ANTIMICROBIAL SUSCEPTIBILIT Y OF BACTERIA IN CHRONIC ULCERS TO CRUDE EXTRACTS OF Prosopis juliflora
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Saúde e Pesquisa (Online) |
Texto Completo: | https://periodicos.unicesumar.edu.br/index.php/saudpesq/article/view/4488 |
Resumo: | Chronic ulcers are prone to infection due to the sterile lossof the innate barrier of the skin and cutaneous annexes, coupled to the development of microbial communities. Current study investigates the susceptibility of bacteria associated with chronic ulcers to crude extracts of Prosopis juliflora. In vitro tests with the crude extracts of leaves and stem bark were performed against strains of Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 27853), Staphylococcus aureus (ATCC 25923) and Proteus mirabilis (ATCC 7002), by a diffusion assay in agar and determination of Minimum Inhibiory Concentration (MIC). Results showed that E. coli, P. aeruginosa, S. aureus and P.mirabilis strains were affected by the crude extracts of the leaves, with a diameter of the inhibition zone measuring respectively 21.0; 33.0; 20.0; 21.0 mm, whereas E. coli and S. aureus were more affected to the crude extracts of the stem bark, with an inhibition zone diameter of 21.5 and 20.0 mm, respectively. Best MIC rates were reported for the crude extracts of P. juliflora leaves. Chemical studies for the extraction and isolation of compounds are recommended for the execution of in vitro assays to investigate the compounds´ antimicrobial activity. |
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ANTIMICROBIAL SUSCEPTIBILIT Y OF BACTERIA IN CHRONIC ULCERS TO CRUDE EXTRACTS OF Prosopis julifloraSusceptibilidade Antimicrobiana de Bactérias Ocorrentes em Úlceras Crônicas aos Extratos Brutoss de Prosopis JulifloraProsopis julifloraÚlceras crônicasAtividade antimicrobianaChronic ulcers are prone to infection due to the sterile lossof the innate barrier of the skin and cutaneous annexes, coupled to the development of microbial communities. Current study investigates the susceptibility of bacteria associated with chronic ulcers to crude extracts of Prosopis juliflora. In vitro tests with the crude extracts of leaves and stem bark were performed against strains of Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 27853), Staphylococcus aureus (ATCC 25923) and Proteus mirabilis (ATCC 7002), by a diffusion assay in agar and determination of Minimum Inhibiory Concentration (MIC). Results showed that E. coli, P. aeruginosa, S. aureus and P.mirabilis strains were affected by the crude extracts of the leaves, with a diameter of the inhibition zone measuring respectively 21.0; 33.0; 20.0; 21.0 mm, whereas E. coli and S. aureus were more affected to the crude extracts of the stem bark, with an inhibition zone diameter of 21.5 and 20.0 mm, respectively. Best MIC rates were reported for the crude extracts of P. juliflora leaves. Chemical studies for the extraction and isolation of compounds are recommended for the execution of in vitro assays to investigate the compounds´ antimicrobial activity.Úlceras crônicas são suscetíveis à infecção devido à perda estéril da função de barreira inata da pele e dos anexos cutâneos, facilitando o desenvolvimento de comunidades microbianas. O objetivo do estudo foi investigar a suscetibilidade de bactérias associadas a úlceras crônicas aos extratos brutos de Prosopis juliflora. Os testes in vitro foram realizados com extratos brutos das folhas e casca do caule contra as estirpes de Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 27853), Staphylococcus aureus (ATCC 25923) e Proteus mirabilis (ATCC 7002), por meio de ensaio de difusão em agar com poço e determinação da Concentrações Inibitórias Mínimas (MIC). Os resultados mostraram que as estirpes de E. coli, P. aeruginosa, S. aureus e P. mirabilis foram mais susceptíveis aos extratos brutos da folha, com diâmetro de zona de inibição de 21,0; 33,0; 20,0; 21,0 mm, respectivamente, enquanto, E. coli e S. aureus foram mais susceptíveis aos extratos brutos da casca do caule, com diâmetro da zona de inibição de 21,5 e 20,0 mm, respectivamente. Os melhores valores de CIM foram registrados para EBF de P. juliflora. Estudo químico para a extração e o isolamento de compostos ativos é recomendado para realização de ensaios in vitro para investigar a atividade antimicrobiana destes compostos.Universidade Cesumar - UniCesumar2015-12-31info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAvaliado por Paresapplication/pdfhttps://periodicos.unicesumar.edu.br/index.php/saudpesq/article/view/448810.17765/1983-1870.2015v8n3p493-499Saúde e Pesquisa; Vol 8 No 3 (2015): set./dez.; 493-499Saúde e Pesquisa; v. 8 n. 3 (2015): set./dez.; 493-4992176-9206reponame:Saúde e Pesquisa (Online)instname:Cesumar Diretoria de Pesquisainstacron:CESUMARporhttps://periodicos.unicesumar.edu.br/index.php/saudpesq/article/view/4488/2697Silva, Renata Soares daOliveira, Karla Maria Santos deCavalcante, Giani Mariainfo:eu-repo/semantics/openAccess2021-12-09T17:15:35Zoai:ojs.pkp.sfu.ca:article/4488Revistahttps://periodicos.unicesumar.edu.br/index.php/saudpesqPUBhttps://periodicos.unicesumar.edu.br/index.php/saudpesq/oainaep@cesumar.br2176-92061983-1870opendoar:2021-12-09T17:15:35Saúde e Pesquisa (Online) - Cesumar Diretoria de Pesquisafalse |
