Diagnosis and isolation of Toxoplasma gondii in horses from Brazilian slaughterhouses
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista Brasileira de Parasitologia Veterinária (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612013000100058 |
Resumo: | This study aimed to investigate anti-Toxoplasma gondii antibodies and to isolate the parasite from the brains of horses processed at slaughterhouses in Brazil. We collected brain and blood samples from 398 horses of various ages, from six Brazilian states. Serum samples were evaluated by indirect fluorescent antibody test (IFAT cut-off titre ≥ 1:64), and brains were submitted to mouse bioassay. Among the 398 horses, positivity for T. gondii was identified in 46 (11.6%) by IFAT and in 14 (3.5%) by mouse bioassay. In 12 of those 14 bioassays, mice were positive only by IFAT (cut-off titre ≥ 1:16), T. gondii being isolated in the remaining two. Using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of 18S rDNA to differentiate among T. gondii, Neospora caninum, and Sarcocystis neurona, we found that two of the 14 brains were positive for T. gondii only. For genotyping of the two isolates and the PCR-positive brain, we performed PCR-RFLP based on 13 markers, and SAG2 all samples were Toxoplasma gondii type I. Collectively, IFAT of horse sera and mouse bioassay identified positivity in 60 (15%) of the samples. Our results show that some horses sent to slaughter in Brazil have been exposed toT. gondii. |
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Diagnosis and isolation of Toxoplasma gondii in horses from Brazilian slaughterhousesSlaughterhousehorsesToxoplasma gondiibioassayindirect fluorescent antibody testnested PCR-RFLPThis study aimed to investigate anti-Toxoplasma gondii antibodies and to isolate the parasite from the brains of horses processed at slaughterhouses in Brazil. We collected brain and blood samples from 398 horses of various ages, from six Brazilian states. Serum samples were evaluated by indirect fluorescent antibody test (IFAT cut-off titre ≥ 1:64), and brains were submitted to mouse bioassay. Among the 398 horses, positivity for T. gondii was identified in 46 (11.6%) by IFAT and in 14 (3.5%) by mouse bioassay. In 12 of those 14 bioassays, mice were positive only by IFAT (cut-off titre ≥ 1:16), T. gondii being isolated in the remaining two. Using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of 18S rDNA to differentiate among T. gondii, Neospora caninum, and Sarcocystis neurona, we found that two of the 14 brains were positive for T. gondii only. For genotyping of the two isolates and the PCR-positive brain, we performed PCR-RFLP based on 13 markers, and SAG2 all samples were Toxoplasma gondii type I. Collectively, IFAT of horse sera and mouse bioassay identified positivity in 60 (15%) of the samples. Our results show that some horses sent to slaughter in Brazil have been exposed toT. gondii.Colégio Brasileiro de Parasitologia Veterinária2013-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612013000100058Revista Brasileira de Parasitologia Veterinária v.22 n.1 2013reponame:Revista Brasileira de Parasitologia Veterinária (Online)instname:Colégio Brasileiro de Parasitologia Veterinária (CBPV)instacron:CBPV10.1590/S1984-29612013005000009info:eu-repo/semantics/openAccessEvers,FernandaGarcia,João LuisNavarro,Italmar TeodoricoZulpo,Dauton LuizNino,Beatriz de Souza LimaEwald,Maria Paula de CarvalhoPagliari,SthefanyAlmeida,Jonatas Campos deFreire,Roberta Lemoseng2015-11-24T00:00:00Zoai:scielo:S1984-29612013000100058Revistahttp://www.scielo.br/scielo.php?script=sci_serial&lng=pt&pid=1984-2961https://old.scielo.br/oai/scielo-oai.php||zacariascbpv@fcav.unesp.br1984-29610103-846Xopendoar:2015-11-24T00:00Revista Brasileira de Parasitologia Veterinária (Online) - Colégio Brasileiro de Parasitologia Veterinária (CBPV)false |
dc.title.none.fl_str_mv |
Diagnosis and isolation of Toxoplasma gondii in horses from Brazilian slaughterhouses |
title |
Diagnosis and isolation of Toxoplasma gondii in horses from Brazilian slaughterhouses |
spellingShingle |
