rROP2 from Toxoplasma gondii as a potential vaccine against oocyst shedding in domestic cats
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista Brasileira de Parasitologia Veterinária (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612017000100067 |
Resumo: | Abstract The aim of the present study was to evaluate oocyst shedding in cats immunized by nasal route with T. gondii proteins ROP2. Twelve short hair cats (Felis catus) were divided in three groups G1, G2 and G3 (n=4). Animals from G1 received 100 μg of rROP2 proteins plus 20 μg of Quil-A, G2 received 100 μg of BSA plus 20 μg of Quil-A, and the G3 only saline solution (control group). All treatments were done by intranasal route at days 0, 21, 42, and 63. The challenge was performed in all groups on day 70 with ≅ 800 tissue cysts of ME-49 strain by oral route. Animals from G1 shed less oocysts (86.7%) than control groups. ELISA was used to detect anti-rROP2 IgG and IgA, however, there were no correlation between number of oocyst shedding by either IgG or IgA antibody levels. In the present work, in spite of lesser oocysts production in immunized group than control groups, it was not possible to associate the use of rROP2 via nostrils with protection against oocyst shedding. For the future, the use of either other recombinant proteins or DNA vaccine, in combination with rROP2 could be tested to try improving the efficacy of this kind of vaccine. |
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rROP2 from Toxoplasma gondii as a potential vaccine against oocyst shedding in domestic catsDefinitive hostimmunizationrecombinant proteintoxoplasmosisAbstract The aim of the present study was to evaluate oocyst shedding in cats immunized by nasal route with T. gondii proteins ROP2. Twelve short hair cats (Felis catus) were divided in three groups G1, G2 and G3 (n=4). Animals from G1 received 100 μg of rROP2 proteins plus 20 μg of Quil-A, G2 received 100 μg of BSA plus 20 μg of Quil-A, and the G3 only saline solution (control group). All treatments were done by intranasal route at days 0, 21, 42, and 63. The challenge was performed in all groups on day 70 with ≅ 800 tissue cysts of ME-49 strain by oral route. Animals from G1 shed less oocysts (86.7%) than control groups. ELISA was used to detect anti-rROP2 IgG and IgA, however, there were no correlation between number of oocyst shedding by either IgG or IgA antibody levels. In the present work, in spite of lesser oocysts production in immunized group than control groups, it was not possible to associate the use of rROP2 via nostrils with protection against oocyst shedding. For the future, the use of either other recombinant proteins or DNA vaccine, in combination with rROP2 could be tested to try improving the efficacy of this kind of vaccine.Colégio Brasileiro de Parasitologia Veterinária2017-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612017000100067Revista Brasileira de Parasitologia Veterinária v.26 n.1 2017reponame:Revista Brasileira de Parasitologia Veterinária (Online)instname:Colégio Brasileiro de Parasitologia Veterinária (CBPV)instacron:CBPV10.1590/s1984-29612017007info:eu-repo/semantics/openAccessZulpo,Dauton LuizIgarashi,MichelleSammi,Ana SueSantos,Joeleni Rosa dosSasse,João PedroCunha,Ivo Alexandre Leme daTaroda,AlessandraBarros,Luiz Daniel deAlmeida,Jonatas Campos deJenkins,Mark ChristopherNavarro,Italmar TeodoricoGarcia,João Luiseng2017-04-04T00:00:00Zoai:scielo:S1984-29612017000100067Revistahttp://www.scielo.br/scielo.php?script=sci_serial&lng=pt&pid=1984-2961https://old.scielo.br/oai/scielo-oai.php||zacariascbpv@fcav.unesp.br1984-29610103-846Xopendoar:2017-04-04T00:00Revista Brasileira de Parasitologia Veterinária (Online) - Colégio Brasileiro de Parasitologia Veterinária (CBPV)false |
dc.title.none.fl_str_mv |
rROP2 from Toxoplasma gondii as a potential vaccine against oocyst shedding in domestic cats |
title |
rROP2 from Toxoplasma gondii as a potential vaccine against oocyst shedding in domestic cats |
spellingShingle |
