Combination of monoclonal antibodies improves immunohistochemical diagnosis of Neospora caninum

Detalhes bibliográficos
Autor(a) principal: Uzêda, Rosângela Soares
Data de Publicação: 2013
Outros Autores: Schares, Gereon, Ortega Mora, L. M., Madruga, C. R., Martinez, A. Aguado, Corbellini, Luís Gustavo, Driemeier, David, Gondim, Luis Fernando Pita
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://repositorio.ufba.br/ri/handle/ri/14851
Resumo: Texto completo: acesso restrito. p. 477–486
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spelling Uzêda, Rosângela SoaresSchares, GereonOrtega Mora, L. M.Madruga, C. R.Martinez, A. AguadoCorbellini, Luís GustavoDriemeier, DavidGondim, Luis Fernando PitaUzêda, Rosângela SoaresSchares, GereonOrtega Mora, L. M.Madruga, C. R.Martinez, A. AguadoCorbellini, Luís GustavoDriemeier, DavidGondim, Luis Fernando Pita2014-04-22T18:48:39Z20130304-4017http://repositorio.ufba.br/ri/handle/ri/14851v. 197, n. 3-4Texto completo: acesso restrito. p. 477–486Histological analysis is commonly used for a conclusive diagnosis of neosporosis. Immunohistochemistry (IHC) using monoclonal (mAb) and polyclonal (pAb) antibodies can improve diagnosis; however, the use of pAb may induce cross-reactivity with other related parasites. The aims of this study were to compare the performance of mAbs and their combinations with that of pAb in IHC and evaluate the usefulness of mAb to identify Neospora caninum infection in aborted bovine fetal tissues. For this purpose, mAbs targeting NcSRS2 (4.15.15) or NcGRA7 (4.11.5 and 1/24-12) and one pAb collected from a rabbit inoculated with N. caninum tachyzoites were tested by IHC. Artificial standardized tissue sections were prepared as positive controls using homogenized bovine brain spiked with cultured tachyzoites of N. caninum. The numbers of labeled parasites were counted in each positive control section. In addition, four equal proportional combinations of the mAbs were also analyzed in the IHC. Finally, the pAb and the best combination of mAbs obtained in the positive control experiments were tested with tissue sections of naturally-infected cattle. To confirm analytical specificity, mAbs and a pAb were tested with Toxoplasma gondii and Besnoitia besnoiti positive control slides and tissues sections from naturally infected cattle containing Sarcocystis spp. and B. besnoiti antigens. The mAb 4.15.15 detected 57% of the total parasites in sections while 4.11.5 and 1/24-12 were able to detect 49% and 41%, respectively. For the mAb combinations (I: 1/24-12 + 4.11.5, II: 1/24-12 + 4.15.15, III: 4.15.15 + 4.11.5, IV: 1/24-12 + 4.11.5 + 4.15.15), the detection capacity was 32.4%, 79.4%, 66.6% and 60.7% for each combination, respectively. The best mAb combination (1/24-12 and 4.15.15) and the pAb serum detected 100% (18/18) of naturally-infected animals. Sarcocystis spp. or B. besnoiti were not detected by mAb combinations in IHC, however the pAb cross-reacted with Sarcocystis spp. cysts. These results confirm the usefulness of mAb application in IHC to N. caninum.Submitted by Suelen Reis (suziy.ellen@gmail.com) on 2014-04-22T17:07:17Z No. of bitstreams: 1 1-s2.0-S0304401713003828-main.pdf: 2184409 bytes, checksum: f39601aacf966553b05d15852690ed7c (MD5)Approved for entry into archive by Alda Lima da Silva (sivalda@ufba.br) on 2014-04-22T18:48:39Z (GMT) No. of bitstreams: 1 1-s2.0-S0304401713003828-main.pdf: 2184409 bytes, checksum: f39601aacf966553b05d15852690ed7c (MD5)Made available in DSpace on 2014-04-22T18:48:39Z (GMT). No. of bitstreams: 1 1-s2.0-S0304401713003828-main.pdf: 2184409 bytes, checksum: f39601aacf966553b05d15852690ed7c (MD5) Previous issue date: 2013http://dx.doi.org/10.1016/j.vetpar.2013.07.008reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBANeosporosisMonoclonal