First description of Cryptosporidium parvum in carrier pigeons (Columba livia)
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.vetpar.2017.06.023 http://hdl.handle.net/11449/178993 |
Resumo: | The carrier pigeon and the domestic pigeon are different breeds of the species Columba livia. Carrier pigeons are used for recreational activities such as bird contests and exhibitions. Due to the close contact with humans, these birds may potentially represent a public health risk, since they can host and disseminate zoonotic parasites, such as those belonging to the genus Cryptosporidium (phylum Apicomplexa). The purpose of this work was the detection by microscopic and molecular techniques of Cryptosporidium spp. oocysts in fecal samples of carrier pigeons, and subsequently to sequence the 18S ribosomal RNA marker of positive samples to identify the species. A total of 100 fecal samples were collected individually in two pigeon breeding facilities from Formiga and Araçatuba, cities located in Minas Gerais state and São Paulo state, Brazil, respectively. The age of the birds ranged from one to 12 years; 56 were females and 44 males. Fecal smears were stained with negative malachite green, whereas the molecular characterization was based on the sequence of a ∼800 bp fragment of the 18S rRNA gene. Microscopic examination of fecal smears revealed 4% (4/100) oocyst positivity. On the other hand, 7% (7/100) of positivity were found using nested PCR. Three samples were 99% to 100% similar to Cryptosporidium parvum 18S rDNA type A (Genbank AH006572) and the other three samples had 99% to 100% similarity to C. parvum 18S rDNA type B (Genbank AF308600). To our knowledge, this is the first report of C. parvum oocysts in carrier pigeons. |
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First description of Cryptosporidium parvum in carrier pigeons (Columba livia)BirdsCarrier pigeonsColumba liviaCryptosporidiosisCryptosporidium parvumNested PCRThe carrier pigeon and the domestic pigeon are different breeds of the species Columba livia. Carrier pigeons are used for recreational activities such as bird contests and exhibitions. Due to the close contact with humans, these birds may potentially represent a public health risk, since they can host and disseminate zoonotic parasites, such as those belonging to the genus Cryptosporidium (phylum Apicomplexa). The purpose of this work was the detection by microscopic and molecular techniques of Cryptosporidium spp. oocysts in fecal samples of carrier pigeons, and subsequently to sequence the 18S ribosomal RNA marker of positive samples to identify the species. A total of 100 fecal samples were collected individually in two pigeon breeding facilities from Formiga and Araçatuba, cities located in Minas Gerais state and São Paulo state, Brazil, respectively. The age of the birds ranged from one to 12 years; 56 were females and 44 males. Fecal smears were stained with negative malachite green, whereas the molecular characterization was based on the sequence of a ∼800 bp fragment of the 18S rRNA gene. Microscopic examination of fecal smears revealed 4% (4/100) oocyst positivity. On the other hand, 7% (7/100) of positivity were found using nested PCR. Three samples were 99% to 100% similar to Cryptosporidium parvum 18S rDNA type A (Genbank AH006572) and the other three samples had 99% to 100% similarity to C. parvum 18S rDNA type B (Genbank AF308600). To our knowledge, this is the first report of C. parvum oocysts in carrier pigeons.Universidade Estadual Paulista (Unesp) Faculdade de Medicina VeterináriaLaboratory of Visual Informatics in Biomedical and Health Institute of Computing University of Campinas – UNICAMPDepartment of Infectious Disease & Global Health Cummings School of Veterinary Medicine at Tufts UniversityUniversidade Estadual Paulista (Unesp) Faculdade de Medicina Veterinária, Araçatuba. Rua Clóvis Pestana, 793, Jardim Dona Amélia, AraçatubaUniversidade Estadual Paulista (Unesp) Faculdade de Medicina VeterináriaUniversidade Estadual Paulista (Unesp) Faculdade de Medicina Veterinária, Araçatuba. Rua Clóvis Pestana, 793, Jardim Dona Amélia, AraçatubaUniversidade Estadual Paulista (Unesp)Universidade Estadual de Campinas (UNICAMP)Cummings School of Veterinary Medicine at Tufts UniversityOliveira, Bruno César Miranda [UNESP]Ferrari, Elis Domingos [UNESP]da Cruz Panegossi, Mariele Fernanda [UNESP]Nakamura, Alex Akira [UNESP]Corbucci, Flávio Sader [UNESP]Nagata, Walter Bertequini [UNESP]dos Santos, Bianca MartinsGomes, Jancarlo FerreiraMeireles, Marcelo Vasconcelos [UNESP]Widmer, GiovanniBresciani, Katia Denise Saraiva [UNESP]2018-12-11T17:33:04Z2018-12-11T17:33:04Z2017-08-30info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article148-150application/pdfhttp://dx.doi.org/10.1016/j.vetpar.2017.06.023Veterinary Parasitology, v. 243, p. 148-150.1873-25500304-4017http://hdl.handle.net/11449/17899310.1016/j.vetpar.2017.06.0232-s2.0-850218052962-s2.0-85021805296.pdf59505943668296470903513897615274Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengVeterinary Parasitology1,275info:eu-repo/semantics/openAccess2024-09-04T19:15:51Zoai:repositorio.unesp.br:11449/178993Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-04T19:15:51Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
