Use of a Mycoplasma suis-PCR protocol for screening a population of captive peccaries (Tayassu tajacu and Tayassu pecari)
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , , , , , , , , , |
Tipo de documento: | Relatório |
Idioma: | eng |
Título da fonte: | Revista Brasileira de Parasitologia Veterinária (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612011000100016 |
Resumo: | Mycoplasma suis is a hemotropic bacteria of red blood cells and the causative agent of swine eperythrozoonosis. Diagnosis of infection may be reached by direct examination of blood smears; however, the use of polymerase chain reaction (PCR) of the 16S RNA gene of M. suis improves the sensitivity and specificity of detection. The aim of this study was to screen peccaries (Tayassu tajacu and T. pecari) for M. suis infection using a specific conventional PCR. A total of 28 blood samples from captive collared and white-lipped peccaries were collected, DNA extracted and a specific M. suis PCR assay performed. All samples were negatives by both blood smear examination and PCR testing. To verify the presence of amplifiable DNA, PCR for beta-actin gene was performed in all samples. This study was part of an active surveillance program, which is crucial for monitoring animal health status, particularly in wildlife species. |
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Use of a Mycoplasma suis-PCR protocol for screening a population of captive peccaries (Tayassu tajacu and Tayassu pecari)Eperythrozoonosiscollared peccaries (Tayassu tajacu)white-lipped peccaries (Tayassu pecari)wild pigsMycoplasma suis is a hemotropic bacteria of red blood cells and the causative agent of swine eperythrozoonosis. Diagnosis of infection may be reached by direct examination of blood smears; however, the use of polymerase chain reaction (PCR) of the 16S RNA gene of M. suis improves the sensitivity and specificity of detection. The aim of this study was to screen peccaries (Tayassu tajacu and T. pecari) for M. suis infection using a specific conventional PCR. A total of 28 blood samples from captive collared and white-lipped peccaries were collected, DNA extracted and a specific M. suis PCR assay performed. All samples were negatives by both blood smear examination and PCR testing. To verify the presence of amplifiable DNA, PCR for beta-actin gene was performed in all samples. This study was part of an active surveillance program, which is crucial for monitoring animal health status, particularly in wildlife species.Colégio Brasileiro de Parasitologia Veterinária2011-03-01info:eu-repo/semantics/reportinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612011000100016Revista Brasileira de Parasitologia Veterinária v.20 n.1 2011reponame:Revista Brasileira de Parasitologia Veterinária (Online)instname:Colégio Brasileiro de Parasitologia Veterinária (CBPV)instacron:CBPV10.1590/S1984-29612011000100016info:eu-repo/semantics/openAccessVieira,Rafael Felipe da CostaMolento,Marcelo BeltrãoGuimarães,Ana Marcia SáSantos,Andrea Pires dosBonat,MarceloJavorouski,Manoel LucasPopp,LucieneSantos,Leonilda Correia dosMoraes,WanderleiCubas,Zalmir SilvinoVieira,Thállitha Samih Wischral JaymeVidotto,OdilonBarros Filho,Ivan RoqueBiondo,Alexander WelkerMessick,Joanne Belleeng2011-04-20T00:00:00Zoai:scielo:S1984-29612011000100016Revistahttp://www.scielo.br/scielo.php?script=sci_serial&lng=pt&pid=1984-2961https://old.scielo.br/oai/scielo-oai.php||zacariascbpv@fcav.unesp.br1984-29610103-846Xopendoar:2011-04-20T00:00Revista Brasileira de Parasitologia Veterinária (Online) - Colégio Brasileiro de Parasitologia Veterinária (CBPV)false |
dc.title.none.fl_str_mv |
Use of a Mycoplasma suis-PCR protocol for screening a population of captive peccaries (Tayassu tajacu and Tayassu pecari) |
title |
Use of a Mycoplasma suis-PCR protocol for screening a population of captive peccaries (Tayassu tajacu and Tayassu pecari) |
spellingShingle |
Use of a Mycoplasma suis-PCR protocol for screening a population of captive peccaries (Tayassu tajacu and Tayassu pecari) Vieira,Rafael Felipe da Costa Eperythrozoonosis collared peccaries (Tayassu tajacu) white-lipped peccaries (Tayassu pecari) wild pigs |
title_short |
Use of a Mycoplasma suis-PCR protocol for screening a population of captive peccaries (Tayassu tajacu and Tayassu pecari) |
