Stela
Heck, Thiago Gomes
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Sulzbacher, Maicon Machado
Santos, Analú Bender
Basso, Renan Daniel Bueno
Hirsh, Gabriela Elisa
Ludwig, Mirna Stela
Heck, Thiago Gomes
dc.subject.por.fl_str_mv Sepsis
Animal Models
Glutamine
Leukopenia
Sepse
Modelos Animais
Glutamina
Leucopenia
topic Sepsis
Animal Models
Glutamine
Leukopenia
Sepse
Modelos Animais
Glutamina
Leucopenia
description The aim of study was to evaluate the effects of enteral glutamine treatment on clinical parameters in an animal model of sepsis. Were used 24 mice (B6129SF2/J) in two experiments: First 11 mice were divided into three groups to evaluate the animal model better to simulate the sepsis. Animals received saline (control) or faecal solution 10 or 20% (i.p), and were evaluated glycaemia and rectal temperature for 24h. Second 13 mice were divided into: Control, Sepsis, Glutamine and Sepsis+Glutamine. Glutamine was administered by gavage (1 g/kg) each 4h for 47h. Glycaemia, rectal temperature, leukocyte count and body weight was evaluated. Results: The administration of 20% faecal solution induced hypodynamic phase of sepsis with hypothermia, hypoglycaemia and low leukocyte count. The treatment with glutamine avoids the decrease in leukocyte. Conclusion: Since the immune response is essential in sepsis, the use of glutamine can be useful as adjuvant therapy in sepsis.
publishDate 2018
dc.date.none.fl_str_mv 2018-08-15
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://periodicos.ufsm.br/revistasaude/article/view/31163
10.5902/2236583431163
url https://periodicos.ufsm.br/revistasaude/article/view/31163
identifier_str_mv 10.5902/2236583431163
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://periodicos.ufsm.br/revistasaude/article/view/31163/pdf
dc.rights.driver.fl_str_mv Copyright (c) 2018 Saúde (Santa Maria)
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2018 Saúde (Santa Maria)
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
publisher.none.fl_str_mv Universidade Federal de Santa Maria
dc.source.none.fl_str_mv Saúde (Santa Maria); Revista Saúde (Santa Maria), Vol.44, n.2, maio-ago. 2018
Saúde (Santa Maria); Revista Saúde (Santa Maria), Vol.44, n.2, maio-ago. 2018
2236-5834
0103-4499
reponame:Saúde (Santa Maria)
instname:Universidade Federal de Santa Maria (UFSM)
instacron:UFSM
instname_str Universidade Federal de Santa Maria (UFSM)
instacron_str UFSM
institution UFSM
reponame_str Saúde (Santa Maria)
collection Saúde (Santa Maria)
repository.name.fl_str_mv Saúde (Santa Maria) - Universidade Federal de Santa Maria (UFSM)
repository.mail.fl_str_mv revistasaude.ufsm@gmail.com || amanda.revsaude@gmail.com || beatriz.revsaude@gmail.com
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