Detection of Hemotropic Mycoplasma sp. in white-eared opossums (Didelphis albiventris) from Southern Brazil

Detalhes bibliográficos
Autor(a) principal: Massini,Paula Fernanda
Data de Publicação: 2019
Outros Autores: Drozino,Ricardo Nascimento, Otomura,Flávio Haragushiku, Mongruel,Anna Claudia Baumel, Valente,Jessica Damiana Marinho, Toledo,Max Jean de Ornelas, Martins,Thiago Fernandes, Vidotto,Odilon, Vieira,Thállitha Samih Wischral Jaime, Vieira,Rafael Felipe da Costa
Tipo de documento: Relatório
Idioma: eng
Título da fonte: Revista Brasileira de Parasitologia Veterinária (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612019000400797
Resumo: Abstract Opossums are marsupials from the New World of the genus Didelphis and known as synanthropic animals due to their proximity with human beings. To date, ‘Candidatus Mycoplasma haemodidelphis’ has been solely found infecting the North American opossum (Didelphis virginiana). Accordingly, the aim of this study was to screen eight white-eared opossums (Didelphis albiventris) from a public park in Maringa city, Paraná State, southern Brazil, for hemoplasma infection. Blood samples were taken from caudal venipuncture, and DNA was extracted and further screened by a pan-hemoplasma PCR assay. Seven out of eight (87.50%; CI 95%: 47.35-99.68%) white-eared opossums were positive for Mycoplasma spp. Sequencing of the 16S rRNA fragment showed 98,97% identity with ‘Ca. M. haemodidelphis’ detected in the USA. Three out of eight (37.50%; CI 95%: 8.52-75.51%) white-eared opossums were infested by Amblyomma dubitatum ticks. This is the first report on detection of a potentially novel hemotropic Mycoplasma sp. infecting opossums from South America.
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spelling Detection of Hemotropic Mycoplasma sp. in white-eared opossums (Didelphis albiventris) from Southern BrazilMarsupialshemotropic mycoplasmashemoplasmasMycoplasma spAbstract Opossums are marsupials from the New World of the genus Didelphis and known as synanthropic animals due to their proximity with human beings. To date, ‘Candidatus Mycoplasma haemodidelphis’ has been solely found infecting the North American opossum (Didelphis virginiana). Accordingly, the aim of this study was to screen eight white-eared opossums (Didelphis albiventris) from a public park in Maringa city, Paraná State, southern Brazil, for hemoplasma infection. Blood samples were taken from caudal venipuncture, and DNA was extracted and further screened by a pan-hemoplasma PCR assay. Seven out of eight (87.50%; CI 95%: 47.35-99.68%) white-eared opossums were positive for Mycoplasma spp. Sequencing of the 16S rRNA fragment showed 98,97% identity with ‘Ca. M. haemodidelphis’ detected in the USA. Three out of eight (37.50%; CI 95%: 8.52-75.51%) white-eared opossums were infested by Amblyomma dubitatum ticks. This is the first report on detection of a potentially novel hemotropic Mycoplasma sp. infecting opossums from South America.Colégio Brasileiro de Parasitologia Veterinária2019-12-01info:eu-repo/semantics/reportinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612019000400797Revista Brasileira de Parasitologia Veterinária v.28 n.4 2019reponame:Revista Brasileira de Parasitologia Veterinária (Online)instname:Colégio Brasileiro de Parasitologia Veterinária (CBPV)instacron:CBPV10.1590/s1984-29612019058info:eu-repo/semantics/openAccessMassini,Paula FernandaDrozino,Ricardo NascimentoOtomura,Flávio HaragushikuMongruel,Anna Claudia BaumelValente,Jessica Damiana MarinhoToledo,Max Jean de OrnelasMartins,Thiago FernandesVidotto,OdilonVieira,Thállitha Samih Wischral JaimeVieira,Rafael Felipe da Costaeng2020-04-14T00:00:00Zoai:scielo:S1984-29612019000400797Revistahttp://www.scielo.br/scielo.php?script=sci_serial&lng=pt&pid=1984-2961https://old.scielo.br/oai/scielo-oai.php||zacariascbpv@fcav.unesp.br1984-29610103-846Xopendoar:2020-04-14T00:00Revista Brasileira de Parasitologia Veterinária (Online) - Colégio Brasileiro de Parasitologia Veterinária (CBPV)false
