Serology using rROP2 antigen in the diagnostic of toxoplasmosis in pregnant women

Detalhes bibliográficos
Autor(a) principal: Macre, Miriam de Souza
Data de Publicação: 2009
Outros Autores: Pires, Márcio, Meireles, Luciana Regina, Angel, Sérgio O., Andrade Jr., Heitor Franco de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista do Instituto de Medicina Tropical de São Paulo
Texto Completo: https://www.revistas.usp.br/rimtsp/article/view/31290
Resumo: Toxoplasma gondii causes severe fetal disease during acute infection in pregnant women, thus demanding early diagnosis for effective treatment and fetus preservation. Fetal tests are inefficient and risky, and diagnosis is based on maternal IgM serology, which had weak screening ability due to increased sensitivity, with alternative IgG avidity tests. Here, we performed ELISA and avidity assays using a recombinant T. gondii antigen, rROP2, in samples from 160 pregnant women screened from a large public hospital who were referred due to positive IgM assays. IgG serology and avidity assays were compared using whole T. gondii extract or rROP2. ELISA IgG detection with rROP2 showed good agreement with assays performed with T. gondii extract, but rROP2 IgG avidity assays were unrelated to whole extract antigen IgG avidity, regardless of the chaotrope used. These data show that avidity maturation is specific to individual antigen prevalence and immune response during infection. ELISA rROP2 IgG assays may be an alternative serological test for the diagnosis of toxoplasmosis during pregnancy, although our data do not support their use in avidity assays.
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spelling Serology using rROP2 antigen in the diagnostic of toxoplasmosis in pregnant women Sorologia utilizando o antígeno recombinante ROP2 no diagnóstico da toxoplasmose em mulheres grávidas ToxoplasmosisPregnancySerologyRecombinant proteinsAvidity Toxoplasma gondii causes severe fetal disease during acute infection in pregnant women, thus demanding early diagnosis for effective treatment and fetus preservation. Fetal tests are inefficient and risky, and diagnosis is based on maternal IgM serology, which had weak screening ability due to increased sensitivity, with alternative IgG avidity tests. Here, we performed ELISA and avidity assays using a recombinant T. gondii antigen, rROP2, in samples from 160 pregnant women screened from a large public hospital who were referred due to positive IgM assays. IgG serology and avidity assays were compared using whole T. gondii extract or rROP2. ELISA IgG detection with rROP2 showed good agreement with assays performed with T. gondii extract, but rROP2 IgG avidity assays were unrelated to whole extract antigen IgG avidity, regardless of the chaotrope used. These data show that avidity maturation is specific to individual antigen prevalence and immune response during infection. ELISA rROP2 IgG assays may be an alternative serological test for the diagnosis of toxoplasmosis during pregnancy, although our data do not support their use in avidity assays. A toxoplasmose causa grave doença fetal durante a infecção aguda da gestante, assim demanda diagnóstico precoce para tratamento efetivo e preservação fetal. Os testes fetais são de baixa eficiência e o diagnóstico é baseado na sorologia para IgM materna, que tem fraca capacidade de triagem devido a sensibilidade crescente dos testes, sendo uma alternativa os testes de avidez de IgG. Apresentamos aqui um ELISA e ensaio de avidez de IgG usando antígeno recombinante de T. gondii, rROP2, em amostras de 162 gestantes triadas para um grande hospital público por uma sorologia positiva para IgM. O ELISA IgG e o ensaio de avidez de IgG foram comparados usando o antígeno rROP2 ou extrato total de T. gondii. O ELISA usando rROP2 mostrou excelente concordância com os ensaios usando extrato de T. gondii, mas nos ensaios de avidez usando rROP2, os valores encontrados não se relacionaram com os encontrados na avidez com extrato de T. gondii, independente do tipo de caotrópico utilizado. Estes dados mostram que a maturação da avidez é especifica para a resposta imune ou a prevalência de um antígeno durante a infecção. Os ELISA para IgG anti rROP2 podem ser teste sorológico alternativo para o diagnóstico da toxoplasmose durante a gravidez, embora nossos dados não apóiem seu uso em ensaios de avidez de IgG Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo2009-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/31290Revista