antibodiesImmunohistochemistryAbortionCattleCombination of monoclonal antibodies improves immunohistochemical diagnosis of Neospora caninumVeterinary Parasitologyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10000-01-01info:eu-repo/semantics/openAccessengORIGINAL1-s2.0-S0304401713003828-main.pdf1-s2.0-S0304401713003828-main.pdfapplication/pdf2184409https://repositorio.ufba.br/bitstream/ri/14851/1/1-s2.0-S0304401713003828-main.pdff39601aacf966553b05d15852690ed7cMD51LICENSElicense.txtlicense.txttext/plain1345https://repositorio.ufba.br/bitstream/ri/14851/2/license.txt0d4b811ef71182510d2015daa7c8a900MD52TEXT1-s2.0-S0304401713003828-main.pdf.txt1-s2.0-S0304401713003828-main.pdf.txtExtracted texttext/plain47182https://repositorio.ufba.br/bitstream/ri/14851/3/1-s2.0-S0304401713003828-main.pdf.txt08ceacb932983b7a471d8b6b4815ed87MD53ri/148512022-07-05 14:03:42.266oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:03:42Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv Combination of monoclonal antibodies improves immunohistochemical diagnosis of Neospora caninum
dc.title.alternative.pt_BR.fl_str_mv Veterinary Parasitology
title Combination of monoclonal antibodies improves immunohistochemical diagnosis of Neospora caninum
spellingShingle Combination of monoclonal antibodies improves immunohistochemical diagnosis of Neospora caninum
Uzêda, Rosângela Soares
Neosporosis
Monoclonal antibodies
Immunohistochemistry
Abortion
Cattle
title_short Combination of monoclonal antibodies improves immunohistochemical diagnosis of Neospora caninum
title_full Combination of monoclonal antibodies improves immunohistochemical diagnosis of Neospora caninum
title_fullStr Combination of monoclonal antibodies improves immunohistochemical diagnosis of Neospora caninum
title_full_unstemmed Combination of monoclonal antibodies improves immunohistochemical diagnosis of Neospora caninum
title_sort Combination of monoclonal antibodies improves immunohistochemical diagnosis of Neospora caninum
author Uzêda, Rosângela Soares
author_facet Uzêda, Rosângela Soares
Schares, Gereon
Ortega Mora, L. M.
Madruga, C. R.
Martinez, A. Aguado
Corbellini, Luís Gustavo
Driemeier, David
Gondim, Luis Fernando Pita
author_role author
author2 Schares, Gereon
Ortega Mora, L. M.
Madruga, C. R.
Martinez, A. Aguado
Corbellini, Luís Gustavo
Driemeier, David
Gondim, Luis Fernando Pita
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Uzêda, Rosângela Soares
Schares, Gereon
Ortega Mora, L. M.
Madruga, C. R.
Martinez, A. Aguado
Corbellini, Luís Gustavo
Driemeier, David
Gondim, Luis Fernando Pita
Uzêda, Rosângela Soares
Schares, Gereon
Ortega Mora, L. M.
Madruga, C. R.
Martinez, A. Aguado
Corbellini, Luís Gustavo
Driemeier, David
Gondim, Luis Fernando Pita
dc.subject.por.fl_str_mv Neosporosis
Monoclonal antibodies
Immunohistochemistry
Abortion
Cattle
topic Neosporosis
Monoclonal antibodies
Immunohistochemistry
Abortion
Cattle
description Texto completo: acesso restrito. p. 477–486
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2014-04-22T18:48:39Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://repositorio.ufba.br/ri/handle/ri/14851
dc.identifier.issn.none.fl_str_mv 0304-4017
dc.identifier.number.pt_BR.fl_str_mv v. 197, n. 3-4
identifier_str_mv 0304-4017
v. 197, n. 3-4
url http://repositorio.ufba.br/ri/handle/ri/14851
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.pt_BR.fl_str_mv http://dx.doi.org/10.1016/j.vetpar.2013.07.008
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFBA
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instname_str Universidade Federal da Bahia (UFBA)
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reponame_str Repositório Institucional da UFBA
collection Repositório Institucional da UFBA
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