Histological assessment of granulomas in natural and experimental Schistosoma mansoni infections using whole slide imaging
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/24840 |
Resumo: | Universidade Federal de Juiz de Fora. Departamento de Biologia. Laboratório de Biologia Celular. Juiz de Fora, MG, Brasil. |
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Amaral, Kátia B.Silva, Thiago P.Dias, Felipe F.Malta, Kássia K.Rosa, Florence M.Costa Neto, Sócrates F.Gentile, RosanaMelo, Rossana C. N.2018-02-15T15:47:33Z2018-02-15T15:47:33Z2017AMARAL, Kátia B. et al. Histological assessment of granulomas in natural and experimental Schistosoma mansoni infections using whole slide imaging. PLoS One, v.12, n.9, e0184696, 20p, Sept. 2017.1932-6203https://www.arca.fiocruz.br/handle/icict/2484010.1371/journal.pone.0184696engPublic Library of ScienceAvaliação histológicaSchistosoma mansoniGranulomaInfecçãoSchistosoma mansonigranulomasinfectionsHistological assessmentHistological assessment of granulomas in natural and experimental Schistosoma mansoni infections using whole slide imaginginfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Juiz de Fora. Departamento de Biologia. Laboratório de Biologia Celular. Juiz de Fora, MG, Brasil.Universidade Federal de Juiz de Fora. Departamento de Biologia. Laboratório de Biologia Celular. Juiz de Fora, MG, Brasil.Universidade Federal de Juiz de Fora. Departamento de Biologia. Laboratório de Biologia Celular. Juiz de Fora, MG, Brasil.Universidade Federal de Juiz de Fora. Departamento de Biologia. Laboratório de Biologia Celular. Juiz de Fora, MG, Brasil.Universidade Federal de Juiz de Fora. Departamento de Parasitologia, Microbiologia e Imunologia. Laboratório de Parasitologia. Juiz de Fora, MG, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia e Parasitologia de Mamíferos Silvestres Reservatórios. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia e Parasitologia de Mamíferos Silvestres Reservatórios. Rio de Janeiro, RJ. Brasil.Universidade Federal de Juiz de Fora. Departamento de Biologia. Laboratório de Biologia Celular. Juiz de Fora, MG, Brasil.The pathology of schistosomiasis mansoni, a neglected tropical disease of great clinical and socioeconomic importance, results from the parasite eggs that become trapped in host tissues, particularly in the liver and intestines. Continuous antigenic stimulation from these eggs leads to recruitment of inflammatory cells to the sites of infection with formation of periovular granulomas. These complex structures have variable size and composition and are the most striking histopathological feature of schistosomiasis mansoni. However, evaluation of granulomas by conventional microscopy methods is time-consuming and limited, especially in large-scale studies. Here, we used high resolution Whole Slide Imaging (WSI), which allows fast scanning of entire histological slides, and multiple morphometric evaluations, to assess the granulomatous response elicited in target organs (liver, small and large intestines) of two models of schistosomiasis mansoni. One of the advantages of WSI, also termed virtual microscopy, is that it generates images that simultaneously offer high resolution and a wide field of observation. By using a model of natural (Nectomys squamipes, a wild reservoir captured from endemic areas in Brazil) and experimental (Swiss mouse) infection with Schistosoma mansoni, we provided the first detailed WSI characterization of granulomas and other pathological aspects. WSI and quantitative analyses enabled a fast and reliable assessment of the number, evolutional types, frequency and areas of granulomas and inflammatory infiltrates and revealed that target organs are differentially impacted by inflammatory responses in the natural and experimental infections. Remarkably, high-resolution analysis of individual eosinophils, key cells elicited by this helminthic infection, showed a great difference in eosinophil numbers between the two infections. Moreover, features such as the intestinal egg path and confluent granulomas were uncovered. Thus, WSI may be a suitable tool for detailed and precise histological analysis of granulomas and other pathological aspects for clinical and research studies of schistosomiasis.