Molecular identification of Coccidioides spp. in soil samples from Brazil

Detalhes bibliográficos
Autor(a) principal: Macêdo, Regina C. L. de
Data de Publicação: 2011
Outros Autores: Rosado, Alexandre S., Mota, Fabio F. da, Cavalcante, Maria A. S., Eulálio, Kelsen D., D. Filho, Antônio, Martins, Liline M. S., Lazéra, Márcia S., Wanke, Bodo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/15993
Resumo: Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Laboratório de Ecologia Microbiana Molecular. Rio de Janeiro, RJ, Brasil.
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spelling Macêdo, Regina C. L. deRosado, Alexandre S.Mota, Fabio F. daCavalcante, Maria A. S.Eulálio, Kelsen D.D. Filho, AntônioMartins, Liline M. S.Lazéra, Márcia S.Wanke, Bodo2016-09-29T11:37:48Z2016-09-29T11:37:48Z2011MACÊDO, Regina C. L. de et al. Molecular identification of Coccidioides spp. in soil samples from Brazil. BMC Microbiology, v. 11, n. 108, p. 1-9, 2011.1471-2180https://www.arca.fiocruz.br/handle/icict/1599310.1186/1471-2180-11-108engBioMed CentralRegião NordesteSemi áridoBrasilReação em Cadeia da PolimeraseCoccidioidomicoseCoccidioidesCoccidioidomycosisNortheast of BrazilCoccidioides sppC. posadasiiSemi-aridSemi-nested PCRMolecular identification of Coccidioides spp. in soil samples from Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Laboratório de Ecologia Microbiana Molecular. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Laboratório de Ecologia Microbiana Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Computacional e Sistemas. Rio de Janeiro, RJ, Brasil.Universidade Estadual do Piauí. Teresina, PI, Brasil / Universidade Federal do Piauí. Teresina, PI, Brasil.Universidade Federal do Piauí. Teresina, PI, Brasil.Universidade Federal do Piauí. Teresina, PI, Brasil.Universidade Estadual do Piauí. Teresina, PI, Brasil / Universidade Federal do Piauí. Teresina, PI, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Background: Since 1991 several outbreaks of acute coccidioidomycosis (CM) were diagnosed in the semi-arid Northeast of Brazil, mainly related to disturbance of armadillo burrows caused by hunters while digging them for the capture of these animals. This activity causes dust contaminated with arthroconidia of Coccidioides posadasii, which, once inhaled, cause the mycosis. We report on the identification of C. posadasii in soil samples related to outbreaks of CM. Results: Twenty four soil samples had their DNA extracted and subsequently submitted to a semi-nested PCR technique using specific primers. While only 6 (25%) soil samples were positive for C. posadasii by mice inoculation, all (100%) were positive by the molecular tool. Conclusion: This methodology represents a simple, sensitive and specific molecular technique to determine the environmental distribution of Coccidioides spp. in endemic areas, but cannot distinguish the species. Moreover, it may be useful to identify culture isolates. Key-words: 1. Coccidioidomycosis. 2. Coccidioides spp. 3. C. posadasii. 4. Semi-arid. 5. Semi-nested PCR.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/15993/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALfabio_mota_etal_IOC_2011.pdffabio_mota_etal_IOC_2011.pdfapplication/pdf635392https://www.arca.fiocruz.br/bitstream/icict/15993/2/fabio_mota_etal_IOC_2011.pdf5d3f0cdb216f4e5777f810a5b4baf410MD52TEXTfabio_mota_etal_IOC_2011.pdf.txtfabio_mota_etal_IOC_2011.pdf.txtExtracted texttext/plain42301https://www.arca.fiocruz.br/bitstream/icict/15993/3/fabio_mota_etal_IOC_2011.pdf.txtd6633fe2b562e12c0791f44d01a172d5MD53icict/159932023-10-25 20:50:08.352oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-10-25T23:50:08Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Molecular identification of Coccidioides spp. in soil samples from Brazil
title Molecular identification of Coccidioides spp. in soil samples from Brazil
spellingShingle Molecular identification of Coccidioides spp. in soil samples from Brazil
Macêdo, Regina C. L. de
Região Nordeste
Semi árido
Brasil
Reação em Cadeia da Polimerase
Coccidioidomicose
Coccidioides
Coccidioidomycosis
Northeast of Brazil
Coccidioides spp
C. posadasii
Semi-arid
Semi-nested PCR
title_short Molecular identification of Coccidioides spp. in soil samples from Brazil
title_full Molecular identification of Coccidioides spp. in soil samples from Brazil
title_fullStr Molecular identification of Coccidioides spp. in soil samples from Brazil
title_full_unstemmed Molecular identification of Coccidioides spp. in soil samples from Brazil
title_sort Molecular identification of Coccidioides spp. in soil samples from Brazil
author Macêdo, Regina C. L. de
author_facet Macêdo, Regina C. L. de
Rosado, Alexandre S.
Mota, Fabio F. da
Cavalcante, Maria A. S.
Eulálio, Kelsen D.
D. Filho, Antônio
Martins, Liline M. S.
Lazéra, Márcia S.
Wanke, Bodo
author_role author
author2 Rosado, Alexandre S.
Mota, Fabio F. da
Cavalcante, Maria A. S.
Eulálio, Kelsen D.
D. Filho, Antônio
Martins, Liline M. S.
Lazéra, Márcia S.
Wanke, Bodo
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Macêdo, Regina C. L. de
Rosado, Alexandre S.
Mota, Fabio F. da
Cavalcante, Maria A. S.
Eulálio, Kelsen D.
D. Filho, Antônio
Martins, Liline M. S.
Lazéra, Márcia S.
Wanke, Bodo
dc.subject.other.pt_BR.fl_str_mv Região Nordeste
Semi árido
Brasil
Reação em Cadeia da Polimerase
Coccidioidomicose
Coccidioides
topic Região Nordeste
Semi árido
Brasil
Reação em Cadeia da Polimerase
Coccidioidomicose
Coccidioides
Coccidioidomycosis
Northeast of Brazil
Coccidioides spp
C. posadasii
Semi-arid
Semi-nested PCR
dc.subject.en.pt_BR.fl_str_mv Coccidioidomycosis
Northeast of Brazil
Coccidioides spp
C. posadasii
Semi-arid
Semi-nested PCR
description Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Laboratório de Ecologia Microbiana Molecular. Rio de Janeiro, RJ, Brasil.
publishDate 2011
dc.date.issued.fl_str_mv 2011
dc.date.accessioned.fl_str_mv 2016-09-29T11:37:48Z
dc.date.available.fl_str_mv 2016-09-29T11:37:48Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MACÊDO, Regina C. L. de et al. Molecular identification of Coccidioides spp. in soil samples from Brazil. BMC Microbiology, v. 11, n. 108, p. 1-9, 2011.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/15993
dc.identifier.issn.pt_BR.fl_str_mv 1471-2180
dc.identifier.doi.none.fl_str_mv 10.1186/1471-2180-11-108
identifier_str_mv MACÊDO, Regina C. L. de et al. Molecular identification of Coccidioides spp. in soil samples from Brazil. BMC Microbiology, v. 11, n. 108, p. 1-9, 2011.
1471-2180
10.1186/1471-2180-11-108
url https://www.arca.fiocruz.br/handle/icict/15993
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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https://www.arca.fiocruz.br/bitstream/icict/15993/2/fabio_mota_etal_IOC_2011.pdf
https://www.arca.fiocruz.br/bitstream/icict/15993/3/fabio_mota_etal_IOC_2011.pdf.txt
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