Enhancing tuberculosis diagnosis by polymerase chain reactio : an experience at a tertiary hospital

Detalhes bibliográficos
Autor(a) principal: Paris, Fernanda de
Data de Publicação: 2016
Outros Autores: Voigt, Francine, Machado, Alice Beatriz Mombach Pinheiro, Oliveira, Kátia Ruschel Pilger de, Willers, Denise Maria Cunha, Mayora, Dirce Veloso, Paiva, Rodrigo Minuto, Barth, Afonso Luis
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRGS
Texto Completo: http://hdl.handle.net/10183/158418
Resumo: Introduction: Tuberculosis (TB) persists as a severe global public health issue. The aim of the present study was to evaluate the performance of an in-house TB PCR (polymerase chain reaction) in sputum. Methods: DNA from sputum specimens were submitted to a nested-PCR protocol for the IS6110 region detection. PCR results were compared to those of the traditional methods for TB diagnosis, i.e., acid-fast bacilli (AFB) smear microscopy and culture. We analyzed sputum samples obtained from 133 patients. Results: A total of 48 (36%) cultures yielded indeterminate results due to contamination. This high contamination rate may be explained by the fact that samples from fibrocystic patients were included in this study. Additionally, other five samples were positive for nontuberculous mycobacteria (NTM). Therefore, it was possible to compare 80 patients for M. tuberculosis detection. We found 14 positive samples: five presented positive results in the three methods (5/14; 35.7%), two were positive in culture and PCR (2/14; 14.3%), one was positive in AFB and PCR (1/14; 7.1%), five were positive only in PCR (5/14; 35.7%) and 1 was positive only in culture (1/14; 7.1%). Thus, positivity rates for each technique were: 7.5% for AFB (6/80), 10% for culture (8/80) and 16.25% for PCR (13/80). Among the 48 patients who had indeterminate results in sputum culture, two samples were positive in PCR. Conclusion: Considering the limitations of the traditional methods, the use of PCR as a molecular technique could be advantageous for TB diagnosis.
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spelling Paris, Fernanda deVoigt, FrancineMachado, Alice Beatriz Mombach PinheiroOliveira, Kátia Ruschel Pilger deWillers, Denise Maria CunhaMayora, Dirce VelosoPaiva, Rodrigo MinutoBarth, Afonso Luis2017-05-24T02:26:11Z20162357-9730http://hdl.handle.net/10183/158418001012063Introduction: Tuberculosis (TB) persists as a severe global public health issue. The aim of the present study was to evaluate the performance of an in-house TB PCR (polymerase chain reaction) in sputum. Methods: DNA from sputum specimens were submitted to a nested-PCR protocol for the IS6110 region detection. PCR results were compared to those of the traditional methods for TB diagnosis, i.e., acid-fast bacilli (AFB) smear microscopy and culture. We analyzed sputum samples obtained from 133 patients. Results: A total of 48 (36%) cultures yielded indeterminate results due to contamination. This high contamination rate may be explained by the fact that samples from fibrocystic patients were included in this study. Additionally, other five samples were positive for nontuberculous mycobacteria (NTM). Therefore, it was possible to compare 80 patients for M. tuberculosis detection. We found 14 positive samples: five presented positive results in the three methods (5/14; 35.7%), two were positive in culture and PCR (2/14; 14.3%), one was positive in AFB and PCR (1/14; 7.1%), five were positive only in PCR (5/14; 35.7%) and 1 was positive only in culture (1/14; 7.1%). Thus, positivity rates for each technique were: 7.5% for AFB (6/80), 10% for culture (8/80) and 16.25% for PCR (13/80). Among the 48 patients who had indeterminate results in sputum culture, two samples were positive in PCR. Conclusion: Considering the limitations of the traditional methods, the use of PCR as a molecular technique could be advantageous for TB diagnosis.application/pdfengClinical and biomedical research. Porto Alegre. Vol. 36 , n. 1 , (2016), p. 18-22TuberculoseSaúde públicaDiagnósticoMycobacterium sp.Pulmonary tuberculosisNucleic acid amplification testsPolymerase chain reactionEnhancing tuberculosis diagnosis by polymerase chain reactio : an experience at a tertiary hospitalinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL001012063.pdf001012063.pdfTexto completo (inglês)application/pdf726954http://www.lume.ufrgs.br/bitstream/10183/158418/1/001012063.pdfc773c1929367e1ec3007671dd63ecb1bMD51TEXT001012063.pdf.txt001012063.pdf.txtExtracted Texttext/plain18726http://www.lume.ufrgs.br/bitstream/10183/158418/2/001012063.pdf.txtb706e9f4f1738b3bb973999a205894d7MD5210183/1584182023-05-21 03:27:59.106241oai:www.lume.ufrgs.br:10183/158418Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2023-05-21T06:27:59Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.pt_BR.fl_str_mv Enhancing tuberculosis diagnosis by polymerase chain reactio : an experience at a tertiary hospital
title Enhancing tuberculosis diagnosis by polymerase chain reactio : an experience at a tertiary hospital
spellingShingle Enhancing tuberculosis diagnosis by polymerase chain reactio : an experience at a tertiary hospital
Paris, Fernanda de
Tuberculose
Saúde pública
Diagnóstico
Mycobacterium sp.
