Colorimetric microwell plate reverse-hybridization assay for Mycobacterium tuberculosis detection
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2011 |
| Outros Autores: | , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/59638 |
Resumo: | Fundação Estadual de Produção e Pesquisa em Saúde. Centro de Desenvolvimento Científico e Tecnológico. Porto Alegre, RS, Brazil. |
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Michelon, Candice TosiRosso, FrancieleSchmid, Karen BarrosSperhacke, Rosa DeaOliveira, Martha MarthaKritski, Afrânio LineuRezende, LeonidesCosta, Elis Regina DallaRibeiro, Andrezza WoloskiVerza, MirelaCafrune, Patrícia IzquierdoSilva, Márcia Susana NunesKuhleis, DanieleZaha, ArnaldoRossetti, Maria Lucia Rosa2023-07-18T06:24:46Z2023-07-18T06:24:46Z2011MICHELON, Candice Tosi et al. Colorimetric microwell plate reverse-hybridization assay for Mycobacterium tuberculosis detection. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 106, n. 2, p. 194-199, Mar. 2011.0074-0276https://www.arca.fiocruz.br/handle/icict/5963810.1590/S0074-027620110002000131678-8060engFiocruz/IOCColorimetryDNA BacterialHumansMycobacterium tuberculosisNucleic Acid HybridizationOligonucleotide ProbesPolymerase Chain ReactionReagent Kits, DiagnosticSensitivity and SpecificitySputumTuberculosis, PulmonaryExtrapulmonary tuberculosisMolecular diagnosisDiagnostic-accuracyCost-effectivenessMolecular diagnosisTuberculosisAcid amplification testsPolymerase-chain-reactionColorimetric microwell plate reverse-hybridization assay for Mycobacterium tuberculosis detectioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Estadual de Produção e Pesquisa em Saúde. Centro de Desenvolvimento Científico e Tecnológico. Porto Alegre, RS, Brazil.Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia. Programa de Pós-Graduação em Biologia Celular e Molecular. Rio Grande, RS, Brazil.Fundação Estadual de Produção e Pesquisa em Saúde. Centro de Desenvolvimento Científico e Tecnológico. Porto Alegre, RS, Brazil.Universidade de Caxias do Sul. Laboratório de Pesquisa em HIV/AIDS. Caxias do Sul, RS, Brazil.Universidade Federal do Rio de Janeiro. Faculdade de Medicina. Rio de Janeiro, RJ, Brazil.Universidade Federal do Rio de Janeiro. Faculdade de Medicina. Rio de Janeiro, RJ, Brazil.Ampligenix Biotech. Belo Horizonte, MG, Brazil.Fundação Estadual de Produção e Pesquisa em Saúde. Centro de Desenvolvimento Científico e Tecnológico. Porto Alegre, RS, Brazil.Fundação Estadual de Produção e Pesquisa em Saúde. Centro de Desenvolvimento Científico e Tecnológico. Porto Alegre, RS, Brazil.Fundação Estadual de Produção e Pesquisa em Saúde. Centro de Desenvolvimento Científico e Tecnológico. Porto Alegre, RS, Brazil.Centro de Biotecnologia, Universidade Federal do Rio Grande Do sul, BrazilCentro de Desenvolvimento Científico e Tecnológico, Fundação Estadual de Produção e Pesquisa em Saúde, Brazil / Universidade Luterana do Brasil, BrazilUniversidade Federal do Rio Grande do Sul. Centro de Biotecnologia. Porto Alegre, RS, Brazil.Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia. Porto Alegre, RS, Brazil.Fundação Estadual de Produção e Pesquisa em Saúde. Centro de Desenvolvimento Científico e Tecnológico. Porto Alegre, RS, Brazil / Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ, Brasil.Direct smear examination using Ziehl-Neelsen staining for pulmonary tuberculosis (PTB) diagnosis is inexpensive and easy to use, but has the major limitation of low sensitivity. Rapid molecular methods are becoming more widely available in centralized laboratories, but they depend on timely reporting of results and strict quality assurance obtainable only from costly commercial kits available in high burden nations. This study describes a pre-commercial colorimetric method, Detect-TB, for detecting Mycobacterium tuberculosis DNA in which an oligonucleotide probe is fixed onto wells of microwell plates and hybridized with biotinylated polymerase chain reaction amplification products derived from clinical samples. The probe is capable of hybridising with the IS6110 insertion element and was used to specifically recognise the M. tuberculosis complex. When combined with an improved silica-based DNA extraction method, the sensitivity of the test was 50 colony-forming units of the M. tuberculosis reference strain H37Rv. The results that were in agreement with reference detection methods were observed in 95.2% (453/476) of samples included in the analysis. Sensitivity and specificity for 301 induced sputum samples and 175 spontaneous sputum samples were 85% and 98%, and 94% and 100%, respectively. This colorimetric method showed similar specificity to that described for commercially available kits and may provide an important contribution for PTB diagnosis.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/59638/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51ORIGINAL10.1590_s0074-02762011000200013.pdfapplication/pdf332837https://www.arca.fiocruz.br/bitstream/icict/59638/2/10.1590_s0074-02762011000200013.pdfabb6b349c9523f537f0046099be60994MD52icict/596382023-07-18 21:03:25.87oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-07-19T00:03:25Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.en_US.fl_str_mv |