rROP2 from Toxoplasma gondii as a potential vaccine against oocyst shedding in domestic cats Zulpo,Dauton Luiz Definitive host immunization recombinant protein toxoplasmosis |
title_short |
rROP2 from Toxoplasma gondii as a potential vaccine against oocyst shedding in domestic cats |
title_full |
rROP2 from Toxoplasma gondii as a potential vaccine against oocyst shedding in domestic cats |
title_fullStr |
rROP2 from Toxoplasma gondii as a potential vaccine against oocyst shedding in domestic cats |
title_full_unstemmed |
rROP2 from Toxoplasma gondii as a potential vaccine against oocyst shedding in domestic cats |
title_sort |
rROP2 from Toxoplasma gondii as a potential vaccine against oocyst shedding in domestic cats |
author |
Zulpo,Dauton Luiz |
author_facet |
Zulpo,Dauton Luiz Igarashi,Michelle Sammi,Ana Sue Santos,Joeleni Rosa dos Sasse,João Pedro Cunha,Ivo Alexandre Leme da Taroda,Alessandra Barros,Luiz Daniel de Almeida,Jonatas Campos de Jenkins,Mark Christopher Navarro,Italmar Teodorico Garcia,João Luis |
author_role |
author |
author2 |
Igarashi,Michelle Sammi,Ana Sue Santos,Joeleni Rosa dos Sasse,João Pedro Cunha,Ivo Alexandre Leme da Taroda,Alessandra Barros,Luiz Daniel de Almeida,Jonatas Campos de Jenkins,Mark Christopher Navarro,Italmar Teodorico Garcia,João Luis |
author2_role |
author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Zulpo,Dauton Luiz Igarashi,Michelle Sammi,Ana Sue Santos,Joeleni Rosa dos Sasse,João Pedro Cunha,Ivo Alexandre Leme da Taroda,Alessandra Barros,Luiz Daniel de Almeida,Jonatas Campos de Jenkins,Mark Christopher Navarro,Italmar Teodorico Garcia,João Luis |
dc.subject.por.fl_str_mv |
Definitive host immunization recombinant protein toxoplasmosis |
topic |
Definitive host immunization recombinant protein toxoplasmosis |
description |
Abstract The aim of the present study was to evaluate oocyst shedding in cats immunized by nasal route with T. gondii proteins ROP2. Twelve short hair cats (Felis catus) were divided in three groups G1, G2 and G3 (n=4). Animals from G1 received 100 μg of rROP2 proteins plus 20 μg of Quil-A, G2 received 100 μg of BSA plus 20 μg of Quil-A, and the G3 only saline solution (control group). All treatments were done by intranasal route at days 0, 21, 42, and 63. The challenge was performed in all groups on day 70 with ≅ 800 tissue cysts of ME-49 strain by oral route. Animals from G1 shed less oocysts (86.7%) than control groups. ELISA was used to detect anti-rROP2 IgG and IgA, however, there were no correlation between number of oocyst shedding by either IgG or IgA antibody levels. In the present work, in spite of lesser oocysts production in immunized group than control groups, it was not possible to associate the use of rROP2 via nostrils with protection against oocyst shedding. For the future, the use of either other recombinant proteins or DNA vaccine, in combination with rROP2 could be tested to try improving the efficacy of this kind of vaccine. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-03-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612017000100067 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612017000100067 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/s1984-29612017007 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Colégio Brasileiro de Parasitologia Veterinária |
publisher.none.fl_str_mv |
Colégio Brasileiro de Parasitologia Veterinária |
dc.source.none.fl_str_mv |
Revista Brasileira de Parasitologia Veterinária v.26 n.1 2017 reponame:Revista Brasileira de Parasitologia Veterinária (Online) instname:Colégio Brasileiro de Parasitologia Veterinária (CBPV) instacron:CBPV |
instname_str |
Colégio Brasileiro de Parasitologia Veterinária (CBPV) |
instacron_str |
CBPV |
institution |
CBPV |
reponame_str |
Revista Brasileira de Parasitologia Veterinária (Online) |
collection |
Revista Brasileira de Parasitologia Veterinária (Online) |
repository.name.fl_str_mv |
Revista Brasileira de Parasitologia Veterinária (Online) - Colégio Brasileiro de Parasitologia Veterinária (CBPV) |
repository.mail.fl_str_mv |
||zacariascbpv@fcav.unesp.br |
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1754208916609171456 |