Human immunodeficiency virus type 1 (HIV-1) genotyping in Rio de Janeiro, Brazil: assessing subtype and drug-resistance associated mutations in HIV-1 infected individuals failing highly active antiretroviral therapy
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2005 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/31263 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ, Brasil. |
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Couto-Fernandez, J. C.Silva-de-Jesus, C.Veloso, Valdiléa G.Rachid, M.Gracie, R. S. G.Chequer-Fernandez, S. L.Oliveira, S. M.Arakaki-Sanchez, D.Chequer, P. J. N.Morgado, M. G.2019-01-23T16:52:51Z2019-01-23T16:52:51Z2005COUTO-FERNANDEZ, J. C. et al. Human immunodeficiency virus type 1 (HIV-1) genotyping in Rio de Janeiro, Brazil: assessing subtype and drug-resistance associated mutations in HIV-1 infected individuals failing highly active antiretroviral therapy. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 100, n. 1, p. 73-78, 2005.0074-0276https://www.arca.fiocruz.br/handle/icict/3126310.1590/S0074-02762005000100014engInstituto Oswaldo CruzVírus da imunodeficiência humana tipo 1SubtiposGenotipagemResistênciaBrasilHuman immunodeficiency virus type 1SubtypesGenotypingResistanceBrazilHuman immunodeficiency virus type 1 (HIV-1) genotyping in Rio de Janeiro, Brazil: assessing subtype and drug-resistance associated mutations in HIV-1 infected individuals failing highly active antiretroviral therapyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em DST/AIDS. Rio de Janeiro, RJ, Brasil.Secretaria Estadual de Saúde do Rio de Janeiro. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Geoprocessamento-DIS/CIC. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Geoprocessamento-DIS/CIC. Rio de Janeiro, RJ, Brasil.Ministério da Saúde. Programa Nacional de DST e AIDS. Brasília, DF, Brasil.Ministério da Saúde. Programa Nacional de DST e AIDS. Brasília, DF, Brasil.Ministério da Saúde. Programa Nacional de DST e AIDS. Brasília, DF, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ, Brasil.In order to assess the human immunodeficiency virus type 1 (HIV-1) drug resistance mutation profiles and evaluate the distribution of the genetic subtypes in the state of Rio de Janeiro, Brazil, blood samples from 547 HIV-1 infected patients failing antiretroviral (ARV) therapy, were collected during the years 2002 and 2003 to perform the viral resistance genotyping at the Renageno Laboratory from Rio de Janeiro (Oswaldo Cruz Foundation). Viral resistance genotyping was performed using ViroSeqTM Genotyping System (Celera Diagnostic-Abbott, US). The HIV-1 subtyping based on polymerase (pol) gene sequences (protease and reverse transcriptase-RT regions) was as follows: subtype B (91.2%), subtype F (4.9%), and B/F viral recombinant forms (3.3%). The subtype C was identified in two patients (0.4%) and the recombinant CRF_02/AG virus was found infecting one patient (0.2%). The HIV-1 genotyping profile associated to the reverse transcriptase inhibitors has shown a high frequency of the M184V mutation followed by the timidine-associated mutations. The K103N mutation was the most prevalent to the non-nucleoside RT inhibitor and the resistance associated to protease inhibitor showed the minor mutations L63P, L10F/R, and A71V as the more prevalent. A large proportion of subtype B was observed in HIV-1 treated patients from Rio de Janeiro. In addition, we have identified the circulation of drug-resistant HIV-1 subtype C and are presenting the first report of the occurrence of an African recombinant CRF_02/AG virus in Rio de Janeiro, Brazil. A clear association between HIV-1 subtypes and protease resistance mutations was observed in this study. The maintenance of resistance genotyping programs for HIV-1 failing patients is important to the management of ARV therapies and to attempt and monitor the HIV-1 subtype prevalence in Brazil.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83099https://www.arca.fiocruz.br/bitstream/icict/31263/1/license.txt586c046dcfeef936e32f0323bb9a47c0MD51ORIGINALve_Valdilea_Veloso_etal_INI_Lapclin-AIDS_2005.pdfve_Valdilea_Veloso_etal_INI_Lapclin-AIDS_2005.pdfapplication/pdf565096https://www.arca.fiocruz.br/bitstream/icict/31263/2/ve_Valdilea_Veloso_etal_INI_Lapclin-AIDS_2005.pdfb48e343e53af68bf5d3ee93a3ddfdb39MD52TEXTve_Valdilea_Veloso_etal_INI_Lapclin-AIDS_2005.pdf.txtve_Valdilea_Veloso_etal_INI_Lapclin-AIDS_2005.pdf.txtExtracted 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Human immunodeficiency virus type 1 (HIV-1) genotyping in Rio de Janeiro, Brazil: assessing subtype and drug-resistance associated mutations in HIV-1 infected individuals failing highly active antiretroviral therapy |
