Major surface protein LipL32 is not required for either acute or chronic infection with Leptospira interrogans.

Detalhes bibliográficos
Autor(a) principal: Murray, Gerald L
Data de Publicação: 2009
Outros Autores: Srikram, Amporn, Hoke, David E, Wunder Junior, Elsio Augusto, Henry, Rebekah, Lo, Miranda, Zhang, Kunkun, Sermswan, Rasana W, Ko, Albert Icksang, Adler, Ben
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/12298
Resumo: Monash University. Australian Bacterial Pathogenesis Program / Australian Research Council Centre of Excellence in Structural. Clayton, Australia
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spelling Murray, Gerald LSrikram, AmpornHoke, David EWunder Junior, Elsio AugustoHenry, RebekahLo, MirandaZhang, KunkunSermswan, Rasana WKo, Albert IcksangAdler, Ben2015-11-20T14:24:16Z2015-11-20T14:24:16Z2009MURRAY, G. L. et al. Major surface protein LipL32 is not required for either acute or chronic infection with Leptospira interrogans. Infection and Immunity, v. 77, n. 3, p. 952-958, 2009.1098-5522https://www.arca.fiocruz.br/handle/icict/1229810.1128/IAI.01370-08engAmerican Society of MicrobiologyMajor surface protein LipL32 is not required for either acute or chronic infection with Leptospira interrogans.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleMonash University. Australian Bacterial Pathogenesis Program / Australian Research Council Centre of Excellence in Structural. Clayton, AustraliaKhon Kaen University. Faculty of Medicine. Melioidosis Research Center. Department of Biochemistry. Khon Kaen, ThailandMonash University. Australian Bacterial Pathogenesis Program / Australian Research Council Centre of Excellence in Structural. Clayton, AustraliaFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilMonash University. Australian Bacterial Pathogenesis Program / Australian Research Council Centre of Excellence in Structural. Clayton, AustraliaMonash University. Australian Bacterial Pathogenesis Program / Australian Research Council Centre of Excellence in Structural. Clayton, AustraliaMonash University. Functional Microbial Genomics. Department of Microbiology. Clayton, AustraliaKhon Kaen University. Faculty of Medicine. Khon Kaen, ThailandFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Weill Medical College of Cornell University. Division of International Medicine and Infectious Disease. New York, New YorkMonash University. Australian Bacterial Pathogenesis Program / Australian Research Council Centre of Excellence in Structural. Clayton, Australia / Monash University. Functional Microbial Genomics. Department of Microbiology. Clayton, AustraliaLeptospira interrogans is responsible for leptospirosis, a zoonosis of worldwide distribution. LipL32 is the major outer membrane protein of pathogenic leptospires, accounting for up to 75% of total outer membrane protein. In recent times LipL32 has become the focus of intense study because of its surface location, dominance in the host immune response, and conservation among pathogenic species. In this study, an lipL32 mutant was constructed in L. interrogans using transposon mutagenesis. The lipL32 mutant had normal morphology and growth rate compared to the wild type and was equally adherent to extracellular matrix. Protein composition of the cell membranes was found to be largely unaffected by the loss of LipL32, with no obvious compensatory increase in other proteins. Microarray studies found no obvious stress response or upregulation of genes that may compensate for the loss of LipL32 but did suggest an association between LipL32 and the synthesis of heme and vitamin B12. When hamsters were inoculated by systemic and mucosal routes, the mutant caused acute severe disease manifestations that were indistinguishable from wild-type L. interrogans infection. In the rat model of chronic infection, the LipL32 mutant colonized the renal tubules as efficiently as the wild-type strain. In conclusion, this study showed that LipL32 does not play a role in either the acute or chronic models of infection. Considering the abundance and conservation of LipL32 among all pathogenic Leptospira spp. and its absence in saprophytic Leptospira, this finding is remarkable. The role of this protein in leptospiral biology and pathogenesis thus remains elusive.Proteínas da Membrana Bacteriana Externa/metabolismoLeptospira interrogans/patogenicidadeLeptospirose/metabolismoLipoproteínas/metabolismoAnimaisProteínas da Membrana Bacteriana Externa/genéticaWestern BlottingCricetinaeLeptospira interrogans/genéticaLeptospira interrogans/metabolismoLeptospirose/genéticaMesocricetusAnálise de Sequência com Séries de OligonucleotídeosReação em Cadeia da PolimeraseRatosRatos Wistarinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/12298/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALMurray GL and major surface protein...pdfMurray GL and major surface 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dc.title.pt_BR.fl_str_mv Major surface protein LipL32 is not required for either acute or chronic infection with Leptospira interrogans.
title Major surface protein LipL32 is not required for either acute or chronic infection with Leptospira interrogans.
spellingShingle Major surface protein LipL32 is not required for either acute or chronic infection with Leptospira interrogans.
