Real-time PCR in infectious uveitis as an alternative diagnosis
Autor(a) principal: | |
---|---|
Data de Publicação: | 2011 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Arquivos brasileiros de oftalmologia (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-27492011000400006 |
Resumo: | PURPOSE: Uveitis is a major visual impairment disease affecting parts or the entire uveal tract and occasionally the sclera, the cornea or the optic nerve. The disease is a major cause of ocular morbidity and blindness in immunocompetent and immunocompromised patients. In this work we analyzed the sensitivity and specificity of real-time PCR to detect the etiological agent from blood, plasma, vitreous and aqueous humor and compared with the diagnostic hypothesis. METHODS: Twenty-seven patients (13 male) were studied and Real-time PCR method was used for the detection of herpes simplex virus 1 (HSV-1), herpes simplex virus 2 (HSV-2), varicella zoster virus (VZV), cytomegalovirus (CMV), Mycobacterium tuberculosis (TB) and Toxoplasma gondii (Toxo) in the aqueous humor as well as in the vitreous, blood and plasma. RESULTS: Our results showed the presence of Toxo, CMV, VZV or HSV-2 in 19.2% of aqueous humor samples, and in 30% of vitreous humor samples. In plasma and blood samples, only CMV was detected (11.1% and 3.7%, respectively). CONCLUSION: Real-time PCR was able to detect and to confirm diagnostic hypothesis in uveitis. Our data also confirms that vitreous humor is the best source for molecular diagnosis of infectious uveitis but indicates aqueous humor samples that are easier to obtain may also be appropriate to be tested by Real-time PCR. |
id |
CBO-2_8798eea5f215b94da4c28028cea184d4 |
---|---|
oai_identifier_str |
oai:scielo:S0004-27492011000400006 |
network_acronym_str |
CBO-2 |
network_name_str |
Arquivos brasileiros de oftalmologia (Online) |
repository_id_str |
|
spelling |
Real-time PCR in infectious uveitis as an alternative diagnosisPolymerase chain reaction/methodsUveitis/diagnosisUveitis/microbiologyUveitis/virologyEye infections, viral/microbiologyAqueous humorVitreous bodyPURPOSE: Uveitis is a major visual impairment disease affecting parts or the entire uveal tract and occasionally the sclera, the cornea or the optic nerve. The disease is a major cause of ocular morbidity and blindness in immunocompetent and immunocompromised patients. In this work we analyzed the sensitivity and specificity of real-time PCR to detect the etiological agent from blood, plasma, vitreous and aqueous humor and compared with the diagnostic hypothesis. METHODS: Twenty-seven patients (13 male) were studied and Real-time PCR method was used for the detection of herpes simplex virus 1 (HSV-1), herpes simplex virus 2 (HSV-2), varicella zoster virus (VZV), cytomegalovirus (CMV), Mycobacterium tuberculosis (TB) and Toxoplasma gondii (Toxo) in the aqueous humor as well as in the vitreous, blood and plasma. RESULTS: Our results showed the presence of Toxo, CMV, VZV or HSV-2 in 19.2% of aqueous humor samples, and in 30% of vitreous humor samples. In plasma and blood samples, only CMV was detected (11.1% and 3.7%, respectively). CONCLUSION: Real-time PCR was able to detect and to confirm diagnostic hypothesis in uveitis. Our data also confirms that vitreous humor is the best source for molecular diagnosis of infectious uveitis but indicates aqueous humor samples that are easier to obtain may also be appropriate to be tested by Real-time PCR.Conselho Brasileiro de Oftalmologia2011-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-27492011000400006Arquivos Brasileiros de Oftalmologia v.74 n.4 2011reponame:Arquivos brasileiros de oftalmologia (Online)instname:Conselho Brasileiro de Oftalmologia (CBO)instacron:CBO10.1590/S0004-27492011000400006info:eu-repo/semantics/openAccessSantos,Fabio Felipe dosCommodaro,Alessandra GonçalvesSouza,Andrea Vieira dePinho,João Renato RebelloSitnik,RobertaGarcia,ClaudioRibeiro,Ana Lúcia PereiraMuccioli,CristinaLottenberg,Cláudio LuizRizzo,Luiz VicenteBelfort Junior,Rubenseng2011-11-04T00:00:00Zoai:scielo:S0004-27492011000400006Revistahttp://aboonline.org.br/https://old.scielo.br/oai/scielo-oai.phpaboonline@cbo.com.br||abo@cbo.com.br1678-29250004-2749opendoar:2011-11-04T00:00Arquivos brasileiros de oftalmologia (Online) - Conselho Brasileiro de Oftalmologia (CBO)false |
dc.title.none.fl_str_mv |
Real-time PCR in infectious uveitis as an alternative diagnosis |
