Sera from Visceral Leishmaniasis Patients Display Oxidative Activity and Affect the TNF-αProduction by MacrophagesIn Vitro

Detalhes bibliográficos
Autor(a) principal: Soares, Neci Matos
Data de Publicação: 2017
Outros Autores: Souza, Joelma Nascimento de, Leal, Tatiana F, Reis, Eliana Almeida Gomes, Miranda, Maria da P. Spinola, dosSantos, Washington Luis Conrado, Teixeira, Márcia Cristina Aquino
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/25647
Resumo: Universidade Federal da Bahia. Faculdade de Farmácia. Departamento de Análises Clínicas e Toxicológicas. Salvador, BA, Brasil
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spelling Soares, Neci MatosSouza, Joelma Nascimento deLeal, Tatiana FReis, Eliana Almeida GomesMiranda, Maria da P. SpinoladosSantos, Washington Luis ConradoTeixeira, Márcia Cristina Aquino2018-04-05T18:11:20Z2018-04-05T18:11:20Z2017SPARES, N. M. et al. Sera from Visceral Leishmaniasis Patients Display Oxidative Activity and Affect the TNF-αProduction by MacrophagesIn Vitro. BioMed Research International, ID 5861453, 2017.2314-6133https://www.arca.fiocruz.br/handle/icict/2564710.1155/2017/5861453engHindawi Publishing CorporationLeishmaniose visceralSoroAtivação de macrofagosHumanosVisceral LeishmaniasisSeraMacrophages activationHumansSera from Visceral Leishmaniasis Patients Display Oxidative Activity and Affect the TNF-αProduction by MacrophagesIn Vitroinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal da Bahia. Faculdade de Farmácia. Departamento de Análises Clínicas e Toxicológicas. Salvador, BA, BrasilUniversidade Federal da Bahia. Faculdade de Farmácia. Departamento de Análises Clínicas e Toxicológicas. Salvador, BA, BrasilUniversidade Federal da Bahia. Faculdade de Farmácia. Departamento de Análises Clínicas e Toxicológicas. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal da Bahia. Faculdade de Farmácia. Departamento de Análises Clínicas e Toxicológicas. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal da Bahia. Faculdade de Farmácia. Departamento de Análises Clínicas e Toxicológicas. Salvador, BA, BrasilMammalian protection against leishmanial infection depends on the development of an effective immune response. Zoonotic visceral leishmaniasis (ZVL) patients are usually unable to mount an effective immune response against the parasite and indeed appear to be severely immunosuppressed. This suppression has strong nonspecific and specific components mediated by serum factors and leishmanicidal activity of infected macrophages, respectively. The lipid profile has been shown to be altered in ZVL patients' sera. This work aimed at (i) determining the HDL, Apo A1, LDL, and VLDL concentrations in ZVL patients' sera; (ii) investigating the oxidative effect of ZVL patients' sera on theβ-carotene matrix; (iii) measuring IL-10, IL-6, IL-12p40, and tumour necrosis factor-α(TNF-α) concentrations in the macrophage cultures, to which 10% of ZVL patients' serum had been added. Levels of HDL, LDL fraction, and apolipoprotein A1 in ZVL patients' sera were lower than those of healthy individuals' sera, except for the mean level of VLDL. The matrix ofβ-carotene and linoleic acid system was oxidized in the presence of ZLV patients' sera. The presence of ZVL patients' sera did not modify the cytokine production of IL-6, IL-12p40, and IL-10 by human macrophagesin vitrobut TNF-αproduction was altered, probably due to lack of macrophage stimulation by lipoprotein.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/25647/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALSoares NM Sera from Visceral Leishmaniasis Patients....pdfSoares NM Sera from Visceral Leishmaniasis Patients....pdfapplication/pdf712177https://www.arca.fiocruz.br/bitstream/icict/25647/2/Soares%20NM%20Sera%20from%20Visceral%20Leishmaniasis%20Patients....pdfd58fde5167450c1a9b12e7e1afe5c0d2MD52TEXTSoares NM Sera from Visceral Leishmaniasis Patients....pdf.txtSoares NM Sera from Visceral Leishmaniasis Patients....pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Sera from Visceral Leishmaniasis Patients Display Oxidative Activity and Affect the TNF-αProduction by MacrophagesIn Vitro
