ANTIMICROBIAL ACTIVITY OF VIOLACEIN AGAINST ORAL BACTERIA ASSOCIATED WITH HALITOSIS: AN IN VITRO STUDY
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Revista Científica do CRO-RJ (Online) |
Texto Completo: | https://cro-rj.org.br/revcientifica/index.php/revista/article/view/335 |
Resumo: | Introduction: violacein is a natural purple pigment produced by environmental bacteria that presents antimicrobial activity, particularly against Gram-positive bacteria. Intraoral halitosis (IOH) is a condition defined by the unpleasant odor emanating from the mouth, whose main source are volatile sulfur compounds, produced by Gram-negative oral bacteria on the tongue coating. In IOH treatment, antimicrobials have been indicated as chemical adjuncts, including natural products. Objective: thus, this study tested the antimicrobial activity of a violacein extract on key IOH-related bacteria (Porphyromonas gingivalis, Porphyromonas endodontalis, Fusobacterium nucleatum, Prevotella intermedia, Solobacterium moorei). Materials and Methods: bacteria were cultured in fastidious anaerobe blood agar in anaerobiosis, and 109 cells/ml suspensions were plated. Crude extract of violacein obtained from Chromobacterium violaceum was diluted in a 25% ethanol aqueous solution to 8, 4, 2, 1, 0.5 and 0.25 mg/ml. Using the disk agar diffusion method, 10 µl aliquots of each dilution were deposited on the seeded plates. Chlorohexidine (0.1%) and 25% ethanol solution were used as controls. Plates were incubated in anaerobiosis at 37°C for 72h, and the inhibition halos were recorded. Results: although chlorhexidine showed higher inhibition halos than the violacein extract, most species were inhibited at 4 and 8 mg/ml concentrations (p<0.05). P. gingivalis followed by F. nucleatum were the most affected species in relation to the other bacteria, although statistical significance was only reached for P. gingivalis (p<0.05). Conclusion: crude violacein extract from C. violaceum demonstrated antimicrobial activity against IOH-associated oral bacteria, being a potential antimicrobial to be studied as an adjunct in the control of IOH. |
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ANTIMICROBIAL ACTIVITY OF VIOLACEIN AGAINST ORAL BACTERIA ASSOCIATED WITH HALITOSIS: AN IN VITRO STUDYANTIMICROBIAL ACTIVITY OF VIOLACEIN AGAINST ORAL BACTERIA ASSOCIATED WITH HALITOSIS: AN IN VITRO STUDYHalitosisViolaceinVolatile Sulfur CompoundsOral PathogensHalitoseViolaceínaCompostos Voláteis de EnxofrePatógenos OraisIntroduction: violacein is a natural purple pigment produced by environmental bacteria that presents antimicrobial activity, particularly against Gram-positive bacteria. Intraoral halitosis (IOH) is a condition defined by the unpleasant odor emanating from the mouth, whose main source are volatile sulfur compounds, produced by Gram-negative oral bacteria on the tongue coating. In IOH treatment, antimicrobials have been indicated as chemical adjuncts, including natural products. Objective: thus, this study tested the antimicrobial activity of a violacein extract on key IOH-related bacteria (Porphyromonas gingivalis, Porphyromonas endodontalis, Fusobacterium nucleatum, Prevotella intermedia, Solobacterium moorei). Materials and Methods: bacteria were cultured in fastidious anaerobe blood agar in anaerobiosis, and 109 cells/ml suspensions were plated. Crude extract of violacein obtained from Chromobacterium violaceum was diluted in a 25% ethanol aqueous solution to 8, 4, 2, 1, 0.5 and 0.25 mg/ml. Using the disk agar diffusion method, 10 µl aliquots of each dilution were deposited on the seeded plates. Chlorohexidine (0.1%) and 25% ethanol solution were used as controls. Plates were incubated in anaerobiosis at 37°C for 72h, and the inhibition halos were recorded. Results: although chlorhexidine showed higher inhibition halos than the violacein extract, most species were inhibited at 4 and 8 mg/ml concentrations (p<0.05). P. gingivalis followed by F. nucleatum were the most affected species in relation to the other bacteria, although statistical significance was only reached for P. gingivalis (p<0.05). Conclusion: crude violacein extract from C. violaceum demonstrated antimicrobial activity against IOH-associated oral bacteria, being a potential antimicrobial to be studied as an adjunct in the control of IOH.Introdução: a violaceína é