dc.title.none.fl_str_mv Detection of Hemotropic Mycoplasma sp. in white-eared opossums (Didelphis albiventris) from Southern Brazil
title Detection of Hemotropic Mycoplasma sp. in white-eared opossums (Didelphis albiventris) from Southern Brazil
spellingShingle Detection of Hemotropic Mycoplasma sp. in white-eared opossums (Didelphis albiventris) from Southern Brazil
Massini,Paula Fernanda
Marsupials
hemotropic mycoplasmas
hemoplasmas
Mycoplasma sp
title_short Detection of Hemotropic Mycoplasma sp. in white-eared opossums (Didelphis albiventris) from Southern Brazil
title_full Detection of Hemotropic Mycoplasma sp. in white-eared opossums (Didelphis albiventris) from Southern Brazil
title_fullStr Detection of Hemotropic Mycoplasma sp. in white-eared opossums (Didelphis albiventris) from Southern Brazil
title_full_unstemmed Detection of Hemotropic Mycoplasma sp. in white-eared opossums (Didelphis albiventris) from Southern Brazil
title_sort Detection of Hemotropic Mycoplasma sp. in white-eared opossums (Didelphis albiventris) from Southern Brazil
author Massini,Paula Fernanda
author_facet Massini,Paula Fernanda
Drozino,Ricardo Nascimento
Otomura,Flávio Haragushiku
Mongruel,Anna Claudia Baumel
Valente,Jessica Damiana Marinho
Toledo,Max Jean de Ornelas
Martins,Thiago Fernandes
Vidotto,Odilon
Vieira,Thállitha Samih Wischral Jaime
Vieira,Rafael Felipe da Costa
author_role author
author2 Drozino,Ricardo Nascimento
Otomura,Flávio Haragushiku
Mongruel,Anna Claudia Baumel
Valente,Jessica Damiana Marinho
Toledo,Max Jean de Ornelas
Martins,Thiago Fernandes
Vidotto,Odilon
Vieira,Thállitha Samih Wischral Jaime
Vieira,Rafael Felipe da Costa
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Massini,Paula Fernanda
Drozino,Ricardo Nascimento
Otomura,Flávio Haragushiku
Mongruel,Anna Claudia Baumel
Valente,Jessica Damiana Marinho
Toledo,Max Jean de Ornelas
Martins,Thiago Fernandes
Vidotto,Odilon
Vieira,Thállitha Samih Wischral Jaime
Vieira,Rafael Felipe da Costa
dc.subject.por.fl_str_mv Marsupials
hemotropic mycoplasmas
hemoplasmas
Mycoplasma sp
topic Marsupials
hemotropic mycoplasmas
hemoplasmas
Mycoplasma sp
description Abstract Opossums are marsupials from the New World of the genus Didelphis and known as synanthropic animals due to their proximity with human beings. To date, ‘Candidatus Mycoplasma haemodidelphis’ has been solely found infecting the North American opossum (Didelphis virginiana). Accordingly, the aim of this study was to screen eight white-eared opossums (Didelphis albiventris) from a public park in Maringa city, Paraná State, southern Brazil, for hemoplasma infection. Blood samples were taken from caudal venipuncture, and DNA was extracted and further screened by a pan-hemoplasma PCR assay. Seven out of eight (87.50%; CI 95%: 47.35-99.68%) white-eared opossums were positive for Mycoplasma spp. Sequencing of the 16S rRNA fragment showed 98,97% identity with ‘Ca. M. haemodidelphis’ detected in the USA. Three out of eight (37.50%; CI 95%: 8.52-75.51%) white-eared opossums were infested by Amblyomma dubitatum ticks. This is the first report on detection of a potentially novel hemotropic Mycoplasma sp. infecting opossums from South America.
publishDate 2019
dc.date.none.fl_str_mv 2019-12-01
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dc.language.iso.fl_str_mv eng
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dc.relation.none.fl_str_mv 10.1590/s1984-29612019058
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dc.publisher.none.fl_str_mv Colégio Brasileiro de Parasitologia Veterinária
publisher.none.fl_str_mv Colégio Brasileiro de Parasitologia Veterinária
dc.source.none.fl_str_mv Revista Brasileira de Parasitologia Veterinária v.28 n.4 2019
reponame:Revista Brasileira de Parasitologia Veterinária (Online)
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