Evaluation of a new set of recombinant antigens for the serological diagnosis of human and canine visceral leishmaniasis
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2017 |
| Outros Autores: | , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/22955 |
Resumo: | Brazilian funding agency CNPq and the Brazilian Ministry of Health (Grant MCTI/CNPq/MS-SCTIENo.: 404259/2012). |
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Magalhães, Franklin BarbalhoCastro Neto, Artur Leonel deNascimento, Marilia BSantos, Wagner José Tenório dosMedeiros, Zulma Maria deLima Neto, Adelino SoaresCosta, Dorcas LamounierCosta, Carlos H NdosSantos, Washington Luis ConradoPontes de Carvalho, Lain CarlosOliveira, Geraldo Gileno de SáMelo Neto, Osvaldo Pompilio de2017-10-25T13:04:19Z2017-10-25T13:04:19Z2017MAGALHÃES, F. B. et al. Evaluation of a new set of recombinant antigens for the serological diagnosis of human and canine visceral leishmaniasis. Plos One, sep. 2017.1932-6203https://www.arca.fiocruz.br/handle/icict/2295510.1371/journal.pone.0184867Brazilian funding agency CNPq and the Brazilian Ministry of Health (Grant MCTI/CNPq/MS-SCTIENo.: 404259/2012).Associação Caruaruense de Ensino Superior e Técnico. Caruaru, PE, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Aggeu Magalhães. Recife, PE, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Aggeu Magalhães. Recife, PE, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Aggeu Magalhães. Recife, PE, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Aggeu Magalhães. Recife, PE, BrasilInstituto de Doenças Tropicais Natan Portella. Teresina, PI, BrasilInstituto de Doenças Tropicais Natan Portella. Teresina, PI, BrasilInstituto de Doenças Tropicais Natan Portella. Teresina, PI, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Aggeu Magalhães. Recife, PE, BrasilCurrent strategies for the control of zoonotic visceral leishmaniasis (VL) rely on its efficient diagnosis in both human and canine hosts. The most promising and cost effective approach is based on serologic assays with recombinant proteins. However, no single antigen has been found so far which can be effectively used to detect the disease in both dogs and humans. In previous works, we identified Leishmania infantum antigens with potential for the serodiagnosis of VL. Here, we aimed to expand the panel of the available antigens for VL diagnosis through another screening of a genomic expression library. Seven different protein-coding gene fragments were identified, five of which encoding proteins which have not been previously studied in Leishmania and rich in repetitive motifs. Poly-histidine tagged polypeptides were generated from six genes and evaluated for their potential for diagnosis of VL by ELISA (Enzyme Linked ImmunoSorbent Assay) with sera from infected humans and dogs. None of those was valid for the detection of human VL (26-52% sensitivity) although their performance was increased in the canine sera (48-91% sensitivity), with one polypeptide useful for the diagnosis of canine leishmaniasis. Next, we assayed a mixture of three antigens, found to be best for human or canine VL, among 13 identified through different screenings. This "Mix" resulted in similar levels of sensitivity for both human (84%) and canine (88%) sera. With improvements, this validates the use of multiple proteins, including antigens identified here, as components of a single system for the diagnosis of both forms of leishmaniasis.engPublic Library of ScienceLeishmaniasis, VisceralLeishmania infantumProteínas RecombinantesAntígenos, ProtozoáriosSeqüência de aminoácidosAnimaisLeishmaniasis, VisceralLeishmania infantumRecombinant ProteinsAntigens, ProtozoanAmino Acid SequenceAnimalsEvaluation of a new set of recombinant antigens for the serological diagnosis of human and canine visceral leishmaniasisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/22955/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALMagalhaes FB Evaluation of a new....pdfMagalhaes FB Evaluation of a 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| dc.title.pt_BR.fl_str_mv |
Evaluation of a new set of recombinant antigens for the serological diagnosis of human and canine visceral leishmaniasis |
