Bacterial Clearance Is Improved in Septic Mice by Platelet-Activating Factor-Acetylhydrolase (PAF-AH) Administration
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2013 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/12598 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil. |
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Cunha, Mariana G. A. Teixeira daGomes, Rachel N.Roehrs, NathassiaBozza, Fernando A.Prescott, Stephen M.Stafforini, DianaZimmerman, Guy A.Bozza, Patrícia T.Faria Neto, Hugo C. Castro2016-01-22T17:06:17Z2016-01-22T17:06:17Z2013CUNHA, Mariana G. A. Teixeira da; Bacterial Clearance Is Improved in Septic Mice by Platelet-Activating Factor-Acetylhydrolase (PAF-AH) Administration. Plos One, v.8, n.9, e74567. 7p, 2013.1932-6203https://www.arca.fiocruz.br/handle/icict/1259810.1371/journal.pone.0074567engPublic Library of ScienceBacterial Clearance Is Improved in Septic Mice by Platelet-Activating Factor-Acetylhydrolase (PAF-AH) Administrationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Investigação em Medicina Intensiva. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Investigação em Medicina Intensiva. Rio de Janeiro, RJ, BrasilOklahoma Medical Research Foundation. Oklahoma City, USA.Huntsman Cancer Institute. Salt Lake City, Utah, USA.University of Utah. Program in Human Molecular Biology and Genetics. Salt Lake City, Utah, USA.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Current evidence indicates that dysregulation of the host inflammatory response to infectious agents is central to the mortality of patients with sepsis. Strategies to block inflammatory mediators such as PAF have been investigated as adjuvant therapies for sepsis. PAF-AH, the enzyme responsible for PAF degradation, showed positive results in pre-clinical studies and phase II clinical trials, but the results of a phase III study were disappointing. In this study, we investigated the potential protective mechanism of PAF-AH in sepsis using the murine model of cecal ligation and puncture (CLP). Treatment with rPAF-AH increased peritoneal fluid levels of the anti-inflammatory mediators MCP-1/CCL2 after CLP. The numbers of bacteria (CFU) in the peritoneal cavity were decreased in the rPAF-AH-treated group, indicating more efficient bacterial clearance after rPAF-AH treatment. Interestingly, we observed increased levels of nitric oxide (NO) after PAF-AH administration, and rPAF-AH treatment did not decrease CFU numbers either in iNOS-deficient mice or in CCR2-deficient mice. We concluded that administration of exogenous rPAF-AH reduced inflammatory injury, altered cytokine levels and favored bacterial clearance with a clear impact on mortality through modulation of MCP-1/CCL2 and NO levels in a clinically relevant sepsis model.Platelet-Activating Factor-Acetylhydrolase (PAF-AH)SepsisSepseinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/12598/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALmariana_cunha_etal_IOC_2013.pdfmariana_cunha_etal_IOC_2013.pdfapplication/pdf372931https://www.arca.fiocruz.br/bitstream/icict/12598/2/mariana_cunha_etal_IOC_2013.pdfa0f16ff6e7e6a14b1b200ed3a86cb61eMD52TEXTmariana_cunha_etal_IOC_2013.pdf.txtmariana_cunha_etal_IOC_2013.pdf.txtExtracted 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| dc.title.pt_BR.fl_str_mv |
Bacterial Clearance Is Improved in Septic Mice by Platelet-Activating Factor-Acetylhydrolase (PAF-AH) Administration |
| title |
Bacterial Clearance Is Improved in Septic Mice by Platelet-Activating Factor-Acetylhydrolase (PAF-AH) Administration |
| spellingShingle |
Bacterial Clearance Is Improved in Septic Mice by Platelet-Activating Factor-Acetylhydrolase (PAF-AH) Administration Cunha, Mariana G. A. Teixeira da Platelet-Activating Factor-Acetylhydrolase (PAF-AH) Sepsis Sepse |
| title_short |
Bacterial Clearance Is Improved in Septic Mice by Platelet-Activating Factor-Acetylhydrolase (PAF-AH) Administration |
| title_full |
Bacterial Clearance Is Improved in Septic Mice by Platelet-Activating Factor-Acetylhydrolase (PAF-AH) Administration |
| title_fullStr |
Bacterial Clearance Is Improved in Septic Mice by Platelet-Activating Factor-Acetylhydrolase (PAF-AH) Administration |
| title_full_unstemmed |
Bacterial Clearance Is Improved in Septic Mice by Platelet-Activating Factor-Acetylhydrolase (PAF-AH) Administration |
| title_sort |
Bacterial Clearance Is Improved in Septic Mice by Platelet-Activating Factor-Acetylhydrolase (PAF-AH) Administration |
| author |
Cunha, Mariana G. A. Teixeira da |
| author_facet |
Cunha, Mariana G. A. Teixeira da Gomes, Rachel N. Roehrs, Nathassia Bozza, Fernando A. Prescott, Stephen M. Stafforini, Diana Zimmerman, Guy A. Bozza, Patrícia T. Faria Neto, Hugo C. Castro |
| author_role |
author |
| author2 |
Gomes, Rachel N. Roehrs, Nathassia Bozza, Fernando A. Prescott, Stephen M. Stafforini, Diana Zimmerman, Guy A. Bozza, Patrícia T. Faria Neto, Hugo C. Castro |
| author2_role |
author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Cunha, Mariana G. A. Teixeira da Gomes, Rachel N. Roehrs, Nathassia Bozza, Fernando A. Prescott, Stephen M. Stafforini, Diana Zimmerman, Guy A. Bozza, Patrícia T. Faria Neto, Hugo C. Castro |
| dc.subject.en.pt_BR.fl_str_mv |
Platelet-Activating Factor-Acetylhydrolase (PAF-AH) Sepsis |
| topic |
Platelet-Activating Factor-Acetylhydrolase (PAF-AH) Sepsis Sepse |
| dc.subject.decs.pt_BR.fl_str_mv |
Sepse |
| description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil. |
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2013 |
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2013 |
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2016-01-22T17:06:17Z |
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2016-01-22T17:06:17Z |
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CUNHA, Mariana G. A. Teixeira da; Bacterial Clearance Is Improved in Septic Mice by Platelet-Activating Factor-Acetylhydrolase (PAF-AH) Administration. Plos One, v.8, n.9, e74567. 7p, 2013. |
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https://www.arca.fiocruz.br/handle/icict/12598 |
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1932-6203 |
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10.1371/journal.pone.0074567 |
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CUNHA, Mariana G. A. Teixeira da; Bacterial Clearance Is Improved in Septic Mice by Platelet-Activating Factor-Acetylhydrolase (PAF-AH) Administration. Plos One, v.8, n.9, e74567. 7p, 2013. 1932-6203 10.1371/journal.pone.0074567 |
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