The effect of water source and soil supplementation on parasite contamination in organic vegetable gardens
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista Brasileira de Parasitologia Veterinária (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612018000300327 |
Resumo: | Abstract The objective of this study was to determine factors associated with vegetable contamination with zoonotic protozoan. Samples of water, soil and vegetables were collected from July/2014 to May/2016, totaling 83 samples, 21 properties of Londrina region, Paraná, Brazil. DNA amplification of Toxoplasma gondii, Cryptosporidium spp. and Giardia intestinalis in the samples was conducted using polymerase chain reaction (PCR). The PCR results were positive for T. gondii in 12.9% (8/62), Cryptosporidium spp. in 11.3% (7/62) and G. intestinalis in 25.8% (16/62) of the samples. DNA sequencing identified C. parvum in five samples and G. intestinalis Assemblage E in three. The statistical associations demonstrated greater probability of positive samples for T. gondii and for at least one of the three protozoa when the source of irrigation water was the river; a greater chance of positive samples for Cryptosporidium spp. when deer were present on the property; and a smaller chance of positive samples for at least one of the three etiologic agents when soil was supplemented with limestone. The results expose some critical contamination points, providing support for training farmers on good management practices during the production process. |
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The effect of water source and soil supplementation on parasite contamination in organic vegetable gardensVegetableswaterenvironmental contaminationCryptosporidium spp.Toxoplasma gondiiGiardia intestinalisAbstract The objective of this study was to determine factors associated with vegetable contamination with zoonotic protozoan. Samples of water, soil and vegetables were collected from July/2014 to May/2016, totaling 83 samples, 21 properties of Londrina region, Paraná, Brazil. DNA amplification of Toxoplasma gondii, Cryptosporidium spp. and Giardia intestinalis in the samples was conducted using polymerase chain reaction (PCR). The PCR results were positive for T. gondii in 12.9% (8/62), Cryptosporidium spp. in 11.3% (7/62) and G. intestinalis in 25.8% (16/62) of the samples. DNA sequencing identified C. parvum in five samples and G. intestinalis Assemblage E in three. The statistical associations demonstrated greater probability of positive samples for T. gondii and for at least one of the three protozoa when the source of irrigation water was the river; a greater chance of positive samples for Cryptosporidium spp. when deer were present on the property; and a smaller chance of positive samples for at least one of the three etiologic agents when soil was supplemented with limestone. The results expose some critical contamination points, providing support for training farmers on good management practices during the production process.Colégio Brasileiro de Parasitologia Veterinária2018-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612018000300327Revista Brasileira de Parasitologia Veterinária v.27 n.3 2018reponame:Revista Brasileira de Parasitologia Veterinária (Online)instname:Colégio Brasileiro de Parasitologia Veterinária (CBPV)instacron:CBPV10.1590/s1984-296120180050info:eu-repo/semantics/openAccessFerreira,Fernanda PintoCaldart,Eloiza TelesFreire,Roberta LemosMitsuka-Breganó,ReginaFreitas,Felipe Machado deMiura,Ana CarolinaMareze,MarcelleMartins,Felippe Danyel CardosoUrbano,Mariana RagassiSeifert,Adilson LuizNavarro,Italmar Teodoricoeng2018-09-17T00:00:00Zoai:scielo:S1984-29612018000300327Revistahttp://www.scielo.br/scielo.php?script=sci_serial&lng=pt&pid=1984-2961https://old.scielo.br/oai/scielo-oai.php||zacariascbpv@fcav.unesp.br1984-29610103-846Xopendoar:2018-09-17T00:00Revista Brasileira de Parasitologia Veterinária (Online) - Colégio Brasileiro de Parasitologia Veterinária (CBPV)false |
dc.title.none.fl_str_mv |
The effect of water source and soil supplementation on parasite contamination in organic vegetable gardens |
title |
The effect of water source and soil supplementation on parasite contamination in organic vegetable gardens |
spellingShingle |
