Twenty Years of DENV-2 Activity in Brazil: Molecular Characterization and Phylogeny of Strains Isolated from 1990 to 2010
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2013 |
| Outros Autores: | , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/12535 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil. |
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Faria, Nieli Rodrigues da CostaNogueira, Rita Maria RibeiroFilippis, Ana Maria Bispo deSimões, Jaqueline Bastos SantosNogueira, Fernanda de BruyckerLima, Monique da Rocha QueirozSantos, Flávia Barreto dos2016-01-14T15:38:15Z2016-01-14T15:38:15Z2013FARIA, Nieli Rodrigues da Costa; et al. Twenty Years of DENV-2 Activity in Brazil: Molecular Characterization and Phylogeny of Strains Isolated from 1990 to 2010. Plos One, 7, n.3, e2095, 8p, 2013.1932-6203https://www.arca.fiocruz.br/handle/icict/1253510.1371/journal.pntd.00020951932-6203engPlos OneTwenty Years of DENV-2 Activity in Brazil: Molecular Characterization and Phylogeny of Strains Isolated from 1990 to 2010info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil.In Brazil, dengue has been a major public health problem since its introduction in the 1980s. Phylogenetic studies constitute a valuable tool to monitor the introduction and spread of viruses as well as to predict the potential epidemiological consequences of such events. Aiming to perform the molecular characterization and phylogenetic analysis of DENV-2 during twenty years of viral activity in the country, viral strains isolated from patients presenting different disease manifestations (n = 34), representing six states of the country, from 1990 to 2010, were sequenced. Partial genome sequencing (genes C/prM/M/E) was performed in 25 DENV-2 strains and full-length genome sequencing (coding region) was performed in 9 strains. The percentage of similarity among the DENV-2 strains in this study and reference strains available in Genbank identified two groups epidemiologically distinct: one represented by strains isolated from 1990 to 2003 and one from strains isolated from 2007 to 2010. No consistent differences were observed on the E gene from strains isolated from cases with different clinical manifestations analyzed, suggesting that if the disease severity has a genetic origin, it is not only due to the differences observed on the E gene. The results obtained by the DENV-2 full-length genome sequencing did not point out consistent differences related to a more severe disease either. The analysis based on the partial and/or complete genome sequencing has characterized the Brazilian DENV-2 strains as belonging to the Southeast Asian genotype, however a distinction of two Lineages within this genotype has been identified. It was established that strains circulating prior DENV-2 emergence (1990–2003) belong to Southeast Asian genotype, Lineage I and strains isolated after DENV-2 emergence in 2007 belong to Southeast Asian genotype, Lineage II. Furthermore, all DENV-2 strains analyzed presented an asparagine (N) in E390, previously identified as a probable genetic marker of virulence observed in DHF strains from Asian origin. The percentage of identity of the latter with the Dominican Republic strain isolated in 2001 combined to the percentage of divergence with the strains first introduced in the country in the 1990s suggests that those viruses did not evolve locally but were due to a new viral Lineage introduction in the country from the Caribbean.DENV-2DengueBrazilDengueFilogeniaHistóriainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/12535/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALniele_faria_etal_IOC_2013.pdfniele_faria_etal_IOC_2013.pdfapplication/pdf479333https://www.arca.fiocruz.br/bitstream/icict/12535/2/niele_faria_etal_IOC_2013.pdf04c1836951c0f11fa79d958314bebca3MD52TEXTniele_faria_etal_IOC_2013.pdf.txtniele_faria_etal_IOC_2013.pdf.txtExtracted 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| dc.title.pt_BR.fl_str_mv |
Twenty Years of DENV-2 Activity in Brazil: Molecular Characterization and Phylogeny of Strains Isolated from 1990 to 2010 |
| title |
Twenty Years of DENV-2 Activity in Brazil: Molecular Characterization and Phylogeny of Strains Isolated from 1990 to 2010 |
| spellingShingle |
Twenty Years of DENV-2 Activity in Brazil: Molecular Characterization and Phylogeny of Strains Isolated from 1990 to 2010 Faria, Nieli Rodrigues da Costa DENV-2 Dengue Brazil Dengue Filogenia História |
| title_short |
Twenty Years of DENV-2 Activity in Brazil: Molecular Characterization and Phylogeny of Strains Isolated from 1990 to 2010 |
| title_full |
Twenty Years of DENV-2 Activity in Brazil: Molecular Characterization and Phylogeny of Strains Isolated from 1990 to 2010 |
| title_fullStr |
Twenty Years of DENV-2 Activity in Brazil: Molecular Characterization and Phylogeny of Strains Isolated from 1990 to 2010 |
| title_full_unstemmed |
Twenty Years of DENV-2 Activity in Brazil: Molecular Characterization and Phylogeny of Strains Isolated from 1990 to 2010 |
| title_sort |
Twenty Years of DENV-2 Activity in Brazil: Molecular Characterization and Phylogeny of Strains Isolated from 1990 to 2010 |
| author |
Faria, Nieli Rodrigues da Costa |
| author_facet |
Faria, Nieli Rodrigues da Costa Nogueira, Rita Maria Ribeiro Filippis, Ana Maria Bispo de Simões, Jaqueline Bastos Santos Nogueira, Fernanda de Bruycker Lima, Monique da Rocha Queiroz Santos, Flávia Barreto dos |
| author_role |
author |
| author2 |
Nogueira, Rita Maria Ribeiro Filippis, Ana Maria Bispo de Simões, Jaqueline Bastos Santos Nogueira, Fernanda de Bruycker Lima, Monique da Rocha Queiroz Santos, Flávia Barreto dos |
| author2_role |
author author author author author author |
| dc.contributor.author.fl_str_mv |
Faria, Nieli Rodrigues da Costa Nogueira, Rita Maria Ribeiro Filippis, Ana Maria Bispo de Simões, Jaqueline Bastos Santos Nogueira, Fernanda de Bruycker Lima, Monique da Rocha Queiroz Santos, Flávia Barreto dos |
| dc.subject.en.pt_BR.fl_str_mv |
DENV-2 Dengue Brazil |
| topic |
DENV-2 Dengue Brazil Dengue Filogenia História |
| dc.subject.decs.pt_BR.fl_str_mv |
Dengue Filogenia História |
| description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil. |
| publishDate |
2013 |
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2013 |
| dc.date.accessioned.fl_str_mv |
2016-01-14T15:38:15Z |
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2016-01-14T15:38:15Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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FARIA, Nieli Rodrigues da Costa; et al. Twenty Years of DENV-2 Activity in Brazil: Molecular Characterization and Phylogeny of Strains Isolated from 1990 to 2010. Plos One, 7, n.3, e2095, 8p, 2013. |
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https://www.arca.fiocruz.br/handle/icict/12535 |
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1932-6203 |
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10.1371/journal.pntd.0002095 |
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1932-6203 |
| identifier_str_mv |
FARIA, Nieli Rodrigues da Costa; et al. Twenty Years of DENV-2 Activity in Brazil: Molecular Characterization and Phylogeny of Strains Isolated from 1990 to 2010. Plos One, 7, n.3, e2095, 8p, 2013. 1932-6203 10.1371/journal.pntd.0002095 |
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Plos One |
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