dc.title.none.fl_str_mv |
ANTIMICROBIAL SUSCEPTIBILIT Y OF BACTERIA IN CHRONIC ULCERS TO CRUDE EXTRACTS OF Prosopis juliflora Susceptibilidade Antimicrobiana de Bactérias Ocorrentes em Úlceras Crônicas aos Extratos Brutoss de Prosopis Juliflora |
title |
ANTIMICROBIAL SUSCEPTIBILIT Y OF BACTERIA IN CHRONIC ULCERS TO CRUDE EXTRACTS OF Prosopis juliflora |
spellingShingle |
ANTIMICROBIAL SUSCEPTIBILIT Y OF BACTERIA IN CHRONIC ULCERS TO CRUDE EXTRACTS OF Prosopis juliflora Silva, Renata Soares da Prosopis juliflora Úlceras crônicas Atividade antimicrobiana |
title_short |
ANTIMICROBIAL SUSCEPTIBILIT Y OF BACTERIA IN CHRONIC ULCERS TO CRUDE EXTRACTS OF Prosopis juliflora |
title_full |
ANTIMICROBIAL SUSCEPTIBILIT Y OF BACTERIA IN CHRONIC ULCERS TO CRUDE EXTRACTS OF Prosopis juliflora |
title_fullStr |
ANTIMICROBIAL SUSCEPTIBILIT Y OF BACTERIA IN CHRONIC ULCERS TO CRUDE EXTRACTS OF Prosopis juliflora |
title_full_unstemmed |
ANTIMICROBIAL SUSCEPTIBILIT Y OF BACTERIA IN CHRONIC ULCERS TO CRUDE EXTRACTS OF Prosopis juliflora |
title_sort |
ANTIMICROBIAL SUSCEPTIBILIT Y OF BACTERIA IN CHRONIC ULCERS TO CRUDE EXTRACTS OF Prosopis juliflora |
author |
Silva, Renata Soares da |
author_facet |
Silva, Renata Soares da Oliveira, Karla Maria Santos de Cavalcante, Giani Maria |
author_role |
author |
author2 |
Oliveira, Karla Maria Santos de Cavalcante, Giani Maria |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Silva, Renata Soares da Oliveira, Karla Maria Santos de Cavalcante, Giani Maria |
dc.subject.por.fl_str_mv |
Prosopis juliflora Úlceras crônicas Atividade antimicrobiana |
topic |
Prosopis juliflora Úlceras crônicas Atividade antimicrobiana |
description |
Chronic ulcers are prone to infection due to the sterile lossof the innate barrier of the skin and cutaneous annexes, coupled to the development of microbial communities. Current study investigates the susceptibility of bacteria associated with chronic ulcers to crude extracts of Prosopis juliflora. In vitro tests with the crude extracts of leaves and stem bark were performed against strains of Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 27853), Staphylococcus aureus (ATCC 25923) and Proteus mirabilis (ATCC 7002), by a diffusion assay in agar and determination of Minimum Inhibiory Concentration (MIC). Results showed that E. coli, P. aeruginosa, S. aureus and P.mirabilis strains were affected by the crude extracts of the leaves, with a diameter of the inhibition zone measuring respectively 21.0; 33.0; 20.0; 21.0 mm, whereas E. coli and S. aureus were more affected to the crude extracts of the stem bark, with an inhibition zone diameter of 21.5 and 20.0 mm, respectively. Best MIC rates were reported for the crude extracts of P. juliflora leaves. Chemical studies for the extraction and isolation of compounds are recommended for the execution of in vitro assays to investigate the compounds´ antimicrobial activity. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-12-31 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Avaliado por Pares |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://periodicos.unicesumar.edu.br/index.php/saudpesq/article/view/4488 10.17765/1983-1870.2015v8n3p493-499 |
url |
https://periodicos.unicesumar.edu.br/index.php/saudpesq/article/view/4488 |
identifier_str_mv |
10.17765/1983-1870.2015v8n3p493-499 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://periodicos.unicesumar.edu.br/index.php/saudpesq/article/view/4488/2697 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Cesumar - UniCesumar |
publisher.none.fl_str_mv |
Universidade Cesumar - UniCesumar |
dc.source.none.fl_str_mv |
Saúde e Pesquisa; Vol 8 No 3 (2015): set./dez.; 493-499 Saúde e Pesquisa; v. 8 n. 3 (2015): set./dez.; 493-499 2176-9206 reponame:Saúde e Pesquisa (Online) instname:Cesumar Diretoria de Pesquisa instacron:CESUMAR |
instname_str |
Cesumar Diretoria de Pesquisa |
instacron_str |
CESUMAR |
institution |
CESUMAR |
reponame_str |
Saúde e Pesquisa (Online) |
collection |
Saúde e Pesquisa (Online) |
repository.name.fl_str_mv |
Saúde e Pesquisa (Online) - Cesumar Diretoria de Pesquisa |
repository.mail.fl_str_mv |
naep@cesumar.br |
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1754122533543608320 |