title_full |
Use of a Mycoplasma suis-PCR protocol for screening a population of captive peccaries (Tayassu tajacu and Tayassu pecari) |
title_fullStr |
Use of a Mycoplasma suis-PCR protocol for screening a population of captive peccaries (Tayassu tajacu and Tayassu pecari) |
title_full_unstemmed |
Use of a Mycoplasma suis-PCR protocol for screening a population of captive peccaries (Tayassu tajacu and Tayassu pecari) |
title_sort |
Use of a Mycoplasma suis-PCR protocol for screening a population of captive peccaries (Tayassu tajacu and Tayassu pecari) |
author |
Vieira,Rafael Felipe da Costa |
author_facet |
Vieira,Rafael Felipe da Costa Molento,Marcelo Beltrão Guimarães,Ana Marcia Sá Santos,Andrea Pires dos Bonat,Marcelo Javorouski,Manoel Lucas Popp,Luciene Santos,Leonilda Correia dos Moraes,Wanderlei Cubas,Zalmir Silvino Vieira,Thállitha Samih Wischral Jayme Vidotto,Odilon Barros Filho,Ivan Roque Biondo,Alexander Welker Messick,Joanne Belle |
author_role |
author |
author2 |
Molento,Marcelo Beltrão Guimarães,Ana Marcia Sá Santos,Andrea Pires dos Bonat,Marcelo Javorouski,Manoel Lucas Popp,Luciene Santos,Leonilda Correia dos Moraes,Wanderlei Cubas,Zalmir Silvino Vieira,Thállitha Samih Wischral Jayme Vidotto,Odilon Barros Filho,Ivan Roque Biondo,Alexander Welker Messick,Joanne Belle |
author2_role |
author author author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Vieira,Rafael Felipe da Costa Molento,Marcelo Beltrão Guimarães,Ana Marcia Sá Santos,Andrea Pires dos Bonat,Marcelo Javorouski,Manoel Lucas Popp,Luciene Santos,Leonilda Correia dos Moraes,Wanderlei Cubas,Zalmir Silvino Vieira,Thállitha Samih Wischral Jayme Vidotto,Odilon Barros Filho,Ivan Roque Biondo,Alexander Welker Messick,Joanne Belle |
dc.subject.por.fl_str_mv |
Eperythrozoonosis collared peccaries (Tayassu tajacu) white-lipped peccaries (Tayassu pecari) wild pigs |
topic |
Eperythrozoonosis collared peccaries (Tayassu tajacu) white-lipped peccaries (Tayassu pecari) wild pigs |
description |
Mycoplasma suis is a hemotropic bacteria of red blood cells and the causative agent of swine eperythrozoonosis. Diagnosis of infection may be reached by direct examination of blood smears; however, the use of polymerase chain reaction (PCR) of the 16S RNA gene of M. suis improves the sensitivity and specificity of detection. The aim of this study was to screen peccaries (Tayassu tajacu and T. pecari) for M. suis infection using a specific conventional PCR. A total of 28 blood samples from captive collared and white-lipped peccaries were collected, DNA extracted and a specific M. suis PCR assay performed. All samples were negatives by both blood smear examination and PCR testing. To verify the presence of amplifiable DNA, PCR for beta-actin gene was performed in all samples. This study was part of an active surveillance program, which is crucial for monitoring animal health status, particularly in wildlife species. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-03-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/report |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
report |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612011000100016 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612011000100016 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1984-29612011000100016 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Colégio Brasileiro de Parasitologia Veterinária |
publisher.none.fl_str_mv |
Colégio Brasileiro de Parasitologia Veterinária |
dc.source.none.fl_str_mv |
Revista Brasileira de Parasitologia Veterinária v.20 n.1 2011 reponame:Revista Brasileira de Parasitologia Veterinária (Online) instname:Colégio Brasileiro de Parasitologia Veterinária (CBPV) instacron:CBPV |
instname_str |
Colégio Brasileiro de Parasitologia Veterinária (CBPV) |
instacron_str |
CBPV |
institution |
CBPV |
reponame_str |
Revista Brasileira de Parasitologia Veterinária (Online) |
collection |
Revista Brasileira de Parasitologia Veterinária (Online) |
repository.name.fl_str_mv |
Revista Brasileira de Parasitologia Veterinária (Online) - Colégio Brasileiro de Parasitologia Veterinária (CBPV) |
repository.mail.fl_str_mv |
||zacariascbpv@fcav.unesp.br |
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1754208913975148544 |