um pigmento roxo natural produzido por bactérias ambientais que apresenta ação antimicrobiana, particularmente contra bactérias Gram-positivas. A halitose intraoral (HIO) é uma condição definida pelo odor desagradável que emana da boca, cuja principal fonte são os compostos sulfurados voláteis produzidos por bactérias Gram-negativas da saburra lingual. No tratamento da HIO, antimicrobianos têm sido indicados como adjuvantes, incluindo produtos naturais. Objetivo: assim, este estudo avaliou o potencial antimicrobiano de um extrato de violaceína em patógenos-chave da HIO (Porphyromonas gingivalis, Porphyromonas endodontalis, Fusobacterium nucleatum, Prevotella intermedia, Solobacterium moorei). Materiais e Métodos: bactérias foram cultivadas em meio ágar sangue para fastidiosos, em anaerobiose, e suspensões de 109 células/ml foram semeadas. O extrato bruto de violaceína obtido de Chromobacterium violaceum foi diluído em solução aquosa com 25% de etanol nas concentrações de 8, 4, 2, 1, 0,5 e 0,25 mg/ml. Através do método de disco difusão, 10 µl de cada diluição foram depositados nas placas semeadas. A clorexidina (0,1%) e a solução etanólica a 25% foram usadas como controles. As placas foram incubadas em anaerobiose a 37°C por 72h, e os halos de inibição foram registrados. Resultados: embora a clorexidina tenha apresentado os maiores halos de inibição do do que o extrato, a maioria das espécies foi inibida nas concentrações de 4 e 8 mg/ml (p<0,05). P. gingivalis e F. nucleatum foram as espécies mais afetadas em relação às outras bactérias, porém só foi observada significância estatística para P. gingivalis (p<0,05). Conclusão: o extrato bruto de violaceína de C. violaceum demonstrou atividade antimicrobiana contra bactérias orais associadas a HIO, sendo um potencial antimicrobiano a ser estudado como adjuvante no controle da HIO.Rio de Janeiro Dental Journal2023-04-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://cro-rj.org.br/revcientifica/index.php/revista/article/view/33510.29327/244963.8.1-3Revista Científica do CRO-RJ (Rio de Janeiro Dental Journal); Vol. 8 No. 1: January-Abril 2023; 14-20Revista Científica do CRO-RJ (Rio de Janeiro Dental Journal); v. 8 n. 1: January-Abril 2023; 14-202595-47331518-524910.29327/244963.8.1reponame:Revista Científica do CRO-RJ (Online)instname:Conselho Regional de Odontologia do Rio de Janeiro (CRO-RJ)instacron:CROporhttps://cro-rj.org.br/revcientifica/index.php/revista/article/view/335/190Copyright (c) 2023 Revista Científica do CRO-RJ (Rio de Janeiro Dental Journal)http://creativecommons.org/licenses/by-nc-nd/4.0info:eu-repo/semantics/openAccessStephan Brooks, JayzonGomes Baeta Lourenço, TalitaSimas Cardoso Rurr, JanineVieira Colombo, Ana Paula2023-09-18T12:23:29Zoai:ojs3.cro-rj.org.br:article/335Revistahttps://cro-rj.org.br/revcientifica/index.php/revistahttps://cro-rj.org.br/revcientifica/index.php/revista/oairevista.cientifica@cro-rj.org.br || rorefa@terra.com.br2595-47331518-5249opendoar:2023-09-18T12:23:29Revista Científica do CRO-RJ (Online) - Conselho Regional de Odontologia do Rio de Janeiro (CRO-RJ)false |
dc.title.none.fl_str_mv |
ANTIMICROBIAL ACTIVITY OF VIOLACEIN AGAINST ORAL BACTERIA ASSOCIATED WITH HALITOSIS: AN IN VITRO STUDY ANTIMICROBIAL ACTIVITY OF VIOLACEIN AGAINST ORAL BACTERIA ASSOCIATED WITH HALITOSIS: AN IN VITRO STUDY |
title |
ANTIMICROBIAL ACTIVITY OF VIOLACEIN AGAINST ORAL BACTERIA ASSOCIATED WITH HALITOSIS: AN IN VITRO STUDY |
spellingShingle |
ANTIMICROBIAL ACTIVITY OF VIOLACEIN AGAINST ORAL BACTERIA ASSOCIATED WITH HALITOSIS: AN IN VITRO STUDY Stephan Brooks, Jayzon Halitosis Violacein Volatile Sulfur Compounds Oral Pathogens Halitose Violaceína Compostos Voláteis de Enxofre Patógenos Orais |
title_short |
ANTIMICROBIAL ACTIVITY OF VIOLACEIN AGAINST ORAL BACTERIA ASSOCIATED WITH HALITOSIS: AN IN VITRO STUDY |
title_full |
ANTIMICROBIAL ACTIVITY OF VIOLACEIN AGAINST ORAL BACTERIA ASSOCIATED WITH HALITOSIS: AN IN VITRO STUDY |
title_fullStr |
ANTIMICROBIAL ACTIVITY OF VIOLACEIN AGAINST ORAL BACTERIA ASSOCIATED WITH HALITOSIS: AN IN VITRO STUDY |
title_full_unstemmed |
ANTIMICROBIAL ACTIVITY OF VIOLACEIN AGAINST ORAL BACTERIA ASSOCIATED WITH HALITOSIS: AN IN VITRO STUDY |
title_sort |
ANTIMICROBIAL ACTIVITY OF VIOLACEIN AGAINST ORAL BACTERIA ASSOCIATED WITH HALITOSIS: AN IN VITRO STUDY |
author |
Stephan Brooks, Jayzon |
