Morphological and molecular identification with frequency analysis of Calicophoron microbothrioides in central Chile
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista Brasileira de Parasitologia Veterinária (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612019000400582 |
Resumo: | Abstract This research aimed to determine the presence of paramphistomids in cattle slaughtered in a slaughterhouse of the Ñuble Region of Chile, to identify flukes and to analyze the frequency of these parasites in the Maule, Ñuble, and Biobío administrative regions of Chile. Between October of 2016 and April of 2017, rumens of 494 cattle were examined for flukes in the forestomachs. Worms were identified morphologically and, in addition, molecular analysis of the internal transcriber spacer region 2 of the fluke’s DNA was done and phylogenetic analyses were performed with Bayesian inference in 14 worms. The frequency was analyzed by locality (low- or highlands) and age. The overall frequency was 11.24%. The district with the highest frequency of presentation was Chillán Viejo (30.8%). Districts in the lowlands had similar frequencies to those in the mountain lands (p=0.1). The frequency of flukes was significantly higher in adult animals than in young ones (p<0.01). We obtained a 460 bp-length fragment of DNA that was identical to the sequences previously identified as Paramphistomum cervi and Calicophoron microbothrioides, and performed morphological analyses confirmed that our samples belonged to C. microbothrioides. This is the first published study of C. microbothrioides in Chile. |
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Morphological and molecular identification with frequency analysis of Calicophoron microbothrioides in central ChileParamphistomidaeruminal flukesbovineepidemiologyITS-2Calicophoron microbothrioidesAbstract This research aimed to determine the presence of paramphistomids in cattle slaughtered in a slaughterhouse of the Ñuble Region of Chile, to identify flukes and to analyze the frequency of these parasites in the Maule, Ñuble, and Biobío administrative regions of Chile. Between October of 2016 and April of 2017, rumens of 494 cattle were examined for flukes in the forestomachs. Worms were identified morphologically and, in addition, molecular analysis of the internal transcriber spacer region 2 of the fluke’s DNA was done and phylogenetic analyses were performed with Bayesian inference in 14 worms. The frequency was analyzed by locality (low- or highlands) and age. The overall frequency was 11.24%. The district with the highest frequency of presentation was Chillán Viejo (30.8%). Districts in the lowlands had similar frequencies to those in the mountain lands (p=0.1). The frequency of flukes was significantly higher in adult animals than in young ones (p<0.01). We obtained a 460 bp-length fragment of DNA that was identical to the sequences previously identified as Paramphistomum cervi and Calicophoron microbothrioides, and performed morphological analyses confirmed that our samples belonged to C. microbothrioides. This is the first published study of C. microbothrioides in Chile.Colégio Brasileiro de Parasitologia Veterinária2019-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612019000400582Revista Brasileira de Parasitologia Veterinária v.28 n.4 2019reponame:Revista Brasileira de Parasitologia Veterinária (Online)instname:Colégio Brasileiro de Parasitologia Veterinária (CBPV)instacron:CBPV10.1590/s1984-29612019076info:eu-repo/semantics/openAccessCerda,CarolinaVeloso-Frías,JoselineLobos-Chávez,FelipeOyarzún-Ruiz,PabloHenríquez,AnaLíaLoyola,MarcoFuente,María Carolina Silva-de laOrtega,RenéLetelier,ReinaldoLandaeta-Aqueveque,Carloseng2020-04-14T00:00:00Zoai:scielo:S1984-29612019000400582Revistahttp://www.scielo.br/scielo.php?script=sci_serial&lng=pt&pid=1984-2961https://old.scielo.br/oai/scielo-oai.php||zacariascbpv@fcav.unesp.br1984-29610103-846Xopendoar:2020-04-14T00:00Revista Brasileira de Parasitologia Veterinária (Online) - Colégio Brasileiro de Parasitologia Veterinária (CBPV)false |
dc.title.none.fl_str_mv |
Morphological and molecular identification with frequency analysis of Calicophoron microbothrioides in central Chile |
title |
Morphological and molecular identification with frequency analysis of Calicophoron microbothrioides in central Chile |
