Design of primer pairs for species-specific diagnosis of Leishmania (Leishmania) infantum chagasi using PCR

Detalhes bibliográficos
Autor(a) principal: Silveira Neto,Osvaldo José da
Data de Publicação: 2012
Outros Autores: Duarte,Sabrina Castilho, Costa,Hérika Xavier da, Linhares,Guido Fontgalland Coelho
Tipo de documento: Relatório
Idioma: eng
Título da fonte: Revista Brasileira de Parasitologia Veterinária (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612012000300024
Resumo: The objective of this study was to design and evaluate new primers for species-specific detection of L. infantum chagasi using PCR. Two combinations of primer pairs were established with the aim of obtaining specific amplification products from the L. infantum chagasi 18S rRNA gene. The combinations of the primer pairs and the respective sizes of the PCR products, based on the U422465 GenBank reference sequence of L. infantum chagasi, were: LCS1/LCS3 (259 bp) and LCS2/LCS3 (820 bp). It was concluded that the new PCR assays optimized using the primer pairs LCS1/LCS3 and LCS2/LCS3 were effective for specific detection of L. infantum chagasi, with analytical sensitivity to detect 1 pg/µL of DNA.
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spelling Design of primer pairs for species-specific diagnosis of Leishmania (Leishmania) infantum chagasi using PCRKala-azarvisceral leishmaniasismolecular diagnosis18S rRNA geneL. infantum chagasiThe objective of this study was to design and evaluate new primers for species-specific detection of L. infantum chagasi using PCR. Two combinations of primer pairs were established with the aim of obtaining specific amplification products from the L. infantum chagasi 18S rRNA gene. The combinations of the primer pairs and the respective sizes of the PCR products, based on the U422465 GenBank reference sequence of L. infantum chagasi, were: LCS1/LCS3 (259 bp) and LCS2/LCS3 (820 bp). It was concluded that the new PCR assays optimized using the primer pairs LCS1/LCS3 and LCS2/LCS3 were effective for specific detection of L. infantum chagasi, with analytical sensitivity to detect 1 pg/µL of DNA.Colégio Brasileiro de Parasitologia Veterinária2012-09-01info:eu-repo/semantics/reportinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612012000300024Revista Brasileira de Parasitologia Veterinária v.21 n.3 2012reponame:Revista Brasileira de Parasitologia Veterinária (Online)instname:Colégio Brasileiro de Parasitologia Veterinária (CBPV)instacron:CBPV10.1590/S1984-29612012000300024info:eu-repo/semantics/openAccessSilveira Neto,Osvaldo José daDuarte,Sabrina CastilhoCosta,Hérika Xavier daLinhares,Guido Fontgalland Coelhoeng2012-10-11T00:00:00Zoai:scielo:S1984-29612012000300024Revistahttp://www.scielo.br/scielo.php?script=sci_serial&lng=pt&pid=1984-2961https://old.scielo.br/oai/scielo-oai.php||zacariascbpv@fcav.unesp.br1984-29610103-846Xopendoar:2012-10-11T00:00Revista Brasileira de Parasitologia Veterinária (Online) - Colégio Brasileiro de Parasitologia Veterinária (CBPV)false
dc.title.none.fl_str_mv Design of primer pairs for species-specific diagnosis of Leishmania (Leishmania) infantum chagasi using PCR
title Design of primer pairs for species-specific diagnosis of Leishmania (Leishmania) infantum chagasi using PCR
spellingShingle Design of primer pairs for species-specific diagnosis of Leishmania (Leishmania) infantum chagasi using PCR
Silveira Neto,Osvaldo José da
Kala-azar
visceral leishmaniasis
molecular diagnosis
18S rRNA gene
L. infantum chagasi
title_short Design of primer pairs for species-specific diagnosis of Leishmania (Leishmania) infantum chagasi using PCR
title_full Design of primer pairs for species-specific diagnosis of Leishmania (Leishmania) infantum chagasi using PCR
title_fullStr Design of primer pairs for species-specific diagnosis of Leishmania (Leishmania) infantum chagasi using PCR
title_full_unstemmed Design of primer pairs for species-specific diagnosis of Leishmania (Leishmania) infantum chagasi using PCR
title_sort Design of primer pairs for species-specific diagnosis of Leishmania (Leishmania) infantum chagasi using PCR
author Silveira Neto,Osvaldo José da
author_facet Silveira Neto,Osvaldo José da
Duarte,Sabrina Castilho
Costa,Hérika Xavier da
Linhares,Guido Fontgalland Coelho
author_role author
author2 Duarte,Sabrina Castilho
Costa,Hérika Xavier da
Linhares,Guido Fontgalland Coelho
author2_role author
author
author
dc.contributor.author.fl_str_mv Silveira Neto,Osvaldo José da
Duarte,Sabrina Castilho
Costa,Hérika Xavier da
Linhares,Guido Fontgalland Coelho
dc.subject.por.fl_str_mv Kala-azar
visceral leishmaniasis
molecular diagnosis
18S rRNA gene
L. infantum chagasi
topic Kala-azar
visceral leishmaniasis
molecular diagnosis
18S rRNA gene
L. infantum chagasi
description The objective of this study was to design and evaluate new primers for species-specific detection of L. infantum chagasi using PCR. Two combinations of primer pairs were established with the aim of obtaining specific amplification products from the L. infantum chagasi 18S rRNA gene. The combinations of the primer pairs and the respective sizes of the PCR products, based on the U422465 GenBank reference sequence of L. infantum chagasi, were: LCS1/LCS3 (259 bp) and LCS2/LCS3 (820 bp). It was concluded that the new PCR assays optimized using the primer pairs LCS1/LCS3 and LCS2/LCS3 were effective for specific detection of L. infantum chagasi, with analytical sensitivity to detect 1 pg/µL of DNA.
publishDate 2012
dc.date.none.fl_str_mv 2012-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/report
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format report
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612012000300024
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612012000300024
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1984-29612012000300024
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Colégio Brasileiro de Parasitologia Veterinária
publisher.none.fl_str_mv Colégio Brasileiro de Parasitologia Veterinária
dc.source.none.fl_str_mv Revista Brasileira de Parasitologia Veterinária v.21 n.3 2012
reponame:Revista Brasileira de Parasitologia Veterinária (Online)
instname:Colégio Brasileiro de Parasitologia Veterinária (CBPV)
instacron:CBPV
instname_str Colégio Brasileiro de Parasitologia Veterinária (CBPV)
instacron_str CBPV
institution CBPV
reponame_str Revista Brasileira de Parasitologia Veterinária (Online)
collection Revista Brasileira de Parasitologia Veterinária (Online)
repository.name.fl_str_mv Revista Brasileira de Parasitologia Veterinária (Online) - Colégio Brasileiro de Parasitologia Veterinária (CBPV)
repository.mail.fl_str_mv ||zacariascbpv@fcav.unesp.br
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