Evaluation of microbial contamination in multi-dose fluorescein eyedrops in a reference eye center
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Arquivos brasileiros de oftalmologia (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-27492021005007201 |
Resumo: | ABSTRACT Purpose: To analyze the presence of microorganisms in fluorescein eyedrops used in a reference eye center in Recife-PE. Methods: This real-life and masked study evaluated fluorescein eyedrops used at the Altino Ventura Foundation in May 2019. Cultures were performed according to exposure times; I) three eyedrop bottles were analyzed after one day of use, II) three eyedrop bottles after 4 d of use, III) three eyedrop bottles after 8 d of use, and IV) three unopened bottles used as control. Samples were collected from the bottle's tip, instilled drop, and residual fluid. After incubation, all colonies were analyzed and identified through biochemical tests. Results: The contamination rate of the fluorescein eyedrop bottles in this study was 55.5% (5/9 vials). There was no contamination in the control group. The highest contamination was seen in one day exposed eyedrops, in 100% of the bottles. The bottle's tip had a higher rate of contamination compared to the drop and residual fluid. Gram-positive bacteria were isolated in 7/27 (25.9%) samples. Growth of fungi or gram-negative bacteria was not observed. Conclusion: The identification of gram-positive bacteria predominantly on the tip of the fluorescein eyedrop bottles suggests inadequate handling as the main cause of contamination. |
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Arquivos brasileiros de oftalmologia (Online) |
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Evaluation of microbial contamination in multi-dose fluorescein eyedrops in a reference eye centerFluoresceinOphthalmic solutionDrug contaminationEye infection, bacterial/microbiologyBacteria/isolation & purificationABSTRACT Purpose: To analyze the presence of microorganisms in fluorescein eyedrops used in a reference eye center in Recife-PE. Methods: This real-life and masked study evaluated fluorescein eyedrops used at the Altino Ventura Foundation in May 2019. Cultures were performed according to exposure times; I) three eyedrop bottles were analyzed after one day of use, II) three eyedrop bottles after 4 d of use, III) three eyedrop bottles after 8 d of use, and IV) three unopened bottles used as control. Samples were collected from the bottle's tip, instilled drop, and residual fluid. After incubation, all colonies were analyzed and identified through biochemical tests. Results: The contamination rate of the fluorescein eyedrop bottles in this study was 55.5% (5/9 vials). There was no contamination in the control group. The highest contamination was seen in one day exposed eyedrops, in 100% of the bottles. The bottle's tip had a higher rate of contamination compared to the drop and residual fluid. Gram-positive bacteria were isolated in 7/27 (25.9%) samples. Growth of fungi or gram-negative bacteria was not observed. Conclusion: The identification of gram-positive bacteria predominantly on the tip of the fluorescein eyedrop bottles suggests inadequate handling as the main cause of contamination.Conselho Brasileiro de Oftalmologia2021-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-27492021005007201Arquivos Brasileiros de Oftalmologia n.ahead 2021reponame:Arquivos brasileiros de oftalmologia (Online)instname:Conselho Brasileiro de Oftalmologia (CBO)instacron:CBO10.5935/0004-2749.20210070info:eu-repo/semantics/openAccessCosta,Camila de MoraesEspósito,Kayo PinheiroFlorêncio,Victor Medeiros BrandãoAquino,Paulo Romero LeiteFirmo,Elza FerreiraCosta,Iggor Macêdo do AmaralRocha,CamillaAndrade,Camila Galvão deOliveira-Júnior,Jorge BelémVentura,Camila V.eng2021-09-23T00:00:00Zoai:scielo:S0004-27492021005007201Revistahttp://aboonline.org.br/https://old.scielo.br/oai/scielo-oai.phpaboonline@cbo.com.br||abo@cbo.com.br1678-29250004-2749opendoar:2021-09-23T00:00Arquivos brasileiros de oftalmologia (Online) - Conselho Brasileiro de Oftalmologia (CBO)false |
dc.title.none.fl_str_mv |
Evaluation of microbial contamination in multi-dose fluorescein eyedrops in a reference eye center |
title |
Evaluation of microbial contamination in multi-dose fluorescein eyedrops in a reference eye center |
