Transcriptional profiles of SHH pathway genes in keratocystic odontogenic tumor and ameloblastoma.
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2014 |
| Outros Autores: | , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/8469 |
Resumo: | Federal University of Bahia. School of Dentistry. Salvador, BA, Brasil |
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Gurgel, Clarissa Araújo SilvaBuim, Marcilei Eliza CavichiolliCarvalho, Katia CândidoSales, Caroline Brandi SchlaepferReis, Mitermayer Galvão dosSouza, Renata Oliveira deValverde, Ludmila de FaroAzevedo, Roberto Almeida deSantos, Jean Nunes dosSoares, Fernando AugustoRamos, Eduardo Antônio Gonçalves2014-09-29T18:46:06Z2014-09-29T18:46:06Z2014SANTOS, J. N. et al. Transcriptional profiles of SHH pathway genes in keratocystic odontogenic tumor and ameloblastoma. Journal of Oral Pathology & Medicine, v. 43, n. 8, p. 619-626, 2014.0904-2512https://www.arca.fiocruz.br/handle/icict/846910.1111/jop.12180engJohn Wiley & Sons, LtdTranscriptional profiles of SHH pathway genes in keratocystic odontogenic tumor and ameloblastoma.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFederal University of Bahia. School of Dentistry. Salvador, BA, BrasilAC Camargo Cancer Center. São Paulo, SP, BrasilUniversity of São Paulo. School of Medicine. São Paulo, SP, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, BrasilFederal University of Bahia. School of Dentistry. Salvador, BA, BrasilFederal University of Bahia. School of Dentistry. Salvador, BA, BrasilAC Camargo Cancer Center. São Paulo, SP, Brasil / University of São Paulo. School of Dentistry. São Paulo, SP, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, BrasilBACKGROUND: Sonic hedgehog (SHH) pathway activation has been identified as a key factor in the development of many types of tumors, including odontogenic tumors. Our study examined the expression of genes in the SHH pathway to characterize their roles in the pathogenesis of keratocystic odontogenic tumors (KOT) and ameloblastomas (AB). METHODS: We quantified the expression of SHH, SMO, PTCH1, SUFU, GLI1, CCND1, and BCL2 genes by qPCR in a total of 23 KOT, 11 AB, and three non-neoplastic oral mucosa (NNM). We also measured the expression of proteins related to this pathway (CCND1 and BCL2) by immunohistochemistry. RESULTS: Weobserved overexpression of SMO, PTCH1, GLI1, and CCND1 genes in both KOT (23/23) and AB (11/ 11). However, we did not detect expression of the SHH gene in 21/23 KOT and 10/11 AB tumors. Low levels of the SUFU gene were expressed in KOT (P = 0.0199) and AB (P = 0.0127) relative to the NNM. Recurrent KOT exhibited high levels of SMO (P = 0.035), PTCH1 (P = 0.048), CCND1 (P = 0.048), and BCL2 (P = 0.045) transcripts. Using immunolabeling of CCND1, we observed no statistical difference between primary and recurrent KOT (P = 0.8815), sporadic and NBCCS-KOT (P = 0.7688), and unicystic and solid AB (P = 0.7521). CONCLUSIONS: Overexpression of upstream (PTCH1 and SMO) and downstream (GLI1, CCND1 and BCL2) genes in the SHH pathway leads to the constitutive activation of this pathway in KOT and AB and may suggest a mechanism for the development of these types of tumors.AmeloblastomaKeratocystic odontogenic tumorOdontogenic tumorsSonic hedgehoginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/8469/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALSantos JN Transcriptional....pdfSantos JN Transcriptional....pdfapplication/pdf447396https://www.arca.fiocruz.br/bitstream/icict/8469/2/Santos%20JN%20Transcriptional....pdf2037a40e0a2e5f6a9e4165e4175045deMD52TEXTSantos JN Transcriptional....pdf.txtSantos JN Transcriptional....pdf.txtExtracted texttext/plain38665https://www.arca.fiocruz.br/bitstream/icict/8469/3/Santos%20JN%20Transcriptional....pdf.txte6f6281220b6867f3186cf7815dd85efMD53icict/84692023-03-15 14:33:24.514oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:24Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Transcriptional profiles of SHH pathway genes in keratocystic odontogenic tumor and ameloblastoma. |
