Co-circulation of genotypes IA and IB of hepatitis A virus in Northeast Brazil

Detalhes bibliográficos
Autor(a) principal: Villar, Livia M.
Data de Publicação: 2006
Outros Autores: Morais, L. M., Aloise, R., Melo, M. M. M., Calado, I. A., Lampe, Elisabeth, Gaspar, A .M. C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/39765
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.
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spelling Villar, Livia M.Morais, L. M.Aloise, R.Melo, M. M. M.Calado, I. A.Lampe, ElisabethGaspar, A .M. C.2020-02-06T12:57:15Z2020-02-06T12:57:15Z2006VILLAR, Livia M. et al. Co-circulation of genotypes IA and IB of hepatitis A virus in Northeast Brazil. Brazilian Journal of Medical and Biological Research, v. 39, p. 873-881, 2006.0100-879Xhttps://www.arca.fiocruz.br/handle/icict/3976510.1590/s0100-879x20060007000041414-431XengAssociação Brasileira de Divulgação CientíficaVírus da Hepatite AEpidemiologia molecularSubgenótipo IASubgenótipo IBHepatitis A virusMolecular epidemiologySub-genotype IASub-genotype ICo-circulation of genotypes IA and IB of hepatitis A virus in Northeast Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.Laboratório Central de Saúde Pública do Estado de Pernambuco. Recife, PE, Brasil.Laboratório Central de Saúde Pública do Estado de Pernambuco. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.The Northeast region is the location of most cases of acute hepatitis A virus (HAV) in Brazil. In the present study, the genotypes of HAV strains from Pernambuco State, one of most populous states in the Northeast region, were characterized. Blood samples positive for anti-HAV IgM from 145 individuals (mean age = 29.1 years), collected during 2002 and 2003, were submitted to nested RT-PCR for amplification of the 5'non-translated region (5'NTR) and VP1/2A regions of the HAV genome. The VP1/2A and 5'NTR regions were amplified in 39 and 21% of the samples, respectively. Nucleotide sequencing was carried out in 46% of VP1/2A and in 53% of 5'NTR isolates. The identity in nucleotide sequence of the VP1/2A region ranged from 93.6 to 100.0%. Phylogenetic analysis of the VP1/2A sequences showed that 65% belong to sub-genotype IA and 35% to sub-genotype IB. Co-circulation of both sub-genotypes was observed in the two years studied. Distinct clusters of highly related sequences were observed in both sub-genotypes, suggesting endemic circulation of HAV strains in this area. In the 5'NTR isolates, 92.7-99.2% identity was observed and two isolates presented one deletion at position 413. Phylogenetic analysis showed that genotype IA strains cluster in the tree in the same way as genotype IB strains, but one IIIA isolate from Spain clusters with genotype IB strains. These results do not allow us to state that 5'NTR could be used to genotype HAV sequences. This is the first report of co-circulation of sub-genotypes IA and IB in this region, providing additional information about the molecular epidemiology of HAV strains in Brazil.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/39765/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALLMMOraes_ElizabethLampe_etal_IOC_2006.pdfLMMOraes_ElizabethLampe_etal_IOC_2006.pdfapplication/pdf60932https://www.arca.fiocruz.br/bitstream/icict/39765/2/LMMOraes_ElizabethLampe_etal_IOC_2006.pdf3208206854fa1462762c14866dce799cMD52TEXTLMMOraes_ElizabethLampe_etal_IOC_2006.pdf.txtLMMOraes_ElizabethLampe_etal_IOC_2006.pdf.txtExtracted texttext/plain32675https://www.arca.fiocruz.br/bitstream/icict/39765/3/LMMOraes_ElizabethLampe_etal_IOC_2006.pdf.txt871edfeb37788cf37f658ab9a7a8a9beMD53icict/397652020-02-07 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dc.title.pt_BR.fl_str_mv Co-circulation of genotypes IA and IB of hepatitis A virus in Northeast Brazil
title Co-circulation of genotypes IA and IB of hepatitis A virus in Northeast Brazil
spellingShingle Co-circulation of genotypes IA and IB of hepatitis A virus in Northeast Brazil
Villar, Livia M.
Vírus da Hepatite A
Epidemiologia molecular
Subgenótipo IA
Subgenótipo IB
Hepatitis A virus
Molecular epidemiology
Sub-genotype IA
Sub-genotype I
title_short Co-circulation of genotypes IA and IB of hepatitis A virus in Northeast Brazil
title_full Co-circulation of genotypes IA and IB of hepatitis A virus in Northeast Brazil
title_fullStr Co-circulation of genotypes IA and IB of hepatitis A virus in Northeast Brazil
title_full_unstemmed Co-circulation of genotypes IA and IB of hepatitis A virus in Northeast Brazil
title_sort Co-circulation of genotypes IA and IB of hepatitis A virus in Northeast Brazil
author Villar, Livia M.
author_facet Villar, Livia M.
Morais, L. M.
Aloise, R.
Melo, M. M. M.
Calado, I. A.
Lampe, Elisabeth
Gaspar, A .M. C.
author_role author
author2 Morais, L. M.
Aloise, R.
Melo, M. M. M.
Calado, I. A.
Lampe, Elisabeth
Gaspar, A .M. C.
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Villar, Livia M.
Morais, L. M.
Aloise, R.
Melo, M. M. M.
Calado, I. A.
Lampe, Elisabeth
Gaspar, A .M. C.
dc.subject.other.pt_BR.fl_str_mv Vírus da Hepatite A
Epidemiologia molecular
Subgenótipo IA
Subgenótipo IB
topic Vírus da Hepatite A
Epidemiologia molecular
Subgenótipo IA
Subgenótipo IB
Hepatitis A virus
Molecular epidemiology
Sub-genotype IA
Sub-genotype I
dc.subject.en.pt_BR.fl_str_mv Hepatitis A virus
Molecular epidemiology
Sub-genotype IA
Sub-genotype I
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.
publishDate 2006
dc.date.issued.fl_str_mv 2006
dc.date.accessioned.fl_str_mv 2020-02-06T12:57:15Z
dc.date.available.fl_str_mv 2020-02-06T12:57:15Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv VILLAR, Livia M. et al. Co-circulation of genotypes IA and IB of hepatitis A virus in Northeast Brazil. Brazilian Journal of Medical and Biological Research, v. 39, p. 873-881, 2006.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/39765
dc.identifier.issn.pt_BR.fl_str_mv 0100-879X
dc.identifier.doi.none.fl_str_mv 10.1590/s0100-879x2006000700004
dc.identifier.eissn.none.fl_str_mv 1414-431X
identifier_str_mv VILLAR, Livia M. et al. Co-circulation of genotypes IA and IB of hepatitis A virus in Northeast Brazil. Brazilian Journal of Medical and Biological Research, v. 39, p. 873-881, 2006.
0100-879X
10.1590/s0100-879x2006000700004
1414-431X
url https://www.arca.fiocruz.br/handle/icict/39765
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
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