author_facet |
Stephan Brooks, Jayzon Gomes Baeta Lourenço, Talita Simas Cardoso Rurr, Janine Vieira Colombo, Ana Paula |
author_role |
author |
author2 |
Gomes Baeta Lourenço, Talita Simas Cardoso Rurr, Janine Vieira Colombo, Ana Paula |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Stephan Brooks, Jayzon Gomes Baeta Lourenço, Talita Simas Cardoso Rurr, Janine Vieira Colombo, Ana Paula |
dc.subject.por.fl_str_mv |
Halitosis Violacein Volatile Sulfur Compounds Oral Pathogens Halitose Violaceína Compostos Voláteis de Enxofre Patógenos Orais |
topic |
Halitosis Violacein Volatile Sulfur Compounds Oral Pathogens Halitose Violaceína Compostos Voláteis de Enxofre Patógenos Orais |
description |
Introduction: violacein is a natural purple pigment produced by environmental bacteria that presents antimicrobial activity, particularly against Gram-positive bacteria. Intraoral halitosis (IOH) is a condition defined by the unpleasant odor emanating from the mouth, whose main source are volatile sulfur compounds, produced by Gram-negative oral bacteria on the tongue coating. In IOH treatment, antimicrobials have been indicated as chemical adjuncts, including natural products. Objective: thus, this study tested the antimicrobial activity of a violacein extract on key IOH-related bacteria (Porphyromonas gingivalis, Porphyromonas endodontalis, Fusobacterium nucleatum, Prevotella intermedia, Solobacterium moorei). Materials and Methods: bacteria were cultured in fastidious anaerobe blood agar in anaerobiosis, and 109 cells/ml suspensions were plated. Crude extract of violacein obtained from Chromobacterium violaceum was diluted in a 25% ethanol aqueous solution to 8, 4, 2, 1, 0.5 and 0.25 mg/ml. Using the disk agar diffusion method, 10 µl aliquots of each dilution were deposited on the seeded plates. Chlorohexidine (0.1%) and 25% ethanol solution were used as controls. Plates were incubated in anaerobiosis at 37°C for 72h, and the inhibition halos were recorded. Results: although chlorhexidine showed higher inhibition halos than the violacein extract, most species were inhibited at 4 and 8 mg/ml concentrations (p<0.05). P. gingivalis followed by F. nucleatum were the most affected species in relation to the other bacteria, although statistical significance was only reached for P. gingivalis (p<0.05). Conclusion: crude violacein extract from C. violaceum demonstrated antimicrobial activity against IOH-associated oral bacteria, being a potential antimicrobial to be studied as an adjunct in the control of IOH. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-04-30 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://cro-rj.org.br/revcientifica/index.php/revista/article/view/335 10.29327/244963.8.1-3 |
url |
https://cro-rj.org.br/revcientifica/index.php/revista/article/view/335 |
identifier_str_mv |
10.29327/244963.8.1-3 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://cro-rj.org.br/revcientifica/index.php/revista/article/view/335/190 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2023 Revista Científica do CRO-RJ (Rio de Janeiro Dental Journal) http://creativecommons.org/licenses/by-nc-nd/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2023 Revista Científica do CRO-RJ (Rio de Janeiro Dental Journal) http://creativecommons.org/licenses/by-nc-nd/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Rio de Janeiro Dental Journal |
publisher.none.fl_str_mv |
Rio de Janeiro Dental Journal |
dc.source.none.fl_str_mv |
Revista Científica do CRO-RJ (Rio de Janeiro Dental Journal); Vol. 8 No. 1: January-Abril 2023; 14-20 Revista Científica do CRO-RJ (Rio de Janeiro Dental Journal); v. 8 n. 1: January-Abril 2023; 14-20 2595-4733 1518-5249 10.29327/244963.8.1 reponame:Revista Científica do CRO-RJ (Online) instname:Conselho Regional de Odontologia do Rio de Janeiro (CRO-RJ) instacron:CRO |
instname_str |
Conselho Regional de Odontologia do Rio de Janeiro (CRO-RJ) |
instacron_str |
CRO |
institution |
CRO |
reponame_str |
Revista Científica do CRO-RJ (Online) |
collection |
Revista Científica do CRO-RJ (Online) |
repository.name.fl_str_mv |
Revista Científica do CRO-RJ (Online) - Conselho Regional de Odontologia do Rio de Janeiro (CRO-RJ) |
repository.mail.fl_str_mv |
revista.cientifica@cro-rj.org.br || rorefa@terra.com.br |
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1797042305595604992 |