do Instituto de Medicina Tropical de São Paulo; Vol. 51 No. 5 (2009); 283-288 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 51 Núm. 5 (2009); 283-288 Revista do Instituto de Medicina Tropical de São Paulo; v. 51 n. 5 (2009); 283-288 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/31290/33175Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessMacre, Miriam de SouzaPires, MárcioMeireles, Luciana ReginaAngel, Sérgio O.Andrade Jr., Heitor Franco de2012-07-07T19:24:45Zoai:revistas.usp.br:article/31290Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:51:58.135209Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true
dc.title.none.fl_str_mv Serology using rROP2 antigen in the diagnostic of toxoplasmosis in pregnant women
Sorologia utilizando o antígeno recombinante ROP2 no diagnóstico da toxoplasmose em mulheres grávidas
title Serology using rROP2 antigen in the diagnostic of toxoplasmosis in pregnant women
spellingShingle Serology using rROP2 antigen in the diagnostic of toxoplasmosis in pregnant women
Macre, Miriam de Souza
Toxoplasmosis
Pregnancy
Serology
Recombinant proteins
Avidity
title_short Serology using rROP2 antigen in the diagnostic of toxoplasmosis in pregnant women
title_full Serology using rROP2 antigen in the diagnostic of toxoplasmosis in pregnant women
title_fullStr Serology using rROP2 antigen in the diagnostic of toxoplasmosis in pregnant women
title_full_unstemmed Serology using rROP2 antigen in the diagnostic of toxoplasmosis in pregnant women
title_sort Serology using rROP2 antigen in the diagnostic of toxoplasmosis in pregnant women
author Macre, Miriam de Souza
author_facet Macre, Miriam de Souza
Pires, Márcio
Meireles, Luciana Regina
Angel, Sérgio O.
Andrade Jr., Heitor Franco de
author_role author
author2 Pires, Márcio
Meireles, Luciana Regina
Angel, Sérgio O.
Andrade Jr., Heitor Franco de
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Macre, Miriam de Souza
Pires, Márcio
Meireles, Luciana Regina
Angel, Sérgio O.
Andrade Jr., Heitor Franco de
dc.subject.por.fl_str_mv Toxoplasmosis
Pregnancy
Serology
Recombinant proteins
Avidity
topic Toxoplasmosis
Pregnancy
Serology
Recombinant proteins
Avidity
description Toxoplasma gondii causes severe fetal disease during acute infection in pregnant women, thus demanding early diagnosis for effective treatment and fetus preservation. Fetal tests are inefficient and risky, and diagnosis is based on maternal IgM serology, which had weak screening ability due to increased sensitivity, with alternative IgG avidity tests. Here, we performed ELISA and avidity assays using a recombinant T. gondii antigen, rROP2, in samples from 160 pregnant women screened from a large public hospital who were referred due to positive IgM assays. IgG serology and avidity assays were compared using whole T. gondii extract or rROP2. ELISA IgG detection with rROP2 showed good agreement with assays performed with T. gondii extract, but rROP2 IgG avidity assays were unrelated to whole extract antigen IgG avidity, regardless of the chaotrope used. These data show that avidity maturation is specific to individual antigen prevalence and immune response during infection. ELISA rROP2 IgG assays may be an alternative serological test for the diagnosis of toxoplasmosis during pregnancy, although our data do not support their use in avidity assays.
publishDate 2009
dc.date.none.fl_str_mv 2009-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/31290
url https://www.revistas.usp.br/rimtsp/article/view/31290
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/31290/33175
dc.rights.driver.fl_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
dc.source.none.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo; Vol. 51 No. 5 (2009); 283-288
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 51 Núm. 5 (2009); 283-288
Revista do Instituto de Medicina Tropical de São Paulo; v. 51 n. 5 (2009); 283-288
1678-9946
0036-4665
reponame:Revista do Instituto de Medicina Tropical de São Paulo
instname:Instituto de Medicina Tropical (IMT)
instacron:IMT
instname_str Instituto de Medicina Tropical (IMT)
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reponame_str Revista do Instituto de Medicina Tropical de São Paulo
collection Revista do Instituto de Medicina Tropical de São Paulo
repository.name.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)
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