Toxoplasma gondii: diagnosis of experimental and natural infection in pigeons (Columba livia) by serological, biological and molecular techniques

Detalhes bibliográficos
Autor(a) principal: Godoi,Fernanda Sartori Lima de
Data de Publicação: 2010
Outros Autores: Nishi,Sandra Mayumi, Pena,Hilda Fátima de Jesus, Gennari,Solange Maria
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista Brasileira de Parasitologia Veterinária (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612010000400009
Resumo: This study aimed to diagnose experimental and natural Toxoplasma gondii infection in pigeons (Columba livia) by serological, biological and molecular techniques. Twelve pigeons, free of infection, were inoculated with 50 sporulated oocysts of T. gondii (VEG sample) and four remained uninfected controls. Four birds (three infected and one control) were euthanized at 15, 30, 45 and 60 days post-infection (dpi), and their tissues were used to perform a bioassay in mice and nested-PCR using B1 gene as target. Blood was obtained weekly and it was tested for the presence of anti-T. gondii antibodies by the indirect fluorescent antibody test (IFAT) and modified agglutination test (MAT). Seven (58.3%) out of 12 inoculated pigeons were positive by serological techniques and titers ranged between 1:40 and 1:5120 by MAT and between 1:512 and 1:4096 by IFAT. Complete agreement was seen between the results obtained by serological techniques and nested-PCR in seven positive birds. In the bioassay in mice, five (41.7%) out of 12 pigeons inoculated were positive to T. gondii. Only one pigeon died at 23 dpi due to toxoplasmosis. A second study with free-living pigeons was performed for detection of anti-T. gondii antibodies. Birds were captured in the municipalities of São Paulo, Ibiúna and Sorocaba, São Paulo State, Southeastern Brazil. All 126 free-living birds were negative to anti-T. gondii antibodies by MAT (titer < 1:5). Bioassays were performed in mice with tissues from all captured birds and T. gondii was not isolated in any pigeon.
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spelling Toxoplasma gondii: diagnosis of experimental and natural infection in pigeons (Columba livia) by serological, biological and molecular techniquesToxoplasma gondiipigeonsexperimental infectionbioassaynested-PCRThis study aimed to diagnose experimental and natural Toxoplasma gondii infection in pigeons (Columba livia) by serological, biological and molecular techniques. Twelve pigeons, free of infection, were inoculated with 50 sporulated oocysts of T. gondii (VEG sample) and four remained uninfected controls. Four birds (three infected and one control) were euthanized at 15, 30, 45 and 60 days post-infection (dpi), and their tissues were used to perform a bioassay in mice and nested-PCR using B1 gene as target. Blood was obtained weekly and it was tested for the presence of anti-T. gondii antibodies by the indirect fluorescent antibody test (IFAT) and modified agglutination test (MAT). Seven (58.3%) out of 12 inoculated pigeons were positive by serological techniques and titers ranged between 1:40 and 1:5120 by MAT and between 1:512 and 1:4096 by IFAT. Complete agreement was seen between the results obtained by serological techniques and nested-PCR in seven positive birds. In the bioassay in mice, five (41.7%) out of 12 pigeons inoculated were positive to T. gondii. Only one pigeon died at 23 dpi due to toxoplasmosis. A second study with free-living pigeons was performed for detection of anti-T. gondii antibodies. Birds were captured in the municipalities of São Paulo, Ibiúna and Sorocaba, São Paulo State, Southeastern Brazil. All 126 free-living birds were negative to anti-T. gondii antibodies by MAT (titer < 1:5). Bioassays were performed in mice with tissues from all captured birds and T. gondii was not isolated in any pigeon.Colégio Brasileiro de Parasitologia Veterinária2010-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612010000400009Revista Brasileira de Parasitologia Veterinária v.19 n.4 2010reponame:Revista Brasileira de Parasitologia Veterinária (Online)instname:Colégio Brasileiro de Parasitologia Veterinária (CBPV)instacron:CBPV10.1590/S1984-29612010000400009info:eu-repo/semantics/openAccessGodoi,Fernanda Sartori Lima deNishi,Sandra MayumiPena,Hilda Fátima de JesusGennari,Solange Mariaeng2011-02-07T00:00:00Zoai:scielo:S1984-29612010000400009Revistahttp://www.scielo.br/scielo.php?script=sci_serial&lng=pt&pid=1984-2961https://old.scielo.br/oai/scielo-oai.php||zacariascbpv@fcav.unesp.br1984-29610103-846Xopendoar:2011-02-07T00:00Revista Brasileira de Parasitologia Veterinária (Online) - Colégio Brasileiro de Parasitologia Veterinária (CBPV)false
