Caspar-like gene depletion reduces Leishmania infection in sand fly host Lutzomyia longipalpis

Detalhes bibliográficos
Autor(a) principal: Telleria, Erich L
Data de Publicação: 2012
Outros Autores: Sant'Anna, Maurício R. V., Farias, João R. Ortigão, Pitaluga, André N., Dillon, Viv M., Bates, Paul A., Traub-Csekö, Yara M., Dillon, Rod J.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/15408
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.
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spelling Telleria, Erich LSant'Anna, Maurício R. V.Farias, João R. OrtigãoPitaluga, André N.Dillon, Viv M.Bates, Paul A.Traub-Csekö, Yara M.Dillon, Rod J.2016-08-25T17:59:49Z2016-08-25T17:59:49Z2012TELLERIA, Erich L. et al. Caspar-like Gene Depletion Reduces Leishmania Infection in Sand Fly Host Lutzomyia longipalpis. The Journal of Biological Chemistry, v.287, n.16, p.12985-12993, Apr. 2012.0021-9258https://www.arca.fiocruz.br/handle/icict/1540810.1074/jbc.M111.3315611083-351XengAmerican Society for Biochemistry and Molecular BiologyLutzomyia longipalpisLeishmaniaLutzomyia longipalpisLeishmania InfectionSand Fly HostCaspar-like gene depletion reduces Leishmania infection in sand fly host Lutzomyia longipalpisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Lancaster University. Faculty of Health and Medicine. Lancaster, UK.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.University of Liverpool. Institute of Integrative Biology. Liverpool, UK.Lancaster University. Faculty of Health and Medicine. Lancaster, UK.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Lancaster University. Faculty of Health and Medicine. Lancaster, UK.Female phlebotomine sand flies Lutzomyia longipalpis naturally harbor populations of the medically important Leishmania infantum (syn. Leishmania chagasi) parasite in the gut, but the extent to which the parasite interacts with the immune system of the insect vector is unknown. To investigate the sand fly immune response and its interaction with the Leishmania parasite, we identified a homologue for caspar, a negative regulator of immune deficiency signaling pathway. We found that feeding antibiotics to adult female L. longipalpis resulted in an up-regulation of caspar expression relative to controls. caspar was differentially expressed when females were fed on gram-negative and gram-positive bacterial species. caspar expression was significantly down-regulated in females between 3 and 6 days after a blood feed containing Leishmania mexicana amastigotes. RNA interference was used to deplete caspar expression in female L. longipalpis, which were subsequently fed with Leishmania in a blood meal. Sand fly gut populations of both L. mexicana and L. infantum were significantly reduced in caspar-depleted females. The prevalence of L. infantum infection in the females fell from 85 to 45%. Our results provide the first insight into the operation of immune homeostasis in phlebotomine sand flies during the growth of bacterial and Leishmania populations in the digestive tract. We have demonstrated that the activation of the sand fly immune system, via depletion of a single gene, can lead to the abortion of Leishmania development and the disruption of transmission by the phlebotomine sand fly.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/15408/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALerich_telleria_etal_IOC_2012.pdferich_telleria_etal_IOC_2012.pdfapplication/pdf2492480https://www.arca.fiocruz.br/bitstream/icict/15408/2/erich_telleria_etal_IOC_2012.pdff645df0b6201748b6b3052182e6cf30fMD52TEXTerich_telleria_etal_IOC_2012.pdf.txterich_telleria_etal_IOC_2012.pdf.txtExtracted texttext/plain51914https://www.arca.fiocruz.br/bitstream/icict/15408/3/erich_telleria_etal_IOC_2012.pdf.txt7a33e2ea472802418f1a0550d4b2a9e4MD53icict/154082018-04-02 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dc.title.pt_BR.fl_str_mv Caspar-like gene depletion reduces Leishmania infection in sand fly host Lutzomyia longipalpis
title Caspar-like gene depletion reduces Leishmania infection in sand fly host Lutzomyia longipalpis
spellingShingle Caspar-like gene depletion reduces Leishmania infection in sand fly host Lutzomyia longipalpis
Telleria, Erich L
Lutzomyia longipalpis
Leishmania
Lutzomyia longipalpis
Leishmania Infection
Sand Fly Host
title_short Caspar-like gene depletion reduces Leishmania infection in sand fly host Lutzomyia longipalpis
title_full Caspar-like gene depletion reduces Leishmania infection in sand fly host Lutzomyia longipalpis
title_fullStr Caspar-like gene depletion reduces Leishmania infection in sand fly host Lutzomyia longipalpis
title_full_unstemmed Caspar-like gene depletion reduces Leishmania infection in sand fly host Lutzomyia longipalpis
title_sort Caspar-like gene depletion reduces Leishmania infection in sand fly host Lutzomyia longipalpis
author Telleria, Erich L
author_facet Telleria, Erich L
Sant'Anna, Maurício R. V.
Farias, João R. Ortigão
Pitaluga, André N.
Dillon, Viv M.
Bates, Paul A.
Traub-Csekö, Yara M.
Dillon, Rod J.
author_role author
author2 Sant'Anna, Maurício R. V.
Farias, João R. Ortigão
Pitaluga, André N.
Dillon, Viv M.
Bates, Paul A.
Traub-Csekö, Yara M.
Dillon, Rod J.
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Telleria, Erich L
Sant'Anna, Maurício R. V.
Farias, João R. Ortigão
Pitaluga, André N.
Dillon, Viv M.
Bates, Paul A.
Traub-Csekö, Yara M.
Dillon, Rod J.
dc.subject.other.pt_BR.fl_str_mv Lutzomyia longipalpis
Leishmania
topic Lutzomyia longipalpis
Leishmania
Lutzomyia longipalpis
Leishmania Infection
Sand Fly Host
dc.subject.en.pt_BR.fl_str_mv Lutzomyia longipalpis
Leishmania Infection
Sand Fly Host
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.
publishDate 2012
dc.date.issued.fl_str_mv 2012
dc.date.accessioned.fl_str_mv 2016-08-25T17:59:49Z
dc.date.available.fl_str_mv 2016-08-25T17:59:49Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv TELLERIA, Erich L. et al. Caspar-like Gene Depletion Reduces Leishmania Infection in Sand Fly Host Lutzomyia longipalpis. The Journal of Biological Chemistry, v.287, n.16, p.12985-12993, Apr. 2012.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/15408
dc.identifier.issn.pt_BR.fl_str_mv 0021-9258
dc.identifier.doi.none.fl_str_mv 10.1074/jbc.M111.331561
dc.identifier.eissn.none.fl_str_mv 1083-351X
identifier_str_mv TELLERIA, Erich L. et al. Caspar-like Gene Depletion Reduces Leishmania Infection in Sand Fly Host Lutzomyia longipalpis. The Journal of Biological Chemistry, v.287, n.16, p.12985-12993, Apr. 2012.
0021-9258
10.1074/jbc.M111.331561
1083-351X
url https://www.arca.fiocruz.br/handle/icict/15408
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Society for Biochemistry and Molecular Biology
publisher.none.fl_str_mv American Society for Biochemistry and Molecular Biology
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/15408/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/15408/2/erich_telleria_etal_IOC_2012.pdf
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