spellingShingle |
Morphological and molecular identification with frequency analysis of Calicophoron microbothrioides in central Chile Cerda,Carolina Paramphistomidae ruminal flukes bovine epidemiology ITS-2 Calicophoron microbothrioides |
title_short |
Morphological and molecular identification with frequency analysis of Calicophoron microbothrioides in central Chile |
title_full |
Morphological and molecular identification with frequency analysis of Calicophoron microbothrioides in central Chile |
title_fullStr |
Morphological and molecular identification with frequency analysis of Calicophoron microbothrioides in central Chile |
title_full_unstemmed |
Morphological and molecular identification with frequency analysis of Calicophoron microbothrioides in central Chile |
title_sort |
Morphological and molecular identification with frequency analysis of Calicophoron microbothrioides in central Chile |
author |
Cerda,Carolina |
author_facet |
Cerda,Carolina Veloso-Frías,Joseline Lobos-Chávez,Felipe Oyarzún-Ruiz,Pablo Henríquez,AnaLía Loyola,Marco Fuente,María Carolina Silva-de la Ortega,René Letelier,Reinaldo Landaeta-Aqueveque,Carlos |
author_role |
author |
author2 |
Veloso-Frías,Joseline Lobos-Chávez,Felipe Oyarzún-Ruiz,Pablo Henríquez,AnaLía Loyola,Marco Fuente,María Carolina Silva-de la Ortega,René Letelier,Reinaldo Landaeta-Aqueveque,Carlos |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Cerda,Carolina Veloso-Frías,Joseline Lobos-Chávez,Felipe Oyarzún-Ruiz,Pablo Henríquez,AnaLía Loyola,Marco Fuente,María Carolina Silva-de la Ortega,René Letelier,Reinaldo Landaeta-Aqueveque,Carlos |
dc.subject.por.fl_str_mv |
Paramphistomidae ruminal flukes bovine epidemiology ITS-2 Calicophoron microbothrioides |
topic |
Paramphistomidae ruminal flukes bovine epidemiology ITS-2 Calicophoron microbothrioides |
description |
Abstract This research aimed to determine the presence of paramphistomids in cattle slaughtered in a slaughterhouse of the Ñuble Region of Chile, to identify flukes and to analyze the frequency of these parasites in the Maule, Ñuble, and Biobío administrative regions of Chile. Between October of 2016 and April of 2017, rumens of 494 cattle were examined for flukes in the forestomachs. Worms were identified morphologically and, in addition, molecular analysis of the internal transcriber spacer region 2 of the fluke’s DNA was done and phylogenetic analyses were performed with Bayesian inference in 14 worms. The frequency was analyzed by locality (low- or highlands) and age. The overall frequency was 11.24%. The district with the highest frequency of presentation was Chillán Viejo (30.8%). Districts in the lowlands had similar frequencies to those in the mountain lands (p=0.1). The frequency of flukes was significantly higher in adult animals than in young ones (p<0.01). We obtained a 460 bp-length fragment of DNA that was identical to the sequences previously identified as Paramphistomum cervi and Calicophoron microbothrioides, and performed morphological analyses confirmed that our samples belonged to C. microbothrioides. This is the first published study of C. microbothrioides in Chile. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612019000400582 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612019000400582 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/s1984-29612019076 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Colégio Brasileiro de Parasitologia Veterinária |
publisher.none.fl_str_mv |
Colégio Brasileiro de Parasitologia Veterinária |
dc.source.none.fl_str_mv |
Revista Brasileira de Parasitologia Veterinária v.28 n.4 2019 reponame:Revista Brasileira de Parasitologia Veterinária (Online) instname:Colégio Brasileiro de Parasitologia Veterinária (CBPV) instacron:CBPV |
instname_str |
Colégio Brasileiro de Parasitologia Veterinária (CBPV) |
instacron_str |
CBPV |
institution |
CBPV |
reponame_str |
Revista Brasileira de Parasitologia Veterinária (Online) |
collection |
Revista Brasileira de Parasitologia Veterinária (Online) |
repository.name.fl_str_mv |
Revista Brasileira de Parasitologia Veterinária (Online) - Colégio Brasileiro de Parasitologia Veterinária (CBPV) |
repository.mail.fl_str_mv |
||zacariascbpv@fcav.unesp.br |
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1754208918393847808 |