Enhancing tuberculosis diagnosis by polymerase chain reactio : an experience at a tertiary hospital
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/158418 |
Resumo: | Introduction: Tuberculosis (TB) persists as a severe global public health issue. The aim of the present study was to evaluate the performance of an in-house TB PCR (polymerase chain reaction) in sputum. Methods: DNA from sputum specimens were submitted to a nested-PCR protocol for the IS6110 region detection. PCR results were compared to those of the traditional methods for TB diagnosis, i.e., acid-fast bacilli (AFB) smear microscopy and culture. We analyzed sputum samples obtained from 133 patients. Results: A total of 48 (36%) cultures yielded indeterminate results due to contamination. This high contamination rate may be explained by the fact that samples from fibrocystic patients were included in this study. Additionally, other five samples were positive for nontuberculous mycobacteria (NTM). Therefore, it was possible to compare 80 patients for M. tuberculosis detection. We found 14 positive samples: five presented positive results in the three methods (5/14; 35.7%), two were positive in culture and PCR (2/14; 14.3%), one was positive in AFB and PCR (1/14; 7.1%), five were positive only in PCR (5/14; 35.7%) and 1 was positive only in culture (1/14; 7.1%). Thus, positivity rates for each technique were: 7.5% for AFB (6/80), 10% for culture (8/80) and 16.25% for PCR (13/80). Among the 48 patients who had indeterminate results in sputum culture, two samples were positive in PCR. Conclusion: Considering the limitations of the traditional methods, the use of PCR as a molecular technique could be advantageous for TB diagnosis. |
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Paris, Fernanda deVoigt, FrancineMachado, Alice Beatriz Mombach PinheiroOliveira, Kátia Ruschel Pilger deWillers, Denise Maria CunhaMayora, Dirce VelosoPaiva, Rodrigo MinutoBarth, Afonso Luis2017-05-24T02:26:11Z20162357-9730http://hdl.handle.net/10183/158418001012063Introduction: Tuberculosis (TB) persists as a severe global public health issue. The aim of the present study was to evaluate the performance of an in-house TB PCR (polymerase chain reaction) in sputum. Methods: DNA from sputum specimens were submitted to a nested-PCR protocol for the IS6110 region detection. PCR results were compared to those of the traditional methods for TB diagnosis, i.e., acid-fast bacilli (AFB) smear microscopy and culture. We analyzed sputum samples obtained from 133 patients. Results: A total of 48 (36%) cultures yielded indeterminate results due to contamination. This high contamination rate may be explained by the fact that samples from fibrocystic patients were included in this study. Additionally, other five samples were positive for nontuberculous mycobacteria (NTM). Therefore, it was possible to compare 80 patients for M. tuberculosis detection. We found 14 positive samples: five presented positive results in the three methods (5/14; 35.7%), two were positive in culture and PCR (2/14; 14.3%), one was positive in AFB and PCR (1/14; 7.1%), five were positive only in PCR (5/14; 35.7%) and 1 was positive only in culture (1/14; 7.1%). Thus, positivity rates for each technique were: 7.5% for AFB (6/80), 10% for culture (8/80) and 16.25% for PCR (13/80). Among the 48 patients who had indeterminate results in sputum culture, two samples were positive in PCR. Conclusion: Considering the limitations of the traditional methods, the use of PCR as a molecular technique could be advantageous for TB diagnosis.application/pdfengClinical and biomedical research. Porto Alegre. Vol. 36 , n. 1 , (2016), p. 18-22TuberculoseSaúde públicaDiagnósticoMycobacterium sp.Pulmonary tuberculosisNucleic acid amplification testsPolymerase chain reactionEnhancing tuberculosis diagnosis by polymerase chain reactio : an experience at a tertiary hospitalinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL001012063.pdf001012063.pdfTexto completo (inglês)application/pdf726954http://www.lume.ufrgs.br/bitstream/10183/158418/1/001012063.pdfc773c1929367e1ec3007671dd63ecb1bMD51TEXT001012063.pdf.txt001012063.pdf.txtExtracted Texttext/plain18726http://www.lume.ufrgs.br/bitstream/10183/158418/2/001012063.pdf.txtb706e9f4f1738b3bb973999a205894d7MD5210183/1584182023-05-21 03:27:59.106241oai:www.lume.ufrgs.br:10183/158418Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2023-05-21T06:27:59Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Enhancing tuberculosis diagnosis by polymerase chain reactio : an experience at a tertiary hospital |