Murray, Gerald L
Proteínas da Membrana Bacteriana Externa/metabolismo
Leptospira interrogans/patogenicidade
Leptospirose/metabolismo
Lipoproteínas/metabolismo
Animais
Proteínas da Membrana Bacteriana Externa/genética
Western Blotting
Cricetinae
Leptospira interrogans/genética
Leptospira interrogans/metabolismo
Leptospirose/genética
Mesocricetus
Análise de Sequência com Séries de Oligonucleotídeos
Reação em Cadeia da Polimerase
Ratos
Ratos Wistar
title_short Major surface protein LipL32 is not required for either acute or chronic infection with Leptospira interrogans.
title_full Major surface protein LipL32 is not required for either acute or chronic infection with Leptospira interrogans.
title_fullStr Major surface protein LipL32 is not required for either acute or chronic infection with Leptospira interrogans.
title_full_unstemmed Major surface protein LipL32 is not required for either acute or chronic infection with Leptospira interrogans.
title_sort Major surface protein LipL32 is not required for either acute or chronic infection with Leptospira interrogans.
author Murray, Gerald L
author_facet Murray, Gerald L
Srikram, Amporn
Hoke, David E
Wunder Junior, Elsio Augusto
Henry, Rebekah
Lo, Miranda
Zhang, Kunkun
Sermswan, Rasana W
Ko, Albert Icksang
Adler, Ben
author_role author
author2 Srikram, Amporn
Hoke, David E
Wunder Junior, Elsio Augusto
Henry, Rebekah
Lo, Miranda
Zhang, Kunkun
Sermswan, Rasana W
Ko, Albert Icksang
Adler, Ben
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Murray, Gerald L
Srikram, Amporn
Hoke, David E
Wunder Junior, Elsio Augusto
Henry, Rebekah
Lo, Miranda
Zhang, Kunkun
Sermswan, Rasana W
Ko, Albert Icksang
Adler, Ben
dc.subject.decs.pt_BR.fl_str_mv Proteínas da Membrana Bacteriana Externa/metabolismo
Leptospira interrogans/patogenicidade
Leptospirose/metabolismo
Lipoproteínas/metabolismo
Animais
Proteínas da Membrana Bacteriana Externa/genética
Western Blotting
Cricetinae
Leptospira interrogans/genética
Leptospira interrogans/metabolismo
Leptospirose/genética
Mesocricetus
Análise de Sequência com Séries de Oligonucleotídeos
Reação em Cadeia da Polimerase
Ratos
Ratos Wistar
topic Proteínas da Membrana Bacteriana Externa/metabolismo
Leptospira interrogans/patogenicidade
Leptospirose/metabolismo
Lipoproteínas/metabolismo
Animais
Proteínas da Membrana Bacteriana Externa/genética
Western Blotting
Cricetinae
Leptospira interrogans/genética
Leptospira interrogans/metabolismo
Leptospirose/genética
Mesocricetus
Análise de Sequência com Séries de Oligonucleotídeos
Reação em Cadeia da Polimerase
Ratos
Ratos Wistar
description Monash University. Australian Bacterial Pathogenesis Program / Australian Research Council Centre of Excellence in Structural. Clayton, Australia
publishDate 2009
dc.date.issued.fl_str_mv 2009
dc.date.accessioned.fl_str_mv 2015-11-20T14:24:16Z
dc.date.available.fl_str_mv 2015-11-20T14:24:16Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MURRAY, G. L. et al. Major surface protein LipL32 is not required for either acute or chronic infection with Leptospira interrogans. Infection and Immunity, v. 77, n. 3, p. 952-958, 2009.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/12298
dc.identifier.issn.none.fl_str_mv 1098-5522
dc.identifier.doi.none.fl_str_mv 10.1128/IAI.01370-08
identifier_str_mv MURRAY, G. L. et al. Major surface protein LipL32 is not required for either acute or chronic infection with Leptospira interrogans. Infection and Immunity, v. 77, n. 3, p. 952-958, 2009.
1098-5522
10.1128/IAI.01370-08
url https://www.arca.fiocruz.br/handle/icict/12298
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Society of Microbiology
publisher.none.fl_str_mv American Society of Microbiology
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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