title |
Real-time PCR in infectious uveitis as an alternative diagnosis |
spellingShingle |
Real-time PCR in infectious uveitis as an alternative diagnosis Santos,Fabio Felipe dos Polymerase chain reaction/methods Uveitis/diagnosis Uveitis/microbiology Uveitis/virology Eye infections, viral/microbiology Aqueous humor Vitreous body |
title_short |
Real-time PCR in infectious uveitis as an alternative diagnosis |
title_full |
Real-time PCR in infectious uveitis as an alternative diagnosis |
title_fullStr |
Real-time PCR in infectious uveitis as an alternative diagnosis |
title_full_unstemmed |
Real-time PCR in infectious uveitis as an alternative diagnosis |
title_sort |
Real-time PCR in infectious uveitis as an alternative diagnosis |
author |
Santos,Fabio Felipe dos |
author_facet |
Santos,Fabio Felipe dos Commodaro,Alessandra Gonçalves Souza,Andrea Vieira de Pinho,João Renato Rebello Sitnik,Roberta Garcia,Claudio Ribeiro,Ana Lúcia Pereira Muccioli,Cristina Lottenberg,Cláudio Luiz Rizzo,Luiz Vicente Belfort Junior,Rubens |
author_role |
author |
author2 |
Commodaro,Alessandra Gonçalves Souza,Andrea Vieira de Pinho,João Renato Rebello Sitnik,Roberta Garcia,Claudio Ribeiro,Ana Lúcia Pereira Muccioli,Cristina Lottenberg,Cláudio Luiz Rizzo,Luiz Vicente Belfort Junior,Rubens |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Santos,Fabio Felipe dos Commodaro,Alessandra Gonçalves Souza,Andrea Vieira de Pinho,João Renato Rebello Sitnik,Roberta Garcia,Claudio Ribeiro,Ana Lúcia Pereira Muccioli,Cristina Lottenberg,Cláudio Luiz Rizzo,Luiz Vicente Belfort Junior,Rubens |
dc.subject.por.fl_str_mv |
Polymerase chain reaction/methods Uveitis/diagnosis Uveitis/microbiology Uveitis/virology Eye infections, viral/microbiology Aqueous humor Vitreous body |
topic |
Polymerase chain reaction/methods Uveitis/diagnosis Uveitis/microbiology Uveitis/virology Eye infections, viral/microbiology Aqueous humor Vitreous body |
description |
PURPOSE: Uveitis is a major visual impairment disease affecting parts or the entire uveal tract and occasionally the sclera, the cornea or the optic nerve. The disease is a major cause of ocular morbidity and blindness in immunocompetent and immunocompromised patients. In this work we analyzed the sensitivity and specificity of real-time PCR to detect the etiological agent from blood, plasma, vitreous and aqueous humor and compared with the diagnostic hypothesis. METHODS: Twenty-seven patients (13 male) were studied and Real-time PCR method was used for the detection of herpes simplex virus 1 (HSV-1), herpes simplex virus 2 (HSV-2), varicella zoster virus (VZV), cytomegalovirus (CMV), Mycobacterium tuberculosis (TB) and Toxoplasma gondii (Toxo) in the aqueous humor as well as in the vitreous, blood and plasma. RESULTS: Our results showed the presence of Toxo, CMV, VZV or HSV-2 in 19.2% of aqueous humor samples, and in 30% of vitreous humor samples. In plasma and blood samples, only CMV was detected (11.1% and 3.7%, respectively). CONCLUSION: Real-time PCR was able to detect and to confirm diagnostic hypothesis in uveitis. Our data also confirms that vitreous humor is the best source for molecular diagnosis of infectious uveitis but indicates aqueous humor samples that are easier to obtain may also be appropriate to be tested by Real-time PCR. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-08-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-27492011000400006 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-27492011000400006 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0004-27492011000400006 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Conselho Brasileiro de Oftalmologia |
publisher.none.fl_str_mv |
Conselho Brasileiro de Oftalmologia |
dc.source.none.fl_str_mv |
Arquivos Brasileiros de Oftalmologia v.74 n.4 2011 reponame:Arquivos brasileiros de oftalmologia (Online) instname:Conselho Brasileiro de Oftalmologia (CBO) instacron:CBO |
instname_str |
Conselho Brasileiro de Oftalmologia (CBO) |
instacron_str |
CBO |
institution |
CBO |
reponame_str |
Arquivos brasileiros de oftalmologia (Online) |
collection |
Arquivos brasileiros de oftalmologia (Online) |
repository.name.fl_str_mv |
Arquivos brasileiros de oftalmologia (Online) - Conselho Brasileiro de Oftalmologia (CBO) |
repository.mail.fl_str_mv |
aboonline@cbo.com.br||abo@cbo.com.br |
_version_ |
1754209026867986432 |