| title |
Transcriptional profiles of SHH pathway genes in keratocystic odontogenic tumor and ameloblastoma. |
| spellingShingle |
Transcriptional profiles of SHH pathway genes in keratocystic odontogenic tumor and ameloblastoma. Gurgel, Clarissa Araújo Silva Ameloblastoma Keratocystic odontogenic tumor Odontogenic tumors Sonic hedgehog |
| title_short |
Transcriptional profiles of SHH pathway genes in keratocystic odontogenic tumor and ameloblastoma. |
| title_full |
Transcriptional profiles of SHH pathway genes in keratocystic odontogenic tumor and ameloblastoma. |
| title_fullStr |
Transcriptional profiles of SHH pathway genes in keratocystic odontogenic tumor and ameloblastoma. |
| title_full_unstemmed |
Transcriptional profiles of SHH pathway genes in keratocystic odontogenic tumor and ameloblastoma. |
| title_sort |
Transcriptional profiles of SHH pathway genes in keratocystic odontogenic tumor and ameloblastoma. |
| author |
Gurgel, Clarissa Araújo Silva |
| author_facet |
Gurgel, Clarissa Araújo Silva Buim, Marcilei Eliza Cavichiolli Carvalho, Katia Cândido Sales, Caroline Brandi Schlaepfer Reis, Mitermayer Galvão dos Souza, Renata Oliveira de Valverde, Ludmila de Faro Azevedo, Roberto Almeida de Santos, Jean Nunes dos Soares, Fernando Augusto Ramos, Eduardo Antônio Gonçalves |
| author_role |
author |
| author2 |
Buim, Marcilei Eliza Cavichiolli Carvalho, Katia Cândido Sales, Caroline Brandi Schlaepfer Reis, Mitermayer Galvão dos Souza, Renata Oliveira de Valverde, Ludmila de Faro Azevedo, Roberto Almeida de Santos, Jean Nunes dos Soares, Fernando Augusto Ramos, Eduardo Antônio Gonçalves |
| author2_role |
author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Gurgel, Clarissa Araújo Silva Buim, Marcilei Eliza Cavichiolli Carvalho, Katia Cândido Sales, Caroline Brandi Schlaepfer Reis, Mitermayer Galvão dos Souza, Renata Oliveira de Valverde, Ludmila de Faro Azevedo, Roberto Almeida de Santos, Jean Nunes dos Soares, Fernando Augusto Ramos, Eduardo Antônio Gonçalves |
| dc.subject.en.pt_BR.fl_str_mv |
Ameloblastoma Keratocystic odontogenic tumor Odontogenic tumors Sonic hedgehog |
| topic |
Ameloblastoma Keratocystic odontogenic tumor Odontogenic tumors Sonic hedgehog |
| description |
Federal University of Bahia. School of Dentistry. Salvador, BA, Brasil |
| publishDate |
2014 |
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2014-09-29T18:46:06Z |
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2014-09-29T18:46:06Z |
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2014 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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SANTOS, J. N. et al. Transcriptional profiles of SHH pathway genes in keratocystic odontogenic tumor and ameloblastoma. Journal of Oral Pathology & Medicine, v. 43, n. 8, p. 619-626, 2014. |
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https://www.arca.fiocruz.br/handle/icict/8469 |
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0904-2512 |
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10.1111/jop.12180 |
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SANTOS, J. N. et al. Transcriptional profiles of SHH pathway genes in keratocystic odontogenic tumor and ameloblastoma. Journal of Oral Pathology & Medicine, v. 43, n. 8, p. 619-626, 2014. 0904-2512 10.1111/jop.12180 |
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