Diagnosis of Neospora caninum in bovine fetuses by histology, immunohistochemistry, and nested-PCR
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista Brasileira de Parasitologia Veterinária (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612009000400003 |
Resumo: | Neospora caninum, a cause of abortion and stillbirth in cattle, was studied by histology, immunohistochemistry, and nested-PCR, using primers from the Nc5 region of the genomic DNA (PCR PLUS) and primers from the ITS1 region of the ribosomal DNA (PCR JB). A total of 105 fetal samples sent to the Centro de Pesquisa e Desenvolvimento de Sanidade Animal do Instituto Biológico from January 2006 to May 2008 were examined for evidence of N. caninum. Histological examination revealed 71.4% with non-suppurative inflammation in the heart, lung, liver, kidney, placenta, and brain. Immunohistochemistry detected infections in 8.6% of the samples, mainly in the brain, placenta, and heart. Nested-PCR JB revealed 6.7% with infections, while nested-PCR PLUS returned 20.9% positive results, mainly in brain and placenta, and in the pooled liver and heart. Kappa statistics demonstrated little agreement among the three techniques. The three methods are complementary, since they have distinct diagnostic characteristics and were combined to give a positivity rate of 24.8%. |
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Diagnosis of Neospora caninum in bovine fetuses by histology, immunohistochemistry, and nested-PCRNeosporosisabortioncattleBrazildiagnosisNeospora caninum, a cause of abortion and stillbirth in cattle, was studied by histology, immunohistochemistry, and nested-PCR, using primers from the Nc5 region of the genomic DNA (PCR PLUS) and primers from the ITS1 region of the ribosomal DNA (PCR JB). A total of 105 fetal samples sent to the Centro de Pesquisa e Desenvolvimento de Sanidade Animal do Instituto Biológico from January 2006 to May 2008 were examined for evidence of N. caninum. Histological examination revealed 71.4% with non-suppurative inflammation in the heart, lung, liver, kidney, placenta, and brain. Immunohistochemistry detected infections in 8.6% of the samples, mainly in the brain, placenta, and heart. Nested-PCR JB revealed 6.7% with infections, while nested-PCR PLUS returned 20.9% positive results, mainly in brain and placenta, and in the pooled liver and heart. Kappa statistics demonstrated little agreement among the three techniques. The three methods are complementary, since they have distinct diagnostic characteristics and were combined to give a positivity rate of 24.8%.Colégio Brasileiro de Parasitologia Veterinária2009-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612009000400003Revista Brasileira de Parasitologia Veterinária v.18 n.4 2009reponame:Revista Brasileira de Parasitologia Veterinária (Online)instname:Colégio Brasileiro de Parasitologia Veterinária (CBPV)instacron:CBPV10.4322/rbpv.01804003info:eu-repo/semantics/openAccessCabral,Aline DinizCamargo,Clarice NevesGalleti,Nara Thiers CacciatoriOkuda,Liria HiromiPituco,Edviges MaristelaDel Fava,Claudiaeng2012-04-12T00:00:00Zoai:scielo:S1984-29612009000400003Revistahttp://www.scielo.br/scielo.php?script=sci_serial&lng=pt&pid=1984-2961https://old.scielo.br/oai/scielo-oai.php||zacariascbpv@fcav.unesp.br1984-29610103-846Xopendoar:2012-04-12T00:00Revista Brasileira de Parasitologia Veterinária (Online) - Colégio Brasileiro de Parasitologia Veterinária (CBPV)false |
dc.title.none.fl_str_mv |
Diagnosis of Neospora caninum in bovine fetuses by histology, immunohistochemistry, and nested-PCR |
title |
Diagnosis of Neospora caninum in bovine fetuses by histology, immunohistochemistry, and nested-PCR |
spellingShingle |
Diagnosis of Neospora caninum in bovine fetuses by histology, immunohistochemistry, and nested-PCR Cabral,Aline Diniz Neosporosis abortion cattle Brazil diagnosis |
title_short |
Diagnosis of Neospora caninum in bovine fetuses by histology, immunohistochemistry, and nested-PCR |
title_full |
Diagnosis of Neospora caninum in bovine fetuses by histology, immunohistochemistry, and nested-PCR |
title_fullStr |
Diagnosis of Neospora caninum in bovine fetuses by histology, immunohistochemistry, and nested-PCR |
title_full_unstemmed |
Diagnosis of Neospora caninum in bovine fetuses by histology, immunohistochemistry, and nested-PCR |
title_sort |
Diagnosis of Neospora caninum in bovine fetuses by histology, immunohistochemistry, and nested-PCR |
author |
Cabral,Aline Diniz |
author_facet |
Cabral,Aline Diniz Camargo,Clarice Neves Galleti,Nara Thiers Cacciatori Okuda,Liria Hiromi Pituco,Edviges Maristela Del Fava,Claudia |
author_role |
author |
author2 |
Camargo,Clarice Neves Galleti,Nara Thiers Cacciatori Okuda,Liria Hiromi Pituco,Edviges Maristela Del Fava,Claudia |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Cabral,Aline Diniz Camargo,Clarice Neves Galleti,Nara Thiers Cacciatori Okuda,Liria Hiromi Pituco,Edviges Maristela Del Fava,Claudia |
dc.subject.por.fl_str_mv |
Neosporosis abortion cattle Brazil diagnosis |
topic |
Neosporosis abortion cattle Brazil diagnosis |
description |
Neospora caninum, a cause of abortion and stillbirth in cattle, was studied by histology, immunohistochemistry, and nested-PCR, using primers from the Nc5 region of the genomic DNA (PCR PLUS) and primers from the ITS1 region of the ribosomal DNA (PCR JB). A total of 105 fetal samples sent to the Centro de Pesquisa e Desenvolvimento de Sanidade Animal do Instituto Biológico from January 2006 to May 2008 were examined for evidence of N. caninum. Histological examination revealed 71.4% with non-suppurative inflammation in the heart, lung, liver, kidney, placenta, and brain. Immunohistochemistry detected infections in 8.6% of the samples, mainly in the brain, placenta, and heart. Nested-PCR JB revealed 6.7% with infections, while nested-PCR PLUS returned 20.9% positive results, mainly in brain and placenta, and in the pooled liver and heart. Kappa statistics demonstrated little agreement among the three techniques. The three methods are complementary, since they have distinct diagnostic characteristics and were combined to give a positivity rate of 24.8%. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612009000400003 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612009000400003 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.4322/rbpv.01804003 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Colégio Brasileiro de Parasitologia Veterinária |
publisher.none.fl_str_mv |
Colégio Brasileiro de Parasitologia Veterinária |
dc.source.none.fl_str_mv |
Revista Brasileira de Parasitologia Veterinária v.18 n.4 2009 reponame:Revista Brasileira de Parasitologia Veterinária (Online) instname:Colégio Brasileiro de Parasitologia Veterinária (CBPV) instacron:CBPV |
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Colégio Brasileiro de Parasitologia Veterinária (CBPV) |
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CBPV |
institution |
CBPV |
reponame_str |
Revista Brasileira de Parasitologia Veterinária (Online) |
collection |
Revista Brasileira de Parasitologia Veterinária (Online) |
repository.name.fl_str_mv |
Revista Brasileira de Parasitologia Veterinária (Online) - Colégio Brasileiro de Parasitologia Veterinária (CBPV) |
repository.mail.fl_str_mv |
||zacariascbpv@fcav.unesp.br |
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1754208913538940928 |