First description of Cryptosporidium parvum in carrier pigeons (Columba livia)

Detalhes bibliográficos
Autor(a) principal: Oliveira, Bruno César Miranda [UNESP]
Data de Publicação: 2017
Outros Autores: Ferrari, Elis Domingos [UNESP], da Cruz Panegossi, Mariele Fernanda [UNESP], Nakamura, Alex Akira [UNESP], Corbucci, Flávio Sader [UNESP], Nagata, Walter Bertequini [UNESP], dos Santos, Bianca Martins, Gomes, Jancarlo Ferreira, Meireles, Marcelo Vasconcelos [UNESP], Widmer, Giovanni, Bresciani, Katia Denise Saraiva [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.vetpar.2017.06.023
http://hdl.handle.net/11449/178993
Resumo: The carrier pigeon and the domestic pigeon are different breeds of the species Columba livia. Carrier pigeons are used for recreational activities such as bird contests and exhibitions. Due to the close contact with humans, these birds may potentially represent a public health risk, since they can host and disseminate zoonotic parasites, such as those belonging to the genus Cryptosporidium (phylum Apicomplexa). The purpose of this work was the detection by microscopic and molecular techniques of Cryptosporidium spp. oocysts in fecal samples of carrier pigeons, and subsequently to sequence the 18S ribosomal RNA marker of positive samples to identify the species. A total of 100 fecal samples were collected individually in two pigeon breeding facilities from Formiga and Araçatuba, cities located in Minas Gerais state and São Paulo state, Brazil, respectively. The age of the birds ranged from one to 12 years; 56 were females and 44 males. Fecal smears were stained with negative malachite green, whereas the molecular characterization was based on the sequence of a ∼800 bp fragment of the 18S rRNA gene. Microscopic examination of fecal smears revealed 4% (4/100) oocyst positivity. On the other hand, 7% (7/100) of positivity were found using nested PCR. Three samples were 99% to 100% similar to Cryptosporidium parvum 18S rDNA type A (Genbank AH006572) and the other three samples had 99% to 100% similarity to C. parvum 18S rDNA type B (Genbank AF308600). To our knowledge, this is the first report of C. parvum oocysts in carrier pigeons.
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spelling First description of Cryptosporidium parvum in carrier pigeons (Columba livia)BirdsCarrier pigeonsColumba liviaCryptosporidiosisCryptosporidium parvumNested PCRThe carrier pigeon and the domestic pigeon are different breeds of the species Columba livia. Carrier pigeons are used for recreational activities such as bird contests and exhibitions. Due to the close contact with humans, these birds may potentially represent a public health risk, since they can host and disseminate zoonotic parasites, such as those belonging to the genus Cryptosporidium (phylum Apicomplexa). The purpose of this work was the detection by microscopic and molecular techniques of Cryptosporidium spp. oocysts in fecal samples of carrier pigeons, and subsequently to sequence the 18S ribosomal RNA marker of positive samples to identify the species. A total of 100 fecal samples were collected individually in two pigeon breeding facilities from Formiga and Araçatuba, cities located in Minas Gerais state and São Paulo state, Brazil, respectively. The age of the birds ranged from one to 12 years; 56 were females and 44 males. Fecal smears were stained with negative malachite green, whereas the molecular characterization was based on the sequence of a ∼800 bp fragment of the 18S rRNA gene. Microscopic examination of fecal smears revealed 4% (4/100) oocyst positivity. On the other hand, 7% (7/100) of positivity were found using nested PCR. Three samples were 99% to 100% similar to Cryptosporidium parvum 18S rDNA type A (Genbank AH006572) and the other three samples had 99% to 100% similarity to C. parvum 18S rDNA type B (Genbank AF308600). To our knowledge, this is the first report of C. parvum oocysts in carrier pigeons.Universidade Estadual Paulista (Unesp) Faculdade de Medicina VeterináriaLaboratory of Visual Informatics in Biomedical and Health Institute of Computing University of Campinas – UNICAMPDepartment of Infectious Disease & Global Health Cummings School of Veterinary Medicine at Tufts UniversityUniversidade Estadual Paulista (Unesp) Faculdade de Medicina Veterinária, Araçatuba. Rua Clóvis Pestana, 793, Jardim Dona Amélia, AraçatubaUniversidade Estadual Paulista (Unesp) Faculdade de Medicina VeterináriaUniversidade Estadual Paulista (Unesp) Faculdade de Medicina Veterinária, Araçatuba. Rua Clóvis Pestana, 793, Jardim Dona Amélia, AraçatubaUniversidade Estadual Paulista (Unesp)Universidade Estadual de Campinas (UNICAMP)Cummings School of Veterinary Medicine at Tufts UniversityOliveira, Bruno César Miranda [UNESP]Ferrari, Elis Domingos [UNESP]da Cruz Panegossi, Mariele Fernanda [UNESP]Nakamura, Alex Akira [UNESP]Corbucci, Flávio Sader [UNESP]Nagata, Walter Bertequini [UNESP]dos Santos, Bianca MartinsGomes, Jancarlo FerreiraMeireles, Marcelo Vasconcelos [UNESP]Widmer, GiovanniBresciani, Katia Denise Saraiva [UNESP]2018-12-11T17:33:04Z2018-12-11T17:33:04Z2017-08-30info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article148-150application/pdfhttp://dx.doi.org/10.1016/j.vetpar.2017.06.023Veterinary Parasitology, v. 243, p. 148-150.1873-25500304-4017http://hdl.handle.net/11449/17899310.1016/j.vetpar.2017.06.0232-s2.0-850218052962-s2.0-85021805296.pdf59505943668296470903513897615274Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengVeterinary Parasitology1,275info:eu-repo/semantics/openAccess2024-09-04T19:15:51Zoai:repositorio.unesp.br:11449/178993Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-04T19:15:51Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv First description of Cryptosporidium parvum in carrier pigeons (Columba livia)