title Sera from Visceral Leishmaniasis Patients Display Oxidative Activity and Affect the TNF-αProduction by MacrophagesIn Vitro
spellingShingle Sera from Visceral Leishmaniasis Patients Display Oxidative Activity and Affect the TNF-αProduction by MacrophagesIn Vitro
Soares, Neci Matos
Leishmaniose visceral
Soro
Ativação de macrofagos
Humanos
Visceral Leishmaniasis
Sera
Macrophages activation
Humans
title_short Sera from Visceral Leishmaniasis Patients Display Oxidative Activity and Affect the TNF-αProduction by MacrophagesIn Vitro
title_full Sera from Visceral Leishmaniasis Patients Display Oxidative Activity and Affect the TNF-αProduction by MacrophagesIn Vitro
title_fullStr Sera from Visceral Leishmaniasis Patients Display Oxidative Activity and Affect the TNF-αProduction by MacrophagesIn Vitro
title_full_unstemmed Sera from Visceral Leishmaniasis Patients Display Oxidative Activity and Affect the TNF-αProduction by MacrophagesIn Vitro
title_sort Sera from Visceral Leishmaniasis Patients Display Oxidative Activity and Affect the TNF-αProduction by MacrophagesIn Vitro
author Soares, Neci Matos
author_facet Soares, Neci Matos
Souza, Joelma Nascimento de
Leal, Tatiana F
Reis, Eliana Almeida Gomes
Miranda, Maria da P. Spinola
dosSantos, Washington Luis Conrado
Teixeira, Márcia Cristina Aquino
author_role author
author2 Souza, Joelma Nascimento de
Leal, Tatiana F
Reis, Eliana Almeida Gomes
Miranda, Maria da P. Spinola
dosSantos, Washington Luis Conrado
Teixeira, Márcia Cristina Aquino
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Soares, Neci Matos
Souza, Joelma Nascimento de
Leal, Tatiana F
Reis, Eliana Almeida Gomes
Miranda, Maria da P. Spinola
dosSantos, Washington Luis Conrado
Teixeira, Márcia Cristina Aquino
dc.subject.other.pt_BR.fl_str_mv Leishmaniose visceral
Soro
Ativação de macrofagos
Humanos
topic Leishmaniose visceral
Soro
Ativação de macrofagos
Humanos
Visceral Leishmaniasis
Sera
Macrophages activation
Humans
dc.subject.en.pt_BR.fl_str_mv Visceral Leishmaniasis
Sera
Macrophages activation
Humans
description Universidade Federal da Bahia. Faculdade de Farmácia. Departamento de Análises Clínicas e Toxicológicas. Salvador, BA, Brasil
publishDate 2017
dc.date.issued.fl_str_mv 2017
dc.date.accessioned.fl_str_mv 2018-04-05T18:11:20Z
dc.date.available.fl_str_mv 2018-04-05T18:11:20Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SPARES, N. M. et al. Sera from Visceral Leishmaniasis Patients Display Oxidative Activity and Affect the TNF-αProduction by MacrophagesIn Vitro. BioMed Research International, ID 5861453, 2017.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/25647
dc.identifier.issn.pt_BR.fl_str_mv 2314-6133
dc.identifier.doi.none.fl_str_mv 10.1155/2017/5861453
identifier_str_mv SPARES, N. M. et al. Sera from Visceral Leishmaniasis Patients Display Oxidative Activity and Affect the TNF-αProduction by MacrophagesIn Vitro. BioMed Research International, ID 5861453, 2017.
2314-6133
10.1155/2017/5861453
url https://www.arca.fiocruz.br/handle/icict/25647
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Hindawi Publishing Corporation
publisher.none.fl_str_mv Hindawi Publishing Corporation
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/25647/1/license.txt
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