Pulmonary tuberculosis
Nucleic acid amplification tests
Polymerase chain reaction
title_short Enhancing tuberculosis diagnosis by polymerase chain reactio : an experience at a tertiary hospital
title_full Enhancing tuberculosis diagnosis by polymerase chain reactio : an experience at a tertiary hospital
title_fullStr Enhancing tuberculosis diagnosis by polymerase chain reactio : an experience at a tertiary hospital
title_full_unstemmed Enhancing tuberculosis diagnosis by polymerase chain reactio : an experience at a tertiary hospital
title_sort Enhancing tuberculosis diagnosis by polymerase chain reactio : an experience at a tertiary hospital
author Paris, Fernanda de
author_facet Paris, Fernanda de
Voigt, Francine
Machado, Alice Beatriz Mombach Pinheiro
Oliveira, Kátia Ruschel Pilger de
Willers, Denise Maria Cunha
Mayora, Dirce Veloso
Paiva, Rodrigo Minuto
Barth, Afonso Luis
author_role author
author2 Voigt, Francine
Machado, Alice Beatriz Mombach Pinheiro
Oliveira, Kátia Ruschel Pilger de
Willers, Denise Maria Cunha
Mayora, Dirce Veloso
Paiva, Rodrigo Minuto
Barth, Afonso Luis
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Paris, Fernanda de
Voigt, Francine
Machado, Alice Beatriz Mombach Pinheiro
Oliveira, Kátia Ruschel Pilger de
Willers, Denise Maria Cunha
Mayora, Dirce Veloso
Paiva, Rodrigo Minuto
Barth, Afonso Luis
dc.subject.por.fl_str_mv Tuberculose
Saúde pública
Diagnóstico
topic Tuberculose
Saúde pública
Diagnóstico
Mycobacterium sp.
Pulmonary tuberculosis
Nucleic acid amplification tests
Polymerase chain reaction
dc.subject.eng.fl_str_mv Mycobacterium sp.
Pulmonary tuberculosis
Nucleic acid amplification tests
Polymerase chain reaction
description Introduction: Tuberculosis (TB) persists as a severe global public health issue. The aim of the present study was to evaluate the performance of an in-house TB PCR (polymerase chain reaction) in sputum. Methods: DNA from sputum specimens were submitted to a nested-PCR protocol for the IS6110 region detection. PCR results were compared to those of the traditional methods for TB diagnosis, i.e., acid-fast bacilli (AFB) smear microscopy and culture. We analyzed sputum samples obtained from 133 patients. Results: A total of 48 (36%) cultures yielded indeterminate results due to contamination. This high contamination rate may be explained by the fact that samples from fibrocystic patients were included in this study. Additionally, other five samples were positive for nontuberculous mycobacteria (NTM). Therefore, it was possible to compare 80 patients for M. tuberculosis detection. We found 14 positive samples: five presented positive results in the three methods (5/14; 35.7%), two were positive in culture and PCR (2/14; 14.3%), one was positive in AFB and PCR (1/14; 7.1%), five were positive only in PCR (5/14; 35.7%) and 1 was positive only in culture (1/14; 7.1%). Thus, positivity rates for each technique were: 7.5% for AFB (6/80), 10% for culture (8/80) and 16.25% for PCR (13/80). Among the 48 patients who had indeterminate results in sputum culture, two samples were positive in PCR. Conclusion: Considering the limitations of the traditional methods, the use of PCR as a molecular technique could be advantageous for TB diagnosis.
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dc.relation.ispartof.pt_BR.fl_str_mv Clinical and biomedical research. Porto Alegre. Vol. 36 , n. 1 , (2016), p. 18-22
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