An assay for the analysis of lymphocyte migration across cerebral endothelium in vitro

Detalhes bibliográficos
Autor(a) principal: Pryce, Gareth
Data de Publicação: 1993
Outros Autores: dosSantos, Washington Luis Conrado, Male, David
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/19935
Resumo: dos Santos, Washington Luis Carlos “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”.
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spelling Pryce, GarethdosSantos, Washington Luis ConradoMale, David2017-07-10T17:41:34Z2017-07-10T17:41:34Z1993PRYCE, G.; dos SANTOS, W. L. C.; MALE, D. An assay for the analysis of lymphocyte migration across cerebral endothelium in vitro. Journal of Immunological Methods, v. 167, p. 55-63, 1993.0022-1759https://www.arca.fiocruz.br/handle/icict/19935dos Santos, Washington Luis Carlos “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”.Medical Research Council of Great BritainInstitute of Psychiatry. Department of Neuropathology. London, UKInstitute of Psychiatry. Department of Neuropathology. London, UKInstitute of Psychiatry. Department of Neuropathology. London, UKWe describe a recently developed assay for the analysis of leukocyte migration across cerebral endothelium in vitro. The endothelium is grown as monolayers on Goretex or Cyclopore membranes coated with extracellular matrix proteins and supported on inserts. This system permits the recovery and phenotyping of cells which migrate down through the endothelium. Using labelled lymphocytes we were able to differentiate four populations of cells, with differing degrees of mobility in the migration assay. We have compared the results from this system with those from conventional adhesion assays. Binding of cells to the endothelium is rapid, but is confined to a particular subpopulation of the applied lymphocytes. We have followed cell migration over 24 h in the system using normal and cytokine-activated endothelium and have found that whereas adhesion depends both on the state of lymphocyte activation and on the condition of the endothelium, the level of migration of stimulated lymphocytes is largely independent of endothelial activation. Moreover, whereas CD8 ÷ cells bind well to the endothelium, it is the CD4 ÷ cells which migrate most effectively. Comparison of brain and epididymal fat endothelium showed similar migration levels over 2 h, but migration was greater across epididymal fat endothelium at 24 h.engElsevierEndotélioLinfócitosMigraçãoAdesãoEndotheliumLymphocyteMigrationAdhesionAn assay for the analysis of lymphocyte migration across cerebral endothelium in vitroinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/19935/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALPryce G An assay for the analysis....pdfPryce G An assay for the analysis....pdfapplication/pdf1397779https://www.arca.fiocruz.br/bitstream/icict/19935/2/Pryce%20G%20An%20assay%20for%20the%20analysis....pdfc0baf4271abe507200451dff8297e1dbMD52TEXTPryce G An assay for the analysis....pdf.txtPryce 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dc.title.pt_BR.fl_str_mv An assay for the analysis of lymphocyte migration across cerebral endothelium in vitro
title An assay for the analysis of lymphocyte migration across cerebral endothelium in vitro
spellingShingle An assay for the analysis of lymphocyte migration across cerebral endothelium in vitro
Pryce, Gareth
Endotélio
Linfócitos
Migração
Adesão
Endothelium
Lymphocyte
Migration
Adhesion
title_short An assay for the analysis of lymphocyte migration across cerebral endothelium in vitro
title_full An assay for the analysis of lymphocyte migration across cerebral endothelium in vitro
title_fullStr An assay for the analysis of lymphocyte migration across cerebral endothelium in vitro
title_full_unstemmed An assay for the analysis of lymphocyte migration across cerebral endothelium in vitro
title_sort An assay for the analysis of lymphocyte migration across cerebral endothelium in vitro
author Pryce, Gareth
author_facet Pryce, Gareth
dosSantos, Washington Luis Conrado
Male, David
author_role author
author2 dosSantos, Washington Luis Conrado
Male, David
author2_role author
author
dc.contributor.author.fl_str_mv Pryce, Gareth
dosSantos, Washington Luis Conrado
Male, David
dc.subject.other.pt_BR.fl_str_mv Endotélio
Linfócitos
Migração
Adesão
topic Endotélio
Linfócitos
Migração
Adesão
Endothelium
Lymphocyte
Migration
Adhesion
dc.subject.en.pt_BR.fl_str_mv Endothelium
Lymphocyte
Migration
Adhesion
description dos Santos, Washington Luis Carlos “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”.
publishDate 1993
dc.date.issued.fl_str_mv 1993
dc.date.accessioned.fl_str_mv 2017-07-10T17:41:34Z
dc.date.available.fl_str_mv 2017-07-10T17:41:34Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.citation.fl_str_mv PRYCE, G.; dos SANTOS, W. L. C.; MALE, D. An assay for the analysis of lymphocyte migration across cerebral endothelium in vitro. Journal of Immunological Methods, v. 167, p. 55-63, 1993.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/19935
dc.identifier.issn.pt_BR.fl_str_mv 0022-1759
identifier_str_mv PRYCE, G.; dos SANTOS, W. L. C.; MALE, D. An assay for the analysis of lymphocyte migration across cerebral endothelium in vitro. Journal of Immunological Methods, v. 167, p. 55-63, 1993.
0022-1759
url https://www.arca.fiocruz.br/handle/icict/19935
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
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