| title |
Human immunodeficiency virus type 1 (HIV-1) genotyping in Rio de Janeiro, Brazil: assessing subtype and drug-resistance associated mutations in HIV-1 infected individuals failing highly active antiretroviral therapy |
| spellingShingle |
Human immunodeficiency virus type 1 (HIV-1) genotyping in Rio de Janeiro, Brazil: assessing subtype and drug-resistance associated mutations in HIV-1 infected individuals failing highly active antiretroviral therapy Couto-Fernandez, J. C. Vírus da imunodeficiência humana tipo 1 Subtipos Genotipagem Resistência Brasil Human immunodeficiency virus type 1 Subtypes Genotyping Resistance Brazil |
| title_short |
Human immunodeficiency virus type 1 (HIV-1) genotyping in Rio de Janeiro, Brazil: assessing subtype and drug-resistance associated mutations in HIV-1 infected individuals failing highly active antiretroviral therapy |
| title_full |
Human immunodeficiency virus type 1 (HIV-1) genotyping in Rio de Janeiro, Brazil: assessing subtype and drug-resistance associated mutations in HIV-1 infected individuals failing highly active antiretroviral therapy |
| title_fullStr |
Human immunodeficiency virus type 1 (HIV-1) genotyping in Rio de Janeiro, Brazil: assessing subtype and drug-resistance associated mutations in HIV-1 infected individuals failing highly active antiretroviral therapy |
| title_full_unstemmed |
Human immunodeficiency virus type 1 (HIV-1) genotyping in Rio de Janeiro, Brazil: assessing subtype and drug-resistance associated mutations in HIV-1 infected individuals failing highly active antiretroviral therapy |
| title_sort |
Human immunodeficiency virus type 1 (HIV-1) genotyping in Rio de Janeiro, Brazil: assessing subtype and drug-resistance associated mutations in HIV-1 infected individuals failing highly active antiretroviral therapy |
| author |
Couto-Fernandez, J. C. |
| author_facet |
Couto-Fernandez, J. C. Silva-de-Jesus, C. Veloso, Valdiléa G. Rachid, M. Gracie, R. S. G. Chequer-Fernandez, S. L. Oliveira, S. M. Arakaki-Sanchez, D. Chequer, P. J. N. Morgado, M. G. |
| author_role |
author |
| author2 |
Silva-de-Jesus, C. Veloso, Valdiléa G. Rachid, M. Gracie, R. S. G. Chequer-Fernandez, S. L. Oliveira, S. M. Arakaki-Sanchez, D. Chequer, P. J. N. Morgado, M. G. |
| author2_role |
author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Couto-Fernandez, J. C. Silva-de-Jesus, C. Veloso, Valdiléa G. Rachid, M. Gracie, R. S. G. Chequer-Fernandez, S. L. Oliveira, S. M. Arakaki-Sanchez, D. Chequer, P. J. N. Morgado, M. G. |
| dc.subject.other.pt_BR.fl_str_mv |
Vírus da imunodeficiência humana tipo 1 Subtipos Genotipagem Resistência Brasil |
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Vírus da imunodeficiência humana tipo 1 Subtipos Genotipagem Resistência Brasil Human immunodeficiency virus type 1 Subtypes Genotyping Resistance Brazil |
| dc.subject.en.pt_BR.fl_str_mv |
Human immunodeficiency virus type 1 Subtypes Genotyping Resistance Brazil |
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Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ, Brasil. |
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2005 |
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2005 |
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2019-01-23T16:52:51Z |
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COUTO-FERNANDEZ, J. C. et al. Human immunodeficiency virus type 1 (HIV-1) genotyping in Rio de Janeiro, Brazil: assessing subtype and drug-resistance associated mutations in HIV-1 infected individuals failing highly active antiretroviral therapy. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 100, n. 1, p. 73-78, 2005. |
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https://www.arca.fiocruz.br/handle/icict/31263 |
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0074-0276 |
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10.1590/S0074-02762005000100014 |
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COUTO-FERNANDEZ, J. C. et al. Human immunodeficiency virus type 1 (HIV-1) genotyping in Rio de Janeiro, Brazil: assessing subtype and drug-resistance associated mutations in HIV-1 infected individuals failing highly active antiretroviral therapy. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 100, n. 1, p. 73-78, 2005. 0074-0276 10.1590/S0074-02762005000100014 |
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