rROP2 from Toxoplasma gondii as a potential vaccine against oocyst shedding in domestic cats

Detalhes bibliográficos
Autor(a) principal: Zulpo,Dauton Luiz
Data de Publicação: 2017
Outros Autores: Igarashi,Michelle, Sammi,Ana Sue, Santos,Joeleni Rosa dos, Sasse,João Pedro, Cunha,Ivo Alexandre Leme da, Taroda,Alessandra, Barros,Luiz Daniel de, Almeida,Jonatas Campos de, Jenkins,Mark Christopher, Navarro,Italmar Teodorico, Garcia,João Luis
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista Brasileira de Parasitologia Veterinária (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612017000100067
Resumo: Abstract The aim of the present study was to evaluate oocyst shedding in cats immunized by nasal route with T. gondii proteins ROP2. Twelve short hair cats (Felis catus) were divided in three groups G1, G2 and G3 (n=4). Animals from G1 received 100 μg of rROP2 proteins plus 20 μg of Quil-A, G2 received 100 μg of BSA plus 20 μg of Quil-A, and the G3 only saline solution (control group). All treatments were done by intranasal route at days 0, 21, 42, and 63. The challenge was performed in all groups on day 70 with ≅ 800 tissue cysts of ME-49 strain by oral route. Animals from G1 shed less oocysts (86.7%) than control groups. ELISA was used to detect anti-rROP2 IgG and IgA, however, there were no correlation between number of oocyst shedding by either IgG or IgA antibody levels. In the present work, in spite of lesser oocysts production in immunized group than control groups, it was not possible to associate the use of rROP2 via nostrils with protection against oocyst shedding. For the future, the use of either other recombinant proteins or DNA vaccine, in combination with rROP2 could be tested to try improving the efficacy of this kind of vaccine.
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spelling rROP2 from Toxoplasma gondii as a potential vaccine against oocyst shedding in domestic catsDefinitive hostimmunizationrecombinant proteintoxoplasmosisAbstract The aim of the present study was to evaluate oocyst shedding in cats immunized by nasal route with T. gondii proteins ROP2. Twelve short hair cats (Felis catus) were divided in three groups G1, G2 and G3 (n=4). Animals from G1 received 100 μg of rROP2 proteins plus 20 μg of Quil-A, G2 received 100 μg of BSA plus 20 μg of Quil-A, and the G3 only saline solution (control group). All treatments were done by intranasal route at days 0, 21, 42, and 63. The challenge was performed in all groups on day 70 with ≅ 800 tissue cysts of ME-49 strain by oral route. Animals from G1 shed less oocysts (86.7%) than control groups. ELISA was used to detect anti-rROP2 IgG and IgA, however, there were no correlation between number of oocyst shedding by either IgG or IgA antibody levels. In the present work, in spite of lesser oocysts production in immunized group than control groups, it was not possible to associate the use of rROP2 via nostrils with protection against oocyst shedding. For the future, the use of either other recombinant proteins or DNA vaccine, in combination with rROP2 could be tested to try improving the efficacy of this kind of vaccine.Colégio Brasileiro de Parasitologia Veterinária2017-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612017000100067Revista Brasileira de Parasitologia Veterinária v.26 n.1 2017reponame:Revista Brasileira de Parasitologia Veterinária (Online)instname:Colégio Brasileiro de Parasitologia Veterinária (CBPV)instacron:CBPV10.1590/s1984-29612017007info:eu-repo/semantics/openAccessZulpo,Dauton LuizIgarashi,MichelleSammi,Ana SueSantos,Joeleni Rosa dosSasse,João PedroCunha,Ivo Alexandre Leme daTaroda,AlessandraBarros,Luiz Daniel deAlmeida,Jonatas Campos deJenkins,Mark ChristopherNavarro,Italmar TeodoricoGarcia,João Luiseng2017-04-04T00:00:00Zoai:scielo:S1984-29612017000100067Revistahttp://www.scielo.br/scielo.php?script=sci_serial&lng=pt&pid=1984-2961https://old.scielo.br/oai/scielo-oai.php||zacariascbpv@fcav.unesp.br1984-29610103-846Xopendoar:2017-04-04T00:00Revista Brasileira de Parasitologia Veterinária (Online) - Colégio Brasileiro de Parasitologia Veterinária (CBPV)false
dc.title.none.fl_str_mv rROP2 from Toxoplasma gondii as a potential vaccine against oocyst shedding in domestic cats