First description of Cryptosporidium parvum in carrier pigeons (Columba livia) |
title |
First description of Cryptosporidium parvum in carrier pigeons (Columba livia) |
spellingShingle |
First description of Cryptosporidium parvum in carrier pigeons (Columba livia) Oliveira, Bruno César Miranda [UNESP] Birds Carrier pigeons Columba livia Cryptosporidiosis Cryptosporidium parvum Nested PCR |
title_short |
First description of Cryptosporidium parvum in carrier pigeons (Columba livia) |
title_full |
First description of Cryptosporidium parvum in carrier pigeons (Columba livia) |
title_fullStr |
First description of Cryptosporidium parvum in carrier pigeons (Columba livia) |
title_full_unstemmed |
First description of Cryptosporidium parvum in carrier pigeons (Columba livia) |
title_sort |
First description of Cryptosporidium parvum in carrier pigeons (Columba livia) |
author |
Oliveira, Bruno César Miranda [UNESP] |
author_facet |
Oliveira, Bruno César Miranda [UNESP] Ferrari, Elis Domingos [UNESP] da Cruz Panegossi, Mariele Fernanda [UNESP] Nakamura, Alex Akira [UNESP] Corbucci, Flávio Sader [UNESP] Nagata, Walter Bertequini [UNESP] dos Santos, Bianca Martins Gomes, Jancarlo Ferreira Meireles, Marcelo Vasconcelos [UNESP] Widmer, Giovanni Bresciani, Katia Denise Saraiva [UNESP] |
author_role |
author |
author2 |
Ferrari, Elis Domingos [UNESP] da Cruz Panegossi, Mariele Fernanda [UNESP] Nakamura, Alex Akira [UNESP] Corbucci, Flávio Sader [UNESP] Nagata, Walter Bertequini [UNESP] dos Santos, Bianca Martins Gomes, Jancarlo Ferreira Meireles, Marcelo Vasconcelos [UNESP] Widmer, Giovanni Bresciani, Katia Denise Saraiva [UNESP] |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Estadual de Campinas (UNICAMP) Cummings School of Veterinary Medicine at Tufts University |
dc.contributor.author.fl_str_mv |
Oliveira, Bruno César Miranda [UNESP] Ferrari, Elis Domingos [UNESP] da Cruz Panegossi, Mariele Fernanda [UNESP] Nakamura, Alex Akira [UNESP] Corbucci, Flávio Sader [UNESP] Nagata, Walter Bertequini [UNESP] dos Santos, Bianca Martins Gomes, Jancarlo Ferreira Meireles, Marcelo Vasconcelos [UNESP] Widmer, Giovanni Bresciani, Katia Denise Saraiva [UNESP] |
dc.subject.por.fl_str_mv |
Birds Carrier pigeons Columba livia Cryptosporidiosis Cryptosporidium parvum Nested PCR |
topic |
Birds Carrier pigeons Columba livia Cryptosporidiosis Cryptosporidium parvum Nested PCR |
description |
The carrier pigeon and the domestic pigeon are different breeds of the species Columba livia. Carrier pigeons are used for recreational activities such as bird contests and exhibitions. Due to the close contact with humans, these birds may potentially represent a public health risk, since they can host and disseminate zoonotic parasites, such as those belonging to the genus Cryptosporidium (phylum Apicomplexa). The purpose of this work was the detection by microscopic and molecular techniques of Cryptosporidium spp. oocysts in fecal samples of carrier pigeons, and subsequently to sequence the 18S ribosomal RNA marker of positive samples to identify the species. A total of 100 fecal samples were collected individually in two pigeon breeding facilities from Formiga and Araçatuba, cities located in Minas Gerais state and São Paulo state, Brazil, respectively. The age of the birds ranged from one to 12 years; 56 were females and 44 males. Fecal smears were stained with negative malachite green, whereas the molecular characterization was based on the sequence of a ∼800 bp fragment of the 18S rRNA gene. Microscopic examination of fecal smears revealed 4% (4/100) oocyst positivity. On the other hand, 7% (7/100) of positivity were found using nested PCR. Three samples were 99% to 100% similar to Cryptosporidium parvum 18S rDNA type A (Genbank AH006572) and the other three samples had 99% to 100% similarity to C. parvum 18S rDNA type B (Genbank AF308600). To our knowledge, this is the first report of C. parvum oocysts in carrier pigeons. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-08-30 2018-12-11T17:33:04Z 2018-12-11T17:33:04Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.vetpar.2017.06.023 Veterinary Parasitology, v. 243, p. 148-150. 1873-2550 0304-4017 http://hdl.handle.net/11449/178993 10.1016/j.vetpar.2017.06.023 2-s2.0-85021805296 2-s2.0-85021805296.pdf 5950594366829647 0903513897615274 |
url |
http://dx.doi.org/10.1016/j.vetpar.2017.06.023 http://hdl.handle.net/11449/178993 |
identifier_str_mv |
Veterinary Parasitology, v. 243, p. 148-150. 1873-2550 0304-4017 10.1016/j.vetpar.2017.06.023 2-s2.0-85021805296 2-s2.0-85021805296.pdf 5950594366829647 0903513897615274 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Veterinary Parasitology 1,275 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
148-150 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1810021400570757120 |