dc.title.none.fl_str_mv Toxoplasma gondii: diagnosis of experimental and natural infection in pigeons (Columba livia) by serological, biological and molecular techniques
title Toxoplasma gondii: diagnosis of experimental and natural infection in pigeons (Columba livia) by serological, biological and molecular techniques
spellingShingle Toxoplasma gondii: diagnosis of experimental and natural infection in pigeons (Columba livia) by serological, biological and molecular techniques
Godoi,Fernanda Sartori Lima de
Toxoplasma gondii
pigeons
experimental infection
bioassay
nested-PCR
title_short Toxoplasma gondii: diagnosis of experimental and natural infection in pigeons (Columba livia) by serological, biological and molecular techniques
title_full Toxoplasma gondii: diagnosis of experimental and natural infection in pigeons (Columba livia) by serological, biological and molecular techniques
title_fullStr Toxoplasma gondii: diagnosis of experimental and natural infection in pigeons (Columba livia) by serological, biological and molecular techniques
title_full_unstemmed Toxoplasma gondii: diagnosis of experimental and natural infection in pigeons (Columba livia) by serological, biological and molecular techniques
title_sort Toxoplasma gondii: diagnosis of experimental and natural infection in pigeons (Columba livia) by serological, biological and molecular techniques
author Godoi,Fernanda Sartori Lima de
author_facet Godoi,Fernanda Sartori Lima de
Nishi,Sandra Mayumi
Pena,Hilda Fátima de Jesus
Gennari,Solange Maria
author_role author
author2 Nishi,Sandra Mayumi
Pena,Hilda Fátima de Jesus
Gennari,Solange Maria
author2_role author
author
author
dc.contributor.author.fl_str_mv Godoi,Fernanda Sartori Lima de
Nishi,Sandra Mayumi
Pena,Hilda Fátima de Jesus
Gennari,Solange Maria
dc.subject.por.fl_str_mv Toxoplasma gondii
pigeons
experimental infection
bioassay
nested-PCR
topic Toxoplasma gondii
pigeons
experimental infection
bioassay
nested-PCR
description This study aimed to diagnose experimental and natural Toxoplasma gondii infection in pigeons (Columba livia) by serological, biological and molecular techniques. Twelve pigeons, free of infection, were inoculated with 50 sporulated oocysts of T. gondii (VEG sample) and four remained uninfected controls. Four birds (three infected and one control) were euthanized at 15, 30, 45 and 60 days post-infection (dpi), and their tissues were used to perform a bioassay in mice and nested-PCR using B1 gene as target. Blood was obtained weekly and it was tested for the presence of anti-T. gondii antibodies by the indirect fluorescent antibody test (IFAT) and modified agglutination test (MAT). Seven (58.3%) out of 12 inoculated pigeons were positive by serological techniques and titers ranged between 1:40 and 1:5120 by MAT and between 1:512 and 1:4096 by IFAT. Complete agreement was seen between the results obtained by serological techniques and nested-PCR in seven positive birds. In the bioassay in mice, five (41.7%) out of 12 pigeons inoculated were positive to T. gondii. Only one pigeon died at 23 dpi due to toxoplasmosis. A second study with free-living pigeons was performed for detection of anti-T. gondii antibodies. Birds were captured in the municipalities of São Paulo, Ibiúna and Sorocaba, São Paulo State, Southeastern Brazil. All 126 free-living birds were negative to anti-T. gondii antibodies by MAT (titer < 1:5). Bioassays were performed in mice with tissues from all captured birds and T. gondii was not isolated in any pigeon.
publishDate 2010
dc.date.none.fl_str_mv 2010-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612010000400009
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dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1984-29612010000400009
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Colégio Brasileiro de Parasitologia Veterinária
publisher.none.fl_str_mv Colégio Brasileiro de Parasitologia Veterinária
dc.source.none.fl_str_mv Revista Brasileira de Parasitologia Veterinária v.19 n.4 2010
reponame:Revista Brasileira de Parasitologia Veterinária (Online)
instname:Colégio Brasileiro de Parasitologia Veterinária (CBPV)
instacron:CBPV
instname_str Colégio Brasileiro de Parasitologia Veterinária (CBPV)
instacron_str CBPV
institution CBPV
reponame_str Revista Brasileira de Parasitologia Veterinária (Online)
collection Revista Brasileira de Parasitologia Veterinária (Online)
repository.name.fl_str_mv Revista Brasileira de Parasitologia Veterinária (Online) - Colégio Brasileiro de Parasitologia Veterinária (CBPV)
repository.mail.fl_str_mv ||zacariascbpv@fcav.unesp.br
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