Diagnosis and isolation of Toxoplasma gondii in horses from Brazilian slaughterhouses Evers,Fernanda Slaughterhouse horses Toxoplasma gondii bioassay indirect fluorescent antibody test nested PCR-RFLP |
title_short |
Diagnosis and isolation of Toxoplasma gondii in horses from Brazilian slaughterhouses |
title_full |
Diagnosis and isolation of Toxoplasma gondii in horses from Brazilian slaughterhouses |
title_fullStr |
Diagnosis and isolation of Toxoplasma gondii in horses from Brazilian slaughterhouses |
title_full_unstemmed |
Diagnosis and isolation of Toxoplasma gondii in horses from Brazilian slaughterhouses |
title_sort |
Diagnosis and isolation of Toxoplasma gondii in horses from Brazilian slaughterhouses |
author |
Evers,Fernanda |
author_facet |
Evers,Fernanda Garcia,João Luis Navarro,Italmar Teodorico Zulpo,Dauton Luiz Nino,Beatriz de Souza Lima Ewald,Maria Paula de Carvalho Pagliari,Sthefany Almeida,Jonatas Campos de Freire,Roberta Lemos |
author_role |
author |
author2 |
Garcia,João Luis Navarro,Italmar Teodorico Zulpo,Dauton Luiz Nino,Beatriz de Souza Lima Ewald,Maria Paula de Carvalho Pagliari,Sthefany Almeida,Jonatas Campos de Freire,Roberta Lemos |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Evers,Fernanda Garcia,João Luis Navarro,Italmar Teodorico Zulpo,Dauton Luiz Nino,Beatriz de Souza Lima Ewald,Maria Paula de Carvalho Pagliari,Sthefany Almeida,Jonatas Campos de Freire,Roberta Lemos |
dc.subject.por.fl_str_mv |
Slaughterhouse horses Toxoplasma gondii bioassay indirect fluorescent antibody test nested PCR-RFLP |
topic |
Slaughterhouse horses Toxoplasma gondii bioassay indirect fluorescent antibody test nested PCR-RFLP |
description |
This study aimed to investigate anti-Toxoplasma gondii antibodies and to isolate the parasite from the brains of horses processed at slaughterhouses in Brazil. We collected brain and blood samples from 398 horses of various ages, from six Brazilian states. Serum samples were evaluated by indirect fluorescent antibody test (IFAT cut-off titre ≥ 1:64), and brains were submitted to mouse bioassay. Among the 398 horses, positivity for T. gondii was identified in 46 (11.6%) by IFAT and in 14 (3.5%) by mouse bioassay. In 12 of those 14 bioassays, mice were positive only by IFAT (cut-off titre ≥ 1:16), T. gondii being isolated in the remaining two. Using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of 18S rDNA to differentiate among T. gondii, Neospora caninum, and Sarcocystis neurona, we found that two of the 14 brains were positive for T. gondii only. For genotyping of the two isolates and the PCR-positive brain, we performed PCR-RFLP based on 13 markers, and SAG2 all samples were Toxoplasma gondii type I. Collectively, IFAT of horse sera and mouse bioassay identified positivity in 60 (15%) of the samples. Our results show that some horses sent to slaughter in Brazil have been exposed toT. gondii. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-03-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612013000100058 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612013000100058 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1984-29612013005000009 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Colégio Brasileiro de Parasitologia Veterinária |
publisher.none.fl_str_mv |
Colégio Brasileiro de Parasitologia Veterinária |
dc.source.none.fl_str_mv |
Revista Brasileira de Parasitologia Veterinária v.22 n.1 2013 reponame:Revista Brasileira de Parasitologia Veterinária (Online) instname:Colégio Brasileiro de Parasitologia Veterinária (CBPV) instacron:CBPV |
instname_str |
Colégio Brasileiro de Parasitologia Veterinária (CBPV) |
instacron_str |
CBPV |
institution |
CBPV |
reponame_str |
Revista Brasileira de Parasitologia Veterinária (Online) |
collection |
Revista Brasileira de Parasitologia Veterinária (Online) |
repository.name.fl_str_mv |
Revista Brasileira de Parasitologia Veterinária (Online) - Colégio Brasileiro de Parasitologia Veterinária (CBPV) |
repository.mail.fl_str_mv |
||zacariascbpv@fcav.unesp.br |
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1754208914476367872 |