title rROP2 from Toxoplasma gondii as a potential vaccine against oocyst shedding in domestic cats
spellingShingle rROP2 from Toxoplasma gondii as a potential vaccine against oocyst shedding in domestic cats
Zulpo,Dauton Luiz
Definitive host
immunization
recombinant protein
toxoplasmosis
title_short rROP2 from Toxoplasma gondii as a potential vaccine against oocyst shedding in domestic cats
title_full rROP2 from Toxoplasma gondii as a potential vaccine against oocyst shedding in domestic cats
title_fullStr rROP2 from Toxoplasma gondii as a potential vaccine against oocyst shedding in domestic cats
title_full_unstemmed rROP2 from Toxoplasma gondii as a potential vaccine against oocyst shedding in domestic cats
title_sort rROP2 from Toxoplasma gondii as a potential vaccine against oocyst shedding in domestic cats
author Zulpo,Dauton Luiz
author_facet Zulpo,Dauton Luiz
Igarashi,Michelle
Sammi,Ana Sue
Santos,Joeleni Rosa dos
Sasse,João Pedro
Cunha,Ivo Alexandre Leme da
Taroda,Alessandra
Barros,Luiz Daniel de
Almeida,Jonatas Campos de
Jenkins,Mark Christopher
Navarro,Italmar Teodorico
Garcia,João Luis
author_role author
author2 Igarashi,Michelle
Sammi,Ana Sue
Santos,Joeleni Rosa dos
Sasse,João Pedro
Cunha,Ivo Alexandre Leme da
Taroda,Alessandra
Barros,Luiz Daniel de
Almeida,Jonatas Campos de
Jenkins,Mark Christopher
Navarro,Italmar Teodorico
Garcia,João Luis
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Zulpo,Dauton Luiz
Igarashi,Michelle
Sammi,Ana Sue
Santos,Joeleni Rosa dos
Sasse,João Pedro
Cunha,Ivo Alexandre Leme da
Taroda,Alessandra
Barros,Luiz Daniel de
Almeida,Jonatas Campos de
Jenkins,Mark Christopher
Navarro,Italmar Teodorico
Garcia,João Luis
dc.subject.por.fl_str_mv Definitive host
immunization
recombinant protein
toxoplasmosis
topic Definitive host
immunization
recombinant protein
toxoplasmosis
description Abstract The aim of the present study was to evaluate oocyst shedding in cats immunized by nasal route with T. gondii proteins ROP2. Twelve short hair cats (Felis catus) were divided in three groups G1, G2 and G3 (n=4). Animals from G1 received 100 μg of rROP2 proteins plus 20 μg of Quil-A, G2 received 100 μg of BSA plus 20 μg of Quil-A, and the G3 only saline solution (control group). All treatments were done by intranasal route at days 0, 21, 42, and 63. The challenge was performed in all groups on day 70 with ≅ 800 tissue cysts of ME-49 strain by oral route. Animals from G1 shed less oocysts (86.7%) than control groups. ELISA was used to detect anti-rROP2 IgG and IgA, however, there were no correlation between number of oocyst shedding by either IgG or IgA antibody levels. In the present work, in spite of lesser oocysts production in immunized group than control groups, it was not possible to associate the use of rROP2 via nostrils with protection against oocyst shedding. For the future, the use of either other recombinant proteins or DNA vaccine, in combination with rROP2 could be tested to try improving the efficacy of this kind of vaccine.
publishDate 2017
dc.date.none.fl_str_mv 2017-03-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612017000100067
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612017000100067
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/s1984-29612017007
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Colégio Brasileiro de Parasitologia Veterinária
publisher.none.fl_str_mv Colégio Brasileiro de Parasitologia Veterinária
dc.source.none.fl_str_mv Revista Brasileira de Parasitologia Veterinária v.26 n.1 2017
reponame:Revista Brasileira de Parasitologia Veterinária (Online)
instname:Colégio Brasileiro de Parasitologia Veterinária (CBPV)
instacron:CBPV
instname_str Colégio Brasileiro de Parasitologia Veterinária (CBPV)
instacron_str CBPV
institution CBPV
reponame_str Revista Brasileira de Parasitologia Veterinária (Online)
collection Revista Brasileira de Parasitologia Veterinária (Online)
repository.name.fl_str_mv Revista Brasileira de Parasitologia Veterinária (Online) - Colégio Brasileiro de Parasitologia Veterinária (CBPV)
repository.mail.fl_str_mv ||zacariascbpv@fcav.unesp.br
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