Whole blood assay to access T Cell-immune responses to Mycobacterium tuberculosis antigens in Healthy Brazilian individuals
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2004 |
| Outros Autores: | , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/41445 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. |
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Antas, Paulo R. Z.Cardoso, Fernando L. L.Oliveira, Eliane B.Gomes, Patrícia K. C.Cunha, Kátia S.Sarno, Euzenir N.Sampaio, Elizabeth P.2020-05-29T13:11:28Z2020-05-29T13:11:28Z2004ANTAS, Paulo R. Z. et al. Whole Blood Assay to Access T Cell-immune responses to Mycobacterium tuberculosis antigens in Health Brazilian individuals. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 99, n. 1, p. 53-55, Feb. 2004.0074-0276https://www.arca.fiocruz.br/handle/icict/4144510.1590/S0074-027620040001000091678-8060engFundação Oswaldo Cruz. Instituto Oswaldo CruzTuberculosePPDInfecção latente de TubereculoseInterferon yRespostas de Células TTuberculosisT cell-responsesWhole bloodPPDLatent TB infectionsWhole blood assay to access T Cell-immune responses to Mycobacterium tuberculosis antigens in Healthy Brazilian individualsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseíase. Rio de Janeiro, RJ, Brasil.Hospital Municipal Raphael de Paula e Souza. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inovações em Terapias, Ensino e Bioprodutos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inovações em Terapias, Ensino e Bioprodutos. Rio de Janeiro, RJ, Brasil.The production of interferon γ (IFNγ) guarantees effective T cell-mediated immunity against Mycobacterium tuberculosis infection. In the present study, we simply compare the in vitro immune responses to Mycobacterium antigens in terms of IFNγ production in a total of 10 healthy Brazilian volunteers. Whole blood and mononuclear cells were cultivated in parallel with PPD, Ag85B, and M. bovis hsp65, and five-days supernatants were harvested for cytokine detection by ELISA. The inter-assay result was that the overall profile of agreement in response to antigens was highly correlated (r2 = 0.9266; p = 0.0102). Potential analysis is in current progress to dictate the usefulness of this method to access the immune responses also in tuberculosis patients and its contacts.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/41445/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALPauloRZ_Antas_etal_IOC_2004.pdfPauloRZ_Antas_etal_IOC_2004.pdfapplication/pdf54766https://www.arca.fiocruz.br/bitstream/icict/41445/2/PauloRZ_Antas_etal_IOC_2004.pdfd3de7cb243ab6c5a1a0d6239a58eeba9MD52TEXTPauloRZ_Antas_etal_IOC_2004.pdf.txtPauloRZ_Antas_etal_IOC_2004.pdf.txtExtracted texttext/plain12036https://www.arca.fiocruz.br/bitstream/icict/41445/3/PauloRZ_Antas_etal_IOC_2004.pdf.txt86a6cb914258e0bdc64a204e5513a741MD53icict/414452020-05-30 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| dc.title.pt_BR.fl_str_mv |
Whole blood assay to access T Cell-immune responses to Mycobacterium tuberculosis antigens in Healthy Brazilian individuals |
| title |
Whole blood assay to access T Cell-immune responses to Mycobacterium tuberculosis antigens in Healthy Brazilian individuals |
| spellingShingle |
Whole blood assay to access T Cell-immune responses to Mycobacterium tuberculosis antigens in Healthy Brazilian individuals Antas, Paulo R. Z. Tuberculose PPD Infecção latente de Tubereculose Interferon y Respostas de Células T Tuberculosis T cell-responses Whole blood PPD Latent TB infections |
| title_short |
Whole blood assay to access T Cell-immune responses to Mycobacterium tuberculosis antigens in Healthy Brazilian individuals |
| title_full |
Whole blood assay to access T Cell-immune responses to Mycobacterium tuberculosis antigens in Healthy Brazilian individuals |
| title_fullStr |
Whole blood assay to access T Cell-immune responses to Mycobacterium tuberculosis antigens in Healthy Brazilian individuals |
| title_full_unstemmed |
Whole blood assay to access T Cell-immune responses to Mycobacterium tuberculosis antigens in Healthy Brazilian individuals |
| title_sort |
Whole blood assay to access T Cell-immune responses to Mycobacterium tuberculosis antigens in Healthy Brazilian individuals |
| author |
Antas, Paulo R. Z. |
| author_facet |
Antas, Paulo R. Z. Cardoso, Fernando L. L. Oliveira, Eliane B. Gomes, Patrícia K. C. Cunha, Kátia S. Sarno, Euzenir N. Sampaio, Elizabeth P. |
| author_role |
author |
| author2 |
Cardoso, Fernando L. L. Oliveira, Eliane B. Gomes, Patrícia K. C. Cunha, Kátia S. Sarno, Euzenir N. Sampaio, Elizabeth P. |
| author2_role |
author author author author author author |
| dc.contributor.author.fl_str_mv |
Antas, Paulo R. Z. Cardoso, Fernando L. L. Oliveira, Eliane B. Gomes, Patrícia K. C. Cunha, Kátia S. Sarno, Euzenir N. Sampaio, Elizabeth P. |
| dc.subject.other.pt_BR.fl_str_mv |
Tuberculose PPD Infecção latente de Tubereculose Interferon y Respostas de Células T |
| topic |
Tuberculose PPD Infecção latente de Tubereculose Interferon y Respostas de Células T Tuberculosis T cell-responses Whole blood PPD Latent TB infections |
| dc.subject.en.pt_BR.fl_str_mv |
Tuberculosis T cell-responses Whole blood PPD Latent TB infections |
| description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. |
| publishDate |
2004 |
| dc.date.issued.fl_str_mv |
2004 |
| dc.date.accessioned.fl_str_mv |
2020-05-29T13:11:28Z |
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2020-05-29T13:11:28Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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| dc.identifier.citation.fl_str_mv |
ANTAS, Paulo R. Z. et al. Whole Blood Assay to Access T Cell-immune responses to Mycobacterium tuberculosis antigens in Health Brazilian individuals. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 99, n. 1, p. 53-55, Feb. 2004. |
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https://www.arca.fiocruz.br/handle/icict/41445 |
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0074-0276 |
| dc.identifier.doi.pt_BR.fl_str_mv |
10.1590/S0074-02762004000100009 |
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1678-8060 |
| identifier_str_mv |
ANTAS, Paulo R. Z. et al. Whole Blood Assay to Access T Cell-immune responses to Mycobacterium tuberculosis antigens in Health Brazilian individuals. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 99, n. 1, p. 53-55, Feb. 2004. 0074-0276 10.1590/S0074-02762004000100009 1678-8060 |
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