| title |
Evaluation of a new set of recombinant antigens for the serological diagnosis of human and canine visceral leishmaniasis |
| spellingShingle |
Evaluation of a new set of recombinant antigens for the serological diagnosis of human and canine visceral leishmaniasis Magalhães, Franklin Barbalho Leishmaniasis, Visceral Leishmania infantum Proteínas Recombinantes Antígenos, Protozoários Seqüência de aminoácidos Animais Leishmaniasis, Visceral Leishmania infantum Recombinant Proteins Antigens, Protozoan Amino Acid Sequence Animals |
| title_short |
Evaluation of a new set of recombinant antigens for the serological diagnosis of human and canine visceral leishmaniasis |
| title_full |
Evaluation of a new set of recombinant antigens for the serological diagnosis of human and canine visceral leishmaniasis |
| title_fullStr |
Evaluation of a new set of recombinant antigens for the serological diagnosis of human and canine visceral leishmaniasis |
| title_full_unstemmed |
Evaluation of a new set of recombinant antigens for the serological diagnosis of human and canine visceral leishmaniasis |
| title_sort |
Evaluation of a new set of recombinant antigens for the serological diagnosis of human and canine visceral leishmaniasis |
| author |
Magalhães, Franklin Barbalho |
| author_facet |
Magalhães, Franklin Barbalho Castro Neto, Artur Leonel de Nascimento, Marilia B Santos, Wagner José Tenório dos Medeiros, Zulma Maria de Lima Neto, Adelino Soares Costa, Dorcas Lamounier Costa, Carlos H N dosSantos, Washington Luis Conrado Pontes de Carvalho, Lain Carlos Oliveira, Geraldo Gileno de Sá Melo Neto, Osvaldo Pompilio de |
| author_role |
author |
| author2 |
Castro Neto, Artur Leonel de Nascimento, Marilia B Santos, Wagner José Tenório dos Medeiros, Zulma Maria de Lima Neto, Adelino Soares Costa, Dorcas Lamounier Costa, Carlos H N dosSantos, Washington Luis Conrado Pontes de Carvalho, Lain Carlos Oliveira, Geraldo Gileno de Sá Melo Neto, Osvaldo Pompilio de |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Magalhães, Franklin Barbalho Castro Neto, Artur Leonel de Nascimento, Marilia B Santos, Wagner José Tenório dos Medeiros, Zulma Maria de Lima Neto, Adelino Soares Costa, Dorcas Lamounier Costa, Carlos H N dosSantos, Washington Luis Conrado Pontes de Carvalho, Lain Carlos Oliveira, Geraldo Gileno de Sá Melo Neto, Osvaldo Pompilio de |
| dc.subject.other.pt_BR.fl_str_mv |
Leishmaniasis, Visceral Leishmania infantum Proteínas Recombinantes Antígenos, Protozoários Seqüência de aminoácidos Animais |
| topic |
Leishmaniasis, Visceral Leishmania infantum Proteínas Recombinantes Antígenos, Protozoários Seqüência de aminoácidos Animais Leishmaniasis, Visceral Leishmania infantum Recombinant Proteins Antigens, Protozoan Amino Acid Sequence Animals |
| dc.subject.en.pt_BR.fl_str_mv |
Leishmaniasis, Visceral Leishmania infantum Recombinant Proteins Antigens, Protozoan Amino Acid Sequence Animals |
| description |
Brazilian funding agency CNPq and the Brazilian Ministry of Health (Grant MCTI/CNPq/MS-SCTIENo.: 404259/2012). |
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2017 |
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2017-10-25T13:04:19Z |
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2017-10-25T13:04:19Z |
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2017 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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MAGALHÃES, F. B. et al. Evaluation of a new set of recombinant antigens for the serological diagnosis of human and canine visceral leishmaniasis. Plos One, sep. 2017. |
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https://www.arca.fiocruz.br/handle/icict/22955 |
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1932-6203 |
| dc.identifier.doi.none.fl_str_mv |
10.1371/journal.pone.0184867 |
| identifier_str_mv |
MAGALHÃES, F. B. et al. Evaluation of a new set of recombinant antigens for the serological diagnosis of human and canine visceral leishmaniasis. Plos One, sep. 2017. 1932-6203 10.1371/journal.pone.0184867 |
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