spellingShingle |
Evaluation of microbial contamination in multi-dose fluorescein eyedrops in a reference eye center Costa,Camila de Moraes Fluorescein Ophthalmic solution Drug contamination Eye infection, bacterial/microbiology Bacteria/isolation & purification |
title_short |
Evaluation of microbial contamination in multi-dose fluorescein eyedrops in a reference eye center |
title_full |
Evaluation of microbial contamination in multi-dose fluorescein eyedrops in a reference eye center |
title_fullStr |
Evaluation of microbial contamination in multi-dose fluorescein eyedrops in a reference eye center |
title_full_unstemmed |
Evaluation of microbial contamination in multi-dose fluorescein eyedrops in a reference eye center |
title_sort |
Evaluation of microbial contamination in multi-dose fluorescein eyedrops in a reference eye center |
author |
Costa,Camila de Moraes |
author_facet |
Costa,Camila de Moraes Espósito,Kayo Pinheiro Florêncio,Victor Medeiros Brandão Aquino,Paulo Romero Leite Firmo,Elza Ferreira Costa,Iggor Macêdo do Amaral Rocha,Camilla Andrade,Camila Galvão de Oliveira-Júnior,Jorge Belém Ventura,Camila V. |
author_role |
author |
author2 |
Espósito,Kayo Pinheiro Florêncio,Victor Medeiros Brandão Aquino,Paulo Romero Leite Firmo,Elza Ferreira Costa,Iggor Macêdo do Amaral Rocha,Camilla Andrade,Camila Galvão de Oliveira-Júnior,Jorge Belém Ventura,Camila V. |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Costa,Camila de Moraes Espósito,Kayo Pinheiro Florêncio,Victor Medeiros Brandão Aquino,Paulo Romero Leite Firmo,Elza Ferreira Costa,Iggor Macêdo do Amaral Rocha,Camilla Andrade,Camila Galvão de Oliveira-Júnior,Jorge Belém Ventura,Camila V. |
dc.subject.por.fl_str_mv |
Fluorescein Ophthalmic solution Drug contamination Eye infection, bacterial/microbiology Bacteria/isolation & purification |
topic |
Fluorescein Ophthalmic solution Drug contamination Eye infection, bacterial/microbiology Bacteria/isolation & purification |
description |
ABSTRACT Purpose: To analyze the presence of microorganisms in fluorescein eyedrops used in a reference eye center in Recife-PE. Methods: This real-life and masked study evaluated fluorescein eyedrops used at the Altino Ventura Foundation in May 2019. Cultures were performed according to exposure times; I) three eyedrop bottles were analyzed after one day of use, II) three eyedrop bottles after 4 d of use, III) three eyedrop bottles after 8 d of use, and IV) three unopened bottles used as control. Samples were collected from the bottle's tip, instilled drop, and residual fluid. After incubation, all colonies were analyzed and identified through biochemical tests. Results: The contamination rate of the fluorescein eyedrop bottles in this study was 55.5% (5/9 vials). There was no contamination in the control group. The highest contamination was seen in one day exposed eyedrops, in 100% of the bottles. The bottle's tip had a higher rate of contamination compared to the drop and residual fluid. Gram-positive bacteria were isolated in 7/27 (25.9%) samples. Growth of fungi or gram-negative bacteria was not observed. Conclusion: The identification of gram-positive bacteria predominantly on the tip of the fluorescein eyedrop bottles suggests inadequate handling as the main cause of contamination. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-27492021005007201 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-27492021005007201 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.5935/0004-2749.20210070 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Conselho Brasileiro de Oftalmologia |
publisher.none.fl_str_mv |
Conselho Brasileiro de Oftalmologia |
dc.source.none.fl_str_mv |
Arquivos Brasileiros de Oftalmologia n.ahead 2021 reponame:Arquivos brasileiros de oftalmologia (Online) instname:Conselho Brasileiro de Oftalmologia (CBO) instacron:CBO |
instname_str |
Conselho Brasileiro de Oftalmologia (CBO) |
instacron_str |
CBO |
institution |
CBO |
reponame_str |
Arquivos brasileiros de oftalmologia (Online) |
collection |
Arquivos brasileiros de oftalmologia (Online) |
repository.name.fl_str_mv |
Arquivos brasileiros de oftalmologia (Online) - Conselho Brasileiro de Oftalmologia (CBO) |
repository.mail.fl_str_mv |
aboonline@cbo.com.br||abo@cbo.com.br |
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1754209031706116096 |