The effect of water source and soil supplementation on parasite contamination in organic vegetable gardens Ferreira,Fernanda Pinto Vegetables water environmental contamination Cryptosporidium spp. Toxoplasma gondii Giardia intestinalis |
title_short |
The effect of water source and soil supplementation on parasite contamination in organic vegetable gardens |
title_full |
The effect of water source and soil supplementation on parasite contamination in organic vegetable gardens |
title_fullStr |
The effect of water source and soil supplementation on parasite contamination in organic vegetable gardens |
title_full_unstemmed |
The effect of water source and soil supplementation on parasite contamination in organic vegetable gardens |
title_sort |
The effect of water source and soil supplementation on parasite contamination in organic vegetable gardens |
author |
Ferreira,Fernanda Pinto |
author_facet |
Ferreira,Fernanda Pinto Caldart,Eloiza Teles Freire,Roberta Lemos Mitsuka-Breganó,Regina Freitas,Felipe Machado de Miura,Ana Carolina Mareze,Marcelle Martins,Felippe Danyel Cardoso Urbano,Mariana Ragassi Seifert,Adilson Luiz Navarro,Italmar Teodorico |
author_role |
author |
author2 |
Caldart,Eloiza Teles Freire,Roberta Lemos Mitsuka-Breganó,Regina Freitas,Felipe Machado de Miura,Ana Carolina Mareze,Marcelle Martins,Felippe Danyel Cardoso Urbano,Mariana Ragassi Seifert,Adilson Luiz Navarro,Italmar Teodorico |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Ferreira,Fernanda Pinto Caldart,Eloiza Teles Freire,Roberta Lemos Mitsuka-Breganó,Regina Freitas,Felipe Machado de Miura,Ana Carolina Mareze,Marcelle Martins,Felippe Danyel Cardoso Urbano,Mariana Ragassi Seifert,Adilson Luiz Navarro,Italmar Teodorico |
dc.subject.por.fl_str_mv |
Vegetables water environmental contamination Cryptosporidium spp. Toxoplasma gondii Giardia intestinalis |
topic |
Vegetables water environmental contamination Cryptosporidium spp. Toxoplasma gondii Giardia intestinalis |
description |
Abstract The objective of this study was to determine factors associated with vegetable contamination with zoonotic protozoan. Samples of water, soil and vegetables were collected from July/2014 to May/2016, totaling 83 samples, 21 properties of Londrina region, Paraná, Brazil. DNA amplification of Toxoplasma gondii, Cryptosporidium spp. and Giardia intestinalis in the samples was conducted using polymerase chain reaction (PCR). The PCR results were positive for T. gondii in 12.9% (8/62), Cryptosporidium spp. in 11.3% (7/62) and G. intestinalis in 25.8% (16/62) of the samples. DNA sequencing identified C. parvum in five samples and G. intestinalis Assemblage E in three. The statistical associations demonstrated greater probability of positive samples for T. gondii and for at least one of the three protozoa when the source of irrigation water was the river; a greater chance of positive samples for Cryptosporidium spp. when deer were present on the property; and a smaller chance of positive samples for at least one of the three etiologic agents when soil was supplemented with limestone. The results expose some critical contamination points, providing support for training farmers on good management practices during the production process. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612018000300327 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612018000300327 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/s1984-296120180050 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Colégio Brasileiro de Parasitologia Veterinária |
publisher.none.fl_str_mv |
Colégio Brasileiro de Parasitologia Veterinária |
dc.source.none.fl_str_mv |
Revista Brasileira de Parasitologia Veterinária v.27 n.3 2018 reponame:Revista Brasileira de Parasitologia Veterinária (Online) instname:Colégio Brasileiro de Parasitologia Veterinária (CBPV) instacron:CBPV |
instname_str |
Colégio Brasileiro de Parasitologia Veterinária (CBPV) |
instacron_str |
CBPV |
institution |
CBPV |
reponame_str |
Revista Brasileira de Parasitologia Veterinária (Online) |
collection |
Revista Brasileira de Parasitologia Veterinária (Online) |
repository.name.fl_str_mv |
Revista Brasileira de Parasitologia Veterinária (Online) - Colégio Brasileiro de Parasitologia Veterinária (CBPV) |
repository.mail.fl_str_mv |
||zacariascbpv@fcav.unesp.br |
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1754208917481586688 |