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/24840/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALrosana_gentile_etal_IOC_2017.pdfrosana_gentile_etal_IOC_2017.pdfapplication/pdf43310021https://www.arca.fiocruz.br/bitstream/icict/24840/2/rosana_gentile_etal_IOC_2017.pdfa5fcca2726ed0ccb857ed0d8624f904bMD52TEXTrosana_gentile_etal_IOC_2017.pdf.txtrosana_gentile_etal_IOC_2017.pdf.txtExtracted texttext/plain54730https://www.arca.fiocruz.br/bitstream/icict/24840/3/rosana_gentile_etal_IOC_2017.pdf.txt17ce3852a69db182dfc28b52f66bfa3eMD53icict/248402018-08-15 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dc.title.pt_BR.fl_str_mv |
Histological assessment of granulomas in natural and experimental Schistosoma mansoni infections using whole slide imaging |
title |
Histological assessment of granulomas in natural and experimental Schistosoma mansoni infections using whole slide imaging |
spellingShingle |
Histological assessment of granulomas in natural and experimental Schistosoma mansoni infections using whole slide imaging Amaral, Kátia B. Avaliação histológica Schistosoma mansoni Granuloma Infecção Schistosoma mansoni granulomas infections Histological assessment |
title_short |
Histological assessment of granulomas in natural and experimental Schistosoma mansoni infections using whole slide imaging |
title_full |
Histological assessment of granulomas in natural and experimental Schistosoma mansoni infections using whole slide imaging |
title_fullStr |
Histological assessment of granulomas in natural and experimental Schistosoma mansoni infections using whole slide imaging |
title_full_unstemmed |
Histological assessment of granulomas in natural and experimental Schistosoma mansoni infections using whole slide imaging |
title_sort |
Histological assessment of granulomas in natural and experimental Schistosoma mansoni infections using whole slide imaging |
author |
Amaral, Kátia B. |
author_facet |
Amaral, Kátia B. Silva, Thiago P. Dias, Felipe F. Malta, Kássia K. Rosa, Florence M. Costa Neto, Sócrates F. Gentile, Rosana Melo, Rossana C. N. |
author_role |
author |
author2 |
Silva, Thiago P. Dias, Felipe F. Malta, Kássia K. Rosa, Florence M. Costa Neto, Sócrates F. Gentile, Rosana Melo, Rossana C. N. |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Amaral, Kátia B. Silva, Thiago P. Dias, Felipe F. Malta, Kássia K. Rosa, Florence M. Costa Neto, Sócrates F. Gentile, Rosana Melo, Rossana C. N. |
dc.subject.other.pt_BR.fl_str_mv |
Avaliação histológica Schistosoma mansoni Granuloma Infecção |
topic |
Avaliação histológica Schistosoma mansoni Granuloma Infecção Schistosoma mansoni granulomas infections Histological assessment |
dc.subject.en.pt_BR.fl_str_mv |
Schistosoma mansoni granulomas infections Histological assessment |
description |
Universidade Federal de Juiz de Fora. Departamento de Biologia. Laboratório de Biologia Celular. Juiz de Fora, MG, Brasil. |
publishDate |
2017 |
dc.date.issued.fl_str_mv |
2017 |
dc.date.accessioned.fl_str_mv |
2018-02-15T15:47:33Z |
dc.date.available.fl_str_mv |
2018-02-15T15:47:33Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
AMARAL, Kátia B. et al. Histological assessment of granulomas in natural and experimental Schistosoma mansoni infections using whole slide imaging. PLoS One, v.12, n.9, e0184696, 20p, Sept. 2017. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/24840 |
dc.identifier.issn.pt_BR.fl_str_mv |
1932-6203 |
dc.identifier.doi.none.fl_str_mv |
10.1371/journal.pone.0184696 |
identifier_str_mv |
AMARAL, Kátia B. et al. Histological assessment of granulomas in natural and experimental Schistosoma mansoni infections using whole slide imaging. PLoS One, v.12, n.9, e0184696, 20p, Sept. 2017. 1932-6203 10.1371/journal.pone.0184696 |
url |
https://www.arca.fiocruz.br/handle/icict/24840 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
dc.publisher.none.fl_str_mv |
Public Library of Science |
publisher.none.fl_str_mv |
Public Library of Science |
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reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
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