title First description of Cryptosporidium parvum in carrier pigeons (Columba livia)
spellingShingle First description of Cryptosporidium parvum in carrier pigeons (Columba livia)
Oliveira, Bruno César Miranda [UNESP]
Birds
Carrier pigeons
Columba livia
Cryptosporidiosis
Cryptosporidium parvum
Nested PCR
title_short First description of Cryptosporidium parvum in carrier pigeons (Columba livia)
title_full First description of Cryptosporidium parvum in carrier pigeons (Columba livia)
title_fullStr First description of Cryptosporidium parvum in carrier pigeons (Columba livia)
title_full_unstemmed First description of Cryptosporidium parvum in carrier pigeons (Columba livia)
title_sort First description of Cryptosporidium parvum in carrier pigeons (Columba livia)
author Oliveira, Bruno César Miranda [UNESP]
author_facet Oliveira, Bruno César Miranda [UNESP]
Ferrari, Elis Domingos [UNESP]
da Cruz Panegossi, Mariele Fernanda [UNESP]
Nakamura, Alex Akira [UNESP]
Corbucci, Flávio Sader [UNESP]
Nagata, Walter Bertequini [UNESP]
dos Santos, Bianca Martins
Gomes, Jancarlo Ferreira
Meireles, Marcelo Vasconcelos [UNESP]
Widmer, Giovanni
Bresciani, Katia Denise Saraiva [UNESP]
author_role author
author2 Ferrari, Elis Domingos [UNESP]
da Cruz Panegossi, Mariele Fernanda [UNESP]
Nakamura, Alex Akira [UNESP]
Corbucci, Flávio Sader [UNESP]
Nagata, Walter Bertequini [UNESP]
dos Santos, Bianca Martins
Gomes, Jancarlo Ferreira
Meireles, Marcelo Vasconcelos [UNESP]
Widmer, Giovanni
Bresciani, Katia Denise Saraiva [UNESP]
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade Estadual de Campinas (UNICAMP)
Cummings School of Veterinary Medicine at Tufts University
dc.contributor.author.fl_str_mv Oliveira, Bruno César Miranda [UNESP]
Ferrari, Elis Domingos [UNESP]
da Cruz Panegossi, Mariele Fernanda [UNESP]
Nakamura, Alex Akira [UNESP]
Corbucci, Flávio Sader [UNESP]
Nagata, Walter Bertequini [UNESP]
dos Santos, Bianca Martins
Gomes, Jancarlo Ferreira
Meireles, Marcelo Vasconcelos [UNESP]
Widmer, Giovanni
Bresciani, Katia Denise Saraiva [UNESP]
dc.subject.por.fl_str_mv Birds
Carrier pigeons
Columba livia
Cryptosporidiosis
Cryptosporidium parvum
Nested PCR
topic Birds
Carrier pigeons
Columba livia
Cryptosporidiosis
Cryptosporidium parvum
Nested PCR
description The carrier pigeon and the domestic pigeon are different breeds of the species Columba livia. Carrier pigeons are used for recreational activities such as bird contests and exhibitions. Due to the close contact with humans, these birds may potentially represent a public health risk, since they can host and disseminate zoonotic parasites, such as those belonging to the genus Cryptosporidium (phylum Apicomplexa). The purpose of this work was the detection by microscopic and molecular techniques of Cryptosporidium spp. oocysts in fecal samples of carrier pigeons, and subsequently to sequence the 18S ribosomal RNA marker of positive samples to identify the species. A total of 100 fecal samples were collected individually in two pigeon breeding facilities from Formiga and Araçatuba, cities located in Minas Gerais state and São Paulo state, Brazil, respectively. The age of the birds ranged from one to 12 years; 56 were females and 44 males. Fecal smears were stained with negative malachite green, whereas the molecular characterization was based on the sequence of a ∼800 bp fragment of the 18S rRNA gene. Microscopic examination of fecal smears revealed 4% (4/100) oocyst positivity. On the other hand, 7% (7/100) of positivity were found using nested PCR. Three samples were 99% to 100% similar to Cryptosporidium parvum 18S rDNA type A (Genbank AH006572) and the other three samples had 99% to 100% similarity to C. parvum 18S rDNA type B (Genbank AF308600). To our knowledge, this is the first report of C. parvum oocysts in carrier pigeons.
publishDate 2017
dc.date.none.fl_str_mv 2017-08-30
2018-12-11T17:33:04Z
2018-12-11T17:33:04Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.vetpar.2017.06.023
Veterinary Parasitology, v. 243, p. 148-150.
1873-2550
0304-4017
http://hdl.handle.net/11449/178993
10.1016/j.vetpar.2017.06.023
2-s2.0-85021805296
2-s2.0-85021805296.pdf
5950594366829647
0903513897615274
url http://dx.doi.org/10.1016/j.vetpar.2017.06.023
http://hdl.handle.net/11449/178993
identifier_str_mv Veterinary Parasitology, v. 243, p. 148-150.
1873-2550
0304-4017
10.1016/j.vetpar.2017.06.023
2-s2.0-85021805296
2-s2.0-85021805296.pdf
5950594366829647
0903513897615274
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Veterinary Parasitology
1,275
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 148-150
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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