Colorimetric microwell plate reverse-hybridization assay for Mycobacterium tuberculosis detection |
| title |
Colorimetric microwell plate reverse-hybridization assay for Mycobacterium tuberculosis detection |
| spellingShingle |
Colorimetric microwell plate reverse-hybridization assay for Mycobacterium tuberculosis detection Michelon, Candice Tosi Colorimetry DNA Bacterial Humans Mycobacterium tuberculosis Nucleic Acid Hybridization Oligonucleotide Probes Polymerase Chain Reaction Reagent Kits, Diagnostic Sensitivity and Specificity Sputum Tuberculosis, Pulmonary Extrapulmonary tuberculosis Molecular diagnosis Diagnostic-accuracy Cost-effectiveness Molecular diagnosis Tuberculosis Acid amplification tests Polymerase-chain-reaction |
| title_short |
Colorimetric microwell plate reverse-hybridization assay for Mycobacterium tuberculosis detection |
| title_full |
Colorimetric microwell plate reverse-hybridization assay for Mycobacterium tuberculosis detection |
| title_fullStr |
Colorimetric microwell plate reverse-hybridization assay for Mycobacterium tuberculosis detection |
| title_full_unstemmed |
Colorimetric microwell plate reverse-hybridization assay for Mycobacterium tuberculosis detection |
| title_sort |
Colorimetric microwell plate reverse-hybridization assay for Mycobacterium tuberculosis detection |
| author |
Michelon, Candice Tosi |
| author_facet |
Michelon, Candice Tosi Rosso, Franciele Schmid, Karen Barros Sperhacke, Rosa Dea Oliveira, Martha Martha Kritski, Afrânio Lineu Rezende, Leonides Costa, Elis Regina Dalla Ribeiro, Andrezza Woloski Verza, Mirela Cafrune, Patrícia Izquierdo Silva, Márcia Susana Nunes Kuhleis, Daniele Zaha, Arnaldo Rossetti, Maria Lucia Rosa |
| author_role |
author |
| author2 |
Rosso, Franciele Schmid, Karen Barros Sperhacke, Rosa Dea Oliveira, Martha Martha Kritski, Afrânio Lineu Rezende, Leonides Costa, Elis Regina Dalla Ribeiro, Andrezza Woloski Verza, Mirela Cafrune, Patrícia Izquierdo Silva, Márcia Susana Nunes Kuhleis, Daniele Zaha, Arnaldo Rossetti, Maria Lucia Rosa |
| author2_role |
author author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Michelon, Candice Tosi Rosso, Franciele Schmid, Karen Barros Sperhacke, Rosa Dea Oliveira, Martha Martha Kritski, Afrânio Lineu Rezende, Leonides Costa, Elis Regina Dalla Ribeiro, Andrezza Woloski Verza, Mirela Cafrune, Patrícia Izquierdo Silva, Márcia Susana Nunes Kuhleis, Daniele Zaha, Arnaldo Rossetti, Maria Lucia Rosa |
| dc.subject.mesh.en_US.fl_str_mv |
Colorimetry DNA Bacterial Humans Mycobacterium tuberculosis Nucleic Acid Hybridization Oligonucleotide Probes Polymerase Chain Reaction Reagent Kits, Diagnostic Sensitivity and Specificity Sputum Tuberculosis, Pulmonary |
| topic |
Colorimetry DNA Bacterial Humans Mycobacterium tuberculosis Nucleic Acid Hybridization Oligonucleotide Probes Polymerase Chain Reaction Reagent Kits, Diagnostic Sensitivity and Specificity Sputum Tuberculosis, Pulmonary Extrapulmonary tuberculosis Molecular diagnosis Diagnostic-accuracy Cost-effectiveness Molecular diagnosis Tuberculosis Acid amplification tests Polymerase-chain-reaction |
| dc.subject.en.en_US.fl_str_mv |
Extrapulmonary tuberculosis Molecular diagnosis Diagnostic-accuracy Cost-effectiveness Molecular diagnosis Tuberculosis Acid amplification tests Polymerase-chain-reaction |
| description |
Fundação Estadual de Produção e Pesquisa em Saúde. Centro de Desenvolvimento Científico e Tecnológico. Porto Alegre, RS, Brazil. |
| publishDate |
2011 |
| dc.date.issued.fl_str_mv |
2011 |
| dc.date.accessioned.fl_str_mv |
2023-07-18T06:24:46Z |
| dc.date.available.fl_str_mv |
2023-07-18T06:24:46Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
| dc.identifier.citation.fl_str_mv |
MICHELON, Candice Tosi et al. Colorimetric microwell plate reverse-hybridization assay for Mycobacterium tuberculosis detection. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 106, n. 2, p. 194-199, Mar. 2011. |
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https://www.arca.fiocruz.br/handle/icict/59638 |
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0074-0276 |
| dc.identifier.doi.none.fl_str_mv |
10.1590/S0074-02762011000200013 |
| dc.identifier.eissn.none.fl_str_mv |
1678-8060 |
| identifier_str_mv |
MICHELON, Candice Tosi et al. Colorimetric microwell plate reverse-hybridization assay for Mycobacterium tuberculosis detection. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 106, n. 2, p. 194-199, Mar. 2011. 0074-0276 10.1590/S0074-02762011000200013 1678-8060 |
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https://www.arca.fiocruz.br/handle/icict/59638 |
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eng |
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Fiocruz/IOC |
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Fiocruz/IOC |
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