A lactate dehydrogenase ELISA-based assay for the in vitro determination of Plasmodium berghei sensitivity to anti-malarial drugs

Detalhes bibliográficos
Autor(a) principal: Orjuela-Sánchez, Pamela
Data de Publicação: 2012
Outros Autores: Duggan, Erika, Nolan, John, Frangos, John A., Carvalho, Leonardo J. M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/18497
Resumo: La Jolla Bioengineering Institute. San Diego, CA, USA.
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spelling Orjuela-Sánchez, PamelaDuggan, ErikaNolan, JohnFrangos, John A.Carvalho, Leonardo J. M.2017-04-18T16:07:33Z2017-04-18T16:07:33Z2012ORJUELA-SANCHEZ, Pamela; et al. A lactate dehydrogenase ELISA-based assay for the in vitro determination of Plasmodium berghei sensitivity to anti-malarial drugs. Malaria Journal, v.11:366, 8p, Nov. 2012.1475-287https://www.arca.fiocruz.br/handle/icict/1849710.1186/1475-2875-11-366engBioMed CentralPlasmodium bergheiCultura in vitroInfecção sincronizadaEnsaio in vitro com fármacoVacinas AntimaláricasL-Lactato DesidrogenaseEnsaio de Imunoadsorção EnzimáticaIn vitro cultureschizontsynchronized infectiondrug in vitro assayanti-malarialsIC50 valueslactate dehydrogenaseELISAPlasmodium bergheiA lactate dehydrogenase ELISA-based assay for the in vitro determination of Plasmodium berghei sensitivity to anti-malarial drugsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleLa Jolla Bioengineering Institute. San Diego, CA, USA.La Jolla Bioengineering Institute. San Diego, CA, USA.La Jolla Bioengineering Institute. San Diego, CA, USA.La Jolla Bioengineering Institute. San Diego, CA, USA.La Jolla Bioengineering Institute. San Diego, CA, USA / Fundação Oswaldo Cruz. Instituto Oswaldo cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil.Plasmodium berghei rodent malaria is a well-known model for the investigation of anti-malarial drug efficacy in vivo. However, the availability of drug in vitro assays in P. berghei is reduced when compared with the spectrum of techniques existing for Plasmodium falciparum. New alternatives to the current manual or automated methods described for P. berghei are attractive. The present study reports a new ELISA drug in vitro assay for P. berghei using two monoclonal antibodies against the parasite lactate dehydrogenase (pLDH).info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/18497/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALleonardo3_carvalho_etal_IOC_2012.pdfleonardo3_carvalho_etal_IOC_2012.pdfapplication/pdf555462https://www.arca.fiocruz.br/bitstream/icict/18497/2/leonardo3_carvalho_etal_IOC_2012.pdff9171db08378d090a8f40478c578ea44MD52TEXTleonardo3_carvalho_etal_IOC_2012.pdf.txtleonardo3_carvalho_etal_IOC_2012.pdf.txtExtracted texttext/plain40745https://www.arca.fiocruz.br/bitstream/icict/18497/3/leonardo3_carvalho_etal_IOC_2012.pdf.txta6739311e663bb1e505ccf9ac895e69dMD53icict/184972018-08-15 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dc.title.pt_BR.fl_str_mv A lactate dehydrogenase ELISA-based assay for the in vitro determination of Plasmodium berghei sensitivity to anti-malarial drugs
title A lactate dehydrogenase ELISA-based assay for the in vitro determination of Plasmodium berghei sensitivity to anti-malarial drugs
spellingShingle A lactate dehydrogenase ELISA-based assay for the in vitro determination of Plasmodium berghei sensitivity to anti-malarial drugs
Orjuela-Sánchez, Pamela
Plasmodium berghei
Cultura in vitro
Infecção sincronizada
Ensaio in vitro com fármaco
Vacinas Antimaláricas
L-Lactato Desidrogenase
Ensaio de Imunoadsorção Enzimática
In vitro culture
schizont
synchronized infection
drug in vitro assay
anti-malarials
IC50 values
lactate dehydrogenase
ELISA
Plasmodium berghei
title_short A lactate dehydrogenase ELISA-based assay for the in vitro determination of Plasmodium berghei sensitivity to anti-malarial drugs
title_full A lactate dehydrogenase ELISA-based assay for the in vitro determination of Plasmodium berghei sensitivity to anti-malarial drugs
title_fullStr A lactate dehydrogenase ELISA-based assay for the in vitro determination of Plasmodium berghei sensitivity to anti-malarial drugs
title_full_unstemmed A lactate dehydrogenase ELISA-based assay for the in vitro determination of Plasmodium berghei sensitivity to anti-malarial drugs
title_sort A lactate dehydrogenase ELISA-based assay for the in vitro determination of Plasmodium berghei sensitivity to anti-malarial drugs
author Orjuela-Sánchez, Pamela
author_facet Orjuela-Sánchez, Pamela
Duggan, Erika
Nolan, John
Frangos, John A.
Carvalho, Leonardo J. M.
author_role author
author2 Duggan, Erika
Nolan, John
Frangos, John A.
Carvalho, Leonardo J. M.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Orjuela-Sánchez, Pamela
Duggan, Erika
Nolan, John
Frangos, John A.
Carvalho, Leonardo J. M.
dc.subject.other.pt_BR.fl_str_mv Plasmodium berghei
Cultura in vitro
Infecção sincronizada
Ensaio in vitro com fármaco
Vacinas Antimaláricas
L-Lactato Desidrogenase
Ensaio de Imunoadsorção Enzimática
topic Plasmodium berghei
Cultura in vitro
Infecção sincronizada
Ensaio in vitro com fármaco
Vacinas Antimaláricas
L-Lactato Desidrogenase
Ensaio de Imunoadsorção Enzimática
In vitro culture
schizont
synchronized infection
drug in vitro assay
anti-malarials
IC50 values
lactate dehydrogenase
ELISA
Plasmodium berghei
dc.subject.en.pt_BR.fl_str_mv In vitro culture
schizont
synchronized infection
drug in vitro assay
anti-malarials
IC50 values
lactate dehydrogenase
ELISA
Plasmodium berghei
description La Jolla Bioengineering Institute. San Diego, CA, USA.
publishDate 2012
dc.date.issued.fl_str_mv 2012
dc.date.accessioned.fl_str_mv 2017-04-18T16:07:33Z
dc.date.available.fl_str_mv 2017-04-18T16:07:33Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ORJUELA-SANCHEZ, Pamela; et al. A lactate dehydrogenase ELISA-based assay for the in vitro determination of Plasmodium berghei sensitivity to anti-malarial drugs. Malaria Journal, v.11:366, 8p, Nov. 2012.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/18497
dc.identifier.issn.pt_BR.fl_str_mv 1475-287
dc.identifier.doi.none.fl_str_mv 10.1186/1475-2875-11-366
identifier_str_mv ORJUELA-SANCHEZ, Pamela; et al. A lactate dehydrogenase ELISA-based assay for the in vitro determination of Plasmodium berghei sensitivity to anti-malarial drugs. Malaria Journal, v.11:366, 8p, Nov. 2012.
1475-287
10.1186/1475-2875-11-366
url https://www.arca.fiocruz.br/handle/icict/18497
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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https://www.arca.fiocruz.br/bitstream/icict/18497/3/leonardo3_carvalho_etal_IOC_2012.pdf.txt
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