title |
Enhancing tuberculosis diagnosis by polymerase chain reactio : an experience at a tertiary hospital |
spellingShingle |
Enhancing tuberculosis diagnosis by polymerase chain reactio : an experience at a tertiary hospital Paris, Fernanda de Tuberculose Saúde pública Diagnóstico Mycobacterium sp. Pulmonary tuberculosis Nucleic acid amplification tests Polymerase chain reaction |
title_short |
Enhancing tuberculosis diagnosis by polymerase chain reactio : an experience at a tertiary hospital |
title_full |
Enhancing tuberculosis diagnosis by polymerase chain reactio : an experience at a tertiary hospital |
title_fullStr |
Enhancing tuberculosis diagnosis by polymerase chain reactio : an experience at a tertiary hospital |
title_full_unstemmed |
Enhancing tuberculosis diagnosis by polymerase chain reactio : an experience at a tertiary hospital |
title_sort |
Enhancing tuberculosis diagnosis by polymerase chain reactio : an experience at a tertiary hospital |
author |
Paris, Fernanda de |
author_facet |
Paris, Fernanda de Voigt, Francine Machado, Alice Beatriz Mombach Pinheiro Oliveira, Kátia Ruschel Pilger de Willers, Denise Maria Cunha Mayora, Dirce Veloso Paiva, Rodrigo Minuto Barth, Afonso Luis |
author_role |
author |
author2 |
Voigt, Francine Machado, Alice Beatriz Mombach Pinheiro Oliveira, Kátia Ruschel Pilger de Willers, Denise Maria Cunha Mayora, Dirce Veloso Paiva, Rodrigo Minuto Barth, Afonso Luis |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Paris, Fernanda de Voigt, Francine Machado, Alice Beatriz Mombach Pinheiro Oliveira, Kátia Ruschel Pilger de Willers, Denise Maria Cunha Mayora, Dirce Veloso Paiva, Rodrigo Minuto Barth, Afonso Luis |
dc.subject.por.fl_str_mv |
Tuberculose Saúde pública Diagnóstico |
topic |
Tuberculose Saúde pública Diagnóstico Mycobacterium sp. Pulmonary tuberculosis Nucleic acid amplification tests Polymerase chain reaction |
dc.subject.eng.fl_str_mv |
Mycobacterium sp. Pulmonary tuberculosis Nucleic acid amplification tests Polymerase chain reaction |
description |
Introduction: Tuberculosis (TB) persists as a severe global public health issue. The aim of the present study was to evaluate the performance of an in-house TB PCR (polymerase chain reaction) in sputum. Methods: DNA from sputum specimens were submitted to a nested-PCR protocol for the IS6110 region detection. PCR results were compared to those of the traditional methods for TB diagnosis, i.e., acid-fast bacilli (AFB) smear microscopy and culture. We analyzed sputum samples obtained from 133 patients. Results: A total of 48 (36%) cultures yielded indeterminate results due to contamination. This high contamination rate may be explained by the fact that samples from fibrocystic patients were included in this study. Additionally, other five samples were positive for nontuberculous mycobacteria (NTM). Therefore, it was possible to compare 80 patients for M. tuberculosis detection. We found 14 positive samples: five presented positive results in the three methods (5/14; 35.7%), two were positive in culture and PCR (2/14; 14.3%), one was positive in AFB and PCR (1/14; 7.1%), five were positive only in PCR (5/14; 35.7%) and 1 was positive only in culture (1/14; 7.1%). Thus, positivity rates for each technique were: 7.5% for AFB (6/80), 10% for culture (8/80) and 16.25% for PCR (13/80). Among the 48 patients who had indeterminate results in sputum culture, two samples were positive in PCR. Conclusion: Considering the limitations of the traditional methods, the use of PCR as a molecular technique could be advantageous for TB diagnosis. |
publishDate |
2016 |
dc.date.issued.fl_str_mv |
2016 |
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2017-05-24T02:26:11Z |
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http://hdl.handle.net/10183/158418 |
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2357-9730 |
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001012063 |
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Clinical and biomedical research. Porto Alegre. Vol. 36